Taking Terminalia chebula Retz. as the control product, the polyphenolic contents of the processed products in Tibetian, Rubia cordifolia L. processed Terminalia chebula Retz. and Euphorbia fischeriana Steud processed...Taking Terminalia chebula Retz. as the control product, the polyphenolic contents of the processed products in Tibetian, Rubia cordifolia L. processed Terminalia chebula Retz. and Euphorbia fischeriana Steud processed Terminalia chebula Retz. were analyzed by HPLC. Polyphenolic contents increased from 4.54% to 5.69% and 7.46%, respectively, which may lead to the change in their pharmacological effects.展开更多
To study the change of the content of chebulinic acid in Terminalia chebula Retz. by different processing methods in Tibet. We taking chebulinic acid as an indicator, the contents in Terminalia chebula Retz. and its p...To study the change of the content of chebulinic acid in Terminalia chebula Retz. by different processing methods in Tibet. We taking chebulinic acid as an indicator, the contents in Terminalia chebula Retz. and its processed products were analyzed by using HPLC. Also, the change rule was explored. Compared with Terminalia chebula Retz., the contents of chebulinic acid have decreased after processing, declining 50.18% for the Rubia cordifolia L. processed product, while 11.2% reduce for the Euphorbia fischeriana Steud processed product. After the specific preparation process in Tibet, the contents of chebulinic acid have reduced significantly, which may lead to different pharmacological effects.展开更多
Background:Based on modern pharmacological studies,Terminalia chebula Retz.exhibits antimicrobial activity against a variety of bacteria.Previously,we found Terminalia chebula Retz.exhibited excellent antibacterial ac...Background:Based on modern pharmacological studies,Terminalia chebula Retz.exhibits antimicrobial activity against a variety of bacteria.Previously,we found Terminalia chebula Retz.exhibited excellent antibacterial activity against Malassezia restricta.Methods:We determined the minimal inhibitory concentration(MIC)and minimum bactericidal concentration of Terminalia chebula Retz.extraction with water(TRW)against Staphylococcus epidermidis(including Staphylococcus epidermidis 1-15)using the microdilution method.Staphylococcus epidermidis 1(SE11),which was the most sensitive to TRW,was selected as the test bacterium for subsequent experiments.The time-kill curve of TRW on SE11 was generated using the viable count method.Further,an in vitro biofilm model of SE11 was constructed using the 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-((phenylamino)carbonyl)-2H-tetrazolium hydroxide test,semi-quantitative crystal violet experiment,and scanning electron microscopy.The effects of TRW on the total amount of biofilm formation,the number of viable biofilm bacteria,and biofilm microstructure of SE11 were also determined using a semi-quantitative crystal violet experiment,viable count method,and scanning electron microscopy.Finally,the electrical conductivity and soluble protein content of the SE11 suspensions were determined.Results:The average MIC of TRW against SE11 was 0.75±1.09 mg/mL.TRW(1/2MIC and 2MIC)and zinc pyrithione(1/2MIC and 2MIC)had no significant effect on total biofilm inhibition in the adhesion stage(P>0.05)and