[ Objective] This study aimed to establish an effective method for evaluating uncertainty in determination of reducing sugar content in sugarcane with Lane-Ernon method. [ Method] Based on analysis of the main sources...[ Objective] This study aimed to establish an effective method for evaluating uncertainty in determination of reducing sugar content in sugarcane with Lane-Ernon method. [ Method] Based on analysis of the main sources of uncertainty in determination of reducing sugar content in sugarcane and mathematical model construction, combined uncertainty and expanded uncertainty were determined to establish the method for evaluation of uncertainty in determination. [ Result] Among uncertainties in determination of reducing sugar content in sugarcane with Lane-Ernon method, the greatest uncertainty was introduced by duplicate determination. According to results of statistical analysis, the expanded uncertainty in determination of reducing sugar content was 0.012%. [ Conclusion] This study provided theo- retical reference for evaluation of uncertainty in determination of reducing sugar content in sugarcane.展开更多
[Objective] The aim was to investigate the effects of plant growth regulators on contents of reducing sugar and starch in potato（Solanum tuberosum L.）.[Method]A potato cultivar named ＇Holand-212＇ with three treatm...[Objective] The aim was to investigate the effects of plant growth regulators on contents of reducing sugar and starch in potato（Solanum tuberosum L.）.[Method]A potato cultivar named ＇Holand-212＇ with three treatments by spraying Diethyl aminoethyl hexanoate（DTA-6）,Uniconazole（S3307）and SOD mimics（SODM）were employed to compare differences of the contents of reducing sugar and starch in potato leaves,stolons and tubers in a field experiment.[Result]DTA-6 treatment could significantly reduce the contents of starch and reducing sugar in potato stolons in the period of 100-110 d after seeding,and it could also reduce the content of reducing sugar in tubers,but there is an opposite result in leaves in the same period,significantly.S3307 could extremely significantly increase the content of starch,and can promote the transfer of starch in stolons,in addition,it also could reduce the content of reducing sugar in tubers,and extremely significantly increase the content of starch in leaves at the 110 d after sowing.Moreover,there is a similar effect between SODM and S3307.[Conclusion]The research results are significant for understanding mechanisms of plant growth regulators and promoting application of plant growth regulator in potato.展开更多
The cellulase expands the use of waste lignocellulosic and improves the feasibility of ethanol production with waste lignocellulosic. In this paper the types, mechanisms of cellulase and its application in reducing su...The cellulase expands the use of waste lignocellulosic and improves the feasibility of ethanol production with waste lignocellulosic. In this paper the types, mechanisms of cellulase and its application in reducing sugar production were presented in detail. The strains that produce cellulase and methods for improving the cellulase activity in reducing sugar production with waste lignocellulosic were described.展开更多
Lignocellulosic biomass, being treated only with ammonia, treated with ammonia and then autoclaved, and treated with white-rot fungi after being mechanically chipped, were carried out to access the effects of lignocel...Lignocellulosic biomass, being treated only with ammonia, treated with ammonia and then autoclaved, and treated with white-rot fungi after being mechanically chipped, were carried out to access the effects of lignocellulose degradation by white-rot fimgi cultured on rapeseed straw. Fourier transform infrared spectroscopic analysis was used to show that the white-rot fungus Bjerkandera sp. strain increase the susceptibility of straw to enzymatic saccharification by modifying the lignin component, revealing the effect of these pretreatments on enzymatic saccharification. Reducing sugar production from straws pretreated by ammonia/mechanical chipping/fungi degradation was 29.80% higher than the samples treated with ammonia/autoclaving, indicating an effective degradation of phenolic compounds. After ammonia pretreatment 41% of the straw was converted to RS （reducing sugars） （glucose 50%）. After 5 weeks pretreatment with the white-rot fungus, 54.8% of rapeseed straw was further converted to RS, 74% of which was glucose; while only 12% of the control straw was converted （glucose 42%）. The white-rot fungus Bjerkandera sp. strain degraded rapeseed straw preferentially at the early stage （before 20 d）, and the degradation selectivity was 0.181 1 （cellulose）, 0.364 1 （hemicellulose）, and 0.454 8 （lignin）, suggesting that removal of the phenolic barriers enhanced reducing sugar yield, and the efficiency of fungal pretreatment was comparable with that after alkali treatment, resulting a higher proportion of glucose in the hydrolysates.展开更多
To elucidate the impact of oxygen concentration on the browning reactions containing sugar alcohols and amino acids,the sorbitol-glycine model system was investigated.The color changes under varying oxygen levels were...To elucidate the impact of oxygen concentration on the browning reactions containing sugar alcohols and amino acids,the sorbitol-glycine model system was investigated.The color changes under varying oxygen levels were observed.The melanoidin formation and evolution of browning precursors including reducing sugar,Amadori rearrangement product(ARP),andα-dicarbonyl compounds were traced during the accelerated incubation process at 60℃.Compared with the system under 2%O2,the browning index decreased by about 2.5 times after 28-day storage at 30%O2,with the accumulation rate of fluorescent compounds decreasing by 3.9 times.Furthermore,the content of pyrazine compounds decreased by 20.7 times after six months of storage at 30%O2.This was related to the competition between oxidation reactions and dehydration reactions.Although a higher oxygen concentration promoted the conversion of sorbitol to glucose and the formation of ARP and 3-deoxyglucosone,it also accelerated the oxidative cleavage ofα-dicarbonyl to organic acids and inhibited the retroaldolization of deoxyglucosones into shorter dicarbonyl compounds.Moreover,the inhibitory effect of oxygen on the generation of dicarbonyl compounds was more pronounced,ultimately leading to the suppression of browning.This study proposes a new strategy for maintaining the quality of low-sugar beverages containing sorbitol through modified atmosphere packaging.展开更多
The parameter identification model of largr scale chromatography separation process is proposed. The Phase equilibrium constants and lumped mass transfer coefficients of sugar and reducing sugar adsorption on D1, D2 a...The parameter identification model of largr scale chromatography separation process is proposed. The Phase equilibrium constants and lumped mass transfer coefficients of sugar and reducing sugar adsorption on D1, D2 and D3 resins as well as the axial dispersion coefficients of the fluid through packed columns are determined by means of the pulse-response experiment technique with an inert substance as a tracer and the chromatography measuring technique. The elution curve calculated from these parameters is good agreement with the experimental elution curve. The sensitivity analysis of these parameters is carried out, and the ressult shows that the elation curves of chromatography separation are more sensitive to the variations Of the Phase equilibrium relationship than to the variation of the axial dispersion as well as the lumped mass transfer coefficients.展开更多
