Nonlinear PAPR reducers, such as clipping and companding techniques, are some simple methods used to reduce the Peak-to-Average Power Ratio (PAPR). In this paper, assuming that the baseband OFDM signal is characterize...Nonlinear PAPR reducers, such as clipping and companding techniques, are some simple methods used to reduce the Peak-to-Average Power Ratio (PAPR). In this paper, assuming that the baseband OFDM signal is characterized as a band-limited complex Gaussian process, we investigate the PAPR distribution of an OFDM signal when it is passed through a nonlinear PAPR reducer. The obtained PAPR distribution depends on the nonlinear function which characterizes the PAPR reducer. Later in this paper, we apply the obtained PAPR distribution in the clipping case. The comparisons made between the proposed distribution and that obtained thanks to computer simulations show good agreement.展开更多
A geometrical parameters optimization and reducers selection method was proposed for robotic manipulators design. The Lagrangian approach was employed in deriving the dynamic model of a two-DOF manipulator. The flexib...A geometrical parameters optimization and reducers selection method was proposed for robotic manipulators design. The Lagrangian approach was employed in deriving the dynamic model of a two-DOF manipulator. The flexibility of links and joints was taken into account in the mechanical structure dimensions optimization and reducers selection, in which Timoshenko model was used to discretize the hollow links. Two criteria, i.e. maximization of fundamental frequency and minimization of self-mass/load ratio, were utilized to optimize the manipulators. The NSGA-II (fast elitist nondominated sorting genetic algorithms) was employed to solve the multi-objective optimization problem. How the joints flexibility affects the manipulators design was analyzed and shown in the numerical analysis example. The results indicate that simultaneous consideration of the joints and the links flexibility is very necessary for manipulators optimal design. Finally, several optimal combinations were provided. The effectiveness of the optimization method was proved by comparing with ADAMS simulation results. The self-mass/load ratio error of the two methods is within 10%. The maximum error of the natural frequency by the two methods is 23.74%. The method proposed in this work provides a fast and effective pathway for manipulator design and reducers selection.展开更多
Owing to some excellent properties(such as large transmission ratio, high reliability, high precision,large stiffness, small volume and long service life), filtering reducers are suitable for occasions with high requi...Owing to some excellent properties(such as large transmission ratio, high reliability, high precision,large stiffness, small volume and long service life), filtering reducers are suitable for occasions with high requirements(e.g. space equipment). However, the tough working condition in space(including frequent changes of temperature, and large temperature difference combined with shock loads) may significantly affect the reliability.In this paper, the expressions of the minimal and maximal instantaneous transmission ratios(ITRs) considering temperature correction are developed. The minimal and maximal ITRs from simulation are used to verify the accuracy. Moreover, the reliability of a filtering reducer under different temperatures and the shock load are calculated, respectively. The research is beneficial to the design of spacecraft mechanism.展开更多
Poor solubility often results in low efficacy of antitumor drugs.Nevertheless,limited research has been conducted on the potential decrease in drug efficacy following the self-assembly of hydrophobic pure drugs into n...Poor solubility often results in low efficacy of antitumor drugs.Nevertheless,limited research has been conducted on the potential decrease in drug efficacy following the self-assembly of hydrophobic pure drugs into nanodrugs,and solutions to this problem are even rarer.Loading water-insoluble antitumor drugs into nanocarriers offers a promising solution.However,intricate carrier preparation,limited drug loading capacity,and carrier-associated safety remain key challenges.In this study,based on the discovery that hydrophobic gambogic acid(GA) self-assembles into nanostructures with diminished antitumor efficacy in aqueous environments,we developed a carrier-free nanodrug system,designated as GA-S-S-AS nanoparticles(NPs),characterized by straightforward preparation,high drug loading,fluorescence imaging,tumor-targeting,and responsive drug release in reducing environments.Specifically,the hydrophobic GA was covalently linked to the hydrophilic aptamer through a disulfide bond and then self-assembled into the nanodrugs.About 92 % of drug was encapsulated in self-assembled NPs,demonstrating remarkable stability under physiological conditions and controlled release of GA in the high-glutathione environment characteristic of tumor sites.Furthermore,by utilizing the synergistic interaction between the enhanced permeability and retention(EPR) effect and ligand-receptor active targeting mechanisms,the nanodrugs significantly increased the accumulation of GA at tumor locations.Consequently,the nanodrugs exhibited optimal therapeutic efficacy against the tumor both in vitro and in vivo,significantly inhibiting tumor growth.Furthermore,the nanodrugs demonstrated enhanced biosafety compared to free GA,effectively reducing GA-induced hepatotoxicity.Taken together,these findings underscore the significant potential of this multifunctional carrier-free nanodrugs for the targeted delivery of GA,thereby laying a foundation for future endeavors aimed at developing novel formulations of hydrophobic antitumor drugs.展开更多
