Nucleolus is a membrane-less organelle where ribosomes are assembled, and ribosomal RNAs(r RNAs)transcribed and processed. The assembled ribosomes composed of ribosomal proteins and r RNAs synthesize proteins for cell...Nucleolus is a membrane-less organelle where ribosomes are assembled, and ribosomal RNAs(r RNAs)transcribed and processed. The assembled ribosomes composed of ribosomal proteins and r RNAs synthesize proteins for cell survival. In plants, the loss of nucleolar ribosomal proteins often causes gametophytically or embryonically lethality. The amount of r RNAs are under stringent regulation according to demand and partially switched off by epigenetic modifications. However, the molecular mechanism for the selective activation or silencing is still unclear, and the transcriptional coordination of r RNAs and ribosomal proteins is also unknown. Here, we report the critical role of three Arabidopsis nucleolar proteins HDT1,HDT2, and HDT3 in fertility and transcription of r DNAs and r RNA processing-related genes through histone acetylation. This study highlights the important roles of transcriptional repression of ribosome biogenesisrelated genes for plant reproductive development.展开更多
Turbots( Schophthalmus maximus), one of the most important economic marine flatfish species, fail to undergo final spawning and spermiation naturally under artificial farming conditions. In vertebrates, reproduction i...Turbots( Schophthalmus maximus), one of the most important economic marine flatfish species, fail to undergo final spawning and spermiation naturally under artificial farming conditions. In vertebrates, reproduction is regulated by the brain-pituitary-gonadal axis(BPG-axis), and gonadotropin releasing hormone(GnRH) is one of its key components. Therefore, to better understand the physiology of reproduction in the turbot, three of the genes encoding GnRH subtypes— sbGnRH, c GnRH-II and sGnRH —were cloned and sequenced by isolating the cDNA sequences. The localizations and patterns of expression of their mRNAs were also evaluated during seasonal gonadal development. All three mRNAs were expressed abundantly in the brain; sbGnRH and sGnRH mRNAs were also detected in the gonads and pituitary gland, and sbGnRH expression was much higher than that of sGnRH, indicating the critical role of sbGnRH in regulating the BPG-axis. Moreover, the brain expression patterns of sbGnRH and sGnRH mRNAs showed an increased trend during gonadal development, peaking in mature stages. This indicated the direct regulation of gonadal development by the GnRH system. In addition, c GnRH-II mRNA expression showed no significant variations, suggesting that c GnRH-II is not critically involved in the control of reproduction. Further, the mRNA abundances of the three GnRH forms in the breeding season were significantly higher than those in immature and post-breeding stages in all analyzed brain areas. Therefore, we propose that sbGnRH is the most important hormone for the regulation of reproduction in turbot via the BPG-axis. These results will help in better understanding the reproductive endocrine mechanisms of turbots and lay the groundwork for additional studies aimed at comparing the reproductive physiology of wild individuals with those raised under artificial conditions.展开更多
RNA N6-methyladenine(m^(6)A)modification represents a pivotal epigenetic modification that facilitates the remodeling of gene expression and regulates a variety of biological processes via certain post-transcriptional...RNA N6-methyladenine(m^(6)A)modification represents a pivotal epigenetic modification that facilitates the remodeling of gene expression and regulates a variety of biological processes via certain post-transcriptional mechanisms.However,the specific function of RNA m^(6)A modification in insect male reproduction remains unclear.In this study,we explored the molecular mechanism by which METTL3/METTL14-mediated RNA m^(6)A modification regulates male reproduction in the invasive pest Bactrocera dorsalis.The results showed that BdMettl3 and BdMettl14 were highly expressed in fat body(FB)and male accessory glands(MAGs).Knockout of BdMettl3 or BdMettl14 decreased the expression level of m^(6)A in B.dorsalis,resulting in testicular deformities and a significant reduction of viable sperm number.Specifically,BdMettl3 or BdMettl14 knockout reduced the titer of 20-hydroxyecdysone(20E,the active form of ecdysone)in males.The messenger RNA(mRNA)of Disembodied,one of the 20E synthesis genes,was modified by m^(6)A,and its expression increased the titer of 20E.The mRNA m^(6)A level of Disembodied obviously decreased after the knockout of BdMettl3 or BdMettl14,suggesting that RNA m^(6)A modification regulates testis development and fecundity by modulating 20E synthesis.Taken together,this study indicates that METTL3/METTL14-mediated RNA m^(6)A modification presents a new regulatory mechanism for male reproduction in B.dorsalis,serving as a potential target for the control of B.dorsalis.展开更多
Fatty acids and their derivatives are essential building blocks for anther cuticle and pollen wall formation.Disruption of lipid metabolism during anther and pollen development often leads to genic male sterility(GMS)...Fatty acids and their derivatives are essential building blocks for anther cuticle and pollen wall formation.Disruption of lipid metabolism during anther and pollen development often leads to genic male sterility(GMS).To date,many lipid metabolism-related GMS genes that are involved in the formation of anther cuticle,pollen wall,and subcellular organelle membranes in anther wall layers have been identified and characterized.In this review,we summarize recent progress on characterizing lipid metabolism-related genes and their roles in male fertility and other aspects of reproductive development in plants.On the basis of cloned GMS genes controlling biosynthesis and transport of anther cutin,wax,sporopollenin,and tryphine\r\Arabidopsis,rice,and maize as well as other plant species,updated lipid metabolic networks underlying anther cuticle development and pollen wall formation were proposed.Through bioinformatics analysis of anther RNA-sequencing datasets from three maize inbred lines(Oh43,W23,and B73),a total of 125 novel lipid metabolism-related genes putatively involved in male fertility in maize were deduced.More,we discuss the pathways regulating lipid metabolism-related GMS genes at the transcriptional and post-transcriptional levels.Finally,we highlight recent findings on lipid metabolism-related genes and their roles in other aspects of plant reproductive development.A comprehensive understanding of lipid metabolism,genes involved,and their roles in plant reproductive development will facilitate the application of lipid metabolism-related genes in gene editing,haploid and callus induction,molecular breeding and hybrid seed production in crops.展开更多