the aggregation stage(P>0.05).Further,TRW(1/2MIC and MIC)and zinc pyrithione(1/2MIC and MIC)had no significant effect on viable biofilm bacteria in the adhesion stage(P>0.05)and aggregation stage(P>0.05).TRW destroyed the integrity of the SE11 cell membrane,resulting in leakage of intracellular substances.Conclusion:TRW inhibits SE11 biofilm formation and is similar to zinc pyrithione in the adhesion and aggregation stages,which provides a theoretical basis for its application in the field of antimicrobial additives.展开更多
In order to investigate the chemical-biological relationship caused by the special processing technology,the constituent＇s content and antioxidant capacity in vivo of Euphorbia fischeriana Steud processing Terminalia...In order to investigate the chemical-biological relationship caused by the special processing technology,the constituent＇s content and antioxidant capacity in vivo of Euphorbia fischeriana Steud processing Terminalia chebula Retz. have been tested. Taking T. chebula Retz. as control,the content of gallic acid is analyzed with high performance liquid chromatography（HPLC） method. The results show that the increase of gallic acid concentration from 4.54% to 7.46% leads to enhancement of the antioxidant capacities in vivo of the E. fischeriana Steud processing T. chebula Retz. The significant antioxidant capacity in vivo（p〈0.05） of the processed product possesses is better than the unprocessed herb. Its superoxide dismutase（SOD）and malondia-ldehyde（MDA） contents in serum,the MDA content in liver can match the effects of vitamine E when administered at high dosage.展开更多
以传统药用植物意大利牛舌草(Anchusa italica Retz.)的临床功效和现代药理学研究为基础,通过构建体外抑郁症模型,系统研究牛舌草的药理作用及物质基础.HPLC检测不同产地(新疆、巴基斯坦、海南)牛舌草总黄酮(TFAI)的化学成分;然后用海...以传统药用植物意大利牛舌草(Anchusa italica Retz.)的临床功效和现代药理学研究为基础,通过构建体外抑郁症模型,系统研究牛舌草的药理作用及物质基础.HPLC检测不同产地(新疆、巴基斯坦、海南)牛舌草总黄酮(TFAI)的化学成分;然后用海马细胞(HT-22)通过皮质酮诱导建立神经细胞损伤模型,MTT法检测了5种牛舌草对HT-22细胞活力的影响,并计算其EC_(50).结果表明不同产地TFAI在500µg/mL浓度下对HT-22细胞均未表现出明显的细胞毒性作用,且对HT-22细胞具有显著的增殖作用,对过量皮质酮诱导的海马细胞损伤具有明显的细胞保护作用,比较5批牛舌草中的总黄酮含量和EC_(50)值,海南产地的牛舌草中的黄酮类含量最高,且对HT-22细胞的增殖作用EC_(50)值最低,具有较高的效价.研究结果说明五批TFAI提取物对皮质酮诱导的HT-22海马细胞损伤均具有保护作用,可以不同程度地增加HT-22细胞活力,具有显著的神经保护作用.展开更多
[目的]筛选高效防控水利枢纽坝区恶性杂草斑茅的除草剂。[方法]采用室内生物测定试验和田间药效试验评估了5种除草剂单剂及其与草甘膦异丙胺盐混配对斑茅的防控效果。[结果]室内生物活性测定结果表明,11%精草铵膦铵盐可溶液剂712.5 g a....[目的]筛选高效防控水利枢纽坝区恶性杂草斑茅的除草剂。[方法]采用室内生物测定试验和田间药效试验评估了5种除草剂单剂及其与草甘膦异丙胺盐混配对斑茅的防控效果。[结果]室内生物活性测定结果表明,11%精草铵膦铵盐可溶液剂712.5 g a.i./hm^(2)对斑茅的株防效达90%,优于其他单剂。田间药效试验显示,75%甲嘧磺隆水分散粒剂1012.5 g a.i./hm^(2)与41%草甘膦异丙胺盐水剂2306.25 g a.i./hm^(2)混配对斑茅的防效超80%、持效期达90 d。[结论]75%甲嘧磺隆水分散粒剂与41%草甘膦异丙胺盐水剂混配对斑茅的防控效果优异,为高效防控斑茅提供了有效的策略。展开更多
对诃子抗氧化活性物质提取工艺、抗氧化活性及其初步物质基础进行了研究。结果表明,最佳提取工艺为:乙醇体积分数50%、液料比20、提取温度60℃、提取次数4次、提取时间7 m in;总还原力、FRAP法抗氧化能力和DPPH自由基清除能力的结果均...对诃子抗氧化活性物质提取工艺、抗氧化活性及其初步物质基础进行了研究。结果表明,最佳提取工艺为:乙醇体积分数50%、液料比20、提取温度60℃、提取次数4次、提取时间7 m in;总还原力、FRAP法抗氧化能力和DPPH自由基清除能力的结果均显示诃子粗提物具有很强的抗氧化活性。生物活性追踪结果发现,诃子抗氧化活性物质主要由存在于乙酸乙酯相的弱极性化合物组成,该相质量浓度为25、50μg/mL样品的总还原力OD700值分别为0.197±0.002和0.380±0.006,FRAP法抗氧化能力OD593分别为0.272±0.023和0.631±0.002,DPPH自由基清除率分别为(86.838±0.600)%和(90.318±0.917)%。相关关系表明,诃子抗氧化活性的主要物质基础为总黄酮和总多酚。展开更多
基金The Research Platform for the Project of the Application Basic Plan in Sichuan Province(Grant No.2014JY0113)the Project of Department of Science in Sichuan Province(Grant No.14ZA0003)