Ferroptosis has exhibited great potential in therapies and intracellular reducing agents of sulfur species(RSSs) in the thiol-dependent redox systems are crucial in ferroptosis.This makes the simultaneous detection of...Ferroptosis has exhibited great potential in therapies and intracellular reducing agents of sulfur species(RSSs) in the thiol-dependent redox systems are crucial in ferroptosis.This makes the simultaneous detection of multiple RSSs significant for evaluating ferroptosis therapy.However,the traditional techniques,including fluorescent(FL) imaging and electrospray ionization-based mass spectrometry(MS) detection,cannot achieve the discrimination of different RSSs.Herein,simultaneous MS detection of multiple RSSs,including cysteine(Cys),homocysteine(Hcy),glutathione(GSH) and hydrogen sulfide(H_(2)S),was obtained upon enhancing ionization efficiency by a fluorescent probe(NBD-O-1).Based on the interaction between NBD-O-1 and RSSs,the complex of RSSs with a fragment of NBD-O-1 can be generated,which can be easily ionized for MS detection in the negative mode.Therefore,the intracellular RSSs can be well detected upon the incubation of He La cells with the probe of NBD-O-1,exhibiting the total RSS levels by the FL imaging and further providing expression of each RSS by enhanced MS detection.Furthermore,the RSSs during ferroptosis in He La cells have been evaluated using the present strategy,demonstrating the potential for ferroptosis examinations.This work has made an unconventional application of a fluorescent probe to enhance the detection of multiple RSSs by MS,providing significant molecular information for addressing the ferroptosis mechanism.展开更多
Hainan Province in south China is the country’s second-largest island and the largest free trade port by area.It has entered a historic phase in China’s drive to promote high-level institutional opening up.On 18 Dec...Hainan Province in south China is the country’s second-largest island and the largest free trade port by area.It has entered a historic phase in China’s drive to promote high-level institutional opening up.On 18 December 2025,Hainan officially launched island-wide special customs operations,commonly referred to as“customs closure.”From that date,goods entering or leaving the island,except those traded with the Chinese mainland,are subject to simplified customs procedures and potentially reduced or zero tariffs.展开更多
Traumatic spinal cord injury(SCI)is a pathological condition that impairs both sensorimotor and cognitive functions.While research has long focused on understanding the pathophysiology of SCI and developing treatments...Traumatic spinal cord injury(SCI)is a pathological condition that impairs both sensorimotor and cognitive functions.While research has long focused on understanding the pathophysiology of SCI and developing treatments,only a few studies have investigated the cellular and molecular consequences that occur in the brain after trauma.From the earliest stages,the injury triggers microglial activation,increased neuronal death,and reduced hippocampal neurogenesis in the dentate gyrus.展开更多
Hainan’s special customs operations are expected to significantly reduce the institutional costs of foreign goods flowing into Hainan,enabling the island to function as a gateway for global goods.DAVID Adrian Janke,a...Hainan’s special customs operations are expected to significantly reduce the institutional costs of foreign goods flowing into Hainan,enabling the island to function as a gateway for global goods.DAVID Adrian Janke,a British citizen operating a translation company in Haikou,capital of Hainan Province in south China,witnessed surging orders as special customs operations got underway in the island province on December 18.展开更多
In the article titled“Inhibiting SHP2 reduces glycolysis,promotes microglial M1 polarization,and alleviates secondary inflammation following spinal cord injury in a mouse model,”published in Neural Regeneration Rese...In the article titled“Inhibiting SHP2 reduces glycolysis,promotes microglial M1 polarization,and alleviates secondary inflammation following spinal cord injury in a mouse model,”published in Neural Regeneration Research(Ding et al.,2025),the title was incorrectly presented due to an error during the language polishing process.展开更多
Type 2 diabetes mellitus has central complications:Diabetes,a metabolic disorder primarily characterized by hyperglycemia due to insufficient insulin secretion,or impaired insulin signaling,has significant central com...Type 2 diabetes mellitus has central complications:Diabetes,a metabolic disorder primarily characterized by hyperglycemia due to insufficient insulin secretion,or impaired insulin signaling,has significant central complications.Type 2 diabetes mellitus(T2DM),the most prevalent type of diabetes,affects more than 38 million individuals in the United States(approximately 1 in 10)and is defined by chronic hyperglycemia and insulin resistance,which refers to a reduced cellular response to insulin.展开更多
The growing demand for personalized health care,smart wearables,and advanced environmental monitoring has spurred the development of multifunctional materials that combine flexibility,environmental adaptability,and di...The growing demand for personalized health care,smart wearables,and advanced environmental monitoring has spurred the development of multifunctional materials that combine flexibility,environmental adaptability,and diverse functionalities.However,conventional materials often failed to integrate these attributes simultaneously,hindering their applicability in next-generation technologies.Here,we present an organic-inorganic hybrid crystalline material with a unique sandwich-like architecture,in which a flexible organic crystal core is encased by reduced graphene oxide(rGO)and thermoplastic polyurethane(TPU).This strategic integration endows the material with fluorescence,cryogenic flexibility,and electrical conductivity,while also enabling dual sensing and actuation capabilities.The rGO layer facilitates real-time humidity(25-90%RH)and temperature(25-180℃)sensing through environmental interactions,whereas the differential thermal expansion between TPU and the flexible crystal core drives efficient photothermal actuation at-150℃ for advanced thermal regulation.The hybrid material exhibits stable performance under extreme conditions,making it a promising candidate for biomedical monitoring,flexible electronics,and energy applications.This work establishes hybrid crystalline materials as versatile and scalable platforms for addressing complex technological demands,paving the way for their application in next-generation multifunctional devices.展开更多
