The canon of Chinese literature is so huge and intimidating that it’s hard to know where to start. And thanks to the difficulty of the Chinese language, only the most serious scholars can read the classics in their o...The canon of Chinese literature is so huge and intimidating that it’s hard to know where to start. And thanks to the difficulty of the Chinese language, only the most serious scholars can read the classics in their original form.Luckily there are some excellent English translations of great Chinese novels,offering you the chance to widen your reading horizons and learn about the culture of the country you’re living in.Here are five classics展开更多
Long-range sequencing grants insight into additional genetic information beyond what can be accessed by both short reads and modern longread technology.Several new sequencing technologies,such as“Hi-C”and“Linked Re...Long-range sequencing grants insight into additional genetic information beyond what can be accessed by both short reads and modern longread technology.Several new sequencing technologies,such as“Hi-C”and“Linked Reads”,produce long-range datasets for high-throughput and high-resolution genome analyses,which are rapidly advancing the field of genome assembly,genome scaffolding,and more comprehensive variant identification.In this review,we focused on five major long-range sequencing technologies:high-throughput chromosome conformation capture(Hi-C),10X Genomics Linked Reads,haplotagging,transposase enzyme linked long-read sequencing(TELL-seq),and singletube long fragment read(stLFR).We detailed the mechanisms and data products of the five platforms and their important applications,evaluated the quality of sequencing data from different platforms,and discussed the currently available bioinformatics tools.This work will benefit the selection of appropriate long-range technology for specific biological studies.展开更多
English reading proficiency is essential for university students in a globalized academic environment,yet many L2 learners encounter challenges,such as limited vocabulary,complex syntax,and unclear text organization,l...English reading proficiency is essential for university students in a globalized academic environment,yet many L2 learners encounter challenges,such as limited vocabulary,complex syntax,and unclear text organization,leading to cognitive overload.Grounded in the Cognitive Load Theory(CLT),this study examines the role of the Chunking Reading Processing Strategy-which integrates fragmented linguistic information into meaningful units at lexical,syntactic,and discourse levels-in alleviating cognitive load and improving reading comprehension.Through a mixed-methods approach,the research investigates how learners at different proficiency levels perceive and apply the chunking strategy,and how such application relates to cognitive load management.The results indicate that higher-proficiency learners employ chunking more frequently and report greater benefits,whereas lower-proficiency learners depend more on instructional support.The study confirms the theoretical and pedagogical value of chunk-based reading instruction and suggests that differentiated,cognitively informed teaching of the chunking strategy can enhance both reading efficiency and strategic awareness among L2 learners.展开更多
As a public basic course and the core general course of undergraduate education,moral education(ME)in College English is imperative.It is necessary to design specific teaching programs flexibly and creatively on the b...As a public basic course and the core general course of undergraduate education,moral education(ME)in College English is imperative.It is necessary to design specific teaching programs flexibly and creatively on the basis of fully understanding the ideas,objectives and tasks,basic principles and implementation methods of ME.This paper tries integrating literature reading into unit topic teaching as a new approach to ME in College English.The specific pathways of integration include:expansion of literary reading content,diversified teaching of literary reading,diversified media of literary reading,and diversified assessment system of literary quality.The integration of literature reading and unit topic teaching is conducive to the realization of the teaching goal of cultivating students’language ability,humanistic quality,and correct values.展开更多
