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m^(5)C RNA甲基化修饰与前庭功能异常和老年性耳聋的相关性研究
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作者 周旭 司峰志 《河北医学》 2026年第1期117-122,共6页
目的:探讨m^(5)C RNA甲基化修饰在前庭功能和老年性耳聋中的相关性。方法:总计24只近交系C57BL/6J小鼠,5月龄(成年小鼠组)、12月龄(中年小鼠组)、20月龄(老年小鼠组)各8只。听觉脑干反应阈值分析评价小鼠听力。DCFH-DA(二氯二氢荧光素-... 目的:探讨m^(5)C RNA甲基化修饰在前庭功能和老年性耳聋中的相关性。方法:总计24只近交系C57BL/6J小鼠,5月龄(成年小鼠组)、12月龄(中年小鼠组)、20月龄(老年小鼠组)各8只。听觉脑干反应阈值分析评价小鼠听力。DCFH-DA(二氯二氢荧光素-乙酰乙酸酯)法测定耳蜗和前庭组织ROS水平。TAMRA探针法测定其中组织线粒体DNA的完整性。m^(5)C RNA甲基化修饰免疫共沉淀法分析小鼠耳蜗和前庭组织GRM7 mRNA m^(5)C水平。Western blot法测定其中NSUN2的水平。结果:与成年小鼠组和中年小鼠组相比,老年小鼠组在8、16和32 kHz时的平均ABR阈值升高(P<0.05);耳蜗组织和前庭组织ROS相对水平均升高(P<0.05);含CD mtDNA占比(%)均降低(P<0.05);NSUN2相对表达水平均降低(P<0.05);GRM7 Input mRNA相对表达水平均降低(P<0.05);GRM7 m^(5)C mRNA相对表达水平降低(P<0.05)。老年小鼠组,与耳蜗组织组相比,前庭组织组ROS相对水平较低(P<0.05),GRM7 Input mRNA和GRM7 m^(5)C mRNA相对表达水平较高(P<0.05)。结论:m^(5)C甲基化修饰RNA水平下调及NSUN2表达水平降低与前庭功能和老年性耳聋相关。 展开更多
关键词 老年性耳聋 前庭功能 c57BL/6J小鼠 m^(5)c RnA甲基化修饰 nOP2/Sun RnA甲基转移酶2
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C/N复合诱导对硫酸盐还原菌(SRB)EPS的显著影响:空间分布、化学组成、Cu(Ⅱ)吸附性能及其机制
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作者 郑广文 宋卫锋 +8 位作者 杨佐毅 涂传英 周克勤 边俊铭 彭景峰 袁槟 李圣乐 张祥丹 白晓燕 《环境科学学报》 北大核心 2026年第1期36-50,共15页
胞外聚合物(EPS)能够有效吸附重金属并降低其环境毒性.LB(Loosely-Bound)-EPS和TB(Tightly-Bound)-EPS在吸附过程中表现出不同的作用.本研究探讨了梯度C/N体系对Desulfovibrio desulfuricans subsp.desulfuricans的诱导效应,分析了LB-EP... 胞外聚合物(EPS)能够有效吸附重金属并降低其环境毒性.LB(Loosely-Bound)-EPS和TB(Tightly-Bound)-EPS在吸附过程中表现出不同的作用.本研究探讨了梯度C/N体系对Desulfovibrio desulfuricans subsp.desulfuricans的诱导效应,分析了LB-EPS和TB-EPS在不同C/N条件下的组成及其对Cu(Ⅱ)的吸附性能.在最佳条件诱导(C/N=5)下,TB-EPS和LB-EPS的产量相比对照组分别增加了151.08%和170.03%;其中,LB-EPS以多糖为主,而TB-EPS则以蛋白质为主,TB-EPS的Cu(Ⅱ)吸附能力优于LB-EPS.吸附过程符合Langmuir等温模型,理论最大吸附量分别为1447.51 mg·g^(-1)和2232.30 mg·g^(-1),并遵循准二级动力学模型.FTIR、XPS和3D-EEM分析表明,诱导处理增强了EPS中蛋白类物质及其官能团的含量.XPS分析显示EPS化学组成发生显著变化,LB-EPS中C=O和C-O/C-N基团分别增加了67.19%和27.23%,TB-EPS中分别增加了53.19%和37.50%.诱导后,两种EPS中的酸性氨基酸(Asp和Glu)显著增加,且TB-EPS含量更高、提升更显著,为Cu(Ⅱ)提供了丰富的结合位点.以上所有定量数据和表征结果均表明,TB-EPS相较于LB-EPS具有更优的吸附性能.本研究为重金属污染废水治理提供了理论基础,并阐明了SRB的EPS的空间结构及功能机制. 展开更多
关键词 c/n诱导 LB-EPS TB-EPS 生物吸附 SRB
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低C/N废水对人工湿地脱氮效果及温室气体排放的影响
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作者 刘桃涓 樊欣雨 +1 位作者 赵龙 刘奋武 《农业环境科学学报》 北大核心 2026年第2期468-475,共8页
为探究湿地植物在低C/N废水中对人工湿地脱氮效果的强化作用及对温室气体排放效果的调控,以优化人工湿地的脱氮效能与温室气体减排能力,本试验以人工湿地小试系统为研究对象,研究了低C/N进水条件下,种植湿地植物(风车草)处理组与未种植... 为探究湿地植物在低C/N废水中对人工湿地脱氮效果的强化作用及对温室气体排放效果的调控,以优化人工湿地的脱氮效能与温室气体减排能力,本试验以人工湿地小试系统为研究对象,研究了低C/N进水条件下,种植湿地植物(风车草)处理组与未种植物的对照组对人工湿地的脱氮效率及体系的温室气体(Greenhouse gases,GHGs)排放规律。结果表明:湿地植物的种植有助于提高人工湿地脱氮效率,随着进水C/N的降低,人工湿地对总氮(TN)、NO_(3)^(-)-N的去除率下降。人工湿地系统对NH_(4)^(+)-N的去除效果在不同C/N进水条件下无明显差异。当进水C/N为6时,人工湿地的脱氮效果最佳,对TN、NO_(3)^(-)-N、NH_(4)^(+)-N的去除率分别为92.82%、66.24%、95.00%,对化学需氧量的去除率达73.61%。此外,人工湿地中N_(2)O的排放通量随进水C/N的提高而增加,而CH_(4)的排放通量则呈现下降的趋势,且湿地植物的种植降低了N_(2)O和CH_(4)的排放通量。研究表明,湿地植物的种植提高了人工湿地的脱氮效率,同时降低了人工湿地的温室气体排放量,表明湿地植物可优化脱氮效能与调控温室气体排放,实现低C/N污水的高效处理。 展开更多
关键词 人工湿地 c/n 湿地植物 温室气体排放 脱氮效率
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激光熔覆原位生成Ti(C,N)增强梯度涂层的组织与性能
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作者 齐勇田 廖宝毅 +1 位作者 陈瀚 邹增大 《激光技术》 北大核心 2026年第1期57-63,共7页
为了探究梯度涂层其品质与结构间的关联性,利用激光技术在金属基体表面制备出原位自生Ti(C, N)陶瓷相强化的梯度复合涂层,借助显微结构表征手段和显微硬度计,对梯度涂层的组织成分和性能进行了分析与研究。结果表明,涂层各层界面间均呈... 为了探究梯度涂层其品质与结构间的关联性,利用激光技术在金属基体表面制备出原位自生Ti(C, N)陶瓷相强化的梯度复合涂层,借助显微结构表征手段和显微硬度计,对梯度涂层的组织成分和性能进行了分析与研究。结果表明,涂层各层界面间均呈现出良好的冶金结合状态;铁基复合熔覆层的显微组织呈现α-Fe基体中弥散分布着原位合成的Ti(C, N)强化相,其基体结构以胞状树枝晶为主;Ti(C, N)粒子形态多呈尺寸不等的菱形、圆形或其它不规则形状,较均匀弥散分布溶入到熔覆层的基体中;原位新生成的Ti(C, N)陶瓷颗粒使熔覆层的显微硬度得到显著提升;硬度测试显示涂层呈梯度分布,铁基工作层平均硬度达880 HV_(0.2),过渡层约635 HV_(0.2),基体热影响区约550 HV_(0.2)。本研究对拓展激光熔覆技术在表面改性领域的工业应用有一定指导作用。 展开更多
关键词 激光技术 材料 梯度涂层 Ti(c n)颗粒
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Nb、Al复合微合金化对Mn-Cr齿轮钢高温渗碳晶粒度的影响
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作者 李海洋 郭子强 +3 位作者 秦萱 张东 周蕾 邓伟 《金属热处理》 北大核心 2026年第2期145-152,共8页
