Here we present a study on the genetic effects of agronomic and seed quality traits in double haploid （DH） populations, which were developed by microspore culture from reciprocal F1 hybrids produced between cultivar...Here we present a study on the genetic effects of agronomic and seed quality traits in double haploid （DH） populations, which were developed by microspore culture from reciprocal F1 hybrids produced between cultivar Californium and DH W-15. Each of the populations consisted of 25 DH lines. The field experiments were conducted in a randomized complete block design with three replications during three growing seasons. Field observations were recorded on seed yield and its structure： the number of branches and pods per plant, the number of seeds per pod, the 1 000-seed weight, the content of fat in the seeds, and three unsaturated fatty acids （oleic, linoleic, linolenic） in the seed oil. In order to investigate the influence of cross direction on the studied traits, parental effects were evaluated on the basis of differences between reciprocal DH populations. The maternal effect was revealed on the number of seeds per pod and the effect of the paternal form on linolenic acid content. The occurrence of transgression effects also depended on the direction of crossing and this was particularly noticeable in terms of the number of seeds per pod. The use of multivariate statistical methods allowed for the simultaneous characterization and grouping of tested lines in terms of several traits. Graphic images of the distribution of DH lines in the space of the two first canonical variates showed a great variation between the two reciprocal populations, both in terms of yield and its components, as well as fat and those unsaturated fatty acids.展开更多
Hybridization remains an important method for breeding new poplar varieties.It results in significant variation in leaf phenotype among parents and offspring,and among offspring themselves.This study aimed to investig...Hybridization remains an important method for breeding new poplar varieties.It results in significant variation in leaf phenotype among parents and offspring,and among offspring themselves.This study aimed to investigate whether leaf shape variations were similar in offspring produced from reciprocal crosses.Specifically,two hybrid combinations were produced:the direct cross with Populus ussuriensis as the maternal parent and P.simonii×P.nigra as the paternal parent(HY53),and the reciprocal cross with P.simonii×P.nigra as the maternal parent and P.ussuriensis as the paternal parent(HY268).Using 3-month-old rooted cuttings from 40 clones(36 F1 hybrids and their parents)growing in a greenhouse,we measured and analyzed 14 leaf morphological traits to assess genetic variation and heterosis.The results showed HY53 clones generally exhibited greater average height than HY268 clones.Leaf phenotypes differed between the two hybrid combinations,with significant differences observed among parents and offspring for almost all traits,as revealed by analysis of variance(ANOVA).The phenotypic coefficient of variation was higher in HY268 clones.Additionally,leaf traits demonstrated high repeatability.Notably,some hybrid offspring exhibited positive or negative mid-parent heterosis,as well as over-parent heterosis for certain leaf phenotypes.The systematic cluster analysis further indicated distinct separation among HY268 clones.This research provides valuable materials for poplar breeding and offers insights into hybrid vigor in wood plants.The findings highlight the importance of reciprocal crossing in influencing leaf phenotype variation and heterosis,offering practical insights for future breeding strategies.展开更多
The inheritance of chloroplast DNA (cpDNA) in sweet potato (Ipomoea batatas Lain.) was analyzed using DNA restriction fingerprinting. The cpDNA fingerprints of hybrids from reciprocal crosses between Xushu18 and AB78-...The inheritance of chloroplast DNA (cpDNA) in sweet potato (Ipomoea batatas Lain.) was analyzed using DNA restriction fingerprinting. The cpDNA fingerprints of hybrids from reciprocal crosses between Xushu18 and AB78-1 were found to be identical to those of their female parents, which reveals that cpDNA of sweet potato is maternally inherited in this intervarietal crossing. This maternal cpDNA transmission pattern does not accord with the putative one based on former cytological studies. The plastid inheritance in Convolvulaceae has been briefly reviewed in this study, and the utility of DNA restriction fingerprinting analysis in the study of plastid inheritance is also discussed.展开更多
The hybrid cotton varieties of Hanza 160 and Hanza 1692 were bred by reciprocal crosses between Han 5158 and Han 333 in Hainan and Hebei for several years. Both varieties had the characters of high yield, high quality...The hybrid cotton varieties of Hanza 160 and Hanza 1692 were bred by reciprocal crosses between Han 5158 and Han 333 in Hainan and Hebei for several years. Both varieties had the characters of high yield, high quality and disease resistance. Hanza 160 and Hanza 1692 were approved by Hebei Crop Variety Approval Committee in 2013 and 2016, respectively.展开更多
