Reader proteins that bind specific methyllysine are important to biological functions of lysine methylation,but readers of many methyllysine sites are still unknown.Therefore,development of covalent probes is importan...Reader proteins that bind specific methyllysine are important to biological functions of lysine methylation,but readers of many methyllysine sites are still unknown.Therefore,development of covalent probes is important to identify readers from cell samples so as to understand biological roles of lysine methylation.Generally,readers bind methyllysine via aromatic cages that contain tryptophan,tyrosine and phenylalanine,that offer a unique motif for selective crosslinking.We recently reported a site-selective tryptophan crosslinking strategy based on dimethylsulfonium that mimics dimethyllysine to crosslink tryptophan in aromatic cages of readers.Since tyrosine is a key residue for binding affinity to methyllysine,especially some readers that do not contain tryptophan residues in the binding pocket.Here we developed strategies of site-selective crosslinking to tyrosine.Ultraviolet(UV)source was applied to excite tyrosine at neutral pH or phenoxide at basic p H,and subsequent single-electron transfer(SET)from Tyr*to sulfonium inside the binding pocket enables selective crosslinking.In consequence,methyllysine readers with tyrosine-containing aromatic cages could be selectively crosslinked by site-specific sulfonium peptide probes.In addition,we expanded substrates from aromatic cages to tyrosine residues of proximate contact with sulfonium probes.The pair of LgBiT and SmBiT exhibited orthogonal crosslinking in complicated cell samples.As a result,we may expand sulfonium tools to target local tyrosine in future investigations.展开更多
In the context of the continuous deepening of the“Double Reduction”policy and the growing demand for quality education,leveled mathematics readers,as an emerging form of publishing that integrates subject education ...In the context of the continuous deepening of the“Double Reduction”policy and the growing demand for quality education,leveled mathematics readers,as an emerging form of publishing that integrates subject education and reading experience,face challenges such as unclear leveling logic,insufficient functional support,and weak user engagement.This paper introduces the 4V marketing theory and constructs an analytical framework from four dimensions:differentiation,functionality,added value,and resonance.Two representative products,“Climbing Mathematics”and“Spark Mathematics,”are selected for a typical case comparison to identify their strengths and weaknesses in content design,service systems,and brand operation,and to extract transferable strategic elements.The study finds that the user-value-oriented strategy based on the 4V model can effectively address the core issues in the market promotion and user relationship building of leveled mathematics readers,providing practical paths and theoretical support for educational publishing institutions to achieve product innovation and brand upgrading in this niche field.展开更多
The dissemination of Chinese culture is closely related to the influence of national culture.Chinese culture readers serve as important carriers for cultivating global competence among young people and disseminating C...The dissemination of Chinese culture is closely related to the influence of national culture.Chinese culture readers serve as important carriers for cultivating global competence among young people and disseminating Chinese culture.They are also important measures to help different cultures eliminate cultural barriers.However,the current Chinese culture readers are of various kinds,seldom testing the readability and rarely taking consideration of the target readers’accessibility.Therefore,this study explores the factors affecting the readability of different Chinese culture readers from the perspective of English global localization,aiming to explore the ways and methods to enhance the dissemination of Chinese culture.Meanwhile,it conducted a survey on the target readers’competence in language learning and culture awareness.展开更多
A quadrature mixer with a shared transconductor stage is analyzed,including voltage conversion gain, linearity, noise figure, and image rejection. The analysis indicates it has better performance than a conventional G...A quadrature mixer with a shared transconductor stage is analyzed,including voltage conversion gain, linearity, noise figure, and image rejection. The analysis indicates it has better performance than a conventional Gilbert mixer pair in commutating mode. A quadrature down-conversion mixer based on this topology is designed and optimized for an ultra high frequency RFID reader. Operating in the 915MHz ISM band, the presented quadrature mixer measures a conversion gain of 12.5dB,an IIP3 of 10dBm, an IIP2 of 58dBm, and an SSB noise figure of 17.6riB. The chip was fabricated in a 0. 18μm 1P6M RF CMOS process and consumes only 3mA of current from a 1.8V power supply.展开更多