设计了Mn-Cr系Nb和Nb+Al微合金化齿轮钢,通过模拟热锻-正火-伪渗碳工艺,分析了试验钢热锻变形后冷却过程中的相变行为、正火工艺对组织的影响以及高温渗碳时的抗晶粒粗化能力,并基于ThermoCalc软件计算了Nb(C,N)和AlN的析出动力学。结... 设计了Mn-Cr系Nb和Nb+Al微合金化齿轮钢,通过模拟热锻-正火-伪渗碳工艺,分析了试验钢热锻变形后冷却过程中的相变行为、正火工艺对组织的影响以及高温渗碳时的抗晶粒粗化能力,并基于ThermoCalc软件计算了Nb(C,N)和AlN的析出动力学。结果表明,Nb钢在余热正火条件下可满足960℃×4 h渗碳的晶粒度要求,但等温正火时铁素体+珠光体组织细化,导致在960℃×4 h伪渗碳时发生了奥氏体异常晶粒长大。Nb+Al钢可同时满足960℃×4 h、980℃×4 h渗碳的晶粒度要求,未产生混晶缺陷。相对于Nb钢,Nb+Al钢在伪渗碳时表现出更佳的抗晶粒粗化或混晶能力,且与模拟锻造后的冷却速率关联性不强,这主要来源于其碳氮化物的体积分数更高以及碳氮化物析出行为对锻后冷却的适应性。 展开更多
关键词 齿轮钢 伪渗碳 异常晶粒长大 微合金化 nb(c n) ALn
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血清NT-proBNP、RDW-CV、hsCRP及HRV指标对HFrEF患者MACE发生的预测价值
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作者 陈统 崔建蕾 《江苏医药》 2026年第1期19-23,共5页
目的探讨血清N末端脑钠肽前体(NT-proBNP)、红细胞分布宽度变异系数(RDW-CV)、超敏CRP(hsCRP)及心率变异性(HRV)指标对射血分数降低的心力衰竭(HFrEF)患者主要不良心血管事件(MACE)发生的预测价值。方法根据治疗后随访3个月是否发生MACE... 目的探讨血清N末端脑钠肽前体(NT-proBNP)、红细胞分布宽度变异系数(RDW-CV)、超敏CRP(hsCRP)及心率变异性(HRV)指标对射血分数降低的心力衰竭(HFrEF)患者主要不良心血管事件(MACE)发生的预测价值。方法根据治疗后随访3个月是否发生MACE,106例HFrEF患者分为发生MACE组(33例)和未发生MACE组(73例)。比较两组入院时血清NT-proBNP、RDW-CV、hsCRP水平及HRV指标[连续24 h内正常RR间期的标准差(SDNN)、RR间期均值的标准差(SDANN)、相邻RR间期差值的均方根(RMSSD)和相邻RR间期差值>50 ms的百分比(PNN50%)]。ROC曲线分析血清NT-proBNP、RDW-CV、hsCRP及HRV指标SDNN、SDANN、RMSSD、PNN50%对HFrEF患者MACE发生的预测价值。结果发生MACE组NT-proBNP、RDW-CV及hsCRP水平高于未发生MACE组(P<0.05)。发生MACE组HRV指标SDNN、SDANN、RMSSD、PNN50%均低于未发生MACE组(P<0.05)。ROC曲线分析结果显示,血清NT-proBNP、RDW-CV、hsCRP及HRV指标SDNN、SDANN、RMSSD、PNN50%预测HFrEF患者MACE发生的AUC分别为0.721、0.801、0.737、0.929、0.932、0.729、0.605。结论血清NT-proBNP、RDW-CV、hsCRP及HRV指标SDNN、SDANN、RMSSD、PNN50%检测对HFrEF患者MACE发生有一定预测价值,值得临床关注并用于高风险人群的早期筛查与风险防控。 展开更多
关键词 射血分数降低的心力衰竭 主要不良心血管事件 n末端脑钠肽前体 红细胞分布宽度变异系数 超敏c反应蛋白 心率变异性
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The Dynamics of Relation Oat Panicle with Grain Yield by Nitrogen
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作者 Rubia Diana Mantai José Antonio Gonzalez da Silva +5 位作者 Emilio Ghisleni Arenhardt Airam Tereza Zago Romcy Sausen Manuel Osório Binello Vidica Bianchi Dionatas Rodrigues da Silva Luiz Michel Bandeira 《American Journal of Plant Sciences》 2016年第1期17-27,共11页
The oat panicle components were changed by the forms of nitrogen use influencing productivity. The aim of the study was to determine the direct and indirect effects of oat panicle components on grain yield by N-fertil... The oat panicle components were changed by the forms of nitrogen use influencing productivity. The aim of the study was to determine the direct and indirect effects of oat panicle components on grain yield by N-fertilizer rates in succession systems of high and reduced release of N-residual. The study was conducted in the years 2013 and 2014 in a randomized complete block with four replications in a factorial 3 × 2, for nitrogen rates (30, 60 and 120 kg·ha<sup>-1</sup>) and oat cultivars (Barbarasul and Brisasul) the soybean/oat and corn/oat systems. The panicle weight and grain per panicle show greater sensitivity to the change of nitrogen rates. Grain yield was favored by the direct effect of harvest index panicle and indirect by grain weight per panicle. In the system soybean/ oat, the grain weight of panicle shows greater efficiency in promoting positive direct effects on grain yield. 展开更多
关键词 Avena sativa L. Ureia relation c/n correlation and Path Analysis
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Stanford A型主动脉夹层动脉瘤患者手术前后血清CRP、SFRP5、Ficolin-3水平变化及对预后的影响
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作者 周壮 段俊成 +1 位作者 陈权 王浩 《检验医学与临床》 2026年第3期338-344,共7页