This paper studies the effect of reducing sugar concentration, reducing sugar yield and byproducts furfural concentration that generate by straw under the condition of acid concentration and flow rate in the gradient ...This paper studies the effect of reducing sugar concentration, reducing sugar yield and byproducts furfural concentration that generate by straw under the condition of acid concentration and flow rate in the gradient temperature hydrolysis process （60℃-230℃）, we can conclude that reducing sugar yield is highest when the concentration of sulfuric acid is 1%, the velocity of reaction liquid is 25mL/min, at the highest concentration of wheat straw yield of reducing sugar up to 32.9g/L, the reducing sugar yield was 60.8%. At this point the furfural concentration is lower than 1.0g/L, but in the entire gradient heating process, reducing sugar concentration peak of furfural concentration peak appears in the temperature lower, can realize the separation of the reducing sugar and furfural good, reduce the inhibition of fermentation by- products such follow-up treatment process.展开更多
The biorefinery process for sugarcane bagasse saccharification generally requires signifcant accessibility of cellulose. We reported a novel method of cascade cellulase enzymatic hydrol- ysis coupling with ultrafine g...The biorefinery process for sugarcane bagasse saccharification generally requires signifcant accessibility of cellulose. We reported a novel method of cascade cellulase enzymatic hydrol- ysis coupling with ultrafine grinding pretreatment for sugarcane bagasse saccharification. Three enzymatic hydrolysis modes including single cellulase enzymatic hydrolysis, mixed cellulase enzymatic hydrolysis, and cascade cellulase enzymatic hydrolysis were compared. The changes on the functional group and surface morphology of bagasse during cascade cellulase enzymatic hydrolysis were also examined by FT-IR and SEM respectively. The results showed that cascade enzymatic hydrolysis was the most efficient way to enhance the sugarcane bagasse saccharification. More than 65% of reducing sugar yield with 90.1% of glucose selectivity was achieved at 50 ℃, pH=4.8 for 72 h （1200 r/min） with cellulase I of 7.5 FPU/g substrate and cellulase II of 5 FPU/g substrate.展开更多
Sugar cane juice is a popular refreshing drink in most part of Bangladesh. It has great taste and health benefits;also it is available most of the public places at reasonable prices which consumed by road side custome...Sugar cane juice is a popular refreshing drink in most part of Bangladesh. It has great taste and health benefits;also it is available most of the public places at reasonable prices which consumed by road side customers including general public, shopping personals, tourists, students. In our country, street vendors crushing sugarcane between roller crusher and sold without any heat treatment or preservative, also served with or without added ice and lemon juice. The study aimed to identify and compare the physico-chemical and microbial quality of sugarcane juice. The chemical qualities of juices including moisture, P<sup>H</sup>, ash, total soluble solid, total sugar, reducing sugar and titrable acidity were found slightly different in Mymensingh, Gazipur and Narayangonj areas. The highest and lowest value of moisture, ash, P<sup>H</sup>, total soluble solids, total sugar, reducing sugar and titrable acidity were found in 84.33% - 79.26% (Gazipur-Mymensingh), 0.57% - 0.04% (Mymensingh-Gazipur), 5.9 - 2.9 (Gazipur-Narayangonj), 17.48% - 4.98% (Mymensingh-Narayangonj), 21.9% - 6.56% (Mymensingh-Gazipur), 3.7 - 2.1 (Gazipur-Mymensingh) and 0.523% - 0.007% (Narayangonj-Gazipur) respectively. For microbiological analysis, the total viable count of sugar cane juice in Mymensingh, Gazipur and Narayangonj were ranged from 0.6 × 10<sup>5</sup> - 43.6 × 10<sup>5</sup> cfu/ml, 4.6 × 10<sup>5</sup> - 21.6 × 10<sup>5</sup> cfu/ml and 3.6 × 10<sup>5</sup> - 36.6 × 10<sup>5</sup> cfu/ml respectively, where the permitted value is 1.0 × 10<sup>4</sup> cfu/ml, whereas the total coliform count was ranged from 0.4 × 10<sup>5</sup> - 6.4 × 10<sup>5</sup> cfu/ml, 0.6 × 10<sup>5</sup> - 8.4 × 10<sup>5</sup> cfu/ml and 0.00 - 8.4 × 10<sup>5</sup> cfu/ml, where the permitted value is 100 cfu/ml and total fungal count was ranged from 5.5 × 10<sup>5</sup> - 56.5 × 10<sup>5</sup> cfu/ml, 21.5 × 10<sup>5</sup> - 54.5 × 10<sup>5</sup> cfu/ml and 32.5 × 10<sup>5</sup> - 68.5 × 10<sup>5</sup> cfu/ml, where the permitted value is 1000 cfu/ml. According to the Gulf standard, the microbiological parameters of all the collected sugar cane juice were out of the permitted standards, so that a serious health outbreak can be caused anytime.展开更多
[Objective]To explore an effective method for evaluating drought resistance of Yixing lily and provide a reference for selecting drought-resistant Yixing lily varieties. [Method]Through artificial simulation of drough...[Objective]To explore an effective method for evaluating drought resistance of Yixing lily and provide a reference for selecting drought-resistant Yixing lily varieties. [Method]Through artificial simulation of drought stress environment,the changes of physiological indexes,such as content of proline,malonaldehyde,and reducing sugar in Yixing lily leaf and bulb were observed,when soil water content changed. [Result]The response to drought stress of the Yixing lily leaf was greater than that of the bulb,and the content of the proline and malonaldehyde was higher than that of the reducing sugar. [Conclusion]The leaves as an appropriate material for evaluation and analysis on drought resistance can better reflect the physiological responses of Yixing lily to drought stress. And the content of the malonaldehyde and that of proline are preferable indexes.展开更多
Objective] The purpose of this study was to evaluate the effects of five variables （steaming time, moisture content before inoculation, inoculation amount, fer-mentation temperature, fermentation time） on the sweetn...Objective] The purpose of this study was to evaluate the effects of five variables （steaming time, moisture content before inoculation, inoculation amount, fer-mentation temperature, fermentation time） on the sweetness of sweet oats, obtained the best fermentation conditions. [Method] Plackett-Burman （PB） was to evaluate the effects of five variables, and selected significant factors. The steepest ascent was used to approach the optimal response surface experimental area. The optimal fer-mentation condition was obtained by central composite design and response surface analysis. [Results] It was indicated that moisture content, inoculation amount and fer-mentation temperature had significant influences on the content of the reducing sugars. The optimal conditions of moisture content, inoculation amount and temper-ature were 45.26%, 0.014%（g/g） and 28 ℃, respectively. The predicted value of the reducing sugar content was 13.16 mg/g. [Conclusion] Under the optimal conditions, the content of the reducing sugars in the sweet oats could be up to 12.91 mg/g, which was on the whole consistent with the predictive maximum value.展开更多