BACKGROUND Ulcerative colitis(UC)is a chronic and debilitating inflammatory bowel disease.Cumulative evidence indicates that excess hydrogen peroxide,a potent neutrophilic chemotactic agent,produced by colonic epithel...BACKGROUND Ulcerative colitis(UC)is a chronic and debilitating inflammatory bowel disease.Cumulative evidence indicates that excess hydrogen peroxide,a potent neutrophilic chemotactic agent,produced by colonic epithelial cells has a causal role leading to infiltration of neutrophils into the colonic mucosa and subsequent development of UC.This evidence-based mechanism identifies hydrogen peroxide as a therapeutic target for reducing agents in the treatment of UC.CASE SUMMARY Presented is a 41-year-old female with a 26-year history of refractory UC.Having developed steroid dependence and never achieving complete remission on treatment by conventional and advanced therapies,she began treatment with oral R-dihydrolipoic acid(RDLA),a lipid-soluble reducing agent with intracellular site of action.Within a week,rectal bleeding ceased.She was asymptomatic for three years until a highly stressful experience,when she noticed blood in her stool.RDLA was discontinued,and she began treatment with oral sodium thiosulfate pentahydrate(STS),a reducing agent with extracellular site of action.After a week,rectal bleeding ceased,and she resumed oral RDLA and discontinued STS.To date,she remains asymptomatic with normal stool calprotectin while on RDLA.CONCLUSION STS and RDLA are reducing agents that serve as highly effective and safe therapy for the induction and maintenance of remission in UC,even in patients refractory or poorly controlled by conventional and advanced therapies.Should preliminary findings be validated by subsequent clinical trials,the use of reducing agents could potentially prevent thousands of colectomies and represent a paradigm shift in the treatment of UC.展开更多
In vitro maturation(IvM)of human oocytes offers cost efficiency and minimal invasiveness,serving as a valuable supplementary tool in assisted reproduction for fertility preservation,ovarian hyperstimulation syndrome p...In vitro maturation(IvM)of human oocytes offers cost efficiency and minimal invasiveness,serving as a valuable supplementary tool in assisted reproduction for fertility preservation,ovarian hyperstimulation syndrome prevention,and other reproductive strategies.Despite its availability for three decades,the clinical use of IVM remains limited due to efficacy and safety concerns.This study examines the DNA methylation profile of IVM oocytes collected during laparoscopic/hysteroscopic surgeries compared to in vivo matured oocytes via reduced representation bisulfite sequencing.Results indicate IVM oocytes exhibit a higher global methylation level.Differentially methylated regions(DMRs)analysis reveals that the in vitro group displays more hypermethylated and fewer hypomethylated DMRs compared to the in vivo group.Additionally,the in vitro group exhibits a higher level of non-CpG methylation than the in vivo group.However,no significant correlation between methylation levels and transcriptional activity in these oocytes is found,especially for those specific imprinted genes or genes related to embryonic development.These findings shed light on the epigenetic landscape of IvM oocytes,contributing to the ongoing assessment of their clinical feasibility and safety in assisted reproduction.展开更多
Generalised reduced masses with a set of equations governing the three relative motions between two of 3-bodies in their gravitational field are established,of which the dynamic characteristics of 3-body dynamics,fund...Generalised reduced masses with a set of equations governing the three relative motions between two of 3-bodies in their gravitational field are established,of which the dynamic characteristics of 3-body dynamics,fundamental bases of this paper,are revealed.Based on these findings,an equivalent system is developed,which is a 2-body system with its total mass,constant angular momentum,kinetic and potential energies same as the total ones of three relative motions,so that it can be solved using the well-known theory of the 2-body system.From the solution of an equivalent system with the revealed characteristics of three relative motions,the general theoretical solutions of the 3-body system are obtained in the curve-integration forms along the orbits in the imaged radial motion space.The possible periodical orbits with generalised Kepler’s law are presented.Following the description and mathematical demonstrations of the proposed methods,the examples including Euler’s/Lagrange’s problems,and a reported numerical one are solved to validate the proposed methods.The methods derived from the 3-body system are extended to N-body problems.展开更多
Silicon-air batteries(SABs),a new type of semiconductor air battery,have a high energy density.However,some side reactions in SABs cause Si anodes to be covered by a passivation layer to prevent continuous discharge,a...Silicon-air batteries(SABs),a new type of semiconductor air battery,have a high energy density.However,some side reactions in SABs cause Si anodes to be covered by a passivation layer to prevent continuous discharge,and the anode utilization rate is low.In this work,reduced graphene oxide(RGO)fabricated via high-temperature annealing or L-ascorbic acid(L.AA)reduction was first used to obtain Si nanowires/RGO-1000(Si NWs/RGO-1000)and Si nanowires/RGO-L.AA(Si NWs/RGO-L.AA)composite anodes for SABs.It was found that RGO suppressed the passivation and self-corrosion reactions and that SABs using Si NWs/RGO-L.AA as the anode can discharge for more than 700 h,breaking the previous performance of SABs,and that the specific capacity was increased by 90.8%compared to bare Si.This work provides a new solution for the design of high specific capacity SABs with nanostructures and anode protective layers.展开更多
Fast electron-hole recombination issues during titanium dioxide(TiO_(2))photocatalysis limit its application in preventing bacterial infection during bone defect repair.In this study,TiO_(2)@reduced graphene oxide(rGO...Fast electron-hole recombination issues during titanium dioxide(TiO_(2))photocatalysis limit its application in preventing bacterial infection during bone defect repair.In this study,TiO_(2)@reduced graphene oxide(rGO)composites were synthesized using a hydrothermal method in which rGO,which possesses very high electrical conductivity,promotes the separation of photoelectron-hole pairs of TiO_(2),thus improving the efficiency of photocatalytic production of reactive oxygen species(ROS).Subsequently,TiO_(2)@rGO composites were introduced into poly-L-lactic acid(PLLA)to prepare bone scaffolds with photocatalytic antibacterial function via selective laser sintering.The results showed that TiO_(2)grew on the surface of rGO and formed a covalent bond connection(Ti-O-C)with rGO.A decreased electrochemical impedance of TiO_(2)@rGO composites was observed,and the transient photocurrent intensity increased from 0.05 to 0.5μA/cm^(2).Analysis of electron spin resonance found that the photocatalytic products of TiO_(2)were·OH and·O^(2-),two kinds of ROS capable of killing bacteria via disrupting the structure of the bacterial membrane in vitro.Antibacterial experiments showed that the PLLA/TiO_(2)@rGO scaffolds had good antibacterial properties against Escherichia coli and Staphylococcus aureus.Finally,we report that these scaffolds exhibited both enhanced mechanical properties due to the addition of TiO_(2)@rGO as a reinforcement material and good biocompatibility during cell proliferation.展开更多