A laboratory colony of Coleomegilla maculata (De Geer) was selected for a novel phenotypic color trait. The phenotype was paler in color than the wild type, although not as pale as a previously described mutant strain...A laboratory colony of Coleomegilla maculata (De Geer) was selected for a novel phenotypic color trait. The phenotype was paler in color than the wild type, although not as pale as a previously described mutant strain, yellow (ye), and retained dark pigmentation in the eyes. This selected strain was named gold. Mendelian breeding experiments indicate a recessive biallelic inheritance. The strain has decreased fitness characteristics based on measurements of egg production and pupa size.展开更多
Tilapiine breeding success is season-dependent,with very rare exceptions.The female(f)to male(m)ratio differences showed significant variations in fruitful breeding.Furthermore,the female-to-male ratio in any hybridiz...Tilapiine breeding success is season-dependent,with very rare exceptions.The female(f)to male(m)ratio differences showed significant variations in fruitful breeding.Furthermore,the female-to-male ratio in any hybridization protocol involving reciprocal crosses may be the most influential factor in determining breeding seasonality.The innate genetic traits of tilapia,a prominent contributor to global aquaculture production,enable them to easily adapt to a wide range of aquatic environments,rendering them suitable for domestication.The study aimed to determine the breeding timeframe of Nile tilapia(Oreochromis niloticus,N)through interspecific monogamous/two-parent reciprocal crossing with Mozambique tilapia(O.mossambicus,M).Considering preselected genotypic characteristics by PrlLK microsatellite marker,different combinations of hybrid reciprocal crosses were performed in breeding hapa(3×3×3 cubic feet)inside an indoor plastered cemented tank and then in an outdoor earthen pond facility.Total 11 parental pairs were attempted to propagate inside a cemented tank with(9 pairs)and without(2 pairs)hapa during early April to mid-June.Unfortunately,the trials failed to produce any successful breeding within the schedule.Among those 11 pairs,6 pairs were Nf×Mm and 5 pairs were Mf×Nm cross.In the earthen pond inside hapa,a total of 18 pairs were attempted where seven breeding successes were reported without demonstrating any dependency for hybridizations over cross types or genotypes and for individuals over sex or species.Successful breeding were reported from the month of May to November.There were few overlapping months of successful breeding for different grouping categories.During the successful breeding period,there were no successful outcomes in both August and October.An inadvertent all-female F1 generation was observed for Mf×Nm crosses,while the reciprocal cross Nf×Mm produced an F1 generation with a nearly 1:1 female to male ratio.The F1 sex ratios exhibited a significant reliance on parental genotypic combinations(p=5.4e-11).The success stories from different cross combinations would help to frame the breeding protocol for future research.More precisely,for arranging a breeding schedule in freshwater earthen ponds inside hapa for a monogamous/two-parent hybrid cross the study light a close insight.展开更多
In sub-Saharan Africa cassava growing areas,two members of the Bemisia tabaci species complex termed sub-Saharan Africa 1(SSA1)and SSA2 have been reported as the prevalent whiteflies associated with the spread of viru...In sub-Saharan Africa cassava growing areas,two members of the Bemisia tabaci species complex termed sub-Saharan Africa 1(SSA1)and SSA2 have been reported as the prevalent whiteflies associated with the spread of viruses that cause cassava mosaic disease(CMD)and cassava brown streak disease(CBSD)pandemics.At the peak of CMD pandemic in the late 1990s,SSA2 was the prevalent whitefly,although its numbers have diminished over the last two decades with the resurgence of SSA1 whiteflies.Three SSA1 subgroups(SGI to SG3)are the predominant whiteflies in East Africa and vary in distribution and biological properties.Mating compatibility between SSA1 subgroups and SSA2 whiteflies was reported as the possible driver for the resurgence of SSA 1 whiteflies.In this study,a combination of both phylogenomic methods and reciprocal crossing experiments were applied to determine species status of SSA1 subgroups and SSA2 white-fly populations.Phylogenomic analyses conducted with 26548205 bp whole genome single nucleotide polymorphisms(SNPs)and the full mitogenomes clustered SSA1 subgroups together and separate from SSA2 species.Mating incompatibility between SSA1 subgroups and SSA2 further demonstrated their distinctiveness from each other.Phylogenomic analyses conducted with SNPs and mitogenomes also revealed different genetic relationships among SSA1 subgroups.The former clustered SSA 1-SGI and SSA1-SG2 to-gether but separate from SSA1-SG3,while the latter clustered SSA1-SG2 and SSA1-SG3 together but separate from SSA 1-SG1.Mating compatibility was observed between SSA 1-SGI and SSA1-SG2,while incompatibility occurred between SSA 1-SGI and SSA1-SG3,and SSA 1-SG2 and SSA 1-SG3.Mating results among SSA 1 subgroups were coherent with phylogenomics results based on SNPs but not the full mitogenomes.Furthermore,this study revealed that the secondary endosymbiont--did not mediate reproductive success in the crossing assays carried out.Overall,using genome wide SNPs together with reciprocal crossings assays,this study established accurate genetic relationships among cassava-colonizing populations,illustrating that SSA1 and SSA2 are distinct species while at least two species occur within SSA1 species.展开更多