In adopting tissue culture, effects of rare earth on Physiological and chemical characteristics, proliferation and rootage in aloe were studied. The results indicate that suitable concentration treatment of rare earth...In adopting tissue culture, effects of rare earth on Physiological and chemical characteristics, proliferation and rootage in aloe were studied. The results indicate that suitable concentration treatment of rare earth increases content of reducing sugar and protein, enhances activity intensity of superoxide dismutase (SOD), peroxidase (POD) isozymes and stripes of POD isozymes in the leaves. It also increases rate of proliferation and rootage respectively. The appropriate concentration of rare earth is 20～40 mg·L -1 in proliferation and rootage of aloe.展开更多
Cherry cultivar Misri （Bigarreau Noir Grossa） was used for making candy. Pitting of fruit was done manually and KMS （0.2%） was added for bleaching. The fruit was pretreated using 4 levels of CaCl2 （Co = 0%, C1 = ...Cherry cultivar Misri （Bigarreau Noir Grossa） was used for making candy. Pitting of fruit was done manually and KMS （0.2%） was added for bleaching. The fruit was pretreated using 4 levels of CaCl2 （Co = 0%, C1 = 1%, C2 = 1.5%, C3 = 2%） and stored in jars for 4 weeks. Thereafter, application of colour was done using Erythrosine （0.05%） followed by dipping pitted fruits in sugar syrup for 24 hours. The samples were treated with 4 levels of citric acid （To = 0%, Tl = 1%, T2 = 1.5%, T3 = 2%） and then concentrated subsequently till desired TSS of 70~ Brix was attained. The samples were dried in cabinet drier at 80 ±20 ℃till constant moisture was attained. The product was packed in polyethene pouches and stored under ambient storage conditions. Results revealed that there was gradual increase in reducing sugars （%）, total sugars （%） during storage while as moisture content （%） decreased. The product developed was found economically profitable and viable for commercial production.展开更多
The hydrolysis process to obtain the so-called ＂reducing sugars＂ represents the main step involved in the production of the second generation bioethanol. This product can be obtained directly from various types of g...The hydrolysis process to obtain the so-called ＂reducing sugars＂ represents the main step involved in the production of the second generation bioethanol. This product can be obtained directly from various types of green biomass, replacing the use of cereals cultivations, with obvious benefits to the environment and the economy of agricultural production. However, it is necessary to improve the hydrolysis process of the cellulose to achieve this goal. To this purpose, we applied a chemical process formerly used. The values of sugars yield were increased by about 40% with respect to the previous study. Further significant cost savings were accomplished, resulting from the recovery of the by-product, calcium sulfate, commercially known as gypsum.展开更多
Aloe vera (Aloe barbadensis Miller) possesses curative and therapeutic properties attributed to the polysaccharides found in its tissue. This work aimed to study hydrolyzed (chemical and enzymatical) Aloe vera gel ext...Aloe vera (Aloe barbadensis Miller) possesses curative and therapeutic properties attributed to the polysaccharides found in its tissue. This work aimed to study hydrolyzed (chemical and enzymatical) Aloe vera gel extracts using ultrafiltration membranes with a pore size of 0.22 micron (μm). Nine chemical treatments were achieved with H2SO4 and three wet heat treatments were carried out at a pressure of 1.2 lbf/in2 for 15 minutes;in both cases, three different concentrations of Aloe vera gel juice (AGJ) were used: 1.5%, 2.5% and 3.5% w/w. The concentrations of H2SO4 were 0.10%, 0.25% and 0.40% w/w. Chemical experiments are performed over a factorial 32 design and results were analyzed using SPSS software (version 17, SPSS Inc.), finding the one labeled T7 (15 ml of AGJ 3.5% with 15 ml of H2SO4 0.10% added) the best of them, as it leaded to 0.0446 mg/ml of liberated glucose. Among the three wet heat treatments, the one labeled TC3 (15 ml of AGJ 3.5% with 15 ml of H2O added) was the best-performing one, as it leaded to 0.292 mg/ml of liberated glucose. Furthermore, an enzymatic hydrolysis was carried out using Novozymes’ Pectinex? AR and Viscozyme?. Hydrolisis with both enzymes yield to better results than acid hydrolysis: in the treatment with Pectinex? AR, 3.282 mg/ml of liberated glucose were obtained and 3.302 mg/ml in the treatment with Viscozyme?. The hydrolyzed substances obtained by acid and enzymatic hydrolysis, as well as by wet heat treatment, were subsequently analyzed by thin layer chromatography (TLC), using glucose, galactose and arabinose 1000 ppm solutions as reference patterns. Among the treatments by H2SO4, the one labeled T4 obtained an Rf value of 50, the same as on the galactose reference pattern.展开更多
Objective: to investigate the clinical effect of quench thirst and Jiangtang prescription on stage ⅲ diabetic nephropathy by observing the clinical effect of quench thirst and Jiangtang prescription on stage ⅲ diabe...Objective: to investigate the clinical effect of quench thirst and Jiangtang prescription on stage ⅲ diabetic nephropathy by observing the clinical effect of quench thirst and Jiangtang prescription on stage ⅲ diabetic nephropathy patients with deficiency of spleen and kidney and blood stasis blocking collaterals. Methods: 60 cases of diabetic nephropathy diagnosed in the First Affiliated Hospital of Traditional Chinese Medicine of Guangxi University were randomly divided into treatment group and control group, 30 cases in each group. The control group was given losartan potassium tablets, and the treatment group was given cough relieving glucose-lowering prescription on this basis. The changes of CHOL, TG, LDL-C, CFR, BUN, SCR and 24-hour urinary protein were detected at the first, second and third month, respectively. Results: the index of cholesterol, triglyceride and low density lipoprotein cholesterol decreased in the treatment group after treatment (P < 0.05), but not in the control group before and after treatment (P > 0.05). Conclusion: it can effectively relieve clinical symptoms, regulate blood glucose, improve abnormal lipid metabolism, and improve glomerular filtration rate, urinary microalbumin and other indicators of renal function, and delay the deterioration of renal function.展开更多