The prevalence of Class Ⅲ malocclusion varies among different countries and regions. The populations from Southeast Asian countries (Chinese and Malaysian) showed the highest prevalence rate of 15.8%, which can serio...The prevalence of Class Ⅲ malocclusion varies among different countries and regions. The populations from Southeast Asian countries (Chinese and Malaysian) showed the highest prevalence rate of 15.8%, which can seriously affect oral function, facial appearance, and mental health. As anterior crossbite tends to worsen with growth, early orthodontic treatment can harness growth potential to normalize maxillofacial development or reduce skeletal malformation severity, thereby reducing the difficulty and shortening the treatment cycle of later-stage treatment. This is beneficial for the physical and mental growth of children. Therefore,early orthodontic treatment for Class Ⅲ malocclusion is particularly important. Determining the optimal timing for early orthodontic treatment requires a comprehensive assessment of clinical manifestations, dental age, and skeletal age, and can lead to better results with less effort. Currently, standardized treatment guidelines for early orthodontic treatment of Class Ⅲ malocclusion are lacking. This review provides a comprehensive summary of the etiology, clinical manifestations, classification, and early orthodontic techniques for Class Ⅲ malocclusion, along with systematic discussions on selecting early treatment plans. The purpose of this expert consensus is to standardize clinical practices and improve the treatment outcomes of Class Ⅲ malocclusion through early orthodontic treatment.展开更多
Macroalgae dominate nutrient dynamics and function as high-value foods for microbial,meio-and macrofaunal communities in coastal ecosystems.Because of this vital role,it is important to clarify the physiological infor...Macroalgae dominate nutrient dynamics and function as high-value foods for microbial,meio-and macrofaunal communities in coastal ecosystems.Because of this vital role,it is important to clarify the physiological information associated with environmental changes as it reflects their growth potential.To evaluate the effects of the changes in salinity and nutrients,the photosynthetic efficiency of a green macroalga Ulva fasciata from the Daya Bay was tested at a range of salinity(i.e.,31 to 10 psu)and nitrogen content(i.e.,5 to 60μmol L^(-1)).The results showed that cellular chlorophyll a(Chl a),carbohydrate and protein contents of U.fasciata were increased due to reduced salinity,and were decreased by interactive nitrogen enrichment.Within a short culture period(i.e.,18 h),the reduced salinity decreased the maximum photosynthetic efficiency(rETRmax and Pmax)derived from the rapid light response curve and photosynthetic oxygen evolution rate versus irradiance curve,respectively,as well as the saturation irradiance(E_(K)).This reducing effect diminished with enlonged cultivation time and reversed to a stimulating effect after 24 h of cultivation.The nitrogen enrichment stimulated the rETRmax and Pmax,as well as the E_(K),regardless of salinity,especially within short-term cultivation period(i.e.,<24 h).In addition,our results indicate that seawater freshening lowers the photosynthetic efficiency of U.fasciata in the short term,which is mitigated by nitrogen enrichment,but stimulates it in the long term,providing insight into how macroalgae thrive in coastal or estuarine waters where salinity and nutrients normally covary strongly.展开更多
To make agricultural systems sustainable in terms of their greenness and efficiency,optimizing the tillage and fertilization practices is essential.To assess the effects of tilling and fertilization practices in wheat...To make agricultural systems sustainable in terms of their greenness and efficiency,optimizing the tillage and fertilization practices is essential.To assess the effects of tilling and fertilization practices in wheat-maize cropping systems,a three-year field experiment was designed to quantify the carbon footprint(CF)and energy efficiency of the cropping systems in the North China Plain.The study parameters included four tillage practices(no tillage(NT),conventional tillage(CT),rotary tillage(RT),and subsoiling rotary tillage(SRT))and two fertilizer regimes(inorganic fertilizer(IF)and hybrid fertilizer with organic and inorganic components(HF)).The results indicated that the most prominent energy inputs and greenhouse gas(GHG)emissions could be ascribed to the use of fertilizers and fuel consumption.Under the same fertilization regime,ranking the tillage patterns with respect to the value of the crop yield,profit,CF,energy use efficiency(EUE)or energy productivity(EP)for either wheat or maize always gave the same sequence of SRT>RT>CT>NT.For the same tillage,the energy consumption associated with HF was higher than IF,but its GHG emissions and CF were lower while the yield and profit were higher.In terms of overall performance,tilling is more beneficial than NT,and reduced tillage practices(RT and SRT)are more beneficial than CT.The fertilization regime with the best overall performance was HF.Combining SRT with HF has significant potential for reducing CF and increasing EUE,thereby improving sustainability.Adopting measures that promote these optimizations can help to overcome the challenges posed by a lack of food security,energy crises and ecological stress.展开更多
文摘Nonlinear PAPR reducers, such as clipping and companding techniques, are some simple methods used to reduce the Peak-to-Average Power Ratio (PAPR). In this paper, assuming that the baseband OFDM signal is characterized as a band-limited complex Gaussian process, we investigate the PAPR distribution of an OFDM signal when it is passed through a nonlinear PAPR reducer. The obtained PAPR distribution depends on the nonlinear function which characterizes the PAPR reducer. Later in this paper, we apply the obtained PAPR distribution in the clipping case. The comparisons made between the proposed distribution and that obtained thanks to computer simulations show good agreement.
