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Research and development of the pulse acquisition system and the pulse biomimetic reproduction system
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作者 YI Kai MA Yunjing +2 位作者 GUO Shen LIU Heng XU Bin 《Journal of Traditional Chinese Medicine》 2025年第1期213-220,共8页
The paper is an introduction to the front-end pulse acquisition and the back-end pulse biomimetic reproduction system.This system is capable of faithfully replicating the complete pulse waveform collected at the front... The paper is an introduction to the front-end pulse acquisition and the back-end pulse biomimetic reproduction system.This system is capable of faithfully replicating the complete pulse waveform collected at the front end.Traditional Chinese Medicine(TCM)practitioners analyze and diagnose the pulse patterns at the replication end.Meanwhile,the obtained pulse waveforms are analyzed and learnt by a neural network based on key diagnostic points in TCM pulse taking,which enables the determination of the corresponding relationships between different pulse waveforms and various pulse patterns in TCM pulse taking.With the support of clinical samples,an auxiliary diagnostic system for TCM pulse patterns ensures the accuracy of pulse pattern replication. 展开更多
关键词 pulse taking medicine Chinese traditional pulse acquisition system pulse biomimetic reproduction system
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Insulin-like Growth Factor-I (IGF-I) in Reproduction System of Female Bovine
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作者 QI Meiyu ZviRoth LIU Di 《Journal of Northeast Agricultural University(English Edition)》 CAS 2011年第4期84-87,共4页
Insulin-like growth factor-I (IGF-I) plays a key role in female reproduction, because it has the effect of anti-apoptosis improving cell proliferation, transformation and differentiation. This paper reviewed the eff... Insulin-like growth factor-I (IGF-I) plays a key role in female reproduction, because it has the effect of anti-apoptosis improving cell proliferation, transformation and differentiation. This paper reviewed the effects of IGF-I on ovary, follicle growth, acquisition of oocyte competence and preimplantation embryo viability, and then summarized different points about IGF-1 for reproduction system 展开更多
关键词 IGF-1 BOVINE reproduction system OVARY follicle OOCYTE preimplantation embryo
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Unidirectional Sound Signage for Speech Frequency Range Using Multiple-Loudspeaker Reproduction System
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作者 Vecky Canisius Poekoel Koshiro Hira +1 位作者 Yoshifumi Chisaki Tsuyoshi Usagawa 《Open Journal of Acoustics》 2013年第4期120-126,共7页
Human safety is the most important issue in disaster management. Speech is a sound signal containing information that is easily and quickly understood by humans. Using speech as sound signage in emergency systems can ... Human safety is the most important issue in disaster management. Speech is a sound signal containing information that is easily and quickly understood by humans. Using speech as sound signage in emergency systems can effectively increase human safety in low or poor visibility conditions such as in smoke-filled situations. However, reflections of sound through walls, floor surfaces, and ceilings will affect clarity of speech. Unfortunately, because of the characteristics of sound reproduction systems, a single loudspeaker propagates sound waves omni-directionally at low frequencies. This paper proposes a simple multiple-loudspeaker system for reproducing sound with uni-directional characteristics. The proposed system consists mainly of a primary loudspeaker for introducing sound in the desired beam, a secondary loudspeaker for reducing gain in the undesired direction, and digital filters. An adaptive finite-impulse-response (FIR) filter is used to produce the controlling sound by implementing a filtered-x least-mean-square algorithm, and a delay filter for adjusting the time alignment of sound propagation between primary and secondary sources at the control point. Several operational conditions for illustrating real situations and reflections were considered in an anechoic chamber. Experimental results show the directivity patterns of the proposed multiple-loudspeaker system for the required conditions. In a low frequency range, the system is able to control unidirectional propagation;there is a sound beam in the desired direction and, conversely, reduction of gain in the undesired direction around the control point. 展开更多
关键词 Uni-Directional reproduction system Multiple-Loudspeaker system Adaptive Filter Propagation Low Frequency
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Mobile applications for patients undergoing assisted reproduction:A systematic review of the gap between clinical research and practice
