利用NCBI提供的RefSeq序列,通过BLAT、Sim4和自主开发的剪接比对程序Ealter1.0对人类RefSeq转录本进行外显子预测,根据预测的外显子信息,采用自主开发SYBR Green I Real Time PCR引物设计程序E-qPCR-Design1.0高通量设计21,118对SYBR Gr...利用NCBI提供的RefSeq序列,通过BLAT、Sim4和自主开发的剪接比对程序Ealter1.0对人类RefSeq转录本进行外显子预测,根据预测的外显子信息,采用自主开发SYBR Green I Real Time PCR引物设计程序E-qPCR-Design1.0高通量设计21,118对SYBR Green I Real Time PCR引物,同时选取5000条基因进行SYBR Green I Real Time PCR引物验证,95.92%的基因引物取得良好效果,1.64%的基因引物产生引物二聚体,1.08%的基因引物有非特异性扩增,通过生物信息技术分析与实验验证,建立了基于RefSeq的人类基因荧光定量PCR引物库。展开更多
The Genome Warehouse(GWH),accessible at https://ngdc.cncb.ac.cn/gwh,is an extensively-utilized public repository dedicated to the deposition,management,and sharing of genome assembly sequences,annotations,and metadata...The Genome Warehouse(GWH),accessible at https://ngdc.cncb.ac.cn/gwh,is an extensively-utilized public repository dedicated to the deposition,management,and sharing of genome assembly sequences,annotations,and metadata.This paper highlights noteworthy enhancements to the GWH since the 2021 version,emphasizing substantial advancements in web interfaces for data submission,database functionality updates,and re-source integration.Key updates include the reannotation of released prokaryotic genomes,mirroring of genome resources from National Center for Biotechnology Information(NCBl)GenBank and Reference Sequence Database(RefSeq),integration of Poxviridae sequences,implementation of an online batch submission system,enhancements to the quality control system,advanced search capabilities,and the introduction of a controlled-access mechanism for human genome data.These improvements collectively enhance the ease and security of data submission and access as well as genome data value,thereby improving convenience and utility for researchers in the genomics field.展开更多
文摘利用NCBI提供的RefSeq序列,通过BLAT、Sim4和自主开发的剪接比对程序Ealter1.0对人类RefSeq转录本进行外显子预测,根据预测的外显子信息,采用自主开发SYBR Green I Real Time PCR引物设计程序E-qPCR-Design1.0高通量设计21,118对SYBR Green I Real Time PCR引物,同时选取5000条基因进行SYBR Green I Real Time PCR引物验证,95.92%的基因引物取得良好效果,1.64%的基因引物产生引物二聚体,1.08%的基因引物有非特异性扩增,通过生物信息技术分析与实验验证,建立了基于RefSeq的人类基因荧光定量PCR引物库。
基金supported by the Science&Technology Fundamental Resources Investigation Program(Grant No.2022FY101203 to Xuetong Zhao)the International Partnership Program of the Chinese Academy of Sciences(Grant No.161GJHZ2022002MI to Yiming Bao)+2 种基金the Professional Association of the Alliance of International Science Organizations(Grant No.ANSO-PA-2023-07 to Yiming Bao)the CAS Key Technology Talent Program to Meili Chen,the Open Biodiversity and Health Big Data Programme of IUBS to Yiming Bao,the National Key R&D Program of China(Grant No.2023YFC2605700 to Wenming Zhao)the National Natural Science Foundation of China(Grant Nos.32170678 to Wenming Zhao,32170669 to Jingfa Xiao,and 32030021 to Zhang Zhang).
文摘The Genome Warehouse(GWH),accessible at https://ngdc.cncb.ac.cn/gwh,is an extensively-utilized public repository dedicated to the deposition,management,and sharing of genome assembly sequences,annotations,and metadata.This paper highlights noteworthy enhancements to the GWH since the 2021 version,emphasizing substantial advancements in web interfaces for data submission,database functionality updates,and re-source integration.Key updates include the reannotation of released prokaryotic genomes,mirroring of genome resources from National Center for Biotechnology Information(NCBl)GenBank and Reference Sequence Database(RefSeq),integration of Poxviridae sequences,implementation of an online batch submission system,enhancements to the quality control system,advanced search capabilities,and the introduction of a controlled-access mechanism for human genome data.These improvements collectively enhance the ease and security of data submission and access as well as genome data value,thereby improving convenience and utility for researchers in the genomics field.