AIM： To determine the performance of novice readers （4^th year medical students） for detecting capsule endoscopy findings. METHODS： Ten capsule endoscopy cases of small bowel lesions were administered to the reade...AIM： To determine the performance of novice readers （4^th year medical students） for detecting capsule endoscopy findings. METHODS： Ten capsule endoscopy cases of small bowel lesions were administered to the readers. Gold standard findings were pre-defined by gastroenterologists. Ten gold standard ＂targets＂ were identified among the 10 cases. Readers were given a 30-min overview of Rapid Reader software and instructed to mark any potential areas of abnormalities. A software program was developed using SAS to analyze the thumbnailed findings. RESULTS： The overall sensitivity for detecting the gold standard findings was 80%. As a group, at least 5 out of 10 readers detected each gold standard finding per recording. All the gold standard targets were identified when the readers＇ results were combined. Incidental finding/false positive rate ranged between 8.2-59.8 per reader. CONCLUSION： A panel of medical students with minimal endoscopic experience can achieve high sensitivity in detecting lesions on capsule endoscopy. A group of novice readers can pre-screen recordings to thumbnail potential areas of small bowel lesions for further review. These thumbnails must be reviewed to determine the clinical relevance. Further studies are ongoing to assess other cohorts.展开更多
Reading is a very essential skill in the process of knowledge aqusition,while,effective reading ability is the outcome of the good combination of both time and skills.The differences between good readers and poor read...Reading is a very essential skill in the process of knowledge aqusition,while,effective reading ability is the outcome of the good combination of both time and skills.The differences between good readers and poor reader will be studied in terms of comparisons towards their reading speed,efficiency and reasons behind in the domain of psychological perspectives.展开更多
This letter proposes updating the World Journal of Gastroenterology(WJG) with several new items and subsections and seeks the readers' input for these suggested changes. In order to further enhance the scope of th...This letter proposes updating the World Journal of Gastroenterology(WJG) with several new items and subsections and seeks the readers' input for these suggested changes. In order to further enhance the scope of the WJG, to make it more interactive, and to accommodate our readers' interest, the editors ask the readers for their input regarding potential new proposed changes and new subsections listed below:(1) new subsection-selected highlights from other related Baishideng Publishing Group world series journals;(2) new subsection-challenging images of the week-a quiz with an answer and references on another page;(3) to re-introduce or restore for some issues the cover page on which selected illustration;(4) new subsection-new hypotheses, new concepts and revisiting old concepts in a short, commentary format;(5) preview of the forthcoming issue articles(title and authors);(6) suggestions submitted by the readers-new topics and initiatives; and(7) additional topic area-molecular basis of gastric/liver diseases as an additional area for articles submitted to the journal.展开更多
Aims and Scope World Journal of Emergency Medicine(ISSN 1920-8642,CN 33-1408/R),a quarterly journal,publishes peer-reviewed original research articles,review articles including systematic review and meta-analysis,edit...Aims and Scope World Journal of Emergency Medicine(ISSN 1920-8642,CN 33-1408/R),a quarterly journal,publishes peer-reviewed original research articles,review articles including systematic review and meta-analysis,editorials,commentaries,etc in different fields of emergency medicine.The content of articles could be varied in medical,surgical,radiological,pathological,biochemical,physiological and pharmaceutical aspects of the subject areas.The journal also deals with basic sciences and experimental work.展开更多
Copyright 2013, Hepatobiliary & Pancreatic Diseases International. All rights reserved. www.hbpdint.com Aims and Scope Hepatobiliary & Pancreatic Diseases International publishes peer-reviewed original papers,...Copyright 2013, Hepatobiliary & Pancreatic Diseases International. All rights reserved. www.hbpdint.com Aims and Scope Hepatobiliary & Pancreatic Diseases International publishes peer-reviewed original papers, reviews (meta-analysis, systematic review) and editorials concerned with clinical practice and research in the fields of hepatobiliary and pancreatic展开更多
Aims and Scope Hepatobiliary & Pancreatic Diseases International publishes peer-reviewed original papers, reviews and editorials concerned with clinical practice and research in the fields of hepatobiliary and pan...Aims and Scope Hepatobiliary & Pancreatic Diseases International publishes peer-reviewed original papers, reviews and editorials concerned with clinical practice and research in the fields of hepatobiliary and pancreatic diseases. Papers cover the medical, surgical, radiological, pathological, biochemical , physiological and historical aspects of the subject areas under the headings Liver, Biliary, Pancreas, Transplantation,Research, Special Reports, Editorials, Review Articles, Brief Communications and Case Reports. The journal also deals with the basic sciences and experimental work. All submitted papers are reviewed by at least two referees expert in the field of the submit- ted paper. For detailed instructions concerning the submission of manuscripts, please refer to the Instructions tor Authors in each issue ot the journal.展开更多
Hepatobiliary & Pancreatic Diseases International Copyright 2016,Hepatobiliary&Pancreatic Diseases International.All rights reserved.www.hbpdint.comAims and Scope Hepatobiliary&Pancreatic Diseases Internat...Hepatobiliary & Pancreatic Diseases International Copyright 2016,Hepatobiliary&Pancreatic Diseases International.All rights reserved.www.hbpdint.comAims and Scope Hepatobiliary&Pancreatic Diseases International publishes peer-reviewed original papers,reviews(meta-analysis,systematic review)and editorials concerned with clinical practice and research in the fields of hepatobiliary and pancreatic diseases.Papers cover the medical,surgical,展开更多
