Red and blue light illumination has been reported to significantly affect plantlet growth.Potato is an important food and feed crop in the world and potato plantlet cultured in vitro plays an important role in potato ...Red and blue light illumination has been reported to significantly affect plantlet growth.Potato is an important food and feed crop in the world and potato plantlet cultured in vitro plays an important role in potato production.However,few studies have documented the effects of red and blue light on the growth of potato plantlets revealed at the transcriptome level.The objective of this study was to determine the growth and physiological responses of potato plantlets cultured in vitro under monochromatic red(RR),monochromatic blue(BB)as well as combined red and blue(RB)LEDs using the RNA-Seq technique.In total,3150 and 814 differentially expressed genes(DEGs)were detected in potato plantlets under RR and BB,respectively,compared to RB(used as control).Compared to the control,the DEGs enriched in"photosynthesis"and"photosynthesis-antenna proteins"metabolic pathways were up-regulated and down-regulated by BB and RR,respectively,which might be responsible for the increases and decreases of maximum quantum yield(F_(v)/F_(m)),photochemical quantum yield(φ_(PSII)),photochemical quenching(q_(P))and electron transfer rate(ETR)in BB and RR,respectively.Potato plantlets exhibited dwarfed stems and extended leaves under BB,whereas elongated stems and small leaves were induced under RR.These dramatically altered plantlet phenotypes were associated with variable levels of endogenous plant hormones gibberellin(GAs),indoleacetic acid(IAA)and cytokinins(CKs),as assessed in stems and leaves of potato plantlets.In addition,monochromatic red and blue LEDs trigged the opposite expression profiles of DEGs identified in the"plant hormone signal transduction"metabolic pathway,which were closely related to the endogenous plant hormone levels in potato plantlets.Our results provide insights into the responses of potato plantlets cultured in vitro to red and blue LEDs at the transcriptomic level and may contribute to improvements in the micro-propagation of potato plantlets cultured in vitro from the light spectrum aspect.展开更多
Lycopene, one of the strongest natural antioxidants known and the main carotene in ripe tomato, is very important for human health. Light is well known to be one of the most important environmental stimuli influencing...Lycopene, one of the strongest natural antioxidants known and the main carotene in ripe tomato, is very important for human health. Light is well known to be one of the most important environmental stimuli influencing lycopene biosynthesis; specifically, red light induces higher lycopene content in tomato. However, whether blue light promotes lycopene synthesis remains elusive and exactly how light stimulation promotes lycopene synthesis remains unclear. We applied supplemental blue and red lighting on tomato plants at anthesis to monitor the effect of supplemental blue and red lighting on lycopene synthesis. Our results showed that supplemental blue/red lighting induced higher lycopene content in tomato fruits; furthermore, we found that the expression of key genes in the lycopene synthesis pathway was induced by supplemented blue/red light. The expression of light signaling components, such as red-light receptor phytochromes(PHYs), blue-light receptor cryptochromes(CRYs) and light interaction factors, phytochrome-interacting factors(PIFs) and ELONGATED HYPOCOTYL 5(HY5) were up-or down-regulated by blue/red lighting. Thus, blue and red light increased lycopene content in tomatoes by inducing light receptors that modulate HY5 and PIFs activation to mediate phytoene synthase 1(PSY1) gene expression. These results provide a sound theoretical basis for further elucidation of the light regulating mechanism of lycopene synthesis in tomatoes, and for instituting a new generation of technological innovations for the enhancement of lycopene accumulation in crop production.展开更多
Fluorescence enhancement of red and blue concurrently emitting Ba3MgSi2O8:Eu2+,Mn2+ phosphors for plant cultivation has been investigated by Dy3+ addition.The Ba3MgSi2O8:Eu2+,Mn2+,Dy3+(BMS-EMD) phosphors have two-colo...Fluorescence enhancement of red and blue concurrently emitting Ba3MgSi2O8:Eu2+,Mn2+ phosphors for plant cultivation has been investigated by Dy3+ addition.The Ba3MgSi2O8:Eu2+,Mn2+,Dy3+(BMS-EMD) phosphors have two-color emissions at the wavelength peak values of 437 nm and 620 nm at the excitation of 350 nm.The two emission bands are coincident with the absorption spectrum for photosynthesis of plants.An obvious enhancement effect has been observed upon addition of Dy3+ with amount of 0.03 mol%,in which the ...展开更多