The characteristic flavor of the enzymatic hydrolysate of Lentinus edodes,which was involved in the Maillard reaction by xylose,fructose,glucose,sucrose,mannose,maltose,vitamin C(VC)and L-arabinose,was determined and ...The characteristic flavor of the enzymatic hydrolysate of Lentinus edodes,which was involved in the Maillard reaction by xylose,fructose,glucose,sucrose,mannose,maltose,vitamin C(VC)and L-arabinose,was determined and analyzed by gas chromatography-ion mobility spectroscopy(GC-IMS).The characteristic flavor fingerprints of Maillard reaction products(MRPs)of L.edodes enzymatic hydrolysate were constructed.The differences in the characteristic flavors of Maillard reaction with different reducing sugars and VC were compared.The main characteristic flavor,characteristic peak,characteristic marker substance and content were determined,a principal component analysis(PCA)of volatile organic compounds(VOCs)was carried out.The results showed that there were 42 kinds of monomers and some dimers of volatile compounds in the samples including 17 aldehydes,5 alcohols,7 ketones,2 esters,2 acids,7 other compounds and 2 ethers.The volatile VOCs of the sample with added sucrose,VC and L-arabinose were significantly higher than those of the enzymatic hydrolysate of L.edodes.After the Maillard reaction,the flavor of the hydrolysate was significantly improved,and the main components changed significantly.展开更多
As one way to eliminate the issues found in the preceding generation,feedstock exploration in second-generation bioethanol production remains an issue,especially for a tropical country such as Indonesia.From exotic fr...As one way to eliminate the issues found in the preceding generation,feedstock exploration in second-generation bioethanol production remains an issue,especially for a tropical country such as Indonesia.From exotic fruit by-products,durian holds a promising perspective that rests on its abundance,superb carbohydrate content and limited usage until now.This work presents the first-ever utilization of durian seeds for sugar production under optimized conditions through alkaline hydrolysis.A simple form of sugar was extracted by varying four parameters,namely substrate loading,NaOH concentration,hydrolysis time and hydrolysis temperature.Response surface methodology based on the Box-Behnken design was employed to outline the most optimum parameter values.Analysis of variance revealed that the quadratic model fit the data appropriately with the order of significance as substrate loading>hydrolysis time>NaOH concentration>hydrolysis temperature.The optimized conditions for reducing sugar yield,as high as 2.140 g/L,corresponded to<50 g/L substrate loading,0.522 M NaOH,60 minutes of hydrolysis time and 80oC hydrolysis temperature.The possible ethanol content of 1.094 g/L was also expected under optimized conditions,demonstrating great potential in second-generation bioethanol production.展开更多
Starch and sugar properties and sweetness of storage root(SR)are vital to the economic value of sweet potato.To clarify the factors determining these properties of raw sweet potato,eleven varieties with different SR p...Starch and sugar properties and sweetness of storage root(SR)are vital to the economic value of sweet potato.To clarify the factors determining these properties of raw sweet potato,eleven varieties with different SR properties were selected to investigate the changes of starch and sugar-related traits,brix and amylase activities during the growth and development of SRs,and the correlation among these SR properties and the amylase activities were analyzed.Fiveβ-amylase genes expressed in SRs were cloned,and their expression and characteristics were analyzed to reveal the involvement of amylase in these SR properties.The results showed total sugar,soluble sugar,reducing sugar content and brix varied greatly among different varieties and development periods,β-amylase activities were much higher than that ofα-amylase in SRs,dynamically changed during the devel-opment of SRs,and decreased at the late stage of SR expansion.The expression of threeβ-amylase encoding genes were positively correlated with the total sugar content,and might play vital roles in starch and sugar metabolism in SRs.The brix of raw SRs was significantly correlated with total starch,amylose,amylopectin,total sugar content and reducing sugar content,but had no significant relationship with soluble sugar content andβ-amylase activity.These results indicated that the brix of SR was determined by both starch and sugar components,and multipleβ-amylases function together in starch and sugar metabolism in developing SRs.These findings provide important information for the improvement of sweetness and the quality of processed products in sweet potato.展开更多
Brown rice bread has received increasing attention due to its health benefits,but the incorporation of brown rice flour usually shows detrimental effects on bread quality.Brown rice pretreatment is an effective way to...Brown rice bread has received increasing attention due to its health benefits,but the incorporation of brown rice flour usually shows detrimental effects on bread quality.Brown rice pretreatment is an effective way to improve bread quality.Thus,the impacts of enzymatic extruded brown rice flour(EEBRF)on wheat-based dough prop-erties and bread quality were investigated,and then the effects of EEBRF were compared with that of brown rice flour(BRF)and extruded brown rice flour(EBRF).Results indicated that EEBRF had significantly higher reducing sugar content and water solubility but significantly lower water absorption than that of BRF and EBRF.Incor-poration of BRF or EBRF increased solid-like behavior of dough,while the dough with EEBRF incorporation showed comparable viscoelasticity to that of wheat dough.Besides,the dough with BRF or EBRF incorporation exhibited significantly higher resistance to extension and lower extensibility,but the dough with EEBRF incor-poration showed the highest resistance to extension and comparable extensibility when compared with that of wheat dough.Furthermore,discontinuous and loose structure was observed in the dough with BRF or EBRF incorporation,whereas compact and continuous structure was found in the dough with EEBRF incorporation.Consequently,the composite bread made with EEBRF showed larger specific volume,more appealing crust color,bigger and thinner cells and softer texture than other composite breads and even wheat bread.The above results demonstrated the great potential of EEBRF in improving bread quality,which could be applicated in the development of functional brown rice bread.展开更多
文摘[ Objective] This study aimed to establish an effective method for evaluating uncertainty in determination of reducing sugar content in sugarcane with Lane-Ernon method. [ Method] Based on analysis of the main sources of uncertainty in determination of reducing sugar content in sugarcane and mathematical model construction, combined uncertainty and expanded uncertainty were determined to establish the method for evaluation of uncertainty in determination. [ Result] Among uncertainties in determination of reducing sugar content in sugarcane with Lane-Ernon method, the greatest uncertainty was introduced by duplicate determination. According to results of statistical analysis, the expanded uncertainty in determination of reducing sugar content was 0.012%. [ Conclusion] This study provided theo- retical reference for evaluation of uncertainty in determination of reducing sugar content in sugarcane.