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作者 Dang-Quang Tran Minh-Triet Ho +6 位作者 Thuy Thi-Thanh Tran Tuyet Thi-Diem Hoang Hang Thi Phan Nam Nhat Nguyen Van-Toi Vo Huy Phuong Tran Le-Giang Tran 《Asian pacific Journal of Reproduction》 2025年第6期244-257,共14页
Objective:To evaluate mobile applications available for patients undergoing assisted reproduction and assess the extent of their clinical validation.Methods:A systematic search was conducted in the Apple App Store and... Objective:To evaluate mobile applications available for patients undergoing assisted reproduction and assess the extent of their clinical validation.Methods:A systematic search was conducted in the Apple App Store and Google Play between September 1,2023 and September 30,2023 to identify mobile applications related to assisted reproduction.Apps were evaluated using the mobile app rating scale(MARS).In parallel,a literature search of PubMed,Scopus,Embase,and Web of Science was performed to identify clinical studies related to mobile applications in assisted reproduction.Clinical validation status and MARS scores were recorded,and findings were synthesized to highlight the gap between commercially available apps and research-based evidence.Results:From 1143 apps screened,11 met the inclusion criteria.Mean MARS score across apps was 3.63,with Leeaf scoring the highest(4.60).However,only one application(Embie)was supported by published research.The literature research identified 13 relevant studies,mostly randomized controlled trials,cohort studies,or usability studies.While research-based apps demonstrated clinical utility(e.g.,MediEmo,PreLiFe,Patient Journey App),most were unavailable on app stores.This revealed a disconnect between research-backed applications and those accessible to patients.Conclusions:Although several mobile apps for assisted reproduction demonstrate high usability and quality,few are clinically validated.The lack of integration between research and practice highlights the need for stronger collaboration between researchers,developers,and policymakers to ensure that patients access safe and effective tools. 展开更多
关键词 Mobile application mHealth apps Digital health tools In vitro fertilization Assisted reproduction INFERTILITY User experience Usability assessment
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How cushion communities are maintained in alpine ecosystems: A review and case study on alpine cushion plant reproduction 被引量:5
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作者 Jianguo Chen Yanbo Li +1 位作者 Yang Yang Hang Sun 《Plant Diversity》 SCIE CAS CSCD 北大核心 2017年第4期221-228,共8页
Cushion species occur in nearly all alpine environments worldwide. In past decades, the adaptive and ecosystem-engineering roles of such highly specialized life forms have been well studied. However, the adaptive stra... Cushion species occur in nearly all alpine environments worldwide. In past decades, the adaptive and ecosystem-engineering roles of such highly specialized life forms have been well studied. However, the adaptive strategies responsible for cushion species reproductive success and maintenance in severe alpine habitats remain largely unclear. In this study, we reviewed the current understanding of reproductive strategies and population persistence in alpine cushion species. We then present a preliminary case study on the sexual reproduction of Arenaria polytrichoides(Caryophyllaceae), a typical cushion species inhabiting high elevations of the Himalaya Hengduan Mountains, which is a hotspot for diversification of cushion species. Finally, we highlight the limitations of our current understanding of alpine cushion species reproduction and propose future directions for study. 展开更多
关键词 Alpine ecosystem Cushion plant Community recruitment Growth rate Life-span reproduction
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EXPERIMENT WITH ONE DIMENSIONAL DATA FOR REPRODUCTION OF ATTRACTOR STRUCTURE IN N-DIMENSIONAL SYSTEM
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作者 林振山 朱焰宇 《Journal of Tropical Meteorology》 SCIE 1997年第1期105-112,共8页
Illustrated with the Rossler and Lorenz systems, the topological structure is reproduced using univariable temporal series. The study shows that there are a number of principal vectors reproduced withone-dimensional t... Illustrated with the Rossler and Lorenz systems, the topological structure is reproduced using univariable temporal series. The study shows that there are a number of principal vectors reproduced withone-dimensional temporal series,which reflect from a certain aspect the topological structure for the system attractors. 展开更多
关键词 phase space principal VECTORS reproduction of TOPOLOGICAL STRUCTURE
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Non-Central Zone 3D Sound Field Reproduction for Multichannel System