基金Project(2009AA04Z216) supported by the National High-Tech Research and Development Program (863 Program) of ChinaProject(2009ZX04013-011) supported by the National Science and Technology Major Project of ChinaProject supported by the HIT Oversea Talents Introduction Program,China
文摘A geometrical parameters optimization and reducers selection method was proposed for robotic manipulators design. The Lagrangian approach was employed in deriving the dynamic model of a two-DOF manipulator. The flexibility of links and joints was taken into account in the mechanical structure dimensions optimization and reducers selection, in which Timoshenko model was used to discretize the hollow links. Two criteria, i.e. maximization of fundamental frequency and minimization of self-mass/load ratio, were utilized to optimize the manipulators. The NSGA-II (fast elitist nondominated sorting genetic algorithms) was employed to solve the multi-objective optimization problem. How the joints flexibility affects the manipulators design was analyzed and shown in the numerical analysis example. The results indicate that simultaneous consideration of the joints and the links flexibility is very necessary for manipulators optimal design. Finally, several optimal combinations were provided. The effectiveness of the optimization method was proved by comparing with ADAMS simulation results. The self-mass/load ratio error of the two methods is within 10%. The maximum error of the natural frequency by the two methods is 23.74%. The method proposed in this work provides a fast and effective pathway for manipulator design and reducers selection.
基金the Open Foundation of the State Key Laboratory of Mechanical Transmissions of Chongqing University(No.SKLMT-KFKT-201601)
文摘Owing to some excellent properties(such as large transmission ratio, high reliability, high precision,large stiffness, small volume and long service life), filtering reducers are suitable for occasions with high requirements(e.g. space equipment). However, the tough working condition in space(including frequent changes of temperature, and large temperature difference combined with shock loads) may significantly affect the reliability.In this paper, the expressions of the minimal and maximal instantaneous transmission ratios(ITRs) considering temperature correction are developed. The minimal and maximal ITRs from simulation are used to verify the accuracy. Moreover, the reliability of a filtering reducer under different temperatures and the shock load are calculated, respectively. The research is beneficial to the design of spacecraft mechanism.
基金the National Natural Science Foundation of China (Nos.21907076 and 31901908)the Natural Science Foundation of Tianjin (No.22JCQNJC01570)。
文摘Poor solubility often results in low efficacy of antitumor drugs.Nevertheless,limited research has been conducted on the potential decrease in drug efficacy following the self-assembly of hydrophobic pure drugs into nanodrugs,and solutions to this problem are even rarer.Loading water-insoluble antitumor drugs into nanocarriers offers a promising solution.However,intricate carrier preparation,limited drug loading capacity,and carrier-associated safety remain key challenges.In this study,based on the discovery that hydrophobic gambogic acid(GA) self-assembles into nanostructures with diminished antitumor efficacy in aqueous environments,we developed a carrier-free nanodrug system,designated as GA-S-S-AS nanoparticles(NPs),characterized by straightforward preparation,high drug loading,fluorescence imaging,tumor-targeting,and responsive drug release in reducing environments.Specifically,the hydrophobic GA was covalently linked to the hydrophilic aptamer through a disulfide bond and then self-assembled into the nanodrugs.About 92 % of drug was encapsulated in self-assembled NPs,demonstrating remarkable stability under physiological conditions and controlled release of GA in the high-glutathione environment characteristic of tumor sites.Furthermore,by utilizing the synergistic interaction between the enhanced permeability and retention(EPR) effect and ligand-receptor active targeting mechanisms,the nanodrugs significantly increased the accumulation of GA at tumor locations.Consequently,the nanodrugs exhibited optimal therapeutic efficacy against the tumor both in vitro and in vivo,significantly inhibiting tumor growth.Furthermore,the nanodrugs demonstrated enhanced biosafety compared to free GA,effectively reducing GA-induced hepatotoxicity.Taken together,these findings underscore the significant potential of this multifunctional carrier-free nanodrugs for the targeted delivery of GA,thereby laying a foundation for future endeavors aimed at developing novel formulations of hydrophobic antitumor drugs.