The accuracy of genomic annotation is crucial for subsequent functional investigations;however,computational protocols used in high-throughput annotation of open reading frames(ORFs)can introduce inconsistencies.These...The accuracy of genomic annotation is crucial for subsequent functional investigations;however,computational protocols used in high-throughput annotation of open reading frames(ORFs)can introduce inconsistencies.These inconsistencies,which lead to non-uniform extension or truncation of sequence ends,pose challenges for downstream analyses.Existing strategies to rectify these inconsistencies are time-consuming and labor-intensive,lacking specific approaches.To address this gap,we developed to GC,a tool that integrates genomic annotation with RNA-seq datasets to rectify annotation inconsistencies.Using to GC,we achieved an accuracy of nearly 100%accuracy in correcting inconsistencies in published Phytophthora sojae ORFs.We applied this innovative pipeline to the GPCR-bigrams gene family,which was predicted to have 42 members in the P.sojae genome but lacked experimental validation.By employing to GC,we identified 32 GPCR-bigram ORFs with inconsistencies between previous annotations and to GC-corrected sequences.Notably,among these were 5 genes(GPCR-TKL9,GPCR-TKL15,GPCR-PDE3,GPCR-AC3,and GPCR-AC4)showed substantial inconsistencies.Experimental gene annotation confirmed the effectiveness of to GC,as sequences obtained through cloning matched those annotated by to GC.Importantly,we discovered two novel GPCRs(GPCR-AC3 and GPCR-AC4),which were previously mispredicted as a single gene.CRISPR/Cas9-mediated knockout experiments revealed the involvement of GPCR-AC4 but not GPCR-AC3 in oospore production,further confirming their status as two separate genes.In addition to P.sojae,the reliability of the to GC pipeline in Phytophthora capsici and Pythium ultimum further emphasizes the robustness of this pipeline.Our findings highlight the utility of to GC for reliable gene model correction,facilitating investigations into biological functions and offering potential applications in diverse species analyses.展开更多
To enhance the friendship between China and Laos and jointly advance the cause of poverty reduction,the Publicity Department of the Communist Party of China (CPC) Yunnan Provincial Committee,the Center for Asia-Pacifi...To enhance the friendship between China and Laos and jointly advance the cause of poverty reduction,the Publicity Department of the Communist Party of China (CPC) Yunnan Provincial Committee,the Center for Asia-Pacific of China International Communications Group (CICG AsiaPacific), and the Lao newspaper Pasaxon (The People) co-hosted a reading club event themed “Up and Out of Poverty.” Organized by the Yunnan International Communication Center for South and Southeast Asia,the event took place at the National Library of Laos on February 6.展开更多
聚焦PEP新教材Start to read板块教学,针对传统阅读启蒙课存在的思维培养不足、教学活动单一等问题,提出“问题·活动·评价”三位一体的教学新范式。通过构建层级化问题体系引导思维进阶,设计梯度式活动促进语篇理解,实施全程...聚焦PEP新教材Start to read板块教学,针对传统阅读启蒙课存在的思维培养不足、教学活动单一等问题,提出“问题·活动·评价”三位一体的教学新范式。通过构建层级化问题体系引导思维进阶,设计梯度式活动促进语篇理解,实施全程化评价保障学习效果。研究表明,该范式能有效提升学生阅读兴趣与综合语言能力,为小学英语阅读启蒙教学提供可复制、可推广的创新路径。展开更多
With the support by the National Natural Science Foundation of China,Prof.Ruan Jue(阮珏)at the Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences,developed a novel long reads assemble...With the support by the National Natural Science Foundation of China,Prof.Ruan Jue(阮珏)at the Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences,developed a novel long reads assembler,which was published in Nature Methods(2020,17:155—158).展开更多
Novel insights into complex biological processes very often critically depend on the establishment of new potent read-out tools and improved protocols.A lot has been learned over the past four decades on physiological...Novel insights into complex biological processes very often critically depend on the establishment of new potent read-out tools and improved protocols.A lot has been learned over the past four decades on physiological functions and,importantly,disease-related roles of the prion protein(PrP),a relatively broadly expressed membrane-anchored glycoprotein with high levels in several cell types of the nervous and immune system and with well-established key roles in different progressive and fatal neurodegenerative protein misfolding diseases(proteopathies).展开更多
Many people have bad habits,and they are not good for you.Bad habits can be staying up too late,eating too many sweets or biting your nails.Bad habits aren't easy to break,but you can do it!First,notice when you d...Many people have bad habits,and they are not good for you.Bad habits can be staying up too late,eating too many sweets or biting your nails.Bad habits aren't easy to break,but you can do it!First,notice when you do the habit.Then,try to do something else,such as chewing gum or reading a book.Ask a friend to help you stop.They can watch you and remind you.Do your best and don't stop trying.Changing little by little can make a difference!展开更多
The high-fidelity(HiFi)long-read sequencing technology developed by PacBio has greatly improved the base-level accuracy of genome assemblies.However,these assemblies still contain base-level errors,particularly within...The high-fidelity(HiFi)long-read sequencing technology developed by PacBio has greatly improved the base-level accuracy of genome assemblies.However,these assemblies still contain base-level errors,particularly within the error-prone regions of HiFi long reads.Existing genome polishing tools usually introduce overcorrections and haplotype switch errors when correcting errors in genomes assembled from HiFi long reads.Here,we describe an upgraded genome polishing tool-NextPolish2,which can fix base errors remaining in those“highly accurate”genomes assembled from HiFi long reads without introducing excessive overcorrections and haplotype switch errors.We believe that NextPolish2 has a great significance to further improve the accuracy of telomere-to-telomere(T2T)genomes.NextPolish2 is freely available at https://github.com/Nextomics/NextPolish2.展开更多