文摘Here we present a study on the genetic effects of agronomic and seed quality traits in double haploid （DH） populations, which were developed by microspore culture from reciprocal F1 hybrids produced between cultivar Californium and DH W-15. Each of the populations consisted of 25 DH lines. The field experiments were conducted in a randomized complete block design with three replications during three growing seasons. Field observations were recorded on seed yield and its structure： the number of branches and pods per plant, the number of seeds per pod, the 1 000-seed weight, the content of fat in the seeds, and three unsaturated fatty acids （oleic, linoleic, linolenic） in the seed oil. In order to investigate the influence of cross direction on the studied traits, parental effects were evaluated on the basis of differences between reciprocal DH populations. The maternal effect was revealed on the number of seeds per pod and the effect of the paternal form on linolenic acid content. The occurrence of transgression effects also depended on the direction of crossing and this was particularly noticeable in terms of the number of seeds per pod. The use of multivariate statistical methods allowed for the simultaneous characterization and grouping of tested lines in terms of several traits. Graphic images of the distribution of DH lines in the space of the two first canonical variates showed a great variation between the two reciprocal populations, both in terms of yield and its components, as well as fat and those unsaturated fatty acids.
基金supported by“National Key R&D Program of China(2021YFD2200203)”“the Fundamental Research Funds for the Central Universities(No.2572022AW02 and No.2572023CT19)”。
文摘Hybridization remains an important method for breeding new poplar varieties.It results in significant variation in leaf phenotype among parents and offspring,and among offspring themselves.This study aimed to investigate whether leaf shape variations were similar in offspring produced from reciprocal crosses.Specifically,two hybrid combinations were produced:the direct cross with Populus ussuriensis as the maternal parent and P.simonii×P.nigra as the paternal parent(HY53),and the reciprocal cross with P.simonii×P.nigra as the maternal parent and P.ussuriensis as the paternal parent(HY268).Using 3-month-old rooted cuttings from 40 clones(36 F1 hybrids and their parents)growing in a greenhouse,we measured and analyzed 14 leaf morphological traits to assess genetic variation and heterosis.The results showed HY53 clones generally exhibited greater average height than HY268 clones.Leaf phenotypes differed between the two hybrid combinations,with significant differences observed among parents and offspring for almost all traits,as revealed by analysis of variance(ANOVA).The phenotypic coefficient of variation was higher in HY268 clones.Additionally,leaf traits demonstrated high repeatability.Notably,some hybrid offspring exhibited positive or negative mid-parent heterosis,as well as over-parent heterosis for certain leaf phenotypes.The systematic cluster analysis further indicated distinct separation among HY268 clones.This research provides valuable materials for poplar breeding and offers insights into hybrid vigor in wood plants.The findings highlight the importance of reciprocal crossing in influencing leaf phenotype variation and heterosis,offering practical insights for future breeding strategies.
文摘The inheritance of chloroplast DNA (cpDNA) in sweet potato (Ipomoea batatas Lain.) was analyzed using DNA restriction fingerprinting. The cpDNA fingerprints of hybrids from reciprocal crosses between Xushu18 and AB78-1 were found to be identical to those of their female parents, which reveals that cpDNA of sweet potato is maternally inherited in this intervarietal crossing. This maternal cpDNA transmission pattern does not accord with the putative one based on former cytological studies. The plastid inheritance in Convolvulaceae has been briefly reviewed in this study, and the utility of DNA restriction fingerprinting analysis in the study of plastid inheritance is also discussed.
基金Supported by the Key Technology R&D Program of Hebei Province(16226307D-4)
文摘The hybrid cotton varieties of Hanza 160 and Hanza 1692 were bred by reciprocal crosses between Han 5158 and Han 333 in Hainan and Hebei for several years. Both varieties had the characters of high yield, high quality and disease resistance. Hanza 160 and Hanza 1692 were approved by Hebei Crop Variety Approval Committee in 2013 and 2016, respectively.
文摘A laboratory colony of Coleomegilla maculata (De Geer) was selected for a novel phenotypic color trait. The phenotype was paler in color than the wild type, although not as pale as a previously described mutant strain, yellow (ye), and retained dark pigmentation in the eyes. This selected strain was named gold. Mendelian breeding experiments indicate a recessive biallelic inheritance. The strain has decreased fitness characteristics based on measurements of egg production and pupa size.