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作者 WANG Song ZHANG Cong +2 位作者 PENG Bo WANG Heng HU Ruimin 《Wuhan University Journal of Natural Sciences》 CAS CSCD 2017年第6期498-502,共5页
The 22.2 multichannel system and its simplified system with 10-channel and 8-channel have been proposed, which brings people 3 D listening experience. But these systems could only accurately reproduce sound field at a... The 22.2 multichannel system and its simplified system with 10-channel and 8-channel have been proposed, which brings people 3 D listening experience. But these systems could only accurately reproduce sound field at a central listening point which is called sweetspot. In order to solve this problem, this paper proposes a non-central zone sound field reproduction method PVMDZ(particle velocity matching between different zones) based on the physical property of sound. The proposed method matches the physical property of sound of non-central zone in reconstructed sound field with that of central zone in original sound field, so the reproduced non-central zone would produce the same listening experience as the central zone of the original system does. By experiments, we compare the performances of the proposed method with the traditional one, and the result proves that the sound field error of proposed method is reduced. 展开更多
关键词 non-central zone sound field reproduction multichannel system
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Pharmacological actions of the bioactive compounds of Epimedium on the male reproductive system:current status and future perspective 被引量:2
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作者 Song-Po Liu Yun-Fei Li +10 位作者 Dan Zhang Chun-Yang Li Xiao-Fang Dai Dong-Feng Lan Ji Cai He Zhou Tao Song Yan-Yu Zhao Zhi-Xu He Jun Tan Ji-Dong Zhang 《Asian Journal of Andrology》 2025年第1期20-29,共10页
Compounds isolated from Epimedium include the total flavonoids of Epimedium,icariin,and its metabolites(icaritin,icariside Ⅰ,and icariside Ⅱ),which have similar molecular structures.Modern pharmacological research a... Compounds isolated from Epimedium include the total flavonoids of Epimedium,icariin,and its metabolites(icaritin,icariside Ⅰ,and icariside Ⅱ),which have similar molecular structures.Modern pharmacological research and clinical practice have proved that Epimedium and its active components have a wide range of pharmacological effects,especially in improving sexual function,hormone regulation,anti-osteoporosis,immune function regulation,anti-oxidation,and anti-tumor activity.To date,we still need a comprehensive source of knowledge about the pharmacological effects of Epimedium and its bioactive compounds on the male reproductive system.However,their actions in other tissues have been reviewed in recent years.This review critically focuses on the Epimedium,its bioactive compounds,and the biochemical and molecular mechanisms that modulate vital pathways associated with the male reproductive system.Such intrinsic knowledge will significantly further studies on the Epimedium and its bioactive compounds that protect the male reproductive system and provide some guidances for clinical treatment of related male reproductive disorders. 展开更多
关键词 EPIMEDIUM ICARIIN INFERTILITY male reproductive system TUMOR
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Perspective for Vegetative Reproduction of Eleaagnus macrophylla Thunb Species and Shepherdia canadensis (L.) Nutt
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作者 Norkulova Lobar Uchkunovna Haydarov Khislat Kudratovich 《American Journal of Plant Sciences》 2025年第1期22-27,共6页
Elaeagnaceae Juss. belongs to the family Elaeagnus L. and Shepherdia Nutt. This study aims at the significance and vegetative reproduction of species of the family (Elaeagnus macrophylla Thunb. and Shepherdia canadens... Elaeagnaceae Juss. belongs to the family Elaeagnus L. and Shepherdia Nutt. This study aims at the significance and vegetative reproduction of species of the family (Elaeagnus macrophylla Thunb. and Shepherdia canadensis (L.) Nutt.) in the national economy. The trials were carried out in the experimental site of SamSU’s Botanic Garden, which is named after Sharof Rashidov. The study’s findings are based on the results obtained by planting cuttings of S. canadensis and E. macrophylla species of varying sizes and durations, as well as the cuttings’ roots and the impacts of growth chemicals. 展开更多
关键词 ELAEAGNACEAE ELAEAGNUS Shepherdia Vegetative reproduction ATTRACTIVENESS Bioactive Compound BUSH and Cuttings
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Supplementation of high levels of essential fatty acids using soybean oil in lactation diets benefits the subsequent reproduction of sows but can be detrimental to the performance of young sows if provided after weaning