Aims and Scope Hepatobiliary & Pancreatic Diseases International publishes peer-reviewed original papers, reviews (meta-analysis, systematic review) and editorials concerned with clinical practice and research in ...Aims and Scope Hepatobiliary & Pancreatic Diseases International publishes peer-reviewed original papers, reviews (meta-analysis, systematic review) and editorials concerned with clinical practice and research in the fields of hepatobiliary and pancreatic diseases. Papers cover the medical展开更多
Hepatobiliary & Pancreatic Diseases International(HBPD INT)Copyright 2008,Hepatobiliary & Pancreatic Diseases International.All rights reserved.www.hbpdint.com Aims and Scope Hepatobiliary & Pancreatic Dis...Hepatobiliary & Pancreatic Diseases International(HBPD INT)Copyright 2008,Hepatobiliary & Pancreatic Diseases International.All rights reserved.www.hbpdint.com Aims and Scope Hepatobiliary & Pancreatic Diseases International publishes peer-reviewed original papers,reviews and editorials concerned with clinical practice and research in the展开更多
基金the support from National Natural Science Foundation of China(No.22161132006)Key R&D Program of Zhejiang(No.2024SSYS0036)Westlake University Startup。
文摘Reader proteins that bind specific methyllysine are important to biological functions of lysine methylation,but readers of many methyllysine sites are still unknown.Therefore,development of covalent probes is important to identify readers from cell samples so as to understand biological roles of lysine methylation.Generally,readers bind methyllysine via aromatic cages that contain tryptophan,tyrosine and phenylalanine,that offer a unique motif for selective crosslinking.We recently reported a site-selective tryptophan crosslinking strategy based on dimethylsulfonium that mimics dimethyllysine to crosslink tryptophan in aromatic cages of readers.Since tyrosine is a key residue for binding affinity to methyllysine,especially some readers that do not contain tryptophan residues in the binding pocket.Here we developed strategies of site-selective crosslinking to tyrosine.Ultraviolet(UV)source was applied to excite tyrosine at neutral pH or phenoxide at basic p H,and subsequent single-electron transfer(SET)from Tyr*to sulfonium inside the binding pocket enables selective crosslinking.In consequence,methyllysine readers with tyrosine-containing aromatic cages could be selectively crosslinked by site-specific sulfonium peptide probes.In addition,we expanded substrates from aromatic cages to tyrosine residues of proximate contact with sulfonium probes.The pair of LgBiT and SmBiT exhibited orthogonal crosslinking in complicated cell samples.As a result,we may expand sulfonium tools to target local tyrosine in future investigations.
文摘In the context of the continuous deepening of the“Double Reduction”policy and the growing demand for quality education,leveled mathematics readers,as an emerging form of publishing that integrates subject education and reading experience,face challenges such as unclear leveling logic,insufficient functional support,and weak user engagement.This paper introduces the 4V marketing theory and constructs an analytical framework from four dimensions:differentiation,functionality,added value,and resonance.Two representative products,“Climbing Mathematics”and“Spark Mathematics,”are selected for a typical case comparison to identify their strengths and weaknesses in content design,service systems,and brand operation,and to extract transferable strategic elements.The study finds that the user-value-oriented strategy based on the 4V model can effectively address the core issues in the market promotion and user relationship building of leveled mathematics readers,providing practical paths and theoretical support for educational publishing institutions to achieve product innovation and brand upgrading in this niche field.
文摘The dissemination of Chinese culture is closely related to the influence of national culture.Chinese culture readers serve as important carriers for cultivating global competence among young people and disseminating Chinese culture.They are also important measures to help different cultures eliminate cultural barriers.However,the current Chinese culture readers are of various kinds,seldom testing the readability and rarely taking consideration of the target readers’accessibility.Therefore,this study explores the factors affecting the readability of different Chinese culture readers from the perspective of English global localization,aiming to explore the ways and methods to enhance the dissemination of Chinese culture.Meanwhile,it conducted a survey on the target readers’competence in language learning and culture awareness.
文摘A quadrature mixer with a shared transconductor stage is analyzed,including voltage conversion gain, linearity, noise figure, and image rejection. The analysis indicates it has better performance than a conventional Gilbert mixer pair in commutating mode. A quadrature down-conversion mixer based on this topology is designed and optimized for an ultra high frequency RFID reader. Operating in the 915MHz ISM band, the presented quadrature mixer measures a conversion gain of 12.5dB,an IIP3 of 10dBm, an IIP2 of 58dBm, and an SSB noise figure of 17.6riB. The chip was fabricated in a 0. 18μm 1P6M RF CMOS process and consumes only 3mA of current from a 1.8V power supply.
