A recombinant skin model is a model in which skin cells are grown in vitro on a bioactive scaffold and provided with adequate nutrition to promote cell proliferation and differentiation to produce a mock skin structur...A recombinant skin model is a model in which skin cells are grown in vitro on a bioactive scaffold and provided with adequate nutrition to promote cell proliferation and differentiation to produce a mock skin structure and biological features. The development of recombinant skin models allows for effective and scientifically sound in vitro evaluation tests. This review briefly summarizes the overview of recombinant skin models and the progress of their application in in vitro evaluation which focuses on three aspects: skin irritation, skin corrosivity, and anti-skin aging. Moreover, an outlook on the future development of recombinant skin models is also provided in this review.展开更多
Thymidylate synthase （TS, E.C.2.1.1.45） catalyzes a critical reaction in the only pathway of de novo synthesis of thymidylate （dTMP） in human cells, and is an important target of chemotherapy. To evaluate the inhi...Thymidylate synthase （TS, E.C.2.1.1.45） catalyzes a critical reaction in the only pathway of de novo synthesis of thymidylate （dTMP） in human cells, and is an important target of chemotherapy. To evaluate the inhibitory activities of novel compounds to TS, a convenient method of activity assay using 6x His-tagged recombinant human TS （rhTS） was established and 49 novel synthetic folate analogues were screened to discover potential TS inhibitors. During the process, 4 novel compounds were found to effectively inhibit TS, while the IC 50 of a positive control raltitrexed was 3.4 μM in this assay.展开更多
We study the correlation between the trigger π and the associated J/ψ on near and away sides in Au＋Au collisions at sNN^（1/2） = 200 GeV. In the region of trigger momentum pt 〉 4 GeV/c, the π spectrum is compose...We study the correlation between the trigger π and the associated J/ψ on near and away sides in Au＋Au collisions at sNN^（1/2） = 200 GeV. In the region of trigger momentum pt 〉 4 GeV/c, the π spectrum is composed of thermal-shower and shower-shower recombinations in the frame work of the recombination model. We consider the azimuthal anisotropy in the quenched hard parton distribution and then calculate the elliptic flow parameter v2 of charmed mesons （J/ψ, D0 and Ds） for different centralities.展开更多
文摘A recombinant skin model is a model in which skin cells are grown in vitro on a bioactive scaffold and provided with adequate nutrition to promote cell proliferation and differentiation to produce a mock skin structure and biological features. The development of recombinant skin models allows for effective and scientifically sound in vitro evaluation tests. This review briefly summarizes the overview of recombinant skin models and the progress of their application in in vitro evaluation which focuses on three aspects: skin irritation, skin corrosivity, and anti-skin aging. Moreover, an outlook on the future development of recombinant skin models is also provided in this review.
基金supported by National Natural Science Foundation of China(Grant No.20972011,21042009,21172014)
文摘Thymidylate synthase （TS, E.C.2.1.1.45） catalyzes a critical reaction in the only pathway of de novo synthesis of thymidylate （dTMP） in human cells, and is an important target of chemotherapy. To evaluate the inhibitory activities of novel compounds to TS, a convenient method of activity assay using 6x His-tagged recombinant human TS （rhTS） was established and 49 novel synthetic folate analogues were screened to discover potential TS inhibitors. During the process, 4 novel compounds were found to effectively inhibit TS, while the IC 50 of a positive control raltitrexed was 3.4 μM in this assay.
基金Supported by National Natural Science Foundation of China (10635020, 10775057)Ministry of Education of China (306022)Project IRT0624 and Programme of Introducing Talents of Discipline to Universities (B08033)
文摘We study the correlation between the trigger π and the associated J/ψ on near and away sides in Au＋Au collisions at sNN^（1/2） = 200 GeV. In the region of trigger momentum pt 〉 4 GeV/c, the π spectrum is composed of thermal-shower and shower-shower recombinations in the frame work of the recombination model. We consider the azimuthal anisotropy in the quenched hard parton distribution and then calculate the elliptic flow parameter v2 of charmed mesons （J/ψ, D0 and Ds） for different centralities.