基金Supported by Daqing Scientific and Technological Project in Heilongjiang Province(SGG2007-054)Demonstration and Promotion Projects of Potato Comprehensive High Yield Cultivation Technologyin Western Regions of Heilongjiang Province(PC07B09)~~
文摘[Objective] The aim was to investigate the effects of plant growth regulators on contents of reducing sugar and starch in potato（Solanum tuberosum L.）.[Method]A potato cultivar named ＇Holand-212＇ with three treatments by spraying Diethyl aminoethyl hexanoate（DTA-6）,Uniconazole（S3307）and SOD mimics（SODM）were employed to compare differences of the contents of reducing sugar and starch in potato leaves,stolons and tubers in a field experiment.[Result]DTA-6 treatment could significantly reduce the contents of starch and reducing sugar in potato stolons in the period of 100-110 d after seeding,and it could also reduce the content of reducing sugar in tubers,but there is an opposite result in leaves in the same period,significantly.S3307 could extremely significantly increase the content of starch,and can promote the transfer of starch in stolons,in addition,it also could reduce the content of reducing sugar in tubers,and extremely significantly increase the content of starch in leaves at the 110 d after sowing.Moreover,there is a similar effect between SODM and S3307.[Conclusion]The research results are significant for understanding mechanisms of plant growth regulators and promoting application of plant growth regulator in potato.
文摘The cellulase expands the use of waste lignocellulosic and improves the feasibility of ethanol production with waste lignocellulosic. In this paper the types, mechanisms of cellulase and its application in reducing sugar production were presented in detail. The strains that produce cellulase and methods for improving the cellulase activity in reducing sugar production with waste lignocellulosic were described.
基金supported by the New & Renewable Energy of the Korea Institute of Energy Technology Evaluation and Planning(KETEP)Grant Funded by the Korea Government Ministry of Knowledge Economy(No.20103020090020)
文摘Lignocellulosic biomass, being treated only with ammonia, treated with ammonia and then autoclaved, and treated with white-rot fungi after being mechanically chipped, were carried out to access the effects of lignocellulose degradation by white-rot fimgi cultured on rapeseed straw. Fourier transform infrared spectroscopic analysis was used to show that the white-rot fungus Bjerkandera sp. strain increase the susceptibility of straw to enzymatic saccharification by modifying the lignin component, revealing the effect of these pretreatments on enzymatic saccharification. Reducing sugar production from straws pretreated by ammonia/mechanical chipping/fungi degradation was 29.80% higher than the samples treated with ammonia/autoclaving, indicating an effective degradation of phenolic compounds. After ammonia pretreatment 41% of the straw was converted to RS （reducing sugars） （glucose 50%）. After 5 weeks pretreatment with the white-rot fungus, 54.8% of rapeseed straw was further converted to RS, 74% of which was glucose; while only 12% of the control straw was converted （glucose 42%）. The white-rot fungus Bjerkandera sp. strain degraded rapeseed straw preferentially at the early stage （before 20 d）, and the degradation selectivity was 0.181 1 （cellulose）, 0.364 1 （hemicellulose）, and 0.454 8 （lignin）, suggesting that removal of the phenolic barriers enhanced reducing sugar yield, and the efficiency of fungal pretreatment was comparable with that after alkali treatment, resulting a higher proportion of glucose in the hydrolysates.
基金support of National Key R&D Program of China(2023YFD2100500)program of“Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province”.
文摘To elucidate the impact of oxygen concentration on the browning reactions containing sugar alcohols and amino acids,the sorbitol-glycine model system was investigated.The color changes under varying oxygen levels were observed.The melanoidin formation and evolution of browning precursors including reducing sugar,Amadori rearrangement product(ARP),andα-dicarbonyl compounds were traced during the accelerated incubation process at 60℃.Compared with the system under 2%O2,the browning index decreased by about 2.5 times after 28-day storage at 30%O2,with the accumulation rate of fluorescent compounds decreasing by 3.9 times.Furthermore,the content of pyrazine compounds decreased by 20.7 times after six months of storage at 30%O2.This was related to the competition between oxidation reactions and dehydration reactions.Although a higher oxygen concentration promoted the conversion of sorbitol to glucose and the formation of ARP and 3-deoxyglucosone,it also accelerated the oxidative cleavage ofα-dicarbonyl to organic acids and inhibited the retroaldolization of deoxyglucosones into shorter dicarbonyl compounds.Moreover,the inhibitory effect of oxygen on the generation of dicarbonyl compounds was more pronounced,ultimately leading to the suppression of browning.This study proposes a new strategy for maintaining the quality of low-sugar beverages containing sorbitol through modified atmosphere packaging.
