[ Objective] This paper aimed to understand drug resistance of Escherichia coli isolated from pets in Guangzhou. I Method ] Broth microdilution method was used to determine the minimal inhibitory concentration （MIC）...[ Objective] This paper aimed to understand drug resistance of Escherichia coli isolated from pets in Guangzhou. I Method ] Broth microdilution method was used to determine the minimal inhibitory concentration （MIC） of ceftiofur, enrofloxacin and other 11 kinds of antibiotics for antimicrobial agent susceptibility testing of 120 strains of clinical E. coli and the experimental data were processed by WHONET 5.4 software. Based on the results of drug resistance pattern analysis, it helped to analyze and study the resistance mechanism. EResultl Clinical E. coli isolated from pets showed a different drug resistance to the 13 kinds of veterinary clinical antibiotics, and the different sources of E. coli also showed a different drug resistance pattern.[Cenclmion] The study provided a theoretical basis for the clinical use of drugs and druq resistance surveillance.展开更多
Introduction:This study aimed to characterize the genetic diversity and antimicrobial resistance patterns of Campylobacter isolates collected throughout China from 2020 to 2023.Methods:Campylobacter isolates analyzed ...Introduction:This study aimed to characterize the genetic diversity and antimicrobial resistance patterns of Campylobacter isolates collected throughout China from 2020 to 2023.Methods:Campylobacter isolates analyzed in this study were obtained from the National Pathogen Identification Network Center database,maintained by the National Institute for Infectious Disease Control and Prevention of the Chinese Center for Disease Control and Prevention.Antimicrobial susceptibility testing(AST)was performedagainsteleven antimicrobial agents.Genomic characteristics were analyzed through comprehensive genome sequence analysis.Results:Between 2020 and 2023,the National Pathogen Identification Network documented 1,077 Campylobacter jejuni(C.jejuni)and 221 Campylobacter coli(C.coli)isolates.Most isolates originated from patients presenting with diarrhea.Antimicrobial susceptibility testing was conducted on 634 C.jejuni and165C.coli isolates.The tested isolates demonstrated high resistance rates to nalidixic acid(78.22%),ciprofloxacin(78.07%),and tetracycline(71.96%).Longitudinal analysis of antimicrobial susceptibility testing results revealed a declining resistance trend from 2020 to 2023.Whole genome sequences were obtained for 540 C.jejuni and 125 C.coli isolates within the database.Virulence factors and antibiotic resistance determinants were identified using the VFDB and CARD databases,respectively.Phylogenetic relationships were established through Snippy 4.0 software analysis based on core genome comparisons.Conclusions:This comprehensive analysis.describes the antibiotic resistance profiles and genetic characteristics of Campylobacter isolates collected through the Identification Network Database from 2020 to 2023,establishing a foundational framework for campylobacteriosis control and prevention strategies in China.展开更多
sing the natural limestone samples taken from the field with dimension of 500 mm×500 mm×1 000 mm, the D-D (dilatancy-diffusion) seismogeny pattern was modeled under the condition of water injection, which ob...sing the natural limestone samples taken from the field with dimension of 500 mm×500 mm×1 000 mm, the D-D (dilatancy-diffusion) seismogeny pattern was modeled under the condition of water injection, which observes the time-space evolutionary features about the relative physics fields of the loaded samples from deformation, formation of microcracks to the occurrence of main rupture. The results of observed apparent resistivity show: ① The process of the deformation from microcrack to main rupture on the loaded rock sample could be characterized by the precursory spatial-temporal changes in the observation of apparent resistivity; ② The precursory temporal changes of observation in apparent resistivity could be divided into several stages, and its spatial distribution shows the difference in different parts of the rock sample; ③ Before the main rupture of the rock sample the obvious ″tendency anomaly′ and ′short-term anomaly″ were observed, and some of them could be likely considered as the ″impending earthquake ″anomaly precursor of apparent resistivity. The changes and distribution features of apparent resistivity show that they are intrinsically related to the dilatancy phenomenon of the loaded rock sample. Finally, this paper discusses the mechanism of resistivity change of loaded rock sample theoretically.展开更多
