We report the development of a triplex nucleic acid lateral flow immunoassay(NALFIA)for the detection of the genomes of Nipah virus(NiV),Middle East respiratory syndrome coronavirus(MERS-CoV)and Reston ebolavirus(REBO...We report the development of a triplex nucleic acid lateral flow immunoassay(NALFIA)for the detection of the genomes of Nipah virus(NiV),Middle East respiratory syndrome coronavirus(MERS-CoV)and Reston ebolavirus(REBOV),which are intended for screening bats as well as other hosts and reservoirs of these three viruses.Our triplex NALFIA is a two-step assay format:the target nucleic acid in the sample is first amplified using tagged primers,and the tagged dsDNA amplicons are captured by antibodies immobilized on the NALFIA device,resulting in signal development from the binding of a streptavidin-colloidal gold conjugate to a biotin tag on the captured amplicons.Triplex amplification of the N gene of NiV,the UpE gene of MERS-CoV,and the Vp40 gene of REBOV was optimized,and three compatible combinations of hapten labels and antibodies were identified for end point detection.The lowest RNA copy numbers detected by the triplex NALFIA were 8.21e4 for the NiV N target,7.09e1 for the MERS-CoV UpE target,and 1.83e4 for the REBOV Vp40 target.Using simulated samples,the sensitivity and specificity for MERS-CoV and REBOV targets were estimated to be 100%,while the sensitivity and specificity for the NiV target were 91%and 93.3%,respectively.The compliance rate between triplex NALFIA and real-time RT‒PCR was 92%for the NiV N target and 100%for the MERS-CoV UpE and REBOV Vp40 targets.展开更多
Based on TBB data from Meteorological Institute Research of Japan, study is carried out of the features of seasonal transition of Asian-Australian monsoons and Asian summer monsoon establishment,indicating that the tr...Based on TBB data from Meteorological Institute Research of Japan, study is carried out of the features of seasonal transition of Asian-Australian monsoons and Asian summer monsoon establishment,indicating that the transition begins as early as in April, followed by abrupt change in May-June; the Asian summer monsoon situation is fully established in June. The winter convective center in Sumatra moved steadily northwestward across the "land bridge" of the maritime continent and the Indo-China Peninsula as time goes from winter to summer, thus giving rise to the change in large scale circulations that is responsible for the summer monsoon establishment over SE Asia and India; the South China Sea to the western Pacific summer monsoon onset bears a close relation to the active convection in the Indo China Peninsula and steady eastward retreat of the subtropical TBB high-value band,corresponding to the western Pacific subtropical high.展开更多
This article details a public acupuncture demonstration that took place on May 9,1972,in San Francisco,California.It was conducted by two traditional Chinese medicine doctors Pien Bae Chi(卞伯岐)and Leung Kok Yuen(梁...This article details a public acupuncture demonstration that took place on May 9,1972,in San Francisco,California.It was conducted by two traditional Chinese medicine doctors Pien Bae Chi(卞伯岐)and Leung Kok Yuen(梁觉玄),and was observed by 500 American medical doctors.This was an important public acupuncture demonstration in the early history of acupunccture in the United States(US).It directly promoted the passage of the first acupuncture bill by California government and had a significant impact on the legalization of acupuncture in California.展开更多
It has historically been very difficult to trace the history of the westward transmission of Chinese medicine through the accounts of its protagonists. Many of the early scholars such as Jack Worsley, Dick Van Buren, ...It has historically been very difficult to trace the history of the westward transmission of Chinese medicine through the accounts of its protagonists. Many of the early scholars such as Jack Worsley, Dick Van Buren, and Joe Goodman were reluctant to divulge information about the source of their knowledge, or their professional qualifications. Others, such as John Shen and Hong Yuan-bain were early 20th century immigrants who transmitted highly personalized versions of acupuncture and Chinese medicine to select disciples. Eventually, a new class of scholars appeared, including names such as Ted Kaptchuk, Peter Deadman, Nigel Wiseman, William Morris, Peter Eckman, John Mc Donald, Charles Buck, and the late Giovanni Maciocia who looked for answers back in China, developed translation methodologies and terminology, compiled the main textbooks currently in use at TCM colleges, overcame enormous scholastic adversity, developed courses and pursued the regulation and accreditation of TCM in various countries. This special issue synopsizes the path towards the global acculturation of TCM over the last 50 years, the main protagonists, the enormous accomplishments they have achieved for the profession, their philosophy, their clinical perspectives and visions for the future.展开更多
基金funded by the Department of Biotechnology,Ministry of Science and Technology,Government of India(DBT)under grant number ADMaC DBT-NER/LIVS/11/2012.