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作者 Garrin Lee Shipman David Rosero Eric van Heugten 《Journal of Animal Science and Biotechnology》 2025年第4期1704-1717,共14页
Background This study investigated the potential impacts of increasing linoleic andα-linolenic acid intake during lactation and wean-to-breeding on subsequent reproduction of sows.A total of 309 sows(PIC Camborough L... Background This study investigated the potential impacts of increasing linoleic andα-linolenic acid intake during lactation and wean-to-breeding on subsequent reproduction of sows.A total of 309 sows(PIC Camborough L42)were balanced by parity(140 and 169 sows representing parity 1 to 2[P1-2]and 3 to 9[P3+],respectively)and assigned within parity to a 2×2 factorial arrangement.Factors included essential fatty acid(EFA)supplementation(control diets containing 1.2%linoleic and 0.15%α-linolenic acid or diets with 3.0%linoleic and 0.38%α-linolenic acid)and supplementation period(lactation or wean-to-breeding).Tallow(low EFA diets)or soybean oil(high EFA diets)were included at 4%in sorghum-soybean meal-wheat middlings-based diets to attain targeted EFA levels.Results High levels of EFA fed during lactation had no effect on feed intake or litter performance,but increased subsequent farrowing rate(P=0.027;82.1%vs.70.4%),tended to reduce the proportion of sows removed(P=0.070;12.4%vs.20.8%),decreased the number of total pigs born in the following litter(P=0.072;15.3 vs.16.2),and increased total pigs born alive per 100 sows weaned(P=0.062;1,122 vs.974),regardless of sow parity.Young sows(P1-2)consuming the high EFA diet during lactation displayed a shorter wean-to-estrus interval(P=0.035;4.2 vs.4.6),but P3+sows were unaffected.Increasing EFA intake for P3+sows,but not P1-2 sows,resulted in more sows bred by d 5(P=0.028;91.1%vs.81.7%)and more mummies in the subsequent litter(P=0.040;0.32 vs.0.16).Feeding increased EFA to P1-2 sows during the wean-to-breeding period decreased subsequent farrowing rate(P=0.042;72.0%vs.87.7%),and increased removal rate(P=0.003;28.8%vs.9.4%).Total pigs born alive per 100 sows weaned was reduced(P=0.007)in P1-2 sows when supplemented with EFA during wean-breeding(939 vs.1,149)but was not impacted in P3+sows(1,131 vs.982).Conclusions Supplemental EFA in lactation diets benefited subsequent reproduction of sows,regardless of parity.Increasing dietary levels of EFA during the wean-to-breeding period to younger sows negatively impacted subsequent reproduction. 展开更多
关键词 α-Linolenic acid LACTATION Linoleic acid SOWS Subsequent reproduction Wean-to-breeding
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Environmental stability via sexual reproduction of cyclical parthenogenetic rotifers affects offspring's population performances:maternal effects on population dynamics
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作者 Huanhuan XU Yingying GE +1 位作者 Kangning LIU Cuijuan NIU 《Journal of Oceanology and Limnology》 2025年第6期1892-1901,共10页
Environmental fluctuations are a pervasive natural phenomenon that typically favor diverse maternal investment strategies.Organisms across various evolutionary lineages have acquired the ability to enter the dormancy ... Environmental fluctuations are a pervasive natural phenomenon that typically favor diverse maternal investment strategies.Organisms across various evolutionary lineages have acquired the ability to enter the dormancy state to tolerate conditions that are unsuitable for normal life activities.In cyclical parthenogenesis,sexual reproduction is generally linked with dormancy,thereby enhancing the long-term fitness of populations through positive feedback mechanisms.This study evaluates the impact of environmental variability on offspring population dynamics through sexual reproduction in the rotifer Brachionus calyciflorus.We found that mictic females(sexual females)exposed to low-predictability environments produced resting eggs with a sporadic hatching pattern,reduced hatching rate,and smaller sizes of resting egg,embryo,and stem females,while increasing variations in fertilization among individuals to maximize population fitness.Variations in population growth could be manifested in enhanced reproductive capacity 2–3 d post-hatching,along with differences in fecundity among individuals.This suggests that dormant eggs from unstable environments may prioritize offspring survival and adjust to increased fertilization among individuals.This adaptive strategy enables rotifer populations to rapidly adjust to environmental changes,thus avoiding extinction and thriving in diverse habitats.Our systematic study enhances the understanding of how environmental stability affects long-term population fitness in cyclical parthenogens. 展开更多
关键词 environmental stability maternal effect sexual reproduction population regulation Brachionus calyciflorus ZOOPLANKTON