My mother is a worker in a factory and she is very busy every day.After got1up at six o'clock,she brushes her teeth and washes her face.Then she does breakfast2for us.She often has breakfast at half past six and s...My mother is a worker in a factory and she is very busy every day.After got1up at six o'clock,she brushes her teeth and washes her face.Then she does breakfast2for us.She often has breakfast at half past six and she goes to work at seven.She works for eight hours every day.She often gets home at six thirty in the evening.Then she cooks dinner for us.After dinner,she watches TV or reads books for a while.Sometimes she helps me with my homework.She usually goes to bed about3twenty to eleven.展开更多
In this data explosion era,ensuring the secure storage,access,and transmission of information is imperative,encom-passing all aspects ranging from safeguarding personal devices to formulating national information secu...In this data explosion era,ensuring the secure storage,access,and transmission of information is imperative,encom-passing all aspects ranging from safeguarding personal devices to formulating national information security strategies.Leverag-ing the potential offered by dual-type carriers for transportation and employing optical modulation techniques to develop high reconfigurable ambipolar optoelectronic transistors enables effective implementation of information destruction after read-ing,thereby guaranteeing data security.In this study,a reconfigurable ambipolar optoelectronic synaptic transistor based on poly(3-hexylthiophene)(P3HT)and poly[[N,N-bis(2-octyldodecyl)-napthalene-1,4,5,8-bis(dicarboximide)-2,6-diyl]-alt-5,5′-(2,2′-bithiophene)](N2200)blend film was fabricated through solution-processed method.The resulting transistor exhib-ited a relatively large ON/OFF ratio of 10^(3) in both n-and p-type regions,and tunable photoconductivity after light illumination,particularly with green light.The photo-generated carriers could be effectively trapped under the gate bias,indicating its poten-tial application in mimicking synaptic behaviors.Furthermore,the synaptic plasticity,including volatile/non-volatile and excita-tory/inhibitory characteristics,could be finely modulated by electrical and optical stimuli.These optoelectronic reconfigurable properties enable the realization of information light assisted burn after reading.This study not only offers valuable insights for the advancement of high-performance ambipolar organic optoelectronic synaptic transistors but also presents innovative ideas for the future information security access systems.展开更多
Electromagnetic technology used in logging while drilling(LWD) provides the resistivity distribution around a borehole within a range of several tens of meters.However,a blind zone appears in front of the drill bit wh...Electromagnetic technology used in logging while drilling(LWD) provides the resistivity distribution around a borehole within a range of several tens of meters.However,a blind zone appears in front of the drill bit when operating in high-angle wells,limiting the ability to detect formations ahead of the drill bit.Look-ahead technology addresses this issue and substantially enhances the proactive capability of geological directional drilling.In this study,we examine the detection capabilities of various component combinations of magnetic dipole antenna.Based on the sensitivity of each component to the axial information,a coaxial component is selected as a boundary indicator.We investigate the impact of various factors,such as frequency and transmitter and receiver(TR) distance,under different geological models.This study proposes 5 and 20 kHz as appropriate frequencies,and 10-14 and 12-17 m as suitable TR distance combinations.The accuracy of the numerical calculation results is verified via air-sea testing,confirming the instrument's detection capability.A test model that eliminated the influence of environmental factors and seawater depth is developed.The results have demonstrated that the tool can recognize the interface between layers up to 21.6 m ahead.It provides a validation idea for the design of new instruments as well as the validation of detection capabilities.展开更多
文摘The canon of Chinese literature is so huge and intimidating that it’s hard to know where to start. And thanks to the difficulty of the Chinese language, only the most serious scholars can read the classics in their original form.Luckily there are some excellent English translations of great Chinese novels,offering you the chance to widen your reading horizons and learn about the culture of the country you’re living in.Here are five classics
基金supported by from the National Natural Science Foundation of China(Grant No.32070601)the Natural Science Fund for Excellent Young Scholars of Shandong Province,China(Grant No.ZR2022YQ23)supported by the Wellcome Trust,UK(Grant No.WT206194).