目的探讨Stanford A型主动脉夹层动脉瘤(ADA)患者手术前后血清C反应蛋白(CRP)、分泌型卷曲相关蛋白5(SFRP5)、纤维凝胶蛋白(Ficolin)-3水平变化及对预后的影响。方法选取2021年8月至2023年8月川北医学院附属医院收治的110例和四川省遂... 目的探讨Stanford A型主动脉夹层动脉瘤(ADA)患者手术前后血清C反应蛋白(CRP)、分泌型卷曲相关蛋白5(SFRP5)、纤维凝胶蛋白(Ficolin)-3水平变化及对预后的影响。方法选取2021年8月至2023年8月川北医学院附属医院收治的110例和四川省遂宁市中心医院收治的90例进行手术治疗的Stanford A型ADA患者作为研究组(共200例),另选取同期在川北医学院附属医院或四川省遂宁市中医院体检的健康体检者100例作为对照组,比较研究组术前和对照组血清CRP、SFRP5、Ficolin-3水平。术后随访1年,根据预后情况将研究组患者分为预后不良组和预后良好组,并比较预后良好组和预后不良组的临床资料。采用非条件二元Logistic回归分析Stanford A型ADA患者预后不良的影响因素。采用受试者工作特征(ROC)曲线分析ΔCRP、ΔSFRP5、ΔFicolin-3单独及联合检测对Stanford A型ADA患者预后不良的预测价值。结果研究组术前血清CRP水平高于对照组,血清SFRP5、Ficolin-3水平低于对照组,差异均有统计学意义(P<0.05)。研究组术后随访期间失访8例,最终有192例患者完成随访,其中41例患者预后不良,151例患者预后良好,预后不良率为21.35%(41/192)。预后不良组年龄、延迟关胸占比、术前及术后3 d血清CRP水平高于预后良好组,术前及术后3 d血清SFRP5、Ficolin-3水平,以及ΔCRP、ΔSFRP5、ΔFicolin-3低于预后良好组,差异均有统计学意义(P<0.05)。非条件二元Logistic回归分析结果显示,ΔCRP、ΔSFRP5、ΔFicolin-3降低是Stanford A型ADA患者预后不良的危险因素(P<0.05)。ROC曲线分析结果显示,ΔCRP、ΔSFRP5、ΔFicolin-3单独预测Stanford A型ADA患者预后不良的曲线下面积(AUC)分别为0.730、0.748、0.747。ΔCRP联合ΔSFRP5、ΔCRP联合ΔFicolin-3、ΔSFRP5联合ΔFicolin-3、3项指标联合预测Stanford A型ADA患者预后不良的AUC分别为0.810、0.845、0.824、0.925。3项指标联合预测Stanford A型ADA患者预后不良的AUC大于ΔCRP、ΔSFRP5、ΔFicolin-3单独预测的AUC(Z=2.145、2.031、2.047,均P<0.05)。结论手术前后血清CRP、SFRP5、Ficolin-3水平变化值对Stanford A型ADA患者预后不良具有预测价值,联合应用能明显提高预测效能。 展开更多
关键词 Stanford A型主动脉夹层动脉瘤 c反应蛋白 分泌型卷曲相关蛋白5 纤维凝胶蛋白-3 预后
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Relationship Between Leaf C/N Ratio and Insecticidal Protein Expression in Bt Cotton as Affected by High Temperature and N Rate 被引量:9
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作者 ZHANG Xiang LU Chun-hua +3 位作者 CHEN Yuan WANG Gui-xia CHEN Yuan CHEN De-hua 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2014年第1期82-88,共7页
Expression of insecticidal protein for transgenic Bacillus thuringiensis (Bt) cotton is unstable and related to nitrogen metabolism. The objective of this study was to investigate the relationship between leaf carbo... Expression of insecticidal protein for transgenic Bacillus thuringiensis (Bt) cotton is unstable and related to nitrogen metabolism. The objective of this study was to investigate the relationship between leaf carbon nitrogen ratio (C/N) and insecticidal efficacy of two Bt cotton cultivars. C/N ratio and Bt protein content were both measured at peak square period and peak boll period respectively under 5-7 d high temperature and different nitrogen fertilizer rates on the Yangzhou University Farm and the Ludong Cotton Farm, China. All plants were grown in field. The results showed that the C/N ratio enhanced slightly and the Bt protein content remained stable at peak square period, but significant increases for the C/N ratio and decreases markedly for the leaf Bt protein concentration were detected at the peak boll period. The similar patterns at the two growth periods were found for the leaf C/N ratio and Bt protein content by different N fertilizer treatments. When nitrogen rate was from 0 to 600 kg ha-l, the C/N ratio was reduced by 0.017 and 0.006 for Sikang 1 and Sikang 3 at peak square period, compared to the 1.350 to 1.143 reduction for Sikang 1 and Sikang 3 at peak boll period, respectively. Correspondingly, the leaf Bt protein contents were bolstered by 2.6-11.8 and 26.9-36.9% at the two different growth periods, respectively. The results suggested that enhanced C/N ratio by high temperature and nitrogen application may result in the reduction of inseetiocidal efficacy in Bt cotton, especially in peak boll period. 展开更多
关键词 Bt cotton c/n ratio Bt protein insecticidal efficacy
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BIOGEOGRAPHIC RELATIONS OF THE JURASSIC PECTINOIDA AND OSTREOIDA (BIVALVIA) BETWEEN TANGGULA AND OTHER AREAS 被引量:4
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作者 Sha Jingeng (Nanjing Institute of Geology and Palaeontology, Academia Sinica, Nanjing 210008, China) 《地学前缘》 EI CAS CSCD 2000年第S1期139-141,共3页
