Reader proteins that bind specific methyllysine are important to biological functions of lysine methylation,but readers of many methyllysine sites are still unknown.Therefore,development of covalent probes is importan...Reader proteins that bind specific methyllysine are important to biological functions of lysine methylation,but readers of many methyllysine sites are still unknown.Therefore,development of covalent probes is important to identify readers from cell samples so as to understand biological roles of lysine methylation.Generally,readers bind methyllysine via aromatic cages that contain tryptophan,tyrosine and phenylalanine,that offer a unique motif for selective crosslinking.We recently reported a site-selective tryptophan crosslinking strategy based on dimethylsulfonium that mimics dimethyllysine to crosslink tryptophan in aromatic cages of readers.Since tyrosine is a key residue for binding affinity to methyllysine,especially some readers that do not contain tryptophan residues in the binding pocket.Here we developed strategies of site-selective crosslinking to tyrosine.Ultraviolet(UV)source was applied to excite tyrosine at neutral pH or phenoxide at basic p H,and subsequent single-electron transfer(SET)from Tyr*to sulfonium inside the binding pocket enables selective crosslinking.In consequence,methyllysine readers with tyrosine-containing aromatic cages could be selectively crosslinked by site-specific sulfonium peptide probes.In addition,we expanded substrates from aromatic cages to tyrosine residues of proximate contact with sulfonium probes.The pair of LgBiT and SmBiT exhibited orthogonal crosslinking in complicated cell samples.As a result,we may expand sulfonium tools to target local tyrosine in future investigations.展开更多
In the context of the continuous deepening of the“Double Reduction”policy and the growing demand for quality education,leveled mathematics readers,as an emerging form of publishing that integrates subject education ...In the context of the continuous deepening of the“Double Reduction”policy and the growing demand for quality education,leveled mathematics readers,as an emerging form of publishing that integrates subject education and reading experience,face challenges such as unclear leveling logic,insufficient functional support,and weak user engagement.This paper introduces the 4V marketing theory and constructs an analytical framework from four dimensions:differentiation,functionality,added value,and resonance.Two representative products,“Climbing Mathematics”and“Spark Mathematics,”are selected for a typical case comparison to identify their strengths and weaknesses in content design,service systems,and brand operation,and to extract transferable strategic elements.The study finds that the user-value-oriented strategy based on the 4V model can effectively address the core issues in the market promotion and user relationship building of leveled mathematics readers,providing practical paths and theoretical support for educational publishing institutions to achieve product innovation and brand upgrading in this niche field.展开更多
The thyroid, an endocrine gland located at the base of the neck, produces thyroid hormones (triiodothyronine (T3) and thyroxine (T4)). The production of these hormones is possible by iodine and other nutrients such as...The thyroid, an endocrine gland located at the base of the neck, produces thyroid hormones (triiodothyronine (T3) and thyroxine (T4)). The production of these hormones is possible by iodine and other nutrients such as selenium and certain vitamins. To assess the thyroid disturbances in the mountain population of Benin, a survey was conducted in Natitingou, a mountain town located in the department of Atacora, in the northwest of Benin, on a sample of thirty (30) adults (15 men and 15 women), most of whom are educated. The results of the questionnaire revealed that 43% of the surveyed population acknowledged having knowledge on the mentioned subject and have dietary habits based on the consumption of seafood, and also legumes (20%). The examination of the serum results of the dosage of T3, T4 and TSH hormones revealed cases of thyroid disturbances in the region (36.32% in men and 44.98% in women). The analysis of a comparative table including the “VEDALAB Easy Reader+” and five (05) other readers, presents the performance, reading techniques, principles, advantages and disadvantages of each device. Pending further studies, some recommendations were made at the end of this study to the academic authorities regarding probable cases of dysthyroidism for which additional examinations are required and an awareness for the improvement of dietary habits.展开更多