EMBRYONIC FLOWER （EMF） genes are required to maintain vegetative development via repression of flower homeotic genes in Arabidopsis. Removal of EMF gene function caused plants to flower upon germination, producing a...EMBRYONIC FLOWER （EMF） genes are required to maintain vegetative development via repression of flower homeotic genes in Arabidopsis. Removal of EMF gene function caused plants to flower upon germination, producing abnormal and sterile flowers. The pleiotropic effect of ernfl mutation suggests its requirement for gene programs involved in diverse developmental processes. Transgenic plants harboring EMF1 promoter：：glucuronidase （GUS） reporter gene were generated to investigate the temporal and spatial expression pattern of EMF1. These plants displayed differential GUS activity in vegetative and flower tissues, consistent with the role of EMF1 in regulating multiple gene programs. EMFI：：GUS expression pattern in emf mutants suggests organ-specific auto-regulation. Sense- and antisense （as） EMF1 cDNA were expressed under the control of stage- and tissue-specific promoters in transgenic plants. Characterization of these transgenic plants showed that EMF1 activity is required in meristematic as well as differentiating tissues to rescue emf mutant phenotype. Temporal removal or reduction of EMF1 activity in the embryo or shoot apex of wild-type seedlings was sufficient to cause early flowering and terminal flower formation in adult plants. Such reproductive cell memory is reflected in the flower MADS-box gene activity expressed prior to flowering in these early flowering plants. However, temporal removal of EMF1 activity in flower meristem did not affect flower development. Our results are consistent with EMF1＇s primary role in repressing flowering in order to allow for vegetative growth.展开更多
The reproductive success of flowering plants,which directly affects crop yield,is sensitive to environmental changes.A thorough understanding of how crop reproductive development adapts to climate changes is vital for...The reproductive success of flowering plants,which directly affects crop yield,is sensitive to environmental changes.A thorough understanding of how crop reproductive development adapts to climate changes is vital for ensuring global food security.In addition to being a high-value vegetable crop,tomato is also a model plant used for research on plant reproductive development.Tomato crops are cultivated under highly diverse climatic conditions worldwide.Targeted crosses of hybrid varieties have resulted in increased yields and abiotic stress resistance;however,tomato reproduction,especially male reproductive development,is sensitive to temperature fluctuations,which can lead to aborted male gametophytes,with detrimental effects on fruit set.We herein review the cytological features as well as genetic and molecular pathways influencing tomato male reproductive organ development and responses to abiotic stress.We also compare the shared features among the associated regulatory mechanisms of tomato and other plants.Collectively,this review highlights the opportunities and challenges related to characterizing and exploiting genic male sterility in tomato hybrid breeding programs.展开更多
Reproduction and development are complex couple-dependent processes. Risk assessment for these health outcomes requires the use of biomarkers to link exposures to disease. Biological markers of susceptability, externa...Reproduction and development are complex couple-dependent processes. Risk assessment for these health outcomes requires the use of biomarkers to link exposures to disease. Biological markers of susceptability, external dose, internal dose, biologically effective dose, early or late biological responses, altered reproductive or developmental function, and reproductive or developmental disease are introduced. Using these biomarkers it is possible to define a biologically based risk assessment methodology for reproductive and developmental toxicity. Risk assessment for reproductive toxicity requires definition of male and female fecundity, couple-specific factors, spontaneous abortion, rate, and other factors. Using using sperm count as a biomarker for male fecundity, an example of a reproductive risk assessment using biomarkers is performed.展开更多
Developmental and reproductive toxicity(DART)endpoint entails a toxicological assessment of all developmental stages and reproductive cycles of an organism.In silico tools to predict DART will provide a method to asse...Developmental and reproductive toxicity(DART)endpoint entails a toxicological assessment of all developmental stages and reproductive cycles of an organism.In silico tools to predict DART will provide a method to assess this complex toxicity endpoint and will be valuable for screening emerging pollutants as well as for m anaging new chemicals in China.Currently,there are few published DART prediction models in China,but many related research and development projects are in progress.In 2013,WU et al.published an expert rule-based DART decision tree(DT).This DT relies on known chemical structures linked to DART to forecast DART potential of a given chemical.Within this procedure,an accurate DART data interpretation is the foundation of building and expanding the DT.This paper excerpted case studies demonstrating DART data curation and interpretation of four chemicals(including 8-hydroxyquinoline,3,5,6-trichloro-2-pyridinol,thiacloprid,and imidacloprid)to expand the existing DART DT.Chemicals were first selected from the database of Solid Waste and Chemicals Management Center,Ministry of Ecology and Environment(MEESCC)in China.The structures of these 4 chemicals were analyzed and preliminarily grouped by chemists based on core structural features,functional groups,receptor binding property,metabolism,and possible mode of actions.Then,the DART conclusion was derived by collecting chemical information,searching,integrating,and interpreting DART data by the toxicologists.Finally,these chemicals were classified into either an existing category or a new category via integrating their chemical features,DART conclusions,and biological properties.The results showed that 8-hydroxyquinoline impacted estrous cyclicity,s exual organ weights,and embryonal development,and 3,5,6-trichloro-2-pyridinol caused central nervous system(CNS)malformations,which were added to an existing subcategory 8e(aromatic compounds with multi-halogen and nitro groups)of the DT.Thiacloprid caused dystocia and fetal skeletal malformation,and imidacloprid disrupted the endocrine system and male fertility.They both contain 2-chloro-5-methylpyridine substituted imidazolidine c yclic ring,which were expected to create a new category of neonicotinoids.The current work delineates a t ransparent process of curating toxicological data for the purpose of DART data interpretation.In the presence of sufficient related structures and DART data,the DT can be expanded by iteratively adding chemicals within the a pplicable domain of each category or subcategory.This DT can potentially serve as a tool for screening emerging pollutants and assessing new chemicals in China.展开更多
Tea plant(Camellia sinensis(L.)O.Kuntze)is a cold-sensitive leaf-harvesting crop whose growth,yield,and processed tea quality are all inhibited by low temperatures.Therefore,identifying the regulatory genes involved i...Tea plant(Camellia sinensis(L.)O.Kuntze)is a cold-sensitive leaf-harvesting crop whose growth,yield,and processed tea quality are all inhibited by low temperatures.Therefore,identifying the regulatory genes involved in tea plant growth and freezing tolerance is crucial for genetic improvement.WRKY transcription factors regulate various plant processes,including growth and development,stress responses,and metabolite biosynthesis.However,the molecular network through which WRKY coordinates these pathways in tea plants remains unclear.In this study,we revealed that CsWRKY57L,a cold-inducible WRKY IIc subfamily member,positively regulated freezing tolerance by directly promoting flavonoid accumulation in tea plants.Transient suppression of CsWRKY57L weakened the freezing tolerance of tea plants by reducing flavonoid content and suppressing the C-repeat-binding factor(CBF)-cold-responsive(COR)gene pathway.In contrast,heterologous overexpression of CsWRKY57L in Arabidopsis had the opposite effect.Additionally,overexpression of CsWRKY57L inhibited reproductive development and accelerated senescence in Arabidopsis.Interaction analysis revealed that CsWRKY57L directly binds to the promoters of CsSWEET1a,CsSWEET15,and AtSWEET15,which encode sugar transporters essential for plant reproductive development,and inhibits their transcription.Overall,the study revealed a dual role of CsWRKY57L in promoting freezing tolerance via flavonoid biosynthesis and inhibiting reproductive development by regulating SWEETs expression.This study uncovers a novel mechanism whereby CsWRKY57L coordinately regulates both stress responses and growth in tea plants,providing a molecular basis for breeding low-temperature-tolerant varieties with restricted reproductive development.展开更多