基金supported by the National Key Research and Development Program of China (No.2024YFA1509600)National Natural Science Foundation of China (Nos.22474010 and 22274012)the Fundamental Research Funds for the Central Universities (No.2233300007)。
文摘Ferroptosis has exhibited great potential in therapies and intracellular reducing agents of sulfur species(RSSs) in the thiol-dependent redox systems are crucial in ferroptosis.This makes the simultaneous detection of multiple RSSs significant for evaluating ferroptosis therapy.However,the traditional techniques,including fluorescent(FL) imaging and electrospray ionization-based mass spectrometry(MS) detection,cannot achieve the discrimination of different RSSs.Herein,simultaneous MS detection of multiple RSSs,including cysteine(Cys),homocysteine(Hcy),glutathione(GSH) and hydrogen sulfide(H_(2)S),was obtained upon enhancing ionization efficiency by a fluorescent probe(NBD-O-1).Based on the interaction between NBD-O-1 and RSSs,the complex of RSSs with a fragment of NBD-O-1 can be generated,which can be easily ionized for MS detection in the negative mode.Therefore,the intracellular RSSs can be well detected upon the incubation of He La cells with the probe of NBD-O-1,exhibiting the total RSS levels by the FL imaging and further providing expression of each RSS by enhanced MS detection.Furthermore,the RSSs during ferroptosis in He La cells have been evaluated using the present strategy,demonstrating the potential for ferroptosis examinations.This work has made an unconventional application of a fluorescent probe to enhance the detection of multiple RSSs by MS,providing significant molecular information for addressing the ferroptosis mechanism.
文摘Hainan Province in south China is the country’s second-largest island and the largest free trade port by area.It has entered a historic phase in China’s drive to promote high-level institutional opening up.On 18 December 2025,Hainan officially launched island-wide special customs operations,commonly referred to as“customs closure.”From that date,goods entering or leaving the island,except those traded with the Chinese mainland,are subject to simplified customs procedures and potentially reduced or zero tariffs.
文摘Traumatic spinal cord injury(SCI)is a pathological condition that impairs both sensorimotor and cognitive functions.While research has long focused on understanding the pathophysiology of SCI and developing treatments,only a few studies have investigated the cellular and molecular consequences that occur in the brain after trauma.From the earliest stages,the injury triggers microglial activation,increased neuronal death,and reduced hippocampal neurogenesis in the dentate gyrus.
文摘Hainan’s special customs operations are expected to significantly reduce the institutional costs of foreign goods flowing into Hainan,enabling the island to function as a gateway for global goods.DAVID Adrian Janke,a British citizen operating a translation company in Haikou,capital of Hainan Province in south China,witnessed surging orders as special customs operations got underway in the island province on December 18.
文摘In the article titled“Inhibiting SHP2 reduces glycolysis,promotes microglial M1 polarization,and alleviates secondary inflammation following spinal cord injury in a mouse model,”published in Neural Regeneration Research(Ding et al.,2025),the title was incorrectly presented due to an error during the language polishing process.
基金supported by grants from NIH T32(DK007260,to WC)the Steno North American Fellowship awarded by the Novo Nordisk Foundation(NNF23OC0087108,to WC)+6 种基金STI2030-Major Projects(2021ZD0202700,to HY)the National Natural Science Foundation of China(32241004,to HY)the Natural Science Foundation of Zhejiang Province of China(LR24C090001,to HY)Key R&D Program of Zhejiang Province(2024SSYS0017,to HY)CAMS Innovation Fund for Medical Sciences(2019-12M-5-057,to HY)Fundamental Research Funds for the Central Universities(226-2022-00193,to HY)the Non-profit Central Research Institute Fund of Chinese Academy of Medical Sciences(2023-PT310-01,to HY)。
文摘Type 2 diabetes mellitus has central complications:Diabetes,a metabolic disorder primarily characterized by hyperglycemia due to insufficient insulin secretion,or impaired insulin signaling,has significant central complications.Type 2 diabetes mellitus(T2DM),the most prevalent type of diabetes,affects more than 38 million individuals in the United States(approximately 1 in 10)and is defined by chronic hyperglycemia and insulin resistance,which refers to a reduced cellular response to insulin.
基金support from the National Natural Science Foundation of China(52373181 and 52173164)the Natural Science Foundation of Jilin Province(20250102120JC and 20230101038JC)+1 种基金the Postdoctoral Fellowship Program of China Postdoctoral Science Foundation(GZB20240259)the Project funded by China Postdoctoral Science Foundation(2024M761121 and 2025T180139).
文摘The growing demand for personalized health care,smart wearables,and advanced environmental monitoring has spurred the development of multifunctional materials that combine flexibility,environmental adaptability,and diverse functionalities.However,conventional materials often failed to integrate these attributes simultaneously,hindering their applicability in next-generation technologies.Here,we present an organic-inorganic hybrid crystalline material with a unique sandwich-like architecture,in which a flexible organic crystal core is encased by reduced graphene oxide(rGO)and thermoplastic polyurethane(TPU).This strategic integration endows the material with fluorescence,cryogenic flexibility,and electrical conductivity,while also enabling dual sensing and actuation capabilities.The rGO layer facilitates real-time humidity(25-90%RH)and temperature(25-180℃)sensing through environmental interactions,whereas the differential thermal expansion between TPU and the flexible crystal core drives efficient photothermal actuation at-150℃ for advanced thermal regulation.The hybrid material exhibits stable performance under extreme conditions,making it a promising candidate for biomedical monitoring,flexible electronics,and energy applications.This work establishes hybrid crystalline materials as versatile and scalable platforms for addressing complex technological demands,paving the way for their application in next-generation multifunctional devices.