The dissemination of Chinese culture is closely related to the influence of national culture.Chinese culture readers serve as important carriers for cultivating global competence among young people and disseminating C...The dissemination of Chinese culture is closely related to the influence of national culture.Chinese culture readers serve as important carriers for cultivating global competence among young people and disseminating Chinese culture.They are also important measures to help different cultures eliminate cultural barriers.However,the current Chinese culture readers are of various kinds,seldom testing the readability and rarely taking consideration of the target readers’accessibility.Therefore,this study explores the factors affecting the readability of different Chinese culture readers from the perspective of English global localization,aiming to explore the ways and methods to enhance the dissemination of Chinese culture.Meanwhile,it conducted a survey on the target readers’competence in language learning and culture awareness.展开更多
A quadrature mixer with a shared transconductor stage is analyzed,including voltage conversion gain, linearity, noise figure, and image rejection. The analysis indicates it has better performance than a conventional G...A quadrature mixer with a shared transconductor stage is analyzed,including voltage conversion gain, linearity, noise figure, and image rejection. The analysis indicates it has better performance than a conventional Gilbert mixer pair in commutating mode. A quadrature down-conversion mixer based on this topology is designed and optimized for an ultra high frequency RFID reader. Operating in the 915MHz ISM band, the presented quadrature mixer measures a conversion gain of 12.5dB,an IIP3 of 10dBm, an IIP2 of 58dBm, and an SSB noise figure of 17.6riB. The chip was fabricated in a 0. 18μm 1P6M RF CMOS process and consumes only 3mA of current from a 1.8V power supply.展开更多
A direct conversion receiver with optimized tolerance to local carrier interference is designed and implemented in a 0.18μm 1P6M mixed-signal CMOS process for a 900MHz RFID reader transceiver. A baseband amplifier wi...A direct conversion receiver with optimized tolerance to local carrier interference is designed and implemented in a 0.18μm 1P6M mixed-signal CMOS process for a 900MHz RFID reader transceiver. A baseband amplifier with series feedback topology is proposed to achieve passive mixer buffering,baseband DC cancellation,and signal amplification simultaneously. The receiver has a measured input ldB compression point of - 4dBm and a sensitivity of - 70dBm when 10dB SNR for digital demodulation is required. The receiver is integrated in a reader transceiver chip and consumes 90mA from a 1.8V supply.展开更多
With the massive increase of digital resource based on the network,it becomes very important that how to help readers acquire the information needed according to the individualized requirement and interest in an accur...With the massive increase of digital resource based on the network,it becomes very important that how to help readers acquire the information needed according to the individualized requirement and interest in an accurate,convenient and prompt method. Therefore,it is very urgent to investigate individualized information service of library. In this paper,based on the development of the individualized information service for the reader in the college library,the individualized information service is clarified by using three perspectives,including establishment of individualized information demand model,integration of internal and external resource and protection of the reader privacy. Finally,the corresponding strategy and related measure are presented.展开更多
Among the sundry studies in China on John Donne's The Flea,there has been none done from the perspective of Reader-Oriented Theory to probe into the readers' roles in the construction of meaning.The present ar...Among the sundry studies in China on John Donne's The Flea,there has been none done from the perspective of Reader-Oriented Theory to probe into the readers' roles in the construction of meaning.The present article under Wolfgang Iser's theories,in a further pursuit of the expansion of the gestalt of such studies on the poem,aims at exploring the readers' active roles in complementing,completing and recreating the textual meaning,and differentiating the implied reader and the actual reader through the poem as well as attempting to fulfill the construction of the reading cognitive model through the interpretation of the conceit the flea.展开更多
Many thanks for your continuing trust and support to Chin, ese Nursing Research! For improving the journal＇s quality, we request you to fill out this questionnaire and scan it, then sent it to hlyjywb@vip.163. com, w...Many thanks for your continuing trust and support to Chin, ese Nursing Research! For improving the journal＇s quality, we request you to fill out this questionnaire and scan it, then sent it to hlyjywb@vip.163. com, we will offer you one-year subscription of our journal Chinese Nursing Research. Thanks for your participation!展开更多