文摘The parameter identification model of largr scale chromatography separation process is proposed. The Phase equilibrium constants and lumped mass transfer coefficients of sugar and reducing sugar adsorption on D1, D2 and D3 resins as well as the axial dispersion coefficients of the fluid through packed columns are determined by means of the pulse-response experiment technique with an inert substance as a tracer and the chromatography measuring technique. The elution curve calculated from these parameters is good agreement with the experimental elution curve. The sensitivity analysis of these parameters is carried out, and the ressult shows that the elation curves of chromatography separation are more sensitive to the variations Of the Phase equilibrium relationship than to the variation of the axial dispersion as well as the lumped mass transfer coefficients.
文摘This paper studies the effect of reducing sugar concentration, reducing sugar yield and byproducts furfural concentration that generate by straw under the condition of acid concentration and flow rate in the gradient temperature hydrolysis process （60℃-230℃）, we can conclude that reducing sugar yield is highest when the concentration of sulfuric acid is 1%, the velocity of reaction liquid is 25mL/min, at the highest concentration of wheat straw yield of reducing sugar up to 32.9g/L, the reducing sugar yield was 60.8%. At this point the furfural concentration is lower than 1.0g/L, but in the entire gradient heating process, reducing sugar concentration peak of furfural concentration peak appears in the temperature lower, can realize the separation of the reducing sugar and furfural good, reduce the inhibition of fermentation by- products such follow-up treatment process.
基金This work was supported by the National Highteeh R＆D Program of China （No.2012AA101806）, the National Natural Science Foundation of China （No.51306191）, and the National Key Technology R＆D Program （No.2014BAD02B01）.
文摘The biorefinery process for sugarcane bagasse saccharification generally requires signifcant accessibility of cellulose. We reported a novel method of cascade cellulase enzymatic hydrol- ysis coupling with ultrafine grinding pretreatment for sugarcane bagasse saccharification. Three enzymatic hydrolysis modes including single cellulase enzymatic hydrolysis, mixed cellulase enzymatic hydrolysis, and cascade cellulase enzymatic hydrolysis were compared. The changes on the functional group and surface morphology of bagasse during cascade cellulase enzymatic hydrolysis were also examined by FT-IR and SEM respectively. The results showed that cascade enzymatic hydrolysis was the most efficient way to enhance the sugarcane bagasse saccharification. More than 65% of reducing sugar yield with 90.1% of glucose selectivity was achieved at 50 ℃, pH=4.8 for 72 h （1200 r/min） with cellulase I of 7.5 FPU/g substrate and cellulase II of 5 FPU/g substrate.
文摘Sugar cane juice is a popular refreshing drink in most part of Bangladesh. It has great taste and health benefits;also it is available most of the public places at reasonable prices which consumed by road side customers including general public, shopping personals, tourists, students. In our country, street vendors crushing sugarcane between roller crusher and sold without any heat treatment or preservative, also served with or without added ice and lemon juice. The study aimed to identify and compare the physico-chemical and microbial quality of sugarcane juice. The chemical qualities of juices including moisture, P<sup>H</sup>, ash, total soluble solid, total sugar, reducing sugar and titrable acidity were found slightly different in Mymensingh, Gazipur and Narayangonj areas. The highest and lowest value of moisture, ash, P<sup>H</sup>, total soluble solids, total sugar, reducing sugar and titrable acidity were found in 84.33% - 79.26% (Gazipur-Mymensingh), 0.57% - 0.04% (Mymensingh-Gazipur), 5.9 - 2.9 (Gazipur-Narayangonj), 17.48% - 4.98% (Mymensingh-Narayangonj), 21.9% - 6.56% (Mymensingh-Gazipur), 3.7 - 2.1 (Gazipur-Mymensingh) and 0.523% - 0.007% (Narayangonj-Gazipur) respectively. For microbiological analysis, the total viable count of sugar cane juice in Mymensingh, Gazipur and Narayangonj were ranged from 0.6 × 10<sup>5</sup> - 43.6 × 10<sup>5</sup> cfu/ml, 4.6 × 10<sup>5</sup> - 21.6 × 10<sup>5</sup> cfu/ml and 3.6 × 10<sup>5</sup> - 36.6 × 10<sup>5</sup> cfu/ml respectively, where the permitted value is 1.0 × 10<sup>4</sup> cfu/ml, whereas the total coliform count was ranged from 0.4 × 10<sup>5</sup> - 6.4 × 10<sup>5</sup> cfu/ml, 0.6 × 10<sup>5</sup> - 8.4 × 10<sup>5</sup> cfu/ml and 0.00 - 8.4 × 10<sup>5</sup> cfu/ml, where the permitted value is 100 cfu/ml and total fungal count was ranged from 5.5 × 10<sup>5</sup> - 56.5 × 10<sup>5</sup> cfu/ml, 21.5 × 10<sup>5</sup> - 54.5 × 10<sup>5</sup> cfu/ml and 32.5 × 10<sup>5</sup> - 68.5 × 10<sup>5</sup> cfu/ml, where the permitted value is 1000 cfu/ml. According to the Gulf standard, the microbiological parameters of all the collected sugar cane juice were out of the permitted standards, so that a serious health outbreak can be caused anytime.
基金Supported by Natural Science Foundation of Hubei Province(2005ABA211)~~
文摘[Objective]To explore an effective method for evaluating drought resistance of Yixing lily and provide a reference for selecting drought-resistant Yixing lily varieties. [Method]Through artificial simulation of drought stress environment,the changes of physiological indexes,such as content of proline,malonaldehyde,and reducing sugar in Yixing lily leaf and bulb were observed,when soil water content changed. [Result]The response to drought stress of the Yixing lily leaf was greater than that of the bulb,and the content of the proline and malonaldehyde was higher than that of the reducing sugar. [Conclusion]The leaves as an appropriate material for evaluation and analysis on drought resistance can better reflect the physiological responses of Yixing lily to drought stress. And the content of the malonaldehyde and that of proline are preferable indexes.