There are abundant Bajocian—Tithonian bivalves in the main ridge of the Tanggula Mountains of northern Qinghai—Xizang Plateau, China. After figuring the common and coeval species between Tanggula and other areas (Fi... There are abundant Bajocian—Tithonian bivalves in the main ridge of the Tanggula Mountains of northern Qinghai—Xizang Plateau, China. After figuring the common and coeval species between Tanggula and other areas (Fig. 1), and tracing the temporal and spacial historical distribution of the bivalves very capable of dispersion, some Jurassic bivalve biogeographic items, particularly the biogeographic relations, have been lit up. In the known 21 taxa of the Jurassic Pectinoida and Ostreoida from the main ridge of the Tanggula Mountains, there are 12 (57%) common and coeval species in northern Tethys, 13 (62%) common and coeval species in southern Tethys and 12 (57%) in Kachchh—southern Xizang area. It has demonstrated that there existed intermigration of bivalves between Tanggula and various parts of Tethys. Although there is no coeval species between Tanggula and western Australia, in these two areas there exist 6 (29%) close range common species of which 4 (19%) coevaity existed in western Australia and India Plate. Tanggula fauna is, therefore, still very close to that of western Australia. However, there are 15 (71%) common and 14 (67%) coevality species between the main ridge of the Tanggula Mountains and northwestern Europe, among the common species, 8 (38%) ones have lower limitation (first occurrence) in northwestern Europe but only 2 (10%) taxa are older in the main ridge of the Tanggula Mountains. It is very clear that pectinite and ostrea fauna of Tethys, particularly the main ridge of the Tanggula Mountains and Europe had very close relation and most Tanggula’s or northern Tethyan taxa migrated from northwestern Europe. 展开更多
关键词 biogeographic relation JURASSIc BIVALVIA Tanggula of norther n Qinghai—Xizang plateau TETHYS nORTHWESTERn Eu ROPE Pacific
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Correlation Between CO_2 Efflux and Net Nitrogen Mineralization and Its Response to External C or N Supply in an Alpine Meadow Soil 被引量:10
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作者 SONG Ming-Hua JIANG Jing +1 位作者 XU Xing-Liang SHI Pei-Li 《Pedosphere》 SCIE CAS CSCD 2011年第5期666-675,共10页
In nutrient-limited alpine meadows,nitrogen(N) mineralization is prior to soil microbial immobilization;therefore,increased mineral N supply would be most likely immobilized by soil microbes due to nutrient shortage i... In nutrient-limited alpine meadows,nitrogen(N) mineralization is prior to soil microbial immobilization;therefore,increased mineral N supply would be most likely immobilized by soil microbes due to nutrient shortage in alpine soils.In addition,low temperature in alpine meadows might be one of the primary factors limiting soil organic matter decomposition and thus N mineralization.A laboratory incubation experiment was performed using an alpine meadow soil from the Tibetan Plateau.Two levels of NH4NO3(N) or glucose(C) were added,with a blank without addition of C or N as the control,before incubation at 5,15,or 25 ℃ for 28 d.CO2 efflux was measured during the 28-d incubation,and the mineral N was measured at the beginning and end of the incubation,in order to test two hypotheses:1) net N mineralization is negatively correlated with CO2 efflux for the control and 2) the external labile N or C supply will shift the negative correlation to positive.The results showed a negative correlation between CO2 efflux and net N immobilization in the control.External inorganic N supply did not change the negative correlation.The external labile C supply shifted the linear correlation from negative to positive under the low C addition level.However,under the high C level,no correlation was found.These suggested that the correlation of CO2 efflux to net N mineralization strongly depend on soil labile C and C:N ratio regardless of temperatures.Further research should focus on the effects of the types and the amount of litter components on interactions of C and N during soil organic matter decomposition. 展开更多