Blue and red light are spectral wavelengths more effective for plants. The effects of different ratios of red and blue light （R/B=2, R/B=4, R/B=8, R/B=12） provided by LEDs on morphology and photosynthetic characteri...Blue and red light are spectral wavelengths more effective for plants. The effects of different ratios of red and blue light （R/B=2, R/B=4, R/B=8, R/B=12） provided by LEDs on morphology and photosynthetic characteristics of tomato seedlings were studied. The results showed that plant height, stem diameter, fresh weight, dry weight, seedling index and G value increased with the increase of R/B ratio until 8. On the contrary, SPDA value decreased with the increase of R/B ratio. Photosynthetic characteristics were measured by CO 2 assimilation （ Pn ）, stomatal conductance （ gs ） and intracellular CO 2 concentration （ Ci） . Pn and gs decreased with the increase of R/B ratio. Furthermore, similar trend was investigated in photochemical quenching （qP） and electron transport rate （ETR）. Results of this study suggest that compared with white LED, appropriate combination of red and blue light can enhance plant growth and photosynthetic characteristics, and the optimal blue/red ratio for tomato growth was R/B=8.展开更多
The quality of light is an important abiotic factor that affects the growth and development of photosynthetic organisms.In this study,we exposed the unicellular green alga Dunaliella salina to red(660 nm)and blue(450 ...The quality of light is an important abiotic factor that affects the growth and development of photosynthetic organisms.In this study,we exposed the unicellular green alga Dunaliella salina to red(660 nm)and blue(450 nm)light and analyzed the cell growth,total carotenoid content,and transcriptomes.The growth of D.salina was enhanced by illumination with red light,whereas blue light was not able to promote the algal growth.In contrast,the total carotenoid content increased under both red and blue light.The RNA of D.salina was sequenced and the transcriptomic response of algal cells to red and blue light was investigated.Six transcripts encoding for the blue light receptor cryptochrome were identified,and transcripts involved in the carotenoid metabolism were up-regulated under both red and blue light.Transcripts encoding for photoprotective enzymes related to the scavenging of reactive oxygen species were up-regulated under blue light.The present transcriptomic study provides a more comprehensive understanding of carotenoid biosynthesis in D.salina under different wavelengths of light.展开更多
以C.I.Reactive Red 241、C.I.Disperse Blue 56模拟染料废水为对象,研究了电解法处理该类染料废水的优化条件。考察了起始电压、电解时间、溶液初始p H对处理效果的影响。结果表明,在p H=7,U=14V、I=3.2A、t=30min的条件下,C.I.Reactiv...以C.I.Reactive Red 241、C.I.Disperse Blue 56模拟染料废水为对象,研究了电解法处理该类染料废水的优化条件。考察了起始电压、电解时间、溶液初始p H对处理效果的影响。结果表明,在p H=7,U=14V、I=3.2A、t=30min的条件下,C.I.Reactive Red241模拟染料废水的脱色率可达到86%以上;在p H=7,U=14V、I=3.2A、t=25min的条件下,C.I.Disperse Blue 56模拟染料废水的脱色率可达到79以上%。展开更多
The data obtained through this work revealed that the vermicompost is a natural adsorbent able to removal two textile dyes from an aqueous medium. The values of maximum adsorption capacity for congo red (23.25 mg/g) a...The data obtained through this work revealed that the vermicompost is a natural adsorbent able to removal two textile dyes from an aqueous medium. The values of maximum adsorption capacity for congo red (23.25 mg/g) and indigo blue (40.39 mg/g) obtained from the Mathematical Langmuir Model reveal it. The conditions of adsorbent mass, stirring time between adsorbent and dyes were optimized. Additionally, the values of Gibbs free energy demonstrate the predominance of physical interaction between both dyes and vermicompost. Through Langmuir constant values, it was possible to identify similar affinities between both dyes and vermicompost. The value of dimensionless constant indicates favorable adsorptions process. Finally, through physicochemical analysis from scanning electron microscopy and Fourier Transform Infra-Red Spectroscopy, the characteristics of vermicompost were verified revealing essential aspects to efficient adsorbent.展开更多