The process of double fertilization and the characters of embryo and endosperm development in an autotetraploid polyembryonic mutant rice IR36-Shuang were studied with a laser scanning confocal microscopy. Some abnorm...The process of double fertilization and the characters of embryo and endosperm development in an autotetraploid polyembryonic mutant rice IR36-Shuang were studied with a laser scanning confocal microscopy. Some abnormalities including degenerated ovary, abortive embryo sac, single fertilization, double-ovule and double-embryo and so on. were found dudng double fertilization and embryo development in IR36-Shuang. The rate of the abnormalities was 46.67% in IR36-Shuang, significantly higher than that in the control, an autotetraploid rice line IR36-4X （33.00%）. Cytological and embryonic evidences were provided for seed setting decline and the initiation of additional embryo in IR36-Shuang.展开更多
Since estrogenic pollutants and phytoestrogens can cause the disorder of the reproductive system, the effects of a soybean milk product（Vegemil^（？） containing 162 ppm isoflavones） on the feto-neonatal development...Since estrogenic pollutants and phytoestrogens can cause the disorder of the reproductive system, the effects of a soybean milk product（Vegemil^（？） containing 162 ppm isoflavones） on the feto-neonatal development, including male reproductive function, were investigated. Pregnant rats were fed the soybean milk（5% or 100% in drinking water）from gestational day（GD） 6 to parturition or to post-natal day（PND） 56. Specifically, the rats were divided into 4 groups： the control group（drinking water）, the GD5% group（5% soybean milk during only the GD period）, the GDPND5% group（5% soybean milk during the GD and PND periods）, and the GD-PND100% group（100% soybean milk instead of water during the GD and PND periods）. During the gestational, lactational, and developmental periods, the reproductive and developmental parameters of dams and offspring were observed. Feeding soybean milk did not affect the birth and physical development of both male and female offspring. At PND57, the weights of the testes and epididymides of F1 males significantly increased by feeding a high concentration of the soybean milk（GDPND100%）. In addition, feeding of the soybean milk during both the GD and PND periods（GD-PND5% and GDPND100%） enhanced the sperm counts and motility. The results indicate that soybean milk is safe for embryos,fetuses, and offspring, and improves the post-generational development of male reproductive function.展开更多
With a history of 96 years,the Institute of Zoology(IOZ)is one of the oldest scientific research institutions of the Chinese Academy of Sciences(CAS).Since its establishment,IOZ has consistently served the national st...With a history of 96 years,the Institute of Zoology(IOZ)is one of the oldest scientific research institutions of the Chinese Academy of Sciences(CAS).Since its establishment,IOZ has consistently served the national strategy and pursued the forefront of science.Since 1978 when China implemented a reform and opening up policy,especially over the past two decades,IOZ has achieved a series of world-leading science and technology achievements,deepening our understanding of mechanisms of cell programming and reprogramming,reproductive and developmental regulation,stem cell and regenerative medicine,integrated management of pest insects and rodents,endangered mechanisms and conservation of species,and animal taxonomy and evolution.In the future,IOZ will strive to seize the science highland by optimizing the layout of its disciplinary development,attracting and fostering talents,and constructing world-class research facilities and platforms,aiming to be a leading institution for the continuous innovation and development of life science both in China and the world.展开更多
In most fish,reproduction is seasonal or periodic under the suitable conditions.In turbot(Scophthalmus maximus)farms,one of the most economically important marine flatfish species,changes in daylength could cause chan...In most fish,reproduction is seasonal or periodic under the suitable conditions.In turbot(Scophthalmus maximus)farms,one of the most economically important marine flatfish species,changes in daylength could cause changes in the spawning time.In this study,to characterize the regulation of reproductive physiology following light signals,three melatonin receptors(Mtnr)investigated in turbot were named sm Mtnr1,sm Mtnr2,and sm Mtnr1 c.Distinct expression profiles demonstrated that Mtnr m RNAs were concentrated in the brain(as detected in the hypothalamus(Hy)and mesencephalon(Me)),gonad and eye.The most abundant Mtnr1 and Mtnr2 m RNA expression levels were detected in the central nervous system at the beginning of the breeding season,suggesting that Mtnr1 and Mtnr2 may play vital roles in the regulation of turbot gonadal development.In addition,the melatonin profiles gradually increased and reached to the highest level at the spawning stage,indicating that melatonin is a potent hormone in the regulation of fish oocyte growth and maturation.The results of this study suggested that melatonin is the primary factor that transduces the light signal and regulates the physiological functions of turbot seasonal reproduction.Moreover,the results of this study may establish a foundation for further research seeking to identify fish melatonin receptors involved in the gonadal development and gamete maturation.展开更多
Dynamic DNA methylation represses transposable elements(TEs)and regulates gene activity,playing a pivotal role in plant development.Although substantial progress has been made in understanding DNA methylation reprogra...Dynamic DNA methylation represses transposable elements(TEs)and regulates gene activity,playing a pivotal role in plant development.Although substantial progress has been made in understanding DNA methylation reprogramming during germline development in Arabidopsis thaliana,whether similar mechanisms exist in other dicot plants remains unclear.Here,we analyzed DNA methylation levels in meiocytes,microspores,and pollens of Brassica Rapa using whole-genome bisulfite sequencing(WGBS).Global DNA methylation analysis revealed similar CHH methylation reprogramming compared to Arabidopsis,while distinct patterns were observed in the dynamics of global CG and CHG methylation in B.rapa.Differentially methylated region(DMR)analysis identified specifically methylated loci in the male sex cells of B.Rapa with a stronger tendency to target genes,similar to observations in Arabidopsis.Additionally,we found that the activity and genomic targeting preference of the small RNA-directed DNA methylation(RdDM)were altered during B.Rapa male germline development.A subset of long terminal repeat(LTR)TEs were activated,possibly due to the dynamic regulation of DNA methylation during male sexual development in B.Rapa.These findings provided new insights into the evolution of epigenetic reprogramming mechanisms in plants.展开更多