关键词 cn ratio inorganic n labile c organic matter temperature
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Coupled relationships among anammox,denitrification, and dissimilatory nitrate reduction to ammonium along salinity gradients in a Chinese estuarine wetland 被引量:6
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作者 Zijun Zhou Lei Ge +2 位作者 Yufang Huang Yuqian Liu Siyang Wang 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2021年第8期39-46,共8页
Salinization in estuarine wetlands significantly alters the balance between their nitrogen(N)removal and retention abilities but these processes have not yet been characterized effectively.In the present study,the pot... Salinization in estuarine wetlands significantly alters the balance between their nitrogen(N)removal and retention abilities but these processes have not yet been characterized effectively.In the present study,the potential rates of sediment denitrification,anaerobic ammonium oxidation(anammox),and dissimilatory nitrate reduction to ammonium(DNRA)were mapped using N isotope tracing methods along salinity gradients across the Yellow River Delta wetland(YRDW)in China.The contribution of anammox to total dissimilatory N transformations in YRDW was merely 6.8%,whereas denitrification and DNRA contributed 52.3%and 40.9%,respectively.The potential rate of denitrification(5.82μmol/kg/h)decreased significantly along salinity gradients and markedly exceeded DNRA potential rate(2.7μmol/kg/h)in fresh wetlands,but was lower than that of DNRA in oligohaline wetlands(3.06 and 3.18μmol/kg/h,respectively).Moreover,a significantly positive relationship between salinity and DNRA/denitrification was obeserved,indicating that increased salinity may favor DNRA over denitrification.Furthermore,total sulfur(TS)content and ratio of total organic carbon to total nitrogen(C/N)increased with the salinity gradient and showed evident positive relationships with the DNRA/denitrification ratio.In this study,we proved that increased salinization resulted in the dominance of DNRA over denitrification,possible through the addition of S and alteration of the C/N in estuarine wetlands,leading to increased N retention in estuarine wetlands during salinization,which would enhance the eutrophication potential within wetlands and in downstream ecosystems. 展开更多
关键词 DEnITRIFIcATIOn AnAMMOX DnRA SALInITY c/n ratio
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Relationships Between Abundance of Microbial Functional Genes and the Status and Fluxes of Carbon and Nitrogen in Rice Rhizosphere and Bulk Soils 被引量:5
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作者 NIE San-An XU Hui-Juan +2 位作者 LI Shun LI Hu SU Jian-Qiang 《Pedosphere》 SCIE CAS CSCD 2014年第5期645-651,共7页
Rapid nitrogen(N) transformations and losses occur in the rice rhizosphere through root uptake and microbial activities. However,the relationships between rice roots and rhizosphere microbes for N utilization are stil... Rapid nitrogen(N) transformations and losses occur in the rice rhizosphere through root uptake and microbial activities. However,the relationships between rice roots and rhizosphere microbes for N utilization are still unclear. We analyzed different N forms(NH+4,NO-3, and dissolved organic N), microbial biomass N and C, dissolved organic C, CH4 and N2O emissions, and abundance of microbial functional genes in both rhizosphere and bulk soils after 37-d rice growth in a greenhouse pot experiment. Results showed that the dissolved organic C was significantly higher in the rhizosphere soil than in the non-rhizosphere bulk soil, but microbial biomass C showed no significant difference. The concentrations of