基金Major science and technology projects in jiangxi province(2010ZX0100)Basic scientific research business expenses Incremental projects of Chinese academy of agricultural sciences(2013ZL013)~~
文摘Objective] The purpose of this study was to evaluate the effects of five variables （steaming time, moisture content before inoculation, inoculation amount, fer-mentation temperature, fermentation time） on the sweetness of sweet oats, obtained the best fermentation conditions. [Method] Plackett-Burman （PB） was to evaluate the effects of five variables, and selected significant factors. The steepest ascent was used to approach the optimal response surface experimental area. The optimal fer-mentation condition was obtained by central composite design and response surface analysis. [Results] It was indicated that moisture content, inoculation amount and fer-mentation temperature had significant influences on the content of the reducing sugars. The optimal conditions of moisture content, inoculation amount and temper-ature were 45.26%, 0.014%（g/g） and 28 ℃, respectively. The predicted value of the reducing sugar content was 13.16 mg/g. [Conclusion] Under the optimal conditions, the content of the reducing sugars in the sweet oats could be up to 12.91 mg/g, which was on the whole consistent with the predictive maximum value.
文摘In adopting tissue culture, effects of rare earth on Physiological and chemical characteristics, proliferation and rootage in aloe were studied. The results indicate that suitable concentration treatment of rare earth increases content of reducing sugar and protein, enhances activity intensity of superoxide dismutase (SOD), peroxidase (POD) isozymes and stripes of POD isozymes in the leaves. It also increases rate of proliferation and rootage respectively. The appropriate concentration of rare earth is 20～40 mg·L -1 in proliferation and rootage of aloe.
文摘Cherry cultivar Misri （Bigarreau Noir Grossa） was used for making candy. Pitting of fruit was done manually and KMS （0.2%） was added for bleaching. The fruit was pretreated using 4 levels of CaCl2 （Co = 0%, C1 = 1%, C2 = 1.5%, C3 = 2%） and stored in jars for 4 weeks. Thereafter, application of colour was done using Erythrosine （0.05%） followed by dipping pitted fruits in sugar syrup for 24 hours. The samples were treated with 4 levels of citric acid （To = 0%, Tl = 1%, T2 = 1.5%, T3 = 2%） and then concentrated subsequently till desired TSS of 70~ Brix was attained. The samples were dried in cabinet drier at 80 ±20 ℃till constant moisture was attained. The product was packed in polyethene pouches and stored under ambient storage conditions. Results revealed that there was gradual increase in reducing sugars （%）, total sugars （%） during storage while as moisture content （%） decreased. The product developed was found economically profitable and viable for commercial production.
文摘The hydrolysis process to obtain the so-called ＂reducing sugars＂ represents the main step involved in the production of the second generation bioethanol. This product can be obtained directly from various types of green biomass, replacing the use of cereals cultivations, with obvious benefits to the environment and the economy of agricultural production. However, it is necessary to improve the hydrolysis process of the cellulose to achieve this goal. To this purpose, we applied a chemical process formerly used. The values of sugars yield were increased by about 40% with respect to the previous study. Further significant cost savings were accomplished, resulting from the recovery of the by-product, calcium sulfate, commercially known as gypsum.
文摘Aloe vera (Aloe barbadensis Miller) possesses curative and therapeutic properties attributed to the polysaccharides found in its tissue. This work aimed to study hydrolyzed (chemical and enzymatical) Aloe vera gel extracts using ultrafiltration membranes with a pore size of 0.22 micron (μm). Nine chemical treatments were achieved with H2SO4 and three wet heat treatments were carried out at a pressure of 1.2 lbf/in2 for 15 minutes;in both cases, three different concentrations of Aloe vera gel juice (AGJ) were used: 1.5%, 2.5% and 3.5% w/w. The concentrations of H2SO4 were 0.10%, 0.25% and 0.40% w/w. Chemical experiments are performed over a factorial 32 design and results were analyzed using SPSS software (version 17, SPSS Inc.), finding the one labeled T7 (15 ml of AGJ 3.5% with 15 ml of H2SO4 0.10% added) the best of them, as it leaded to 0.0446 mg/ml of liberated glucose. Among the three wet heat treatments, the one labeled TC3 (15 ml of AGJ 3.5% with 15 ml of H2O added) was the best-performing one, as it leaded to 0.292 mg/ml of liberated glucose. Furthermore, an enzymatic hydrolysis was carried out using Novozymes’ Pectinex? AR and Viscozyme?. Hydrolisis with both enzymes yield to better results than acid hydrolysis: in the treatment with Pectinex? AR, 3.282 mg/ml of liberated glucose were obtained and 3.302 mg/ml in the treatment with Viscozyme?. The hydrolyzed substances obtained by acid and enzymatic hydrolysis, as well as by wet heat treatment, were subsequently analyzed by thin layer chromatography (TLC), using glucose, galactose and arabinose 1000 ppm solutions as reference patterns. Among the treatments by H2SO4, the one labeled T4 obtained an Rf value of 50, the same as on the galactose reference pattern.
文摘Objective: to investigate the clinical effect of quench thirst and Jiangtang prescription on stage ⅲ diabetic nephropathy by observing the clinical effect of quench thirst and Jiangtang prescription on stage ⅲ diabetic nephropathy patients with deficiency of spleen and kidney and blood stasis blocking collaterals. Methods: 60 cases of diabetic nephropathy diagnosed in the First Affiliated Hospital of Traditional Chinese Medicine of Guangxi University were randomly divided into treatment group and control group, 30 cases in each group. The control group was given losartan potassium tablets, and the treatment group was given cough relieving glucose-lowering prescription on this basis. The changes of CHOL, TG, LDL-C, CFR, BUN, SCR and 24-hour urinary protein were detected at the first, second and third month, respectively. Results: the index of cholesterol, triglyceride and low density lipoprotein cholesterol decreased in the treatment group after treatment (P < 0.05), but not in the control group before and after treatment (P > 0.05). Conclusion: it can effectively relieve clinical symptoms, regulate blood glucose, improve abnormal lipid metabolism, and improve glomerular filtration rate, urinary microalbumin and other indicators of renal function, and delay the deterioration of renal function.