Organic contaminants can be detected at low concentrations in drinking water, raising concerns for human health, particularly in reproduction. In this respect, we attempted to use the zebrafish as a bioindicator to de...Organic contaminants can be detected at low concentrations in drinking water, raising concerns for human health, particularly in reproduction. In this respect, we attempted to use the zebrafish as a bioindicator to detect the possible presence of these substances in drinking water, aiming to define the most relevant parameters to detect these substances, which particularly affect the development and reproduction of zebrafish. To this end, batches of30 embryos with the chorion intact were cultured in drinking waters from different sources,throughout their full life-cycle up to 5 months, in 20 L tanks. Six replicates were performed in all water groups, with a total of 24 aquariums. Two generations（F0 and F1） were studied and the following parameters were tested： in the F0 generation, survival and abnormality rates evaluated at 5 dpf（days post-fertilization） and at 5 mpf（months post-fertilization）,the onset of spawning and the fertility rate from 3 mpf to 5 mpf, and the sex ratio and underdeveloped specimens at 5 mpf. Furthermore, in the F0 offspring（F1）, survival and abnormality rates were evaluated at 5 dpf and the hatching rate at 72 hpf. These results revealed that the hatching rate is the most sensitive parameter to distinguish different levels of effects between waters during the early life stages, whereas the rate of underdeveloped specimens is more suitable at later life stages. Regarding adult reproduction, fertility rate was the most sensitive parameter. The possible reversibility or accumulative nature of such effects will be studied in future work.展开更多
A novel gene, GhSERK1, was identified in cotton. It encoded a protein belonging to the somatic embryogenesis receptor- like kinase （SERK） family. The genomic sequence of GhSERK1 was 6 920 bp in length, containing a ...A novel gene, GhSERK1, was identified in cotton. It encoded a protein belonging to the somatic embryogenesis receptor- like kinase （SERK） family. The genomic sequence of GhSERK1 was 6 920 bp in length, containing a predicted transcriptional start site （TSS）. Its full-length cDNA was 2 502 bp, encoding a protein of 627 amino acids. Sequence analysis of GhSERK1 revealed high levels of similarity to other reported SERKs, as well as a conserved intron/exon structure that was unique to members of the SERK family. Expression analysis showed that GhSERK1 mRNA was present in all organs of cotton plants and at different developmental stages, but its transcripts were most abundant in reproductive organs. Compared with that of the male-fertile line, the level of GhSERK1 mRNA was lower in the anther of the male-sterile cotton line, in which the pollen development was defected. Taken together, these findings illustrated that the GhSERK1 play a critical role during the anther formation, and may also have a broad role in other aspects of plant development.展开更多
Female reproductive endocrinology plays an important role in obstetrics and gynecology, and is a component part of perinatology, family planning and geriatrics. It is closely linked with the physiological changes, dis...Female reproductive endocrinology plays an important role in obstetrics and gynecology, and is a component part of perinatology, family planning and geriatrics. It is closely linked with the physiological changes, disease conditions and health care in different reproductive stages of a woman’s life. Ovarian function consists of ovulation and hormonal secretion, which acts upon the uterine endometrium and other target organs and feeds back to the hypothalamus-pituitary unit. Thus, it is connected with other endocrine systems, cerebral cortex and the external environment. Whether the ovrian function is normal or not reflects itself clinically in the changes of menstrual cycle and reproductive status and may be evaluated by laboratory assays.展开更多
Invertase （INV） hydrolyzes sucrose into glucose and fructose, thereby playing key roles in primary metabolism and plant development. Based on their pH optima and sub-cellular locations, INVs are categorized into cel...Invertase （INV） hydrolyzes sucrose into glucose and fructose, thereby playing key roles in primary metabolism and plant development. Based on their pH optima and sub-cellular locations, INVs are categorized into cell wall, cytoplasmic, and vacuolar subgroups, abbreviated as CWlN, CIN, and VlN, respectively. The broad importance and implications of INVs in plant development and crop productivity have attracted enormous interest to examine INV function and regulation from multiple perspectives. Here, we review some exciting advances in this area over the last two decades, focusing on （1） new or emerging roles of INV in plant development and regulation at the post-translational level through interaction with inhibitors, （2） cross-talk between INV-mediated sugar signaling and hormonal control of development, and （3） sugar- and INV-mediated responses to drought and heat stresses and their impact on seed and fruit set. Finally, we discuss major questions arising from this new progress and outline future directions for unraveling mechanisms underlying INV-mediated plant development and their potential applications in plant biotechnology and agriculture.展开更多
Extreme temperatures have become an unavoidable reality across vast regions of the world.Heat waves can cause severe crop damage,intensify drought conditions,and severely impact agriculture,disrupting food systems and...Extreme temperatures have become an unavoidable reality across vast regions of the world.Heat waves can cause severe crop damage,intensify drought conditions,and severely impact agriculture,disrupting food systems and exacerbating food insecurity(Zsögön et al.,2022).Heat stress adversely affects reproductive development in plants,leading to grain or ovary abortion and causing significant yield losses(Li et al.,2012).展开更多
MY ancestors have lived in Lhasa, Tibet, for generations. I have personally witnessed the great changes in modern Tibetan society. The total population of the region has doubled since 1951. The Fourth National Census ...MY ancestors have lived in Lhasa, Tibet, for generations. I have personally witnessed the great changes in modern Tibetan society. The total population of the region has doubled since 1951. The Fourth National Census in 1990 revealed that the human fertility rate in the Tibet Autonomous Region was 27.60 per thousand, the natural population growth rate 18.40 per thousand, and the total fertility rate of women at childbearing age 4.22.展开更多
基金supported by funding from the National Natural Science Foundation of China (31991203 and 31870295)。
文摘Nucleolus is a membrane-less organelle where ribosomes are assembled, and ribosomal RNAs(r RNAs)transcribed and processed. The assembled ribosomes composed of ribosomal proteins and r RNAs synthesize proteins for cell survival. In plants, the loss of nucleolar ribosomal proteins often causes gametophytically or embryonically lethality. The amount of r RNAs are under stringent regulation according to demand and partially switched off by epigenetic modifications. However, the molecular mechanism for the selective activation or silencing is still unclear, and the transcriptional coordination of r RNAs and ribosomal proteins is also unknown. Here, we report the critical role of three Arabidopsis nucleolar proteins HDT1,HDT2, and HDT3 in fertility and transcription of r DNAs and r RNA processing-related genes through histone acetylation. This study highlights the important roles of transcriptional repression of ribosome biogenesisrelated genes for plant reproductive development.