NH+4, dissolved organic N, and microbial biomass N in the rhizosphere soil were significantly lower than those of the bulk soil, whereas NO-3in the rhizosphere soil was comparable to that in the bulk soil. The CH4 and N2O fluxes from the rhizosphere soil were much higher than those from the bulk soil. Real-time polymerase chain reaction analysis showed that the abundance of seven selected genes, bacterial and archaeal 16 S rRNA genes, amoA genes of ammonia-oxidizing archaea and ammonia-oxidizing bacteria, nosZ gene, mcrA gene, and pmoA gene, was lower in the rhizosphere soil than in the bulk soil, which is contrary to the results of previous studies. The lower concentration of N in the rhizosphere soil indicated that the competition for N in the rhizosphere soil was very strong, thus having a negative effect on the numbers of microbes. We concluded that when N was limiting, the growth of rhizosphere microorganisms depended on their competitive abilities with rice roots for N. 展开更多
关键词 ammonium-oxidizing archaea ammonia-oxidizing bacteria cH4emission microbial biomass n2O emission organic c organic n
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Variation of Alkaline Phosphatase Activity in Sediments of Shrimp Culture Ponds and Its Relationship with the Contents of C,N and P 被引量:2
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作者 SUYuepeng MAShen DONGShuanglin 《Journal of Ocean University of China》 SCIE CAS 2005年第1期75-79,共5页
Nine enclosures (5 m x 5 m) were built in a Fenneropenaeus chinensis culture pond of Rushan Gulf in April, 2001. The probiotics and BIO ENERGIZER solution were applied for disparate treatments. Variations of alkaline ... Nine enclosures (5 m x 5 m) were built in a Fenneropenaeus chinensis culture pond of Rushan Gulf in April, 2001. The probiotics and BIO ENERGIZER solution were applied for disparate treatments. Variations of alkaline phosphatase ac- tivity (APA) and its relationship with the contents of C, N and P in sediments were studied. Results show that APA of sedi- ments increases from 3.096 nmolg-1min-1 to 5.407 nmol g-1min-1 in culture period; the bacteria biomass is not the only factor to determine APA; the contents of total P and total organic carbon have a significant positive correlation with APA, while that of total nitrogen has a negative correlation. In addition, the contents of inorganic P and organic P are not regular with APA. By comparison, TOC shows a more significant coherence with APA, meaning that organic pollution in sediments af- fects APA remarkably. 展开更多
关键词 APA n c P SEDIMEnTS fenneropenaeus chinensis
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Photosynthetic Light Utilization Efficiency, Water Relations and Leaf Growth of C3 and CAM Tropical Orchids under Natural Conditions 被引量:1
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作者 Shawn Tay Jie He Tim Wing Yam 《American Journal of Plant Sciences》 2015年第18期2949-2959,共11页
Native orchid species of Singapore in their natural conditions experience stress from high irradiance, high temperatures and periods of extended low rainfall, which impact orchid plant physiology and lead to reduced g... Native orchid species of Singapore in their natural conditions experience stress from high irradiance, high temperatures and periods of extended low rainfall, which impact orchid plant physiology and lead to reduced growth and productivity. In this study, it was found that there was a reduction in photochemical efficiency of photosystem II (PSII) in 6 native orchid species under high light (HL) and Bulbophyllum membranaceum under low light (LL). There was chronic photoinhibition in these 6 orchid species over a period of 3 months after transplanting onto the tree trunks without watering and fertilization, especially in Coelogynes mayeriana and Bulbophyllum