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Plasticity,Contingency,and Virtuality in the Age of Automatic Reproduction
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作者 Szymon Wróbel 《Philosophy Study》 2025年第4期135-144,共10页
In this paper,the author follows the trail of C.Malabou,Q.Meillassoux,and G.Deleuze and tries to test three philosophical concepts that seem to be particularly threatened in the era of automatic digital reproduction.T... In this paper,the author follows the trail of C.Malabou,Q.Meillassoux,and G.Deleuze and tries to test three philosophical concepts that seem to be particularly threatened in the era of automatic digital reproduction.These three concepts are plasticity(defended for many years by C.Malabou),contingency(reconstructed by Q.Meillassoux),and virtuality(developed by G.Deleuze).The main task of the text will be to reflect on which of these three concepts better protects our thinking against automation and stays faithful to the ideal of creativity.In what sense are plasticity,contingency,and the possibility of virtualization the a priori condition of any transformation,physical or intellectual,affective or conceptual metamorphosis?In what sense are these three concepts the only conditions for the survival of every living being?Would a being without contingency,plasticity,and disposition to virtualization simply be a dead being? 展开更多
关键词 automatic society CONTINGENCY PLASTICITY technical reproduction VIRTUALIZATION
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The Limitations of Social Reproduction Theory in Explaining Social Unpaid Work in the Global South
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作者 Yuqian Liu 《Journal of Contemporary Educational Research》 2025年第1期176-179,共4页
This paper defines and explores the concept of social reproduction work,highlighting its significance and the fact that women are its primary contributors.It identifies key social issues faced by social reproduction w... This paper defines and explores the concept of social reproduction work,highlighting its significance and the fact that women are its primary contributors.It identifies key social issues faced by social reproduction workers,including marginalization,neglect,and unequal access to resources.Additionally,the study examines the primary challenges encountered by these workers:the lack of direct economic benefits,difficulty in balancing work and family responsibilities,and the significant physical and mental strain associated with unpaid labor.Using the theoretical framework of the capitalist economic system,the paper delves into the root causes of the caregiving crisis.Furthermore,it critiques the applicability of reproduction work theories in explaining unpaid care work in the Global South.Three key limitations are outlined:the neglect of informal economic systems in the Global South,persistent gender inequality,and the dynamic nature of global economic migration. 展开更多
关键词 SOCIOLOGY Social reproduction Care work
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Practice and hygiene of adolescent reproduction health in agricultural area:a qualitative study
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作者 Tantut Susanto Ira Rahmawati +4 位作者 Raudlatul Jannah Pratiwi Puspitho Andini Nuning Dwi Merina Wahyuni Fauziah Alice Yuen Loke 《Frontiers of Nursing》 2025年第1期27-36,共10页
Objective: Adolescent reproduction health (ARH) differs greatly based on religions, religious norms, culture, and ethnicities in the Indonesian context. The value of local wisdom was considered too binding, traditiona... Objective: Adolescent reproduction health (ARH) differs greatly based on religions, religious norms, culture, and ethnicities in the Indonesian context. The value of local wisdom was considered too binding, traditional, and outdated, particularly on ARH in agricultural areas. The purpose of the study was to identify the problems, challenges, and needs, specifically for ARH, in the agricultural area of Indonesia. Methods: A qualitative study was performed among 41 communities, including 28 village officials (VOs), 9 community health volunteers (CHVs), and 9 adolescents. The data were collected through in-depth interviews and FGD. An thematic analysis was used to describe the phenomena. Results: This research obtained results regarding ARH in terms of communication patterns, daily behavior, reproductive practices, and hygiene of adolescent reproductive organs, especially in agricultural areas. Conclusions: The differences in the perspective of ARH are influenced by environment, parental roles, religion, ethnicity, and knowledge. 展开更多
关键词 ADOLESCENT local wisdom reproductive health practice hygiene AGRICULTURAL
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A narrative review of spermbots in assisted reproduction:Integrating AI for enhanced fertility outcomes and future innovations