基金Supported by the National Natural Science Foundation of China(Nos.31472264,31572602)the China Agriculture Research System(No.CARS-47)+1 种基金the Scientific and Technological Innovation Project Financially supported by Qingdao National Laboratory for Marine Science and Technology(Nos.2015ASKJ02,2015ASKJ02-03-03)the Youth Innovation Promotion Association CAS and Chinese Academy of Science and Technology Service Network Planning(No.KFJ-EWSTS-060)
文摘Turbots( Schophthalmus maximus), one of the most important economic marine flatfish species, fail to undergo final spawning and spermiation naturally under artificial farming conditions. In vertebrates, reproduction is regulated by the brain-pituitary-gonadal axis(BPG-axis), and gonadotropin releasing hormone(GnRH) is one of its key components. Therefore, to better understand the physiology of reproduction in the turbot, three of the genes encoding GnRH subtypes— sbGnRH, c GnRH-II and sGnRH —were cloned and sequenced by isolating the cDNA sequences. The localizations and patterns of expression of their mRNAs were also evaluated during seasonal gonadal development. All three mRNAs were expressed abundantly in the brain; sbGnRH and sGnRH mRNAs were also detected in the gonads and pituitary gland, and sbGnRH expression was much higher than that of sGnRH, indicating the critical role of sbGnRH in regulating the BPG-axis. Moreover, the brain expression patterns of sbGnRH and sGnRH mRNAs showed an increased trend during gonadal development, peaking in mature stages. This indicated the direct regulation of gonadal development by the GnRH system. In addition, c GnRH-II mRNA expression showed no significant variations, suggesting that c GnRH-II is not critically involved in the control of reproduction. Further, the mRNA abundances of the three GnRH forms in the breeding season were significantly higher than those in immature and post-breeding stages in all analyzed brain areas. Therefore, we propose that sbGnRH is the most important hormone for the regulation of reproduction in turbot via the BPG-axis. These results will help in better understanding the reproductive endocrine mechanisms of turbots and lay the groundwork for additional studies aimed at comparing the reproductive physiology of wild individuals with those raised under artificial conditions.
基金supported by the National Natural Science Foundation of China(no.32220103009)Earmarked Fund for China Agriculture Research System(CARS-26)Hubei Hongshan Laboratory.
文摘RNA N6-methyladenine(m^(6)A)modification represents a pivotal epigenetic modification that facilitates the remodeling of gene expression and regulates a variety of biological processes via certain post-transcriptional mechanisms.However,the specific function of RNA m^(6)A modification in insect male reproduction remains unclear.In this study,we explored the molecular mechanism by which METTL3/METTL14-mediated RNA m^(6)A modification regulates male reproduction in the invasive pest Bactrocera dorsalis.The results showed that BdMettl3 and BdMettl14 were highly expressed in fat body(FB)and male accessory glands(MAGs).Knockout of BdMettl3 or BdMettl14 decreased the expression level of m^(6)A in B.dorsalis,resulting in testicular deformities and a significant reduction of viable sperm number.Specifically,BdMettl3 or BdMettl14 knockout reduced the titer of 20-hydroxyecdysone(20E,the active form of ecdysone)in males.The messenger RNA(mRNA)of Disembodied,one of the 20E synthesis genes,was modified by m^(6)A,and its expression increased the titer of 20E.The mRNA m^(6)A level of Disembodied obviously decreased after the knockout of BdMettl3 or BdMettl14,suggesting that RNA m^(6)A modification regulates testis development and fecundity by modulating 20E synthesis.Taken together,this study indicates that METTL3/METTL14-mediated RNA m^(6)A modification presents a new regulatory mechanism for male reproduction in B.dorsalis,serving as a potential target for the control of B.dorsalis.
基金This research was supported by the National Transgenic Major Program of China(2018ZX08010-06B,2018ZX08009-22B)the National Key Research and Development Program of China(2017YFD0102001,2018YFD0100806,2017YFD0101201)+2 种基金the National Natural Science Foundation of China(31971958,31771875,31871702)the Fundamental Research Funds for the Central Universities of China(06500136)the"Ten Thousand Plan"-National High Level Talents Special Support Plan(For X.W.),and the Beijing Science&Technology Plan Program(Z191100004019005).
文摘Fatty acids and their derivatives are essential building blocks for anther cuticle and pollen wall formation.Disruption of lipid metabolism during anther and pollen development often leads to genic male sterility(GMS).To date,many lipid metabolism-related GMS genes that are involved in the formation of anther cuticle,pollen wall,and subcellular organelle membranes in anther wall layers have been identified and characterized.In this review,we summarize recent progress on characterizing lipid metabolism-related genes and their roles in male fertility and other aspects of reproductive development in plants.On the basis of cloned GMS genes controlling biosynthesis and transport of anther cutin,wax,sporopollenin,and tryphine\r\Arabidopsis,rice,and maize as well as other plant species,updated lipid metabolic networks underlying anther cuticle development and pollen wall formation were proposed.Through bioinformatics analysis of anther RNA-sequencing datasets from three maize inbred lines(Oh43,W23,and B73),a total of 125 novel lipid metabolism-related genes putatively involved in male fertility in maize were deduced.More,we discuss the pathways regulating lipid metabolism-related GMS genes at the transcriptional and post-transcriptional levels.Finally,we highlight recent findings on lipid metabolism-related genes and their roles in other aspects of plant reproductive development.A comprehensive understanding of lipid metabolism,genes involved,and their roles in plant reproductive development will facilitate the application of lipid metabolism-related genes in gene editing,haploid and callus induction,molecular breeding and hybrid seed production in crops.