membranaceum under both HL and LL. This chronic photoinhibition caused by sustained period of water deficit in their natural conditions was later reversed by natural re-watering conditions from higher rainfall. These results indicate that water deficit has a greater impact on photosynthetic light utilization efficiency than excess light. The present study also showed that after natural rewatering, relative water content (RWC) of leaves and pseudobulbs generally increased. During the natural re-watering, total leaf area also gradually increased and reached maximum expansion after 7 weeks under both HL and LL, with some exceptions due to leaf abscission or decline in total leaf area, possibly a strategy for water conservation. 展开更多
关键词 ORcHID cAM c3 PHOTOSYnTHETIc LIGHT Utilization Efficiency Water relations and LEAF Growth
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A GENERAL CONSTITUTIVE RELATION FOR FATIGUE CRACK GROWTH ANALYSIS OF METAL STRUCTURES 被引量:29
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作者 W.C. Cui and X.P. HuangSchool of Naval Architecture & Ocean Engineering, Shanghai Jiaotong University, Shanghai 200030, China 《Acta Metallurgica Sinica(English Letters)》 SCIE EI CAS CSCD 2003年第5期342-354,共13页
Crack growth rate curves are the fundamental material property for metal structures under fatigue loading. Although there are many crack growth rate curves available in the literature, few of them showed the capabilit... Crack growth rate curves are the fundamental material property for metal structures under fatigue loading. Although there are many crack growth rate curves available in the literature, few of them showed the capability to explain various special phenomena observed in tests. A modified constitutive relation recently proposed by McEvily and his co-workers showed very promising capability. This modified constitutive relation is further generalized by (1) introducing an unstable fracture condition; (2) defining a virtual strength to replace the yield stress; and (3) defining an overload and underload parameter. The performances of this general constitutive relation for fatigue crack growth is extensively studied and it is found that this general constitutive relation is able to explain various phenomena observed with particular strong capability on load sequence effect. 展开更多
关键词 metal fatigue S-n curve crack growth rate general constitutive relation mean stress effect load sequence effect
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THE RELATIONSHIP BETWEEN EXPRESSIONS OF N-myc AND c-myc ONCOGENES IN NEUROBLASTOMA:AN IN SITU HYBRIDIZATION AND IMMUNOCYTOCHEMICAL STUDY
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作者 折晓宁 陈杰 +3 位作者 刘彤华 张雷 李佩娟 王德田 《Chinese Medical Sciences Journal》 CAS CSCD 1999年第2期102-106,共5页
INTRODUCTIONNeuroblastomaoriginatesfromthesympathoad-renallineageoftheneuralcrestanditisthemostcommonextra-c... INTRODUCTIONNeuroblastomaoriginatesfromthesympathoad-renallineageoftheneuralcrestanditisthemostcommonextra-cranialtumorofchil... 展开更多
关键词 nEUROBLASTOMA n myc c myc
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General calculation formulas and recurrence relations of radial matrix elements for relativistic n-dimensional hydrogen atom of spin S=0 被引量:1
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作者 CHENChang-yuan LUFa-lin SUNDong-sheng 《原子与分子物理学报》 CAS CSCD 北大核心 2004年第3期432-440,共9页
In this paper, the general calculation formulas of radial matrix elements for relativistic n-dimensional hydrogen atom of spin S=0 are obtained, and the recurrence relation of different power order radial matrix eleme... In this paper, the general calculation formulas of radial matrix elements for relativistic n-dimensional hydrogen atom of spin S=0 are obtained, and the recurrence relation of different power order radial matrix elements are also derived. 展开更多