文摘Taking Terminalia chebula Retz. as the control product, the polyphenolic contents of the processed products in Tibetian, Rubia cordifolia L. processed Terminalia chebula Retz. and Euphorbia fischeriana Steud processed Terminalia chebula Retz. were analyzed by HPLC. Polyphenolic contents increased from 4.54% to 5.69% and 7.46%, respectively, which may lead to the change in their pharmacological effects.
基金The Research Platform for the Project of the Application Basic Plan in Sichuan Province(Grant No.2014JY0113)the Project of Department of Science in Sichuan Province(Grant No.14ZA0003)
文摘To study the change of the content of chebulinic acid in Terminalia chebula Retz. by different processing methods in Tibet. We taking chebulinic acid as an indicator, the contents in Terminalia chebula Retz. and its processed products were analyzed by using HPLC. Also, the change rule was explored. Compared with Terminalia chebula Retz., the contents of chebulinic acid have decreased after processing, declining 50.18% for the Rubia cordifolia L. processed product, while 11.2% reduce for the Euphorbia fischeriana Steud processed product. After the specific preparation process in Tibet, the contents of chebulinic acid have reduced significantly, which may lead to different pharmacological effects.
文摘Background:Based on modern pharmacological studies,Terminalia chebula Retz.exhibits antimicrobial activity against a variety of bacteria.Previously,we found Terminalia chebula Retz.exhibited excellent antibacterial activity against Malassezia restricta.Methods:We determined the minimal inhibitory concentration(MIC)and minimum bactericidal concentration of Terminalia chebula Retz.extraction with water(TRW)against Staphylococcus epidermidis(including Staphylococcus epidermidis 1-15)using the microdilution method.Staphylococcus epidermidis 1(SE11),which was the most sensitive to TRW,was selected as the test bacterium for subsequent experiments.The time-kill curve of TRW on SE11 was generated using the viable count method.Further,an in vitro biofilm model of SE11 was constructed using the 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-((phenylamino)carbonyl)-2H-tetrazolium hydroxide test,semi-quantitative crystal violet experiment,and scanning electron microscopy.The effects of TRW on the total amount of biofilm formation,the number of viable biofilm bacteria,and biofilm microstructure of SE11 were also determined using a semi-quantitative crystal violet experiment,viable count method,and scanning electron microscopy.Finally,the electrical conductivity and soluble protein content of the SE11 suspensions were determined.Results:The average MIC of TRW against SE11 was 0.75±1.09 mg/mL.TRW(1/2MIC and 2MIC)and zinc pyrithione(1/2MIC and 2MIC)had no significant effect on total biofilm inhibition in the adhesion stage(P>0.05)and the aggregation stage(P>0.05).Further,TRW(1/2MIC and MIC)and zinc pyrithione(1/2MIC and MIC)had no significant effect on viable biofilm bacteria in the adhesion stage(P>0.05)and aggregation stage(P>0.05).TRW destroyed the integrity of the SE11 cell membrane,resulting in leakage of intracellular substances.Conclusion:TRW inhibits SE11 biofilm formation and is similar to zinc pyrithione in the adhesion and aggregation stages,which provides a theoretical basis for its application in the field of antimicrobial additives.