In this paper, an electrical resistance tomography(ERT) imaging method is used as a classifier, and then the Dempster-Shafer's evidence theory with fuzzy clustering is integrated to improve the ERT image quality. ...In this paper, an electrical resistance tomography(ERT) imaging method is used as a classifier, and then the Dempster-Shafer's evidence theory with fuzzy clustering is integrated to improve the ERT image quality. The fuzzy clustering is applied to determining the key mass function, and dealing with the uncertain, incomplete and inconsistent measured imaging data in ERT. The proposed method was applied to images with the same investigated object under eight typical current drive patterns. Experiments were performed on a group of simulations using COMSOL Multiphysics tool and measurements with a piece of porcine lung and a pair of porcine kidneys as test materials. Compared with any single drive pattern, the proposed method can provide images with a spatial resolution of about 10% higher, while the time resolution was almost the same.展开更多
Objective:To assess multidrug resistant(MDR)Escherichia coli(E.coli)isolates from patients with urinary tract infection.Methods:From February to June 2014,a cross sectional study was conducted among urinary tract infe...Objective:To assess multidrug resistant(MDR)Escherichia coli(E.coli)isolates from patients with urinary tract infection.Methods:From February to June 2014,a cross sectional study was conducted among urinary tract infection patients at the University of Gondar Hospital.Culture and disk diffusion method were used for E.coli isolation and to determine the antibiotic susceptibility patterns.Data were entered and analyzed using SPSS version 20.P<0.05 was considered as statistically significant.Results:A total of 112 E.coli isolates were identified and the rate of isolation was higher among female participants(28.7%;P=0.03).Of the isolates,104(92.9%)were MDR E.coli;and the isolates showed high resistance rates towards ampicillin(99%),cotrimoxazole(69%),chloramphenicol(58.7%),gentamycin(56.7%)and ceftazidime(55.8%).However,comparative isolates showed low resistance rates to ciprofloxacin(1%),cefepime(8.7%),and ceftriaxone(11.5%).Moreover,resistance rates of MDR E.coli isolates were significantly higher than non-MDR strains for ceftazidime(55.8%versus 12.5%;P=0.015),and ampicillin(99%versus 87.5%;P=0.018).Conclusions:High prevalence of MDR E.coli isolates was observed in this study.Regular monitoring of antibiotic resistance rates is necessarily required to improve and revise empirical antibiotic therapy protocols.展开更多
基金supported by the Special Fund Project for the Institute Science and Technology Development and Demostration Extention of Henan Provincial Academy of Argricultural Sciences(Research of Zoonotic Small Animal Diseases)the Project for the 2012 Annual"University Students'Innovation Training Program",Southwest University for Nationalities(201210656017)the Special Fund Project for the Central University Basic Scientific Research Fund,Southwest University for Nationalities(11NZYTD02)
文摘[ Objective] This paper aimed to understand drug resistance of Escherichia coli isolated from pets in Guangzhou. I Method ] Broth microdilution method was used to determine the minimal inhibitory concentration （MIC） of ceftiofur, enrofloxacin and other 11 kinds of antibiotics for antimicrobial agent susceptibility testing of 120 strains of clinical E. coli and the experimental data were processed by WHONET 5.4 software. Based on the results of drug resistance pattern analysis, it helped to analyze and study the resistance mechanism. EResultl Clinical E. coli isolated from pets showed a different drug resistance to the 13 kinds of veterinary clinical antibiotics, and the different sources of E. coli also showed a different drug resistance pattern.[Cenclmion] The study provided a theoretical basis for the clinical use of drugs and druq resistance surveillance.
基金Supported by the National Key Research and Development Program of China(Grant Number 2021YFC2301000)the Capital's Funds for Health Improvement and Research(No.2024-2G-7106).