文摘BACKGROUND Ulcerative colitis(UC)is a chronic and debilitating inflammatory bowel disease.Cumulative evidence indicates that excess hydrogen peroxide,a potent neutrophilic chemotactic agent,produced by colonic epithelial cells has a causal role leading to infiltration of neutrophils into the colonic mucosa and subsequent development of UC.This evidence-based mechanism identifies hydrogen peroxide as a therapeutic target for reducing agents in the treatment of UC.CASE SUMMARY Presented is a 41-year-old female with a 26-year history of refractory UC.Having developed steroid dependence and never achieving complete remission on treatment by conventional and advanced therapies,she began treatment with oral R-dihydrolipoic acid(RDLA),a lipid-soluble reducing agent with intracellular site of action.Within a week,rectal bleeding ceased.She was asymptomatic for three years until a highly stressful experience,when she noticed blood in her stool.RDLA was discontinued,and she began treatment with oral sodium thiosulfate pentahydrate(STS),a reducing agent with extracellular site of action.After a week,rectal bleeding ceased,and she resumed oral RDLA and discontinued STS.To date,she remains asymptomatic with normal stool calprotectin while on RDLA.CONCLUSION STS and RDLA are reducing agents that serve as highly effective and safe therapy for the induction and maintenance of remission in UC,even in patients refractory or poorly controlled by conventional and advanced therapies.Should preliminary findings be validated by subsequent clinical trials,the use of reducing agents could potentially prevent thousands of colectomies and represent a paradigm shift in the treatment of UC.
基金supported by funding from the National Natural Science Foundation of China(81971349 and 81300456).
文摘In vitro maturation(IvM)of human oocytes offers cost efficiency and minimal invasiveness,serving as a valuable supplementary tool in assisted reproduction for fertility preservation,ovarian hyperstimulation syndrome prevention,and other reproductive strategies.Despite its availability for three decades,the clinical use of IVM remains limited due to efficacy and safety concerns.This study examines the DNA methylation profile of IVM oocytes collected during laparoscopic/hysteroscopic surgeries compared to in vivo matured oocytes via reduced representation bisulfite sequencing.Results indicate IVM oocytes exhibit a higher global methylation level.Differentially methylated regions(DMRs)analysis reveals that the in vitro group displays more hypermethylated and fewer hypomethylated DMRs compared to the in vivo group.Additionally,the in vitro group exhibits a higher level of non-CpG methylation than the in vivo group.However,no significant correlation between methylation levels and transcriptional activity in these oocytes is found,especially for those specific imprinted genes or genes related to embryonic development.These findings shed light on the epigenetic landscape of IvM oocytes,contributing to the ongoing assessment of their clinical feasibility and safety in assisted reproduction.
文摘Generalised reduced masses with a set of equations governing the three relative motions between two of 3-bodies in their gravitational field are established,of which the dynamic characteristics of 3-body dynamics,fundamental bases of this paper,are revealed.Based on these findings,an equivalent system is developed,which is a 2-body system with its total mass,constant angular momentum,kinetic and potential energies same as the total ones of three relative motions,so that it can be solved using the well-known theory of the 2-body system.From the solution of an equivalent system with the revealed characteristics of three relative motions,the general theoretical solutions of the 3-body system are obtained in the curve-integration forms along the orbits in the imaged radial motion space.The possible periodical orbits with generalised Kepler’s law are presented.Following the description and mathematical demonstrations of the proposed methods,the examples including Euler’s/Lagrange’s problems,and a reported numerical one are solved to validate the proposed methods.The methods derived from the 3-body system are extended to N-body problems.
基金supported by the National Natural Science Foundation of China(No.61904073)Spring City Plan-Special Program for Young Talents(No.K202005007)+4 种基金Yunnan Talents Support Plan for Yong Talents(No.XDYC-QNRC-20220482)Yunnan Local Colleges Applied Basic Research Projects(No.202101BA070001-138)Scientific Research Fund of Yunnan Education Department(No.2023Y0883)Frontier Research Team of Kunming University 2023Key Laboratory of Artificial Microstructures in Yunnan Higher Education。
文摘Silicon-air batteries(SABs),a new type of semiconductor air battery,have a high energy density.However,some side reactions in SABs cause Si anodes to be covered by a passivation layer to prevent continuous discharge,and the anode utilization rate is low.In this work,reduced graphene oxide(RGO)fabricated via high-temperature annealing or L-ascorbic acid(L.AA)reduction was first used to obtain Si nanowires/RGO-1000(Si NWs/RGO-1000)and Si nanowires/RGO-L.AA(Si NWs/RGO-L.AA)composite anodes for SABs.It was found that RGO suppressed the passivation and self-corrosion reactions and that SABs using Si NWs/RGO-L.AA as the anode can discharge for more than 700 h,breaking the previous performance of SABs,and that the specific capacity was increased by 90.8%compared to bare Si.This work provides a new solution for the design of high specific capacity SABs with nanostructures and anode protective layers.
基金supported by the following funds:The National Natural Science Foundation of China(Nos.52275393,51935014,and 82072084)Jiangxi Provincial Natural Science Foundation of China(No.20224ACB204013)+2 种基金The Project of State Key Laboratory of Precision Manufacturing for Extreme Service Performancethe National Key Research and Development Program of China(No.2023YFB4605800)the Independent Exploration and Innovation Project of Central South University(No.1053320221707).