Optoelectronic applications require the development of new fluorescent and efficient luminescent materials, free of toxicity, low in cost, and easy to produce. In this way the synthesis of zinc-oxide (ZnO) quantum dot...Optoelectronic applications require the development of new fluorescent and efficient luminescent materials, free of toxicity, low in cost, and easy to produce. In this way the synthesis of zinc-oxide (ZnO) quantum dots (QDs) has recently received special attention due to their good optical, electrical and chemical properties with low production costs and blue light emission. In this work ZnO QDs were successfully doped with europium in order to obtain a tunable emission luminescence from blue emission of ZnO to red emission of europium as a function of wavelength excitation. Results show an efficient blue to red tuning when the excitation wavelength was changed from 317 nm to 395 nm, respectively. This opens the possibility of having new optical devices to produce different color emission using the same material.展开更多
This paper describes a brain-inspired simultaneous localization and mapping （SLAM） system using oriented features from accelerated segment test and rotated binary robust independent elementary （ORB） features of R...This paper describes a brain-inspired simultaneous localization and mapping （SLAM） system using oriented features from accelerated segment test and rotated binary robust independent elementary （ORB） features of RGB （red, green, blue） sensor for a mobile robot. The core SLAM system, dubbed RatSLAM, can construct a cognitive map using information of raw odometry and visual scenes in the path traveled. Different from existing RatSLAM system which only uses a simple vector to represent features of visual image, in this paper, we employ an efficient and very fast descriptor method, called ORB, to extract features from RCB images. Experiments show that these features are suitable to recognize the sequences of familiar visual scenes. Thus, while loop closure errors are detected, the descriptive features will help to modify the pose estimation by driving loop closure and localization in a map correction algorithm. Efficiency and robustness of our method are also demonstrated by comparing with different visual processing algorithms.展开更多
基金funded by the Scientific Research Fund of College of Science&Technology,Ningbo University for the Introduction of High-level Talents,China(RC190006)。
文摘Red and blue light illumination has been reported to significantly affect plantlet growth.Potato is an important food and feed crop in the world and potato plantlet cultured in vitro plays an important role in potato production.However,few studies have documented the effects of red and blue light on the growth of potato plantlets revealed at the transcriptome level.The objective of this study was to determine the growth and physiological responses of potato plantlets cultured in vitro under monochromatic red(RR),monochromatic blue(BB)as well as combined red and blue(RB)LEDs using the RNA-Seq technique.In total,3150 and 814 differentially expressed genes(DEGs)were detected in potato plantlets under RR and BB,respectively,compared to RB(used as control).Compared to the control,the DEGs enriched in"photosynthesis"and"photosynthesis-antenna proteins"metabolic pathways were up-regulated and down-regulated by BB and RR,respectively,which might be responsible for the increases and decreases of maximum quantum yield(F_(v)/F_(m)),photochemical quantum yield(φ_(PSII)),photochemical quenching(q_(P))and electron transfer rate(ETR)in BB and RR,respectively.Potato plantlets exhibited dwarfed stems and extended leaves under BB,whereas elongated stems and small leaves were induced under RR.These dramatically altered plantlet phenotypes were associated with variable levels of endogenous plant hormones gibberellin(GAs),indoleacetic acid(IAA)and cytokinins(CKs),as assessed in stems and leaves of potato plantlets.In addition,monochromatic red and blue LEDs trigged the opposite expression profiles of DEGs identified in the"plant hormone signal transduction"metabolic pathway,which were closely related to the endogenous plant hormone levels in potato plantlets.Our results provide insights into the responses of potato plantlets cultured in vitro to red and blue LEDs at the transcriptomic level and may contribute to improvements in the micro-propagation of potato plantlets cultured in vitro from the light spectrum aspect.