文摘Introduction:This study aimed to characterize the genetic diversity and antimicrobial resistance patterns of Campylobacter isolates collected throughout China from 2020 to 2023.Methods:Campylobacter isolates analyzed in this study were obtained from the National Pathogen Identification Network Center database,maintained by the National Institute for Infectious Disease Control and Prevention of the Chinese Center for Disease Control and Prevention.Antimicrobial susceptibility testing(AST)was performedagainsteleven antimicrobial agents.Genomic characteristics were analyzed through comprehensive genome sequence analysis.Results:Between 2020 and 2023,the National Pathogen Identification Network documented 1,077 Campylobacter jejuni(C.jejuni)and 221 Campylobacter coli(C.coli)isolates.Most isolates originated from patients presenting with diarrhea.Antimicrobial susceptibility testing was conducted on 634 C.jejuni and165C.coli isolates.The tested isolates demonstrated high resistance rates to nalidixic acid(78.22%),ciprofloxacin(78.07%),and tetracycline(71.96%).Longitudinal analysis of antimicrobial susceptibility testing results revealed a declining resistance trend from 2020 to 2023.Whole genome sequences were obtained for 540 C.jejuni and 125 C.coli isolates within the database.Virulence factors and antibiotic resistance determinants were identified using the VFDB and CARD databases,respectively.Phylogenetic relationships were established through Snippy 4.0 software analysis based on core genome comparisons.Conclusions:This comprehensive analysis.describes the antibiotic resistance profiles and genetic characteristics of Campylobacter isolates collected through the Identification Network Database from 2020 to 2023,establishing a foundational framework for campylobacteriosis control and prevention strategies in China.
文摘sing the natural limestone samples taken from the field with dimension of 500 mm×500 mm×1 000 mm, the D-D (dilatancy-diffusion) seismogeny pattern was modeled under the condition of water injection, which observes the time-space evolutionary features about the relative physics fields of the loaded samples from deformation, formation of microcracks to the occurrence of main rupture. The results of observed apparent resistivity show: ① The process of the deformation from microcrack to main rupture on the loaded rock sample could be characterized by the precursory spatial-temporal changes in the observation of apparent resistivity; ② The precursory temporal changes of observation in apparent resistivity could be divided into several stages, and its spatial distribution shows the difference in different parts of the rock sample; ③ Before the main rupture of the rock sample the obvious ″tendency anomaly′ and ′short-term anomaly″ were observed, and some of them could be likely considered as the ″impending earthquake ″anomaly precursor of apparent resistivity. The changes and distribution features of apparent resistivity show that they are intrinsically related to the dilatancy phenomenon of the loaded rock sample. Finally, this paper discusses the mechanism of resistivity change of loaded rock sample theoretically.
基金Supported by National Natural Science Foundation of China(No.61774014 and No.60772080)
文摘In this paper, an electrical resistance tomography(ERT) imaging method is used as a classifier, and then the Dempster-Shafer's evidence theory with fuzzy clustering is integrated to improve the ERT image quality. The fuzzy clustering is applied to determining the key mass function, and dealing with the uncertain, incomplete and inconsistent measured imaging data in ERT. The proposed method was applied to images with the same investigated object under eight typical current drive patterns. Experiments were performed on a group of simulations using COMSOL Multiphysics tool and measurements with a piece of porcine lung and a pair of porcine kidneys as test materials. Compared with any single drive pattern, the proposed method can provide images with a spatial resolution of about 10% higher, while the time resolution was almost the same.
文摘Objective:To assess multidrug resistant(MDR)Escherichia coli(E.coli)isolates from patients with urinary tract infection.Methods:From February to June 2014,a cross sectional study was conducted among urinary tract infection patients at the University of Gondar Hospital.Culture and disk diffusion method were used for E.coli isolation and to determine the antibiotic susceptibility patterns.Data were entered and analyzed using SPSS version 20.P<0.05 was considered as statistically significant.Results:A total of 112 E.coli isolates were identified and the rate of isolation was higher among female participants(28.7%;P=0.03).Of the isolates,104(92.9%)were MDR E.coli;and the isolates showed high resistance rates towards ampicillin(99%),cotrimoxazole(69%),chloramphenicol(58.7%),gentamycin(56.7%)and ceftazidime(55.8%).However,comparative isolates showed low resistance rates to ciprofloxacin(1%),cefepime(8.7%),and ceftriaxone(11.5%).Moreover,resistance rates of MDR E.coli isolates were significantly higher than non-MDR strains for ceftazidime(55.8%versus 12.5%;P=0.015),and ampicillin(99%versus 87.5%;P=0.018).Conclusions:High prevalence of MDR E.coli isolates was observed in this study.Regular monitoring of antibiotic resistance rates is necessarily required to improve and revise empirical antibiotic therapy protocols.