基金Supported by NIH Clinical Associate Physician(CAP)Award(Dr.Jutabha),American Society for Gastrointestinal Endoscopy Research Award(Dr.Jutabha),American College of Gastroenterology Capsule Endoscopy Research Award(Dr.Jutabha),NIH General Clinical Research
文摘AIM： To determine the performance of novice readers （4^th year medical students） for detecting capsule endoscopy findings. METHODS： Ten capsule endoscopy cases of small bowel lesions were administered to the readers. Gold standard findings were pre-defined by gastroenterologists. Ten gold standard ＂targets＂ were identified among the 10 cases. Readers were given a 30-min overview of Rapid Reader software and instructed to mark any potential areas of abnormalities. A software program was developed using SAS to analyze the thumbnailed findings. RESULTS： The overall sensitivity for detecting the gold standard findings was 80%. As a group, at least 5 out of 10 readers detected each gold standard finding per recording. All the gold standard targets were identified when the readers＇ results were combined. Incidental finding/false positive rate ranged between 8.2-59.8 per reader. CONCLUSION： A panel of medical students with minimal endoscopic experience can achieve high sensitivity in detecting lesions on capsule endoscopy. A group of novice readers can pre-screen recordings to thumbnail potential areas of small bowel lesions for further review. These thumbnails must be reviewed to determine the clinical relevance. Further studies are ongoing to assess other cohorts.
文摘Reading is a very essential skill in the process of knowledge aqusition,while,effective reading ability is the outcome of the good combination of both time and skills.The differences between good readers and poor reader will be studied in terms of comparisons towards their reading speed,efficiency and reasons behind in the domain of psychological perspectives.
文摘This letter proposes updating the World Journal of Gastroenterology(WJG) with several new items and subsections and seeks the readers' input for these suggested changes. In order to further enhance the scope of the WJG, to make it more interactive, and to accommodate our readers' interest, the editors ask the readers for their input regarding potential new proposed changes and new subsections listed below:(1) new subsection-selected highlights from other related Baishideng Publishing Group world series journals;(2) new subsection-challenging images of the week-a quiz with an answer and references on another page;(3) to re-introduce or restore for some issues the cover page on which selected illustration;(4) new subsection-new hypotheses, new concepts and revisiting old concepts in a short, commentary format;(5) preview of the forthcoming issue articles(title and authors);(6) suggestions submitted by the readers-new topics and initiatives; and(7) additional topic area-molecular basis of gastric/liver diseases as an additional area for articles submitted to the journal.
文摘Aims and Scope World Journal of Emergency Medicine(ISSN 1920-8642,CN 33-1408/R),a quarterly journal,publishes peer-reviewed original research articles,review articles including systematic review and meta-analysis,editorials,commentaries,etc in different fields of emergency medicine.The content of articles could be varied in medical,surgical,radiological,pathological,biochemical,physiological and pharmaceutical aspects of the subject areas.The journal also deals with basic sciences and experimental work.
文摘Copyright 2013, Hepatobiliary & Pancreatic Diseases International. All rights reserved. www.hbpdint.com Aims and Scope Hepatobiliary & Pancreatic Diseases International publishes peer-reviewed original papers, reviews (meta-analysis, systematic review) and editorials concerned with clinical practice and research in the fields of hepatobiliary and pancreatic
文摘Aims and Scope Hepatobiliary & Pancreatic Diseases International publishes peer-reviewed original papers, reviews and editorials concerned with clinical practice and research in the fields of hepatobiliary and pancreatic diseases. Papers cover the medical, surgical, radiological, pathological, biochemical , physiological and historical aspects of the subject areas under the headings Liver, Biliary, Pancreas, Transplantation,Research, Special Reports, Editorials, Review Articles, Brief Communications and Case Reports. The journal also deals with the basic sciences and experimental work. All submitted papers are reviewed by at least two referees expert in the field of the submit- ted paper. For detailed instructions concerning the submission of manuscripts, please refer to the Instructions tor Authors in each issue ot the journal.
文摘Hepatobiliary & Pancreatic Diseases International Copyright 2016,Hepatobiliary&Pancreatic Diseases International.All rights reserved.www.hbpdint.comAims and Scope Hepatobiliary&Pancreatic Diseases International publishes peer-reviewed original papers,reviews(meta-analysis,systematic review)and editorials concerned with clinical practice and research in the fields of hepatobiliary and pancreatic diseases.Papers cover the medical,surgical,
文摘Aims and Scope Hepatobiliary & Pancreatic Diseases International publishes peer-reviewed original papers, reviews (meta-analysis, systematic review) and editorials concerned with clinical practice and research in the fields of hepatobiliary and pancreatic diseases. Papers cover the medical
文摘Hepatobiliary & Pancreatic Diseases International(HBPD INT)Copyright 2008,Hepatobiliary & Pancreatic Diseases International.All rights reserved.www.hbpdint.com Aims and Scope Hepatobiliary & Pancreatic Diseases International publishes peer-reviewed original papers,reviews and editorials concerned with clinical practice and research in the