文摘Long-range sequencing grants insight into additional genetic information beyond what can be accessed by both short reads and modern longread technology.Several new sequencing technologies,such as“Hi-C”and“Linked Reads”,produce long-range datasets for high-throughput and high-resolution genome analyses,which are rapidly advancing the field of genome assembly,genome scaffolding,and more comprehensive variant identification.In this review,we focused on five major long-range sequencing technologies:high-throughput chromosome conformation capture(Hi-C),10X Genomics Linked Reads,haplotagging,transposase enzyme linked long-read sequencing(TELL-seq),and singletube long fragment read(stLFR).We detailed the mechanisms and data products of the five platforms and their important applications,evaluated the quality of sequencing data from different platforms,and discussed the currently available bioinformatics tools.This work will benefit the selection of appropriate long-range technology for specific biological studies.
基金funded by the 2024 Shanghai Social Science Planning Annual Project titled“A Study on the Chunk Processing Mechanisms and Cognitive Motivations of ESL Reading”(Fund No.2024BYY012).
文摘English reading proficiency is essential for university students in a globalized academic environment,yet many L2 learners encounter challenges,such as limited vocabulary,complex syntax,and unclear text organization,leading to cognitive overload.Grounded in the Cognitive Load Theory(CLT),this study examines the role of the Chunking Reading Processing Strategy-which integrates fragmented linguistic information into meaningful units at lexical,syntactic,and discourse levels-in alleviating cognitive load and improving reading comprehension.Through a mixed-methods approach,the research investigates how learners at different proficiency levels perceive and apply the chunking strategy,and how such application relates to cognitive load management.The results indicate that higher-proficiency learners employ chunking more frequently and report greater benefits,whereas lower-proficiency learners depend more on instructional support.The study confirms the theoretical and pedagogical value of chunk-based reading instruction and suggests that differentiated,cognitively informed teaching of the chunking strategy can enhance both reading efficiency and strategic awareness among L2 learners.
文摘As a public basic course and the core general course of undergraduate education,moral education(ME)in College English is imperative.It is necessary to design specific teaching programs flexibly and creatively on the basis of fully understanding the ideas,objectives and tasks,basic principles and implementation methods of ME.This paper tries integrating literature reading into unit topic teaching as a new approach to ME in College English.The specific pathways of integration include:expansion of literary reading content,diversified teaching of literary reading,diversified media of literary reading,and diversified assessment system of literary quality.The integration of literature reading and unit topic teaching is conducive to the realization of the teaching goal of cultivating students’language ability,humanistic quality,and correct values.