文摘EMBRYONIC FLOWER （EMF） genes are required to maintain vegetative development via repression of flower homeotic genes in Arabidopsis. Removal of EMF gene function caused plants to flower upon germination, producing abnormal and sterile flowers. The pleiotropic effect of ernfl mutation suggests its requirement for gene programs involved in diverse developmental processes. Transgenic plants harboring EMF1 promoter：：glucuronidase （GUS） reporter gene were generated to investigate the temporal and spatial expression pattern of EMF1. These plants displayed differential GUS activity in vegetative and flower tissues, consistent with the role of EMF1 in regulating multiple gene programs. EMFI：：GUS expression pattern in emf mutants suggests organ-specific auto-regulation. Sense- and antisense （as） EMF1 cDNA were expressed under the control of stage- and tissue-specific promoters in transgenic plants. Characterization of these transgenic plants showed that EMF1 activity is required in meristematic as well as differentiating tissues to rescue emf mutant phenotype. Temporal removal or reduction of EMF1 activity in the embryo or shoot apex of wild-type seedlings was sufficient to cause early flowering and terminal flower formation in adult plants. Such reproductive cell memory is reflected in the flower MADS-box gene activity expressed prior to flowering in these early flowering plants. However, temporal removal of EMF1 activity in flower meristem did not affect flower development. Our results are consistent with EMF1＇s primary role in repressing flowering in order to allow for vegetative growth.
基金supported by the National Key R&D Program of China(2018YFA0900603)National Natural Science Foundation of China(U1903202)+1 种基金Major Research Plan of National Natural Science Foundation of China(31991183)to C.X.China National Postdoctoral Program for Innovative Talents(BX20220336)to D.Y。
文摘The reproductive success of flowering plants,which directly affects crop yield,is sensitive to environmental changes.A thorough understanding of how crop reproductive development adapts to climate changes is vital for ensuring global food security.In addition to being a high-value vegetable crop,tomato is also a model plant used for research on plant reproductive development.Tomato crops are cultivated under highly diverse climatic conditions worldwide.Targeted crosses of hybrid varieties have resulted in increased yields and abiotic stress resistance;however,tomato reproduction,especially male reproductive development,is sensitive to temperature fluctuations,which can lead to aborted male gametophytes,with detrimental effects on fruit set.We herein review the cytological features as well as genetic and molecular pathways influencing tomato male reproductive organ development and responses to abiotic stress.We also compare the shared features among the associated regulatory mechanisms of tomato and other plants.Collectively,this review highlights the opportunities and challenges related to characterizing and exploiting genic male sterility in tomato hybrid breeding programs.
文摘Reproduction and development are complex couple-dependent processes. Risk assessment for these health outcomes requires the use of biomarkers to link exposures to disease. Biological markers of susceptability, external dose, internal dose, biologically effective dose, early or late biological responses, altered reproductive or developmental function, and reproductive or developmental disease are introduced. Using these biomarkers it is possible to define a biologically based risk assessment methodology for reproductive and developmental toxicity. Risk assessment for reproductive toxicity requires definition of male and female fecundity, couple-specific factors, spontaneous abortion, rate, and other factors. Using using sperm count as a biomarker for male fecundity, an example of a reproductive risk assessment using biomarkers is performed.
文摘Developmental and reproductive toxicity(DART)endpoint entails a toxicological assessment of all developmental stages and reproductive cycles of an organism.In silico tools to predict DART will provide a method to assess this complex toxicity endpoint and will be valuable for screening emerging pollutants as well as for m anaging new chemicals in China.Currently,there are few published DART prediction models in China,but many related research and development projects are in progress.In 2013,WU et al.published an expert rule-based DART decision tree(DT).This DT relies on known chemical structures linked to DART to forecast DART potential of a given chemical.Within this procedure,an accurate DART data interpretation is the foundation of building and expanding the DT.This paper excerpted case studies demonstrating DART data curation and interpretation of four chemicals(including 8-hydroxyquinoline,3,5,6-trichloro-2-pyridinol,thiacloprid,and imidacloprid)to expand the existing DART DT.Chemicals were first selected from the database of Solid Waste and Chemicals Management Center,Ministry of Ecology and Environment(MEESCC)in China.The structures of these 4 chemicals were analyzed and preliminarily grouped by chemists based on core structural features,functional groups,receptor binding property,metabolism,and possible mode of actions.Then,the DART conclusion was derived by collecting chemical information,searching,integrating,and interpreting DART data by the toxicologists.Finally,these chemicals were classified into either an existing category or a new category via integrating their chemical features,DART conclusions,and biological properties.The results showed that 8-hydroxyquinoline impacted estrous cyclicity,s exual organ weights,and embryonal development,and 3,5,6-trichloro-2-pyridinol caused central nervous system(CNS)malformations,which were added to an existing subcategory 8e(aromatic compounds with multi-halogen and nitro groups)of the DT.Thiacloprid caused dystocia and fetal skeletal malformation,and imidacloprid disrupted the endocrine system and male fertility.They both contain 2-chloro-5-methylpyridine substituted imidazolidine c yclic ring,which were expected to create a new category of neonicotinoids.The current work delineates a t ransparent process of curating toxicological data for the purpose of DART data interpretation.In the presence of sufficient related structures and DART data,the DT can be expanded by iteratively adding chemicals within the a pplicable domain of each category or subcategory.This DT can potentially serve as a tool for screening emerging pollutants and assessing new chemicals in China.
基金supported by the National Natural Science Foundation of China(Grant Nos.32072630,32372774,and U22A20499)the earmarked fund for CARS(Grant No.CARS-19-01A).