关键词 n-dimensional hydrogen atom-type potential Klein-cordon equation Radial matrix dements GEnERAL calculation FORMULAS REcURREncE relations
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新型倒置A^(2)/O耦联MBR组合工艺处理农村低C/N废水的研究 被引量:1
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作者 韩卫萍 盖磊 《水处理技术》 CAS 北大核心 2025年第1期114-119,共6页
针对农村低C/N污水污染物和营养盐去除率差的问题,以倒置A^(2)/O耦联膜生物反应器(MBR)组合工艺为探究对象,通过控制进水污染物浓度,在中温条件下考察了有机负荷(OLR)对倒置A^(2)/O耦联MBR组合工艺处理农村低C/N污水的影响。结果表明,OL... 针对农村低C/N污水污染物和营养盐去除率差的问题,以倒置A^(2)/O耦联膜生物反应器(MBR)组合工艺为探究对象,通过控制进水污染物浓度,在中温条件下考察了有机负荷(OLR)对倒置A^(2)/O耦联MBR组合工艺处理农村低C/N污水的影响。结果表明,OLR由150 mg/L提高至450 mg/L时,总氮(TN)和溶解性磷酸盐(SOP)去除率分别由67.6%和86.6%提高至72.4%和94.3%,进一步提高OLR降低了组合工艺对污染物和营养盐的去除。此外,OLR能影响新工艺内污泥特征,提高OLR促进了胞外聚合物(EPS)分泌,尤其在OLR为600 mg/L组别内,EPS含量提高至139.6 mg/g。进水OLR对缺氧池内EPS的影响要大于其对厌氧池内EPS的影响。OLR能影响新工艺内污染物和营养盐去除相关关键酶的活性,当OLR为450 mg/L时,污染物和营养盐去除相关关键活性酶最强。研究结果为农村低C/N污水的高效处理提供了理论依据和数据支撑。 展开更多
关键词 农村低c/n污水 倒置A^(2)/O MBR 胞外聚合物 关键酶
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Field Soil Respiration Rate on a Sub-Antarctic Island: Its Relation to Site Characteristics and Response to Added C, N and P
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作者 Andrea Lubbe Valdon R. Smith 《Open Journal of Soil Science》 2012年第2期187-195,共9页
Botanical, soil chemistry and soil microbiology variables were tested as predictors of in situ soil respiration rate in the various terrestrial habitats on sub-Antarctic Marion Island (47oS, 38oE). Inorganic P and tot... Botanical, soil chemistry and soil microbiology variables were tested as predictors of in situ soil respiration rate in the various terrestrial habitats on sub-Antarctic Marion Island (47oS, 38oE). Inorganic P and total N concentration were the best predictors amongst the chemistry variables and bacteria plate count the best of the microbiology variables. However, while these chemistry and microbiology variables could accurately predict soil respiration rate for particular habitats, they proved inadequate predictors across the whole range of habitats. The best suite of predictors comprised only botanical variables (relative covers of five plant guilds) and accounted for 94% of the total across-habitat variation in soil respiration rate. Mean field soil respiration rates (2.1 - 15.5 mmol CO2 m-2 h-1) for habitats not influenced by seabirds or seals are similar to rates in comparable Northern Hemisphere tundra habitats. Seabird and seal manuring enhances soil respiration rates to values (up to 27.6 mmol CO2 m-2 h-1) higher than found at any tundra site. Glucose, N, P or N plus P were added to three habitats with contrasting soil types;a fellfield with mineral, nutrient-poor soil, a mire with organic, nutrient-poor soil and a shore-zone herbfield heavily manured by penguins and with organic, nutrient-rich soil. Glucose addition stimulated soil respiration in the fellfield and mire (especially the former) but not in the coastal herbfield soil. N and P, alone or together, did not stimulate respiration at any of the habitats, but adding glucose to fellfield soils that had previously been fortified with P or NP caused a similar increase in respiration rate, which was greater than the increase when adding glucose to soils fortified only with N. This suggests that fellfield soil respiration is limited by P rather than N, and that there is no synergism between the two nutrients. For the mire and coastal herbfield, adding glucose to soils previously fortified with N, P or NP did not enhance rates more than adding glucose to soils that had received no nutrient pre-treatment. 展开更多
关键词 SOIL Respiration SUB-AnTARcTIc ISLAnD SOIL Moisture content SOIL nutrient Status n LIMITATIOn P LIMITATIOn c LIMITATIOn Seal and SEABIRD Manuring
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