文摘Fast electron-hole recombination issues during titanium dioxide(TiO_(2))photocatalysis limit its application in preventing bacterial infection during bone defect repair.In this study,TiO_(2)@reduced graphene oxide(rGO)composites were synthesized using a hydrothermal method in which rGO,which possesses very high electrical conductivity,promotes the separation of photoelectron-hole pairs of TiO_(2),thus improving the efficiency of photocatalytic production of reactive oxygen species(ROS).Subsequently,TiO_(2)@rGO composites were introduced into poly-L-lactic acid(PLLA)to prepare bone scaffolds with photocatalytic antibacterial function via selective laser sintering.The results showed that TiO_(2)grew on the surface of rGO and formed a covalent bond connection(Ti-O-C)with rGO.A decreased electrochemical impedance of TiO_(2)@rGO composites was observed,and the transient photocurrent intensity increased from 0.05 to 0.5μA/cm^(2).Analysis of electron spin resonance found that the photocatalytic products of TiO_(2)were·OH and·O^(2-),two kinds of ROS capable of killing bacteria via disrupting the structure of the bacterial membrane in vitro.Antibacterial experiments showed that the PLLA/TiO_(2)@rGO scaffolds had good antibacterial properties against Escherichia coli and Staphylococcus aureus.Finally,we report that these scaffolds exhibited both enhanced mechanical properties due to the addition of TiO_(2)@rGO as a reinforcement material and good biocompatibility during cell proliferation.
文摘The prevalence of Class Ⅲ malocclusion varies among different countries and regions. The populations from Southeast Asian countries (Chinese and Malaysian) showed the highest prevalence rate of 15.8%, which can seriously affect oral function, facial appearance, and mental health. As anterior crossbite tends to worsen with growth, early orthodontic treatment can harness growth potential to normalize maxillofacial development or reduce skeletal malformation severity, thereby reducing the difficulty and shortening the treatment cycle of later-stage treatment. This is beneficial for the physical and mental growth of children. Therefore,early orthodontic treatment for Class Ⅲ malocclusion is particularly important. Determining the optimal timing for early orthodontic treatment requires a comprehensive assessment of clinical manifestations, dental age, and skeletal age, and can lead to better results with less effort. Currently, standardized treatment guidelines for early orthodontic treatment of Class Ⅲ malocclusion are lacking. This review provides a comprehensive summary of the etiology, clinical manifestations, classification, and early orthodontic techniques for Class Ⅲ malocclusion, along with systematic discussions on selecting early treatment plans. The purpose of this expert consensus is to standardize clinical practices and improve the treatment outcomes of Class Ⅲ malocclusion through early orthodontic treatment.
基金funded by the National Key Research and Development Program of China(No.20022YFC3102405)the National Natural Science Foundation of China(Nos.42425004,32371665)the Natural Science Foundation of Guangdong Province(Nos.2022A1515011461,2022A1515011831)。
文摘Macroalgae dominate nutrient dynamics and function as high-value foods for microbial,meio-and macrofaunal communities in coastal ecosystems.Because of this vital role,it is important to clarify the physiological information associated with environmental changes as it reflects their growth potential.To evaluate the effects of the changes in salinity and nutrients,the photosynthetic efficiency of a green macroalga Ulva fasciata from the Daya Bay was tested at a range of salinity(i.e.,31 to 10 psu)and nitrogen content(i.e.,5 to 60μmol L^(-1)).The results showed that cellular chlorophyll a(Chl a),carbohydrate and protein contents of U.fasciata were increased due to reduced salinity,and were decreased by interactive nitrogen enrichment.Within a short culture period(i.e.,18 h),the reduced salinity decreased the maximum photosynthetic efficiency(rETRmax and Pmax)derived from the rapid light response curve and photosynthetic oxygen evolution rate versus irradiance curve,respectively,as well as the saturation irradiance(E_(K)).This reducing effect diminished with enlonged cultivation time and reversed to a stimulating effect after 24 h of cultivation.The nitrogen enrichment stimulated the rETRmax and Pmax,as well as the E_(K),regardless of salinity,especially within short-term cultivation period(i.e.,<24 h).In addition,our results indicate that seawater freshening lowers the photosynthetic efficiency of U.fasciata in the short term,which is mitigated by nitrogen enrichment,but stimulates it in the long term,providing insight into how macroalgae thrive in coastal or estuarine waters where salinity and nutrients normally covary strongly.
基金supported by research grants from the Natural Science Foundation of Shandong Province,China(ZR2020MC092)the Key Research and Development Project of Shandong Province,China(2019TSCYCX-33)the Key Research and Development Project of Shandong Province,China(LJNY202025).
文摘To make agricultural systems sustainable in terms of their greenness and efficiency,optimizing the tillage and fertilization practices is essential.To assess the effects of tilling and fertilization practices in wheat-maize cropping systems,a three-year field experiment was designed to quantify the carbon footprint(CF)and energy efficiency of the cropping systems in the North China Plain.The study parameters included four tillage practices(no tillage(NT),conventional tillage(CT),rotary tillage(RT),and subsoiling rotary tillage(SRT))and two fertilizer regimes(inorganic fertilizer(IF)and hybrid fertilizer with organic and inorganic components(HF)).The results indicated that the most prominent energy inputs and greenhouse gas(GHG)emissions could be ascribed to the use of fertilizers and fuel consumption.Under the same fertilization regime,ranking the tillage patterns with respect to the value of the crop yield,profit,CF,energy use efficiency(EUE)or energy productivity(EP)for either wheat or maize always gave the same sequence of SRT>RT>CT>NT.For the same tillage,the energy consumption associated with HF was higher than IF,but its GHG emissions and CF were lower while the yield and profit were higher.In terms of overall performance,tilling is more beneficial than NT,and reduced tillage practices(RT and SRT)are more beneficial than CT.The fertilization regime with the best overall performance was HF.Combining SRT with HF has significant potential for reducing CF and increasing EUE,thereby improving sustainability.Adopting measures that promote these optimizations can help to overcome the challenges posed by a lack of food security,energy crises and ecological stress.