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作者 Neeraj Vishwakarma Nancy Nair +1 位作者 Charu Pareek Namrata Choudhary 《Asian pacific Journal of Reproduction》 2025年第3期99-104,共6页
Sperm-structure-integrating nanodecorated microrobots have shown promise in medicine delivery and infertility treatment.A variety of spermbots use cutting-edge nanomaterials and 3D printing technology to enhance their... Sperm-structure-integrating nanodecorated microrobots have shown promise in medicine delivery and infertility treatment.A variety of spermbots use cutting-edge nanomaterials and 3D printing technology to enhance their functioning,such as biomimetic sperms and flagellate microorganisms.The success rates of assisted reproductive technology techniques like in vitro fertilisation(IVF)and intracytoplasmic sperm injection(ICSI)may increase as a result of these developments.Furthermore,the incorporation of artificial intelligence(AI)into spermbots has the potential to optimize reproductive therapies by reducing inherited illnesses through genetic screening and editing.However,before the widespread implementation of spermbots in clinical practice,several critical aspects must be addressed.Thorough investigations into biocompatibility,ethical considerations,and long-term safety are necessary to ensure that these technologies are safe and effective for in vivo applications. 展开更多
关键词 Spermbots Micro robotics Artificial intelligence(AI) Assisted reproductive technology(ART) Intracytoplasmic sperm injection(ICSI) Male infertility
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Male Reproductive System and Spermatogenesis in Homoptera(Insecta:Hemiptera) 被引量:9
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作者 田润刚 袁锋 张雅林 《Entomotaxonomia》 CSCD 北大核心 2006年第4期241-253,共13页
Morphology of the male reproductive system, chromosome behaviors during meiosis and spem tail structures in Homoptera and Heteroptera are compared in this paper. The sheathed testis is found in Fulgoroidea and Heterop... Morphology of the male reproductive system, chromosome behaviors during meiosis and spem tail structures in Homoptera and Heteroptera are compared in this paper. The sheathed testis is found in Fulgoroidea and Heteroptera, and unsheathed testis occurs in Cicadoidea, Cicadelloidea, Cercopoidea, Membracoidea, Psyloidea, Aphidoidea, Aleyrodoidea and Coccoidea. The testis also can be divide into three types by the shape of testicular follicles. The sphere-shaped type is found in Cicadoidea, Cicadelloidea, Cercopoidea, Membracoidea, Aphidoidea and Aleyrodoidea, the tube-shaped type observed in Fulgoroidea, Psyloidea and Coccoidea, and the lamella-shaped type represented by Heteroptera. It is suggested the unsheathed testis may be the primitive type in Homoptera. Meiosis can be divided into 6 type at least, i.e. 1) Cicadoid type; 2) Fulgoroid type; 3) Psyloid type; 4) Aphidoid type; 5) Aleyrodoid type; and 6) Coccoid type. At least four groups exhibit a diffuse stage during meiosis prophase l, they are Psyloidea, Fulgoroidea, Coccoidea and Heteroptera. Sperm tail structures are similar to those reported from other insects with a typical 9+9+2 axoneme except that in Aleyrodoidea and Coccoidea whose sperm tail is degenerated. 展开更多
关键词 HEMIPTERA HOMOPTERA reproductive system SPERMATOGENESIS MEIOSIS sperm structure
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ISSUES AND MEASURES ON ASSISTED REPRODUCTION BY ACUPUNCTURE ABROAD 被引量:2
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作者 彭艳 丛晶 +1 位作者 侯丽辉 吴效科 《World Journal of Acupuncture-Moxibustion》 2007年第2期31-34,共4页
As the application of acupuncture has been increased year by year in the field of overseas reproduction, it has become crucial to give a scientific and objective evaluation of the safety and efficacy of acupuncture in... As the application of acupuncture has been increased year by year in the field of overseas reproduction, it has become crucial to give a scientific and objective evaluation of the safety and efficacy of acupuncture in the field of assisted reproduction, which attaches great importance to the sustainable development of acupuncture in the field of reproduction. This article aims to introduce the current situation of overseas clinical study in the field of assisted reproduction, analyze the existing issues profoundly and put forward measures to solve them, in order to enhance the level of international clinical study of acupuncture. 展开更多
关键词 Acupuncture Assisted reproduction Clinical study
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Research on Development Mode of Solid Ancient Towns Based on Cultural Reproduction——A Case Study of Zouma Ancient Town in China 被引量:1
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作者 陈琴 李俊 张述林 《Journal of Landscape Research》 2011年第8期28-31,共4页