基金Suppported by the Research Platform for the Project of the Application Basic Plan in Sichuan Province(2014JY0113)the Project of Department of science in Sichuan Province(14ZA0003)
文摘In order to investigate the chemical-biological relationship caused by the special processing technology,the constituent＇s content and antioxidant capacity in vivo of Euphorbia fischeriana Steud processing Terminalia chebula Retz. have been tested. Taking T. chebula Retz. as control,the content of gallic acid is analyzed with high performance liquid chromatography（HPLC） method. The results show that the increase of gallic acid concentration from 4.54% to 7.46% leads to enhancement of the antioxidant capacities in vivo of the E. fischeriana Steud processing T. chebula Retz. The significant antioxidant capacity in vivo（p〈0.05） of the processed product possesses is better than the unprocessed herb. Its superoxide dismutase（SOD）and malondia-ldehyde（MDA） contents in serum,the MDA content in liver can match the effects of vitamine E when administered at high dosage.
文摘以传统药用植物意大利牛舌草(Anchusa italica Retz.)的临床功效和现代药理学研究为基础,通过构建体外抑郁症模型,系统研究牛舌草的药理作用及物质基础.HPLC检测不同产地(新疆、巴基斯坦、海南)牛舌草总黄酮(TFAI)的化学成分;然后用海马细胞(HT-22)通过皮质酮诱导建立神经细胞损伤模型,MTT法检测了5种牛舌草对HT-22细胞活力的影响,并计算其EC_(50).结果表明不同产地TFAI在500µg/mL浓度下对HT-22细胞均未表现出明显的细胞毒性作用,且对HT-22细胞具有显著的增殖作用,对过量皮质酮诱导的海马细胞损伤具有明显的细胞保护作用,比较5批牛舌草中的总黄酮含量和EC_(50)值,海南产地的牛舌草中的黄酮类含量最高,且对HT-22细胞的增殖作用EC_(50)值最低,具有较高的效价.研究结果说明五批TFAI提取物对皮质酮诱导的HT-22海马细胞损伤均具有保护作用,可以不同程度地增加HT-22细胞活力,具有显著的神经保护作用.
文摘[目的]筛选高效防控水利枢纽坝区恶性杂草斑茅的除草剂。[方法]采用室内生物测定试验和田间药效试验评估了5种除草剂单剂及其与草甘膦异丙胺盐混配对斑茅的防控效果。[结果]室内生物活性测定结果表明,11%精草铵膦铵盐可溶液剂712.5 g a.i./hm^(2)对斑茅的株防效达90%,优于其他单剂。田间药效试验显示,75%甲嘧磺隆水分散粒剂1012.5 g a.i./hm^(2)与41%草甘膦异丙胺盐水剂2306.25 g a.i./hm^(2)混配对斑茅的防效超80%、持效期达90 d。[结论]75%甲嘧磺隆水分散粒剂与41%草甘膦异丙胺盐水剂混配对斑茅的防控效果优异,为高效防控斑茅提供了有效的策略。
文摘对诃子抗氧化活性物质提取工艺、抗氧化活性及其初步物质基础进行了研究。结果表明,最佳提取工艺为:乙醇体积分数50%、液料比20、提取温度60℃、提取次数4次、提取时间7 m in;总还原力、FRAP法抗氧化能力和DPPH自由基清除能力的结果均显示诃子粗提物具有很强的抗氧化活性。生物活性追踪结果发现,诃子抗氧化活性物质主要由存在于乙酸乙酯相的弱极性化合物组成,该相质量浓度为25、50μg/mL样品的总还原力OD700值分别为0.197±0.002和0.380±0.006,FRAP法抗氧化能力OD593分别为0.272±0.023和0.631±0.002,DPPH自由基清除率分别为(86.838±0.600)%和(90.318±0.917)%。相关关系表明,诃子抗氧化活性的主要物质基础为总黄酮和总多酚。