文摘Tea plant(Camellia sinensis(L.)O.Kuntze)is a cold-sensitive leaf-harvesting crop whose growth,yield,and processed tea quality are all inhibited by low temperatures.Therefore,identifying the regulatory genes involved in tea plant growth and freezing tolerance is crucial for genetic improvement.WRKY transcription factors regulate various plant processes,including growth and development,stress responses,and metabolite biosynthesis.However,the molecular network through which WRKY coordinates these pathways in tea plants remains unclear.In this study,we revealed that CsWRKY57L,a cold-inducible WRKY IIc subfamily member,positively regulated freezing tolerance by directly promoting flavonoid accumulation in tea plants.Transient suppression of CsWRKY57L weakened the freezing tolerance of tea plants by reducing flavonoid content and suppressing the C-repeat-binding factor(CBF)-cold-responsive(COR)gene pathway.In contrast,heterologous overexpression of CsWRKY57L in Arabidopsis had the opposite effect.Additionally,overexpression of CsWRKY57L inhibited reproductive development and accelerated senescence in Arabidopsis.Interaction analysis revealed that CsWRKY57L directly binds to the promoters of CsSWEET1a,CsSWEET15,and AtSWEET15,which encode sugar transporters essential for plant reproductive development,and inhibits their transcription.Overall,the study revealed a dual role of CsWRKY57L in promoting freezing tolerance via flavonoid biosynthesis and inhibiting reproductive development by regulating SWEETs expression.This study uncovers a novel mechanism whereby CsWRKY57L coordinately regulates both stress responses and growth in tea plants,providing a molecular basis for breeding low-temperature-tolerant varieties with restricted reproductive development.
基金supported by the National Science & Technology Pillar Program of China in the Tenth Five-Year Plan Period(Grant No.2001BA302B)the Education Department of Henan Province, China(Grant No.2008A208019)
文摘The process of double fertilization and the characters of embryo and endosperm development in an autotetraploid polyembryonic mutant rice IR36-Shuang were studied with a laser scanning confocal microscopy. Some abnormalities including degenerated ovary, abortive embryo sac, single fertilization, double-ovule and double-embryo and so on. were found dudng double fertilization and embryo development in IR36-Shuang. The rate of the abnormalities was 46.67% in IR36-Shuang, significantly higher than that in the control, an autotetraploid rice line IR36-4X （33.00%）. Cytological and embryonic evidences were provided for seed setting decline and the initiation of additional embryo in IR36-Shuang.
文摘Since estrogenic pollutants and phytoestrogens can cause the disorder of the reproductive system, the effects of a soybean milk product（Vegemil^（？） containing 162 ppm isoflavones） on the feto-neonatal development, including male reproductive function, were investigated. Pregnant rats were fed the soybean milk（5% or 100% in drinking water）from gestational day（GD） 6 to parturition or to post-natal day（PND） 56. Specifically, the rats were divided into 4 groups： the control group（drinking water）, the GD5% group（5% soybean milk during only the GD period）, the GDPND5% group（5% soybean milk during the GD and PND periods）, and the GD-PND100% group（100% soybean milk instead of water during the GD and PND periods）. During the gestational, lactational, and developmental periods, the reproductive and developmental parameters of dams and offspring were observed. Feeding soybean milk did not affect the birth and physical development of both male and female offspring. At PND57, the weights of the testes and epididymides of F1 males significantly increased by feeding a high concentration of the soybean milk（GDPND100%）. In addition, feeding of the soybean milk during both the GD and PND periods（GD-PND5% and GDPND100%） enhanced the sperm counts and motility. The results indicate that soybean milk is safe for embryos,fetuses, and offspring, and improves the post-generational development of male reproductive function.
文摘With a history of 96 years,the Institute of Zoology(IOZ)is one of the oldest scientific research institutions of the Chinese Academy of Sciences(CAS).Since its establishment,IOZ has consistently served the national strategy and pursued the forefront of science.Since 1978 when China implemented a reform and opening up policy,especially over the past two decades,IOZ has achieved a series of world-leading science and technology achievements,deepening our understanding of mechanisms of cell programming and reprogramming,reproductive and developmental regulation,stem cell and regenerative medicine,integrated management of pest insects and rodents,endangered mechanisms and conservation of species,and animal taxonomy and evolution.In the future,IOZ will strive to seize the science highland by optimizing the layout of its disciplinary development,attracting and fostering talents,and constructing world-class research facilities and platforms,aiming to be a leading institution for the continuous innovation and development of life science both in China and the world.
基金The National Natural Science Foundation of China under contract No.31802319the Key Special Project for Introduced Talents Team of Southern Marine Science and Engineering Guangdong Laboratory(Guangzhou)under contract No.GML2019ZD0402+7 种基金the National Key Research and Development Program under contract No.2018YFD0901204the Major Agricultural Application Technology Innovation Project of Shandong Province under contract No.SD2019YY011the Natural Science Foundation of Shandong Province under contract No.ZR2018BC053the Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology(Qingdao)under contract No.2018SDKJ0502-2the Fund of China Agriculture Research System under contract No.CARS-47the Major Science and Technology for Scientific and Technological Innovation Projects(Shandong)under contract No.2019JZZY020710the Science and Technology Service Network Initiative Project under contract Nos KFZD-SW-106,ZSSD-019,2017T3017 and 2019T3022the Advanced Talents Foundation of Qingdao Agricultural University under contract No.6631119055。
文摘In most fish,reproduction is seasonal or periodic under the suitable conditions.In turbot(Scophthalmus maximus)farms,one of the most economically important marine flatfish species,changes in daylength could cause changes in the spawning time.In this study,to characterize the regulation of reproductive physiology following light signals,three melatonin receptors(Mtnr)investigated in turbot were named sm Mtnr1,sm Mtnr2,and sm Mtnr1 c.Distinct expression profiles demonstrated that Mtnr m RNAs were concentrated in the brain(as detected in the hypothalamus(Hy)and mesencephalon(Me)),gonad and eye.The most abundant Mtnr1 and Mtnr2 m RNA expression levels were detected in the central nervous system at the beginning of the breeding season,suggesting that Mtnr1 and Mtnr2 may play vital roles in the regulation of turbot gonadal development.In addition,the melatonin profiles gradually increased and reached to the highest level at the spawning stage,indicating that melatonin is a potent hormone in the regulation of fish oocyte growth and maturation.The results of this study suggested that melatonin is the primary factor that transduces the light signal and regulates the physiological functions of turbot seasonal reproduction.Moreover,the results of this study may establish a foundation for further research seeking to identify fish melatonin receptors involved in the gonadal development and gamete maturation.