基金This work was supported by the Innovative Engineering project of Shandong Academy of Agricultural Sciences(CXGC2022A36)Modern Agricultural Industry Technology System of Shandong Province(SDAIT-07-09).
文摘The characteristic flavor of the enzymatic hydrolysate of Lentinus edodes,which was involved in the Maillard reaction by xylose,fructose,glucose,sucrose,mannose,maltose,vitamin C(VC)and L-arabinose,was determined and analyzed by gas chromatography-ion mobility spectroscopy(GC-IMS).The characteristic flavor fingerprints of Maillard reaction products(MRPs)of L.edodes enzymatic hydrolysate were constructed.The differences in the characteristic flavors of Maillard reaction with different reducing sugars and VC were compared.The main characteristic flavor,characteristic peak,characteristic marker substance and content were determined,a principal component analysis(PCA)of volatile organic compounds(VOCs)was carried out.The results showed that there were 42 kinds of monomers and some dimers of volatile compounds in the samples including 17 aldehydes,5 alcohols,7 ketones,2 esters,2 acids,7 other compounds and 2 ethers.The volatile VOCs of the sample with added sucrose,VC and L-arabinose were significantly higher than those of the enzymatic hydrolysate of L.edodes.After the Maillard reaction,the flavor of the hydrolysate was significantly improved,and the main components changed significantly.
基金the Centre of Research and Community Services,Sampoerna University,under internal research grant 022/IRG/SU/AY.2019-2020.
文摘As one way to eliminate the issues found in the preceding generation,feedstock exploration in second-generation bioethanol production remains an issue,especially for a tropical country such as Indonesia.From exotic fruit by-products,durian holds a promising perspective that rests on its abundance,superb carbohydrate content and limited usage until now.This work presents the first-ever utilization of durian seeds for sugar production under optimized conditions through alkaline hydrolysis.A simple form of sugar was extracted by varying four parameters,namely substrate loading,NaOH concentration,hydrolysis time and hydrolysis temperature.Response surface methodology based on the Box-Behnken design was employed to outline the most optimum parameter values.Analysis of variance revealed that the quadratic model fit the data appropriately with the order of significance as substrate loading>hydrolysis time>NaOH concentration>hydrolysis temperature.The optimized conditions for reducing sugar yield,as high as 2.140 g/L,corresponded to<50 g/L substrate loading,0.522 M NaOH,60 minutes of hydrolysis time and 80oC hydrolysis temperature.The possible ethanol content of 1.094 g/L was also expected under optimized conditions,demonstrating great potential in second-generation bioethanol production.
基金supported by special fund for youth team of the Southwest Universities(SWU-XJPY202306)the Technology Innovation and Application Development Key Project of Chongqing(cstc2021jscxgksbX0022)+1 种基金the special fund for Chongqing Beibei germplasm bank of tuber and root crops(ZWZZ2020005)the special funding from Chongqing modern agriculture industry technology system innovation team(CQMAITS202303-3).
文摘Starch and sugar properties and sweetness of storage root(SR)are vital to the economic value of sweet potato.To clarify the factors determining these properties of raw sweet potato,eleven varieties with different SR properties were selected to investigate the changes of starch and sugar-related traits,brix and amylase activities during the growth and development of SRs,and the correlation among these SR properties and the amylase activities were analyzed.Fiveβ-amylase genes expressed in SRs were cloned,and their expression and characteristics were analyzed to reveal the involvement of amylase in these SR properties.The results showed total sugar,soluble sugar,reducing sugar content and brix varied greatly among different varieties and development periods,β-amylase activities were much higher than that ofα-amylase in SRs,dynamically changed during the devel-opment of SRs,and decreased at the late stage of SR expansion.The expression of threeβ-amylase encoding genes were positively correlated with the total sugar content,and might play vital roles in starch and sugar metabolism in SRs.The brix of raw SRs was significantly correlated with total starch,amylose,amylopectin,total sugar content and reducing sugar content,but had no significant relationship with soluble sugar content andβ-amylase activity.These results indicated that the brix of SR was determined by both starch and sugar components,and multipleβ-amylases function together in starch and sugar metabolism in developing SRs.These findings provide important information for the improvement of sweetness and the quality of processed products in sweet potato.
基金supported by the National Natural Science Foundation of China(32360600)Jiangxi Provincial Natural Science Foundation(20224BAB215047).
文摘Brown rice bread has received increasing attention due to its health benefits,but the incorporation of brown rice flour usually shows detrimental effects on bread quality.Brown rice pretreatment is an effective way to improve bread quality.Thus,the impacts of enzymatic extruded brown rice flour(EEBRF)on wheat-based dough prop-erties and bread quality were investigated,and then the effects of EEBRF were compared with that of brown rice flour(BRF)and extruded brown rice flour(EBRF).Results indicated that EEBRF had significantly higher reducing sugar content and water solubility but significantly lower water absorption than that of BRF and EBRF.Incor-poration of BRF or EBRF increased solid-like behavior of dough,while the dough with EEBRF incorporation showed comparable viscoelasticity to that of wheat dough.Besides,the dough with BRF or EBRF incorporation exhibited significantly higher resistance to extension and lower extensibility,but the dough with EEBRF incor-poration showed the highest resistance to extension and comparable extensibility when compared with that of wheat dough.Furthermore,discontinuous and loose structure was observed in the dough with BRF or EBRF incorporation,whereas compact and continuous structure was found in the dough with EEBRF incorporation.Consequently,the composite bread made with EEBRF showed larger specific volume,more appealing crust color,bigger and thinner cells and softer texture than other composite breads and even wheat bread.The above results demonstrated the great potential of EEBRF in improving bread quality,which could be applicated in the development of functional brown rice bread.