基金supported by a grant of BAS-USDA Program Project(BAS-USDA-PALS-BAU–FI–43),Bangladesh to M Sadiqul Islam.
文摘Tilapiine breeding success is season-dependent,with very rare exceptions.The female(f)to male(m)ratio differences showed significant variations in fruitful breeding.Furthermore,the female-to-male ratio in any hybridization protocol involving reciprocal crosses may be the most influential factor in determining breeding seasonality.The innate genetic traits of tilapia,a prominent contributor to global aquaculture production,enable them to easily adapt to a wide range of aquatic environments,rendering them suitable for domestication.The study aimed to determine the breeding timeframe of Nile tilapia(Oreochromis niloticus,N)through interspecific monogamous/two-parent reciprocal crossing with Mozambique tilapia(O.mossambicus,M).Considering preselected genotypic characteristics by PrlLK microsatellite marker,different combinations of hybrid reciprocal crosses were performed in breeding hapa(3×3×3 cubic feet)inside an indoor plastered cemented tank and then in an outdoor earthen pond facility.Total 11 parental pairs were attempted to propagate inside a cemented tank with(9 pairs)and without(2 pairs)hapa during early April to mid-June.Unfortunately,the trials failed to produce any successful breeding within the schedule.Among those 11 pairs,6 pairs were Nf×Mm and 5 pairs were Mf×Nm cross.In the earthen pond inside hapa,a total of 18 pairs were attempted where seven breeding successes were reported without demonstrating any dependency for hybridizations over cross types or genotypes and for individuals over sex or species.Successful breeding were reported from the month of May to November.There were few overlapping months of successful breeding for different grouping categories.During the successful breeding period,there were no successful outcomes in both August and October.An inadvertent all-female F1 generation was observed for Mf×Nm crosses,while the reciprocal cross Nf×Mm produced an F1 generation with a nearly 1:1 female to male ratio.The F1 sex ratios exhibited a significant reliance on parental genotypic combinations(p=5.4e-11).The success stories from different cross combinations would help to frame the breeding protocol for future research.More precisely,for arranging a breeding schedule in freshwater earthen ponds inside hapa for a monogamous/two-parent hybrid cross the study light a close insight.
基金supported by two grants obtained from the Bill&Melinda Gates FoundationEnabling Research Tools for Cassava Virologists and Breeders,OPP1080766the African Cassava Whitefly Project,OPP1058938.
文摘In sub-Saharan Africa cassava growing areas,two members of the Bemisia tabaci species complex termed sub-Saharan Africa 1(SSA1)and SSA2 have been reported as the prevalent whiteflies associated with the spread of viruses that cause cassava mosaic disease(CMD)and cassava brown streak disease(CBSD)pandemics.At the peak of CMD pandemic in the late 1990s,SSA2 was the prevalent whitefly,although its numbers have diminished over the last two decades with the resurgence of SSA1 whiteflies.Three SSA1 subgroups(SGI to SG3)are the predominant whiteflies in East Africa and vary in distribution and biological properties.Mating compatibility between SSA1 subgroups and SSA2 whiteflies was reported as the possible driver for the resurgence of SSA 1 whiteflies.In this study,a combination of both phylogenomic methods and reciprocal crossing experiments were applied to determine species status of SSA1 subgroups and SSA2 white-fly populations.Phylogenomic analyses conducted with 26548205 bp whole genome single nucleotide polymorphisms(SNPs)and the full mitogenomes clustered SSA1 subgroups together and separate from SSA2 species.Mating incompatibility between SSA1 subgroups and SSA2 further demonstrated their distinctiveness from each other.Phylogenomic analyses conducted with SNPs and mitogenomes also revealed different genetic relationships among SSA1 subgroups.The former clustered SSA 1-SGI and SSA1-SG2 to-gether but separate from SSA1-SG3,while the latter clustered SSA1-SG2 and SSA1-SG3 together but separate from SSA 1-SG1.Mating compatibility was observed between SSA 1-SGI and SSA1-SG2,while incompatibility occurred between SSA 1-SGI and SSA1-SG3,and SSA 1-SG2 and SSA 1-SG3.Mating results among SSA 1 subgroups were coherent with phylogenomics results based on SNPs but not the full mitogenomes.Furthermore,this study revealed that the secondary endosymbiont--did not mediate reproductive success in the crossing assays carried out.Overall,using genome wide SNPs together with reciprocal crossings assays,this study established accurate genetic relationships among cassava-colonizing populations,illustrating that SSA1 and SSA2 are distinct species while at least two species occur within SSA1 species.