文摘We report the development of a triplex nucleic acid lateral flow immunoassay(NALFIA)for the detection of the genomes of Nipah virus(NiV),Middle East respiratory syndrome coronavirus(MERS-CoV)and Reston ebolavirus(REBOV),which are intended for screening bats as well as other hosts and reservoirs of these three viruses.Our triplex NALFIA is a two-step assay format:the target nucleic acid in the sample is first amplified using tagged primers,and the tagged dsDNA amplicons are captured by antibodies immobilized on the NALFIA device,resulting in signal development from the binding of a streptavidin-colloidal gold conjugate to a biotin tag on the captured amplicons.Triplex amplification of the N gene of NiV,the UpE gene of MERS-CoV,and the Vp40 gene of REBOV was optimized,and three compatible combinations of hapten labels and antibodies were identified for end point detection.The lowest RNA copy numbers detected by the triplex NALFIA were 8.21e4 for the NiV N target,7.09e1 for the MERS-CoV UpE target,and 1.83e4 for the REBOV Vp40 target.Using simulated samples,the sensitivity and specificity for MERS-CoV and REBOV targets were estimated to be 100%,while the sensitivity and specificity for the NiV target were 91%and 93.3%,respectively.The compliance rate between triplex NALFIA and real-time RT‒PCR was 92%for the NiV N target and 100%for the MERS-CoV UpE and REBOV Vp40 targets.
文摘Based on TBB data from Meteorological Institute Research of Japan, study is carried out of the features of seasonal transition of Asian-Australian monsoons and Asian summer monsoon establishment,indicating that the transition begins as early as in April, followed by abrupt change in May-June; the Asian summer monsoon situation is fully established in June. The winter convective center in Sumatra moved steadily northwestward across the "land bridge" of the maritime continent and the Indo-China Peninsula as time goes from winter to summer, thus giving rise to the change in large scale circulations that is responsible for the summer monsoon establishment over SE Asia and India; the South China Sea to the western Pacific summer monsoon onset bears a close relation to the active convection in the Indo China Peninsula and steady eastward retreat of the subtropical TBB high-value band,corresponding to the western Pacific subtropical high.
文摘This article details a public acupuncture demonstration that took place on May 9,1972,in San Francisco,California.It was conducted by two traditional Chinese medicine doctors Pien Bae Chi(卞伯岐)and Leung Kok Yuen(梁觉玄),and was observed by 500 American medical doctors.This was an important public acupuncture demonstration in the early history of acupunccture in the United States(US).It directly promoted the passage of the first acupuncture bill by California government and had a significant impact on the legalization of acupuncture in California.
文摘It has historically been very difficult to trace the history of the westward transmission of Chinese medicine through the accounts of its protagonists. Many of the early scholars such as Jack Worsley, Dick Van Buren, and Joe Goodman were reluctant to divulge information about the source of their knowledge, or their professional qualifications. Others, such as John Shen and Hong Yuan-bain were early 20th century immigrants who transmitted highly personalized versions of acupuncture and Chinese medicine to select disciples. Eventually, a new class of scholars appeared, including names such as Ted Kaptchuk, Peter Deadman, Nigel Wiseman, William Morris, Peter Eckman, John Mc Donald, Charles Buck, and the late Giovanni Maciocia who looked for answers back in China, developed translation methodologies and terminology, compiled the main textbooks currently in use at TCM colleges, overcame enormous scholastic adversity, developed courses and pursued the regulation and accreditation of TCM in various countries. This special issue synopsizes the path towards the global acculturation of TCM over the last 50 years, the main protagonists, the enormous accomplishments they have achieved for the profession, their philosophy, their clinical perspectives and visions for the future.