Henry James' s first novella Daisy Miller brought him popularity and literary reputation after its publication. Through the application of reader theory and a close reading of the text, this article analyzes Daisy...Henry James' s first novella Daisy Miller brought him popularity and literary reputation after its publication. Through the application of reader theory and a close reading of the text, this article analyzes Daisy's strong personality and her misfortunes during her trip to Europe, and reveals how the novella highlights the conflict between American frankness and innocence and European sophistication. Besides, this article also elaborates on James' unique artistic skills—central consciousness and psychological analysis through his choice of narrative point of view, which contribute to the theme and the artistic charm of DaisyMiller.展开更多
基金the support from National Natural Science Foundation of China(No.22161132006)Key R&D Program of Zhejiang(No.2024SSYS0036)Westlake University Startup。
文摘Reader proteins that bind specific methyllysine are important to biological functions of lysine methylation,but readers of many methyllysine sites are still unknown.Therefore,development of covalent probes is important to identify readers from cell samples so as to understand biological roles of lysine methylation.Generally,readers bind methyllysine via aromatic cages that contain tryptophan,tyrosine and phenylalanine,that offer a unique motif for selective crosslinking.We recently reported a site-selective tryptophan crosslinking strategy based on dimethylsulfonium that mimics dimethyllysine to crosslink tryptophan in aromatic cages of readers.Since tyrosine is a key residue for binding affinity to methyllysine,especially some readers that do not contain tryptophan residues in the binding pocket.Here we developed strategies of site-selective crosslinking to tyrosine.Ultraviolet(UV)source was applied to excite tyrosine at neutral pH or phenoxide at basic p H,and subsequent single-electron transfer(SET)from Tyr*to sulfonium inside the binding pocket enables selective crosslinking.In consequence,methyllysine readers with tyrosine-containing aromatic cages could be selectively crosslinked by site-specific sulfonium peptide probes.In addition,we expanded substrates from aromatic cages to tyrosine residues of proximate contact with sulfonium probes.The pair of LgBiT and SmBiT exhibited orthogonal crosslinking in complicated cell samples.As a result,we may expand sulfonium tools to target local tyrosine in future investigations.
文摘In the context of the continuous deepening of the“Double Reduction”policy and the growing demand for quality education,leveled mathematics readers,as an emerging form of publishing that integrates subject education and reading experience,face challenges such as unclear leveling logic,insufficient functional support,and weak user engagement.This paper introduces the 4V marketing theory and constructs an analytical framework from four dimensions:differentiation,functionality,added value,and resonance.Two representative products,“Climbing Mathematics”and“Spark Mathematics,”are selected for a typical case comparison to identify their strengths and weaknesses in content design,service systems,and brand operation,and to extract transferable strategic elements.The study finds that the user-value-oriented strategy based on the 4V model can effectively address the core issues in the market promotion and user relationship building of leveled mathematics readers,providing practical paths and theoretical support for educational publishing institutions to achieve product innovation and brand upgrading in this niche field.
文摘The thyroid, an endocrine gland located at the base of the neck, produces thyroid hormones (triiodothyronine (T3) and thyroxine (T4)). The production of these hormones is possible by iodine and other nutrients such as selenium and certain vitamins. To assess the thyroid disturbances in the mountain population of Benin, a survey was conducted in Natitingou, a mountain town located in the department of Atacora, in the northwest of Benin, on a sample of thirty (30) adults (15 men and 15 women), most of whom are educated. The results of the questionnaire revealed that 43% of the surveyed population acknowledged having knowledge on the mentioned subject and have dietary habits based on the consumption of seafood, and also legumes (20%). The examination of the serum results of the dosage of T3, T4 and TSH hormones revealed cases of thyroid disturbances in the region (36.32% in men and 44.98% in women). The analysis of a comparative table including the “VEDALAB Easy Reader+” and five (05) other readers, presents the performance, reading techniques, principles, advantages and disadvantages of each device. Pending further studies, some recommendations were made at the end of this study to the academic authorities regarding probable cases of dysthyroidism for which additional examinations are required and an awareness for the improvement of dietary habits.