基金supported by the National Key Research and Development Program of China (2017YFD0701500)the Teamwork Projects Funded by Guangdong Natural Science Foundation, China (S2013030012842)the Guangzhou Science & Technology Project, China (201704020058)
文摘Lycopene, one of the strongest natural antioxidants known and the main carotene in ripe tomato, is very important for human health. Light is well known to be one of the most important environmental stimuli influencing lycopene biosynthesis; specifically, red light induces higher lycopene content in tomato. However, whether blue light promotes lycopene synthesis remains elusive and exactly how light stimulation promotes lycopene synthesis remains unclear. We applied supplemental blue and red lighting on tomato plants at anthesis to monitor the effect of supplemental blue and red lighting on lycopene synthesis. Our results showed that supplemental blue/red lighting induced higher lycopene content in tomato fruits; furthermore, we found that the expression of key genes in the lycopene synthesis pathway was induced by supplemented blue/red light. The expression of light signaling components, such as red-light receptor phytochromes(PHYs), blue-light receptor cryptochromes(CRYs) and light interaction factors, phytochrome-interacting factors(PIFs) and ELONGATED HYPOCOTYL 5(HY5) were up-or down-regulated by blue/red lighting. Thus, blue and red light increased lycopene content in tomatoes by inducing light receptors that modulate HY5 and PIFs activation to mediate phytoene synthase 1(PSY1) gene expression. These results provide a sound theoretical basis for further elucidation of the light regulating mechanism of lycopene synthesis in tomatoes, and for instituting a new generation of technological innovations for the enhancement of lycopene accumulation in crop production.
基金supported by National Natural Science Foundation of China (Grant No 50872091)the Natural Science Foundation of Tianjin,China(06YFJMJC02300,06TXTJJC14602)
文摘Fluorescence enhancement of red and blue concurrently emitting Ba3MgSi2O8:Eu2+,Mn2+ phosphors for plant cultivation has been investigated by Dy3+ addition.The Ba3MgSi2O8:Eu2+,Mn2+,Dy3+(BMS-EMD) phosphors have two-color emissions at the wavelength peak values of 437 nm and 620 nm at the excitation of 350 nm.The two emission bands are coincident with the absorption spectrum for photosynthesis of plants.An obvious enhancement effect has been observed upon addition of Dy3+ with amount of 0.03 mol%,in which the ...
基金Supported by Shanghai Science and Technology Commission Project,China(14DZ1206303)National Key Technology R&D Program(2014BAD05B05-05)Shanghai Agriculture Applied Technology Development Program,China(Grant No.20170201)
文摘Blue and red light are spectral wavelengths more effective for plants. The effects of different ratios of red and blue light （R/B=2, R/B=4, R/B=8, R/B=12） provided by LEDs on morphology and photosynthetic characteristics of tomato seedlings were studied. The results showed that plant height, stem diameter, fresh weight, dry weight, seedling index and G value increased with the increase of R/B ratio until 8. On the contrary, SPDA value decreased with the increase of R/B ratio. Photosynthetic characteristics were measured by CO 2 assimilation （ Pn ）, stomatal conductance （ gs ） and intracellular CO 2 concentration （ Ci） . Pn and gs decreased with the increase of R/B ratio. Furthermore, similar trend was investigated in photochemical quenching （qP） and electron transport rate （ETR）. Results of this study suggest that compared with white LED, appropriate combination of red and blue light can enhance plant growth and photosynthetic characteristics, and the optimal blue/red ratio for tomato growth was R/B=8.