基金support of National Natural Science Foundation of China(Grant No.32070608).
文摘Dynamic DNA methylation represses transposable elements(TEs)and regulates gene activity,playing a pivotal role in plant development.Although substantial progress has been made in understanding DNA methylation reprogramming during germline development in Arabidopsis thaliana,whether similar mechanisms exist in other dicot plants remains unclear.Here,we analyzed DNA methylation levels in meiocytes,microspores,and pollens of Brassica Rapa using whole-genome bisulfite sequencing(WGBS).Global DNA methylation analysis revealed similar CHH methylation reprogramming compared to Arabidopsis,while distinct patterns were observed in the dynamics of global CG and CHG methylation in B.rapa.Differentially methylated region(DMR)analysis identified specifically methylated loci in the male sex cells of B.Rapa with a stronger tendency to target genes,similar to observations in Arabidopsis.Additionally,we found that the activity and genomic targeting preference of the small RNA-directed DNA methylation(RdDM)were altered during B.Rapa male germline development.A subset of long terminal repeat(LTR)TEs were activated,possibly due to the dynamic regulation of DNA methylation during male sexual development in B.Rapa.These findings provided new insights into the evolution of epigenetic reprogramming mechanisms in plants.
文摘Organic contaminants can be detected at low concentrations in drinking water, raising concerns for human health, particularly in reproduction. In this respect, we attempted to use the zebrafish as a bioindicator to detect the possible presence of these substances in drinking water, aiming to define the most relevant parameters to detect these substances, which particularly affect the development and reproduction of zebrafish. To this end, batches of30 embryos with the chorion intact were cultured in drinking waters from different sources,throughout their full life-cycle up to 5 months, in 20 L tanks. Six replicates were performed in all water groups, with a total of 24 aquariums. Two generations（F0 and F1） were studied and the following parameters were tested： in the F0 generation, survival and abnormality rates evaluated at 5 dpf（days post-fertilization） and at 5 mpf（months post-fertilization）,the onset of spawning and the fertility rate from 3 mpf to 5 mpf, and the sex ratio and underdeveloped specimens at 5 mpf. Furthermore, in the F0 offspring（F1）, survival and abnormality rates were evaluated at 5 dpf and the hatching rate at 72 hpf. These results revealed that the hatching rate is the most sensitive parameter to distinguish different levels of effects between waters during the early life stages, whereas the rate of underdeveloped specimens is more suitable at later life stages. Regarding adult reproduction, fertility rate was the most sensitive parameter. The possible reversibility or accumulative nature of such effects will be studied in future work.
基金supported by the Research Initiative of Development of Transgenic Cotton Plants funded by Ministry of Agriculture, China (2008ZX08005-004)
文摘A novel gene, GhSERK1, was identified in cotton. It encoded a protein belonging to the somatic embryogenesis receptor- like kinase （SERK） family. The genomic sequence of GhSERK1 was 6 920 bp in length, containing a predicted transcriptional start site （TSS）. Its full-length cDNA was 2 502 bp, encoding a protein of 627 amino acids. Sequence analysis of GhSERK1 revealed high levels of similarity to other reported SERKs, as well as a conserved intron/exon structure that was unique to members of the SERK family. Expression analysis showed that GhSERK1 mRNA was present in all organs of cotton plants and at different developmental stages, but its transcripts were most abundant in reproductive organs. Compared with that of the male-fertile line, the level of GhSERK1 mRNA was lower in the anther of the male-sterile cotton line, in which the pollen development was defected. Taken together, these findings illustrated that the GhSERK1 play a critical role during the anther formation, and may also have a broad role in other aspects of plant development.
文摘Female reproductive endocrinology plays an important role in obstetrics and gynecology, and is a component part of perinatology, family planning and geriatrics. It is closely linked with the physiological changes, disease conditions and health care in different reproductive stages of a woman’s life. Ovarian function consists of ovulation and hormonal secretion, which acts upon the uterine endometrium and other target organs and feeds back to the hypothalamus-pituitary unit. Thus, it is connected with other endocrine systems, cerebral cortex and the external environment. Whether the ovrian function is normal or not reflects itself clinically in the changes of menstrual cycle and reproductive status and may be evaluated by laboratory assays.
文摘Invertase （INV） hydrolyzes sucrose into glucose and fructose, thereby playing key roles in primary metabolism and plant development. Based on their pH optima and sub-cellular locations, INVs are categorized into cell wall, cytoplasmic, and vacuolar subgroups, abbreviated as CWlN, CIN, and VlN, respectively. The broad importance and implications of INVs in plant development and crop productivity have attracted enormous interest to examine INV function and regulation from multiple perspectives. Here, we review some exciting advances in this area over the last two decades, focusing on （1） new or emerging roles of INV in plant development and regulation at the post-translational level through interaction with inhibitors, （2） cross-talk between INV-mediated sugar signaling and hormonal control of development, and （3） sugar- and INV-mediated responses to drought and heat stresses and their impact on seed and fruit set. Finally, we discuss major questions arising from this new progress and outline future directions for unraveling mechanisms underlying INV-mediated plant development and their potential applications in plant biotechnology and agriculture.
基金supported by Conselho Nacional de Desenvolvimento Científico e Tecnológico(CNPq:406455/2022-8,305157/2023-0)Fundacao de AmparoàPesquisa do Estado de Minas Gerais(FAPEMIG:APQ-01942-22,BPD00823-22,RED-00060-23)。
文摘Extreme temperatures have become an unavoidable reality across vast regions of the world.Heat waves can cause severe crop damage,intensify drought conditions,and severely impact agriculture,disrupting food systems and exacerbating food insecurity(Zsögön et al.,2022).Heat stress adversely affects reproductive development in plants,leading to grain or ovary abortion and causing significant yield losses(Li et al.,2012).
文摘MY ancestors have lived in Lhasa, Tibet, for generations. I have personally witnessed the great changes in modern Tibetan society. The total population of the region has doubled since 1951. The Fourth National Census in 1990 revealed that the human fertility rate in the Tibet Autonomous Region was 27.60 per thousand, the natural population growth rate 18.40 per thousand, and the total fertility rate of women at childbearing age 4.22.