文摘The dissemination of Chinese culture is closely related to the influence of national culture.Chinese culture readers serve as important carriers for cultivating global competence among young people and disseminating Chinese culture.They are also important measures to help different cultures eliminate cultural barriers.However,the current Chinese culture readers are of various kinds,seldom testing the readability and rarely taking consideration of the target readers’accessibility.Therefore,this study explores the factors affecting the readability of different Chinese culture readers from the perspective of English global localization,aiming to explore the ways and methods to enhance the dissemination of Chinese culture.Meanwhile,it conducted a survey on the target readers’competence in language learning and culture awareness.
文摘A quadrature mixer with a shared transconductor stage is analyzed,including voltage conversion gain, linearity, noise figure, and image rejection. The analysis indicates it has better performance than a conventional Gilbert mixer pair in commutating mode. A quadrature down-conversion mixer based on this topology is designed and optimized for an ultra high frequency RFID reader. Operating in the 915MHz ISM band, the presented quadrature mixer measures a conversion gain of 12.5dB,an IIP3 of 10dBm, an IIP2 of 58dBm, and an SSB noise figure of 17.6riB. The chip was fabricated in a 0. 18μm 1P6M RF CMOS process and consumes only 3mA of current from a 1.8V power supply.
基金the Science and Technology Commission of Shanghai Municipality(No.057062010)the EU BRIDGE Project(No.033546)~~
文摘A direct conversion receiver with optimized tolerance to local carrier interference is designed and implemented in a 0.18μm 1P6M mixed-signal CMOS process for a 900MHz RFID reader transceiver. A baseband amplifier with series feedback topology is proposed to achieve passive mixer buffering,baseband DC cancellation,and signal amplification simultaneously. The receiver has a measured input ldB compression point of - 4dBm and a sensitivity of - 70dBm when 10dB SNR for digital demodulation is required. The receiver is integrated in a reader transceiver chip and consumes 90mA from a 1.8V supply.
文摘With the massive increase of digital resource based on the network,it becomes very important that how to help readers acquire the information needed according to the individualized requirement and interest in an accurate,convenient and prompt method. Therefore,it is very urgent to investigate individualized information service of library. In this paper,based on the development of the individualized information service for the reader in the college library,the individualized information service is clarified by using three perspectives,including establishment of individualized information demand model,integration of internal and external resource and protection of the reader privacy. Finally,the corresponding strategy and related measure are presented.
文摘Among the sundry studies in China on John Donne's The Flea,there has been none done from the perspective of Reader-Oriented Theory to probe into the readers' roles in the construction of meaning.The present article under Wolfgang Iser's theories,in a further pursuit of the expansion of the gestalt of such studies on the poem,aims at exploring the readers' active roles in complementing,completing and recreating the textual meaning,and differentiating the implied reader and the actual reader through the poem as well as attempting to fulfill the construction of the reading cognitive model through the interpretation of the conceit the flea.
文摘Many thanks for your continuing trust and support to Chin, ese Nursing Research! For improving the journal＇s quality, we request you to fill out this questionnaire and scan it, then sent it to hlyjywb@vip.163. com, we will offer you one-year subscription of our journal Chinese Nursing Research. Thanks for your participation!
文摘Henry James' s first novella Daisy Miller brought him popularity and literary reputation after its publication. Through the application of reader theory and a close reading of the text, this article analyzes Daisy's strong personality and her misfortunes during her trip to Europe, and reveals how the novella highlights the conflict between American frankness and innocence and European sophistication. Besides, this article also elaborates on James' unique artistic skills—central consciousness and psychological analysis through his choice of narrative point of view, which contribute to the theme and the artistic charm of DaisyMiller.