基金supported by the grants to Min Qiu and Ming Wang from the National Natural Science Foundation of China(32100160 and 32100044)the grants to Ming Wang from the Jiangsu“Innovative and Entrepreneurial Talent”Program,China(JSSCRC2021510)the grants to Yuanchao Wang from the Chinese Modern Agricultural Industry Technology System(CARS-004-PS14)。
文摘The accuracy of genomic annotation is crucial for subsequent functional investigations;however,computational protocols used in high-throughput annotation of open reading frames(ORFs)can introduce inconsistencies.These inconsistencies,which lead to non-uniform extension or truncation of sequence ends,pose challenges for downstream analyses.Existing strategies to rectify these inconsistencies are time-consuming and labor-intensive,lacking specific approaches.To address this gap,we developed to GC,a tool that integrates genomic annotation with RNA-seq datasets to rectify annotation inconsistencies.Using to GC,we achieved an accuracy of nearly 100%accuracy in correcting inconsistencies in published Phytophthora sojae ORFs.We applied this innovative pipeline to the GPCR-bigrams gene family,which was predicted to have 42 members in the P.sojae genome but lacked experimental validation.By employing to GC,we identified 32 GPCR-bigram ORFs with inconsistencies between previous annotations and to GC-corrected sequences.Notably,among these were 5 genes(GPCR-TKL9,GPCR-TKL15,GPCR-PDE3,GPCR-AC3,and GPCR-AC4)showed substantial inconsistencies.Experimental gene annotation confirmed the effectiveness of to GC,as sequences obtained through cloning matched those annotated by to GC.Importantly,we discovered two novel GPCRs(GPCR-AC3 and GPCR-AC4),which were previously mispredicted as a single gene.CRISPR/Cas9-mediated knockout experiments revealed the involvement of GPCR-AC4 but not GPCR-AC3 in oospore production,further confirming their status as two separate genes.In addition to P.sojae,the reliability of the to GC pipeline in Phytophthora capsici and Pythium ultimum further emphasizes the robustness of this pipeline.Our findings highlight the utility of to GC for reliable gene model correction,facilitating investigations into biological functions and offering potential applications in diverse species analyses.
文摘To enhance the friendship between China and Laos and jointly advance the cause of poverty reduction,the Publicity Department of the Communist Party of China (CPC) Yunnan Provincial Committee,the Center for Asia-Pacific of China International Communications Group (CICG AsiaPacific), and the Lao newspaper Pasaxon (The People) co-hosted a reading club event themed “Up and Out of Poverty.” Organized by the Yunnan International Communication Center for South and Southeast Asia,the event took place at the National Library of Laos on February 6.
文摘聚焦PEP新教材Start to read板块教学,针对传统阅读启蒙课存在的思维培养不足、教学活动单一等问题,提出“问题·活动·评价”三位一体的教学新范式。通过构建层级化问题体系引导思维进阶,设计梯度式活动促进语篇理解,实施全程化评价保障学习效果。研究表明,该范式能有效提升学生阅读兴趣与综合语言能力,为小学英语阅读启蒙教学提供可复制、可推广的创新路径。
文摘With the support by the National Natural Science Foundation of China,Prof.Ruan Jue(阮珏)at the Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences,developed a novel long reads assembler,which was published in Nature Methods(2020,17:155—158).
基金supported by the CJD Foundation,USA,the Alzheimer Forschung Initiative(AFI)e.V.,Germany,and Werner-Otto-Stiftung,Germany(all to HCA),ChinaScholarship Council(grant#202108080249 to FS)Deutsche Forschungsgemeinschaft(DFG)CRC877“Proteolysis as a regulatory event in pathophysiology”(project A12 to MG),Slovene Research and InnovationAgency(grant number P4-0176 to VCS).
文摘Novel insights into complex biological processes very often critically depend on the establishment of new potent read-out tools and improved protocols.A lot has been learned over the past four decades on physiological functions and,importantly,disease-related roles of the prion protein(PrP),a relatively broadly expressed membrane-anchored glycoprotein with high levels in several cell types of the nervous and immune system and with well-established key roles in different progressive and fatal neurodegenerative protein misfolding diseases(proteopathies).
文摘Many people have bad habits,and they are not good for you.Bad habits can be staying up too late,eating too many sweets or biting your nails.Bad habits aren't easy to break,but you can do it!First,notice when you do the habit.Then,try to do something else,such as chewing gum or reading a book.Ask a friend to help you stop.They can watch you and remind you.Do your best and don't stop trying.Changing little by little can make a difference!
基金supported by the National Key R&D Program of China(Grant No.2022YFC3400300)the National Natural Science Foundation of China(Grant Nos.32125009 and 32070663).
文摘The high-fidelity(HiFi)long-read sequencing technology developed by PacBio has greatly improved the base-level accuracy of genome assemblies.However,these assemblies still contain base-level errors,particularly within the error-prone regions of HiFi long reads.Existing genome polishing tools usually introduce overcorrections and haplotype switch errors when correcting errors in genomes assembled from HiFi long reads.Here,we describe an upgraded genome polishing tool-NextPolish2,which can fix base errors remaining in those“highly accurate”genomes assembled from HiFi long reads without introducing excessive overcorrections and haplotype switch errors.We believe that NextPolish2 has a great significance to further improve the accuracy of telomere-to-telomere(T2T)genomes.NextPolish2 is freely available at https://github.com/Nextomics/NextPolish2.