The paper had pointed out hollow ancient town was the result of urbanization and desire; and analyzed that hollowness made ancient towns degenerate in nature, economy and social function, seriously blocking the sustai... The paper had pointed out hollow ancient town was the result of urbanization and desire; and analyzed that hollowness made ancient towns degenerate in nature, economy and social function, seriously blocking the sustainable development of economic society of ancient towns. Based on the connotation and idea of cultural reproduction, in terms of application of basic theories of cultural ecology and restoration ecology, it had established tourism development of solid ancient towns, which had been analyzed specifically from the perspectives of representation of ancient towns'scene, cultural realization and cultural promotion. By taking Zouwa Ancient Town in the west of the main urban area of Chongqing City for example, it had discussed tourism development practice of ancient towns, emphasized making Zouma scene reappear from the aspects of pattern protection, restoration and treatment of ecological environment, ancient towns' protection and cultural facilities' construction, experiencing and publicizing Zouma culture. It also highlighted taking historical and cultural heritages as the theme of tourist products, and proposed development mode of solid ancient towns, realizing reproduction of cultural heritage. 展开更多
关键词 HOLLOW ANCIENT TOWNS CULTURAL reproduction Development mode Solidness
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Advances in Small RNAs and Sexual Reproduction in Plants
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作者 王俊 何桥 +5 位作者 汪卫星 向素琼 孙海艳 李晓林 梁国鲁 郭启高 《Agricultural Science & Technology》 CAS 2013年第2期211-214,229,共5页
Small RNAs are non-coding RNA molecules with 20-30 nucleotides (nt) in length that mainly play regulatory roles in gene expression at the post-transcription level by directly cutting target mRNA or inhibiting its tr... Small RNAs are non-coding RNA molecules with 20-30 nucleotides (nt) in length that mainly play regulatory roles in gene expression at the post-transcription level by directly cutting target mRNA or inhibiting its translation. Small RNAs play regulatory roles in the growth and development process of plants at the core of gene regulatory networks, which has been widely studied and confirmed in sporophyte generation of plants. However, few researches have been conducted on small RNAs and gametophyte generation. It is reported that small RNAs play important roles in floral organ development, gametogenesis, fertilization, and early zygotic development of plants. In addition, various small RNAs also play roles in controlling genetic integrity, cell differentiation and functions during the sexual reproduction process of plants. However, most of the specific functions of small RNAs in the sexual reproduction process are unknown yet. This study mainly aimed to introduce small RNAs in plants, summarize the latest advances in researches of small RNAs and plant sexual reproduction, and make prospect on its future. 展开更多
关键词 Small RNA Sexual reproduction Floral organ development GAMETOGENESIS FERTILIZATION Early zygotic development
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Development of an Improved DNA-launched Porcine Reproductive and Respiratory Syndrome Virus Reverse Genetics System
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作者 刘长龙 李燕华 袁世山 《Agricultural Science & Technology》 CAS 2010年第6期32-36,共5页
[Objective] This study was to improve the virus replication efficiency of full length infectious cDNA clones by making use of the ribozyme's self incision property.[Method] By employing three-step PCR,HDV ribozyme(H... [Objective] This study was to improve the virus replication efficiency of full length infectious cDNA clones by making use of the ribozyme's self incision property.[Method] By employing three-step PCR,HDV ribozyme(HdvRz)cDNA was isolated,and cloned into the downstream flanking the genome of the porcine reproductive and respiratory syndrome virus,and into which the bovine growth hormone polyadenylation sequence(BGH)was inserted via enzyme digestion and ligation,yielding pAPRRS-HB.The newly constructed pAPRRS-HB was used to transfect MARC-145 cells,in which the N protein and non-structural protein(nsp2)were determined by indirect immunofluorescence assay after 72 h of expression;meanwhile the virus titer of cell supernatant was tested using TCID50 assay.[Result] pAPRRS-HB containing complete infectious PRRSV cDNA has been successfully developed,and it performed about 10-fold higher virus rescue rate than pAPRRS without the engineered ribozyme element.[Conclusion] The results laid a foundation for revealing the structure and function of PRRSV gene. 展开更多
关键词 Porcine reproductive and respiratory syndrome virus Full-length cDNA clone HDV ribozyme DNA-launched transfection
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