基金Supported by the National Natural Science Foundation of China(No.41506188)the China Nantong Municipal Applied Basic Research Program(No.MS12017025-2)the Tianjin Demonstration Project for Innovative Development of Marine Economy(No.BHSF2017-21)
文摘The quality of light is an important abiotic factor that affects the growth and development of photosynthetic organisms.In this study,we exposed the unicellular green alga Dunaliella salina to red(660 nm)and blue(450 nm)light and analyzed the cell growth,total carotenoid content,and transcriptomes.The growth of D.salina was enhanced by illumination with red light,whereas blue light was not able to promote the algal growth.In contrast,the total carotenoid content increased under both red and blue light.The RNA of D.salina was sequenced and the transcriptomic response of algal cells to red and blue light was investigated.Six transcripts encoding for the blue light receptor cryptochrome were identified,and transcripts involved in the carotenoid metabolism were up-regulated under both red and blue light.Transcripts encoding for photoprotective enzymes related to the scavenging of reactive oxygen species were up-regulated under blue light.The present transcriptomic study provides a more comprehensive understanding of carotenoid biosynthesis in D.salina under different wavelengths of light.
文摘以C.I.Reactive Red 241、C.I.Disperse Blue 56模拟染料废水为对象,研究了电解法处理该类染料废水的优化条件。考察了起始电压、电解时间、溶液初始p H对处理效果的影响。结果表明,在p H=7,U=14V、I=3.2A、t=30min的条件下,C.I.Reactive Red241模拟染料废水的脱色率可达到86%以上;在p H=7,U=14V、I=3.2A、t=25min的条件下,C.I.Disperse Blue 56模拟染料废水的脱色率可达到79以上%。
文摘The data obtained through this work revealed that the vermicompost is a natural adsorbent able to removal two textile dyes from an aqueous medium. The values of maximum adsorption capacity for congo red (23.25 mg/g) and indigo blue (40.39 mg/g) obtained from the Mathematical Langmuir Model reveal it. The conditions of adsorbent mass, stirring time between adsorbent and dyes were optimized. Additionally, the values of Gibbs free energy demonstrate the predominance of physical interaction between both dyes and vermicompost. Through Langmuir constant values, it was possible to identify similar affinities between both dyes and vermicompost. The value of dimensionless constant indicates favorable adsorptions process. Finally, through physicochemical analysis from scanning electron microscopy and Fourier Transform Infra-Red Spectroscopy, the characteristics of vermicompost were verified revealing essential aspects to efficient adsorbent.
文摘Optoelectronic applications require the development of new fluorescent and efficient luminescent materials, free of toxicity, low in cost, and easy to produce. In this way the synthesis of zinc-oxide (ZnO) quantum dots (QDs) has recently received special attention due to their good optical, electrical and chemical properties with low production costs and blue light emission. In this work ZnO QDs were successfully doped with europium in order to obtain a tunable emission luminescence from blue emission of ZnO to red emission of europium as a function of wavelength excitation. Results show an efficient blue to red tuning when the excitation wavelength was changed from 317 nm to 395 nm, respectively. This opens the possibility of having new optical devices to produce different color emission using the same material.
基金supported by National Natural Science Foundation of China(No.61673283)
文摘This paper describes a brain-inspired simultaneous localization and mapping （SLAM） system using oriented features from accelerated segment test and rotated binary robust independent elementary （ORB） features of RGB （red, green, blue） sensor for a mobile robot. The core SLAM system, dubbed RatSLAM, can construct a cognitive map using information of raw odometry and visual scenes in the path traveled. Different from existing RatSLAM system which only uses a simple vector to represent features of visual image, in this paper, we employ an efficient and very fast descriptor method, called ORB, to extract features from RCB images. Experiments show that these features are suitable to recognize the sequences of familiar visual scenes. Thus, while loop closure errors are detected, the descriptive features will help to modify the pose estimation by driving loop closure and localization in a map correction algorithm. Efficiency and robustness of our method are also demonstrated by comparing with different visual processing algorithms.