文摘My mother is a worker in a factory and she is very busy every day.After got1up at six o'clock,she brushes her teeth and washes her face.Then she does breakfast2for us.She often has breakfast at half past six and she goes to work at seven.She works for eight hours every day.She often gets home at six thirty in the evening.Then she cooks dinner for us.After dinner,she watches TV or reads books for a while.Sometimes she helps me with my homework.She usually goes to bed about3twenty to eleven.
基金the National Natural-Science Foundation of China(Grant No.62304137)Guangdong Basic and Applied Basic Research Foundation(Grant Nos.2023A1515012479,2024A1515011737,and 2024A1515010006)+4 种基金the Science and Technology Innovation Commission of Shenzhen(Grant No.JCYJ20220818100206013)RSC Researcher Collaborations Grant(Grant No.C23-2422436283)State Key Laboratory of Radio Frequency Heterogeneous Integration(Independent Scientific Research Program No.2024010)the Project on Frontier and Interdisciplinary Research Assessment,Academic Divisions of the Chinese Academy of Sciences(Grant No.XK2023XXA002)NTUT-SZU Joint Research Program.
文摘In this data explosion era,ensuring the secure storage,access,and transmission of information is imperative,encom-passing all aspects ranging from safeguarding personal devices to formulating national information security strategies.Leverag-ing the potential offered by dual-type carriers for transportation and employing optical modulation techniques to develop high reconfigurable ambipolar optoelectronic transistors enables effective implementation of information destruction after read-ing,thereby guaranteeing data security.In this study,a reconfigurable ambipolar optoelectronic synaptic transistor based on poly(3-hexylthiophene)(P3HT)and poly[[N,N-bis(2-octyldodecyl)-napthalene-1,4,5,8-bis(dicarboximide)-2,6-diyl]-alt-5,5′-(2,2′-bithiophene)](N2200)blend film was fabricated through solution-processed method.The resulting transistor exhib-ited a relatively large ON/OFF ratio of 10^(3) in both n-and p-type regions,and tunable photoconductivity after light illumination,particularly with green light.The photo-generated carriers could be effectively trapped under the gate bias,indicating its poten-tial application in mimicking synaptic behaviors.Furthermore,the synaptic plasticity,including volatile/non-volatile and excita-tory/inhibitory characteristics,could be finely modulated by electrical and optical stimuli.These optoelectronic reconfigurable properties enable the realization of information light assisted burn after reading.This study not only offers valuable insights for the advancement of high-performance ambipolar organic optoelectronic synaptic transistors but also presents innovative ideas for the future information security access systems.
基金co-funded by the National Key Research and Development Program of China under Grant (2019YFA0708301)the CAS Project for Young Scientists in Basic Research (Grant No.YSBR-082)Research Instrument and Equipment Development Project of Chinese Academy of Sciences (GJJSTD20210008)。
文摘Electromagnetic technology used in logging while drilling(LWD) provides the resistivity distribution around a borehole within a range of several tens of meters.However,a blind zone appears in front of the drill bit when operating in high-angle wells,limiting the ability to detect formations ahead of the drill bit.Look-ahead technology addresses this issue and substantially enhances the proactive capability of geological directional drilling.In this study,we examine the detection capabilities of various component combinations of magnetic dipole antenna.Based on the sensitivity of each component to the axial information,a coaxial component is selected as a boundary indicator.We investigate the impact of various factors,such as frequency and transmitter and receiver(TR) distance,under different geological models.This study proposes 5 and 20 kHz as appropriate frequencies,and 10-14 and 12-17 m as suitable TR distance combinations.The accuracy of the numerical calculation results is verified via air-sea testing,confirming the instrument's detection capability.A test model that eliminated the influence of environmental factors and seawater depth is developed.The results have demonstrated that the tool can recognize the interface between layers up to 21.6 m ahead.It provides a validation idea for the design of new instruments as well as the validation of detection capabilities.
