RNA结合基序蛋白38(RNA binding motif protein 38,RBM38)是一种RNA结合蛋白,在正常细胞中通过调控RNA参与基因转录后调控,从而影响细胞增殖、细胞周期循环、肌源性分化等多种生物学过程。目前已知RBM38可与p53、mdm2、p63、p73、p21、c...RNA结合基序蛋白38(RNA binding motif protein 38,RBM38)是一种RNA结合蛋白,在正常细胞中通过调控RNA参与基因转录后调控,从而影响细胞增殖、细胞周期循环、肌源性分化等多种生物学过程。目前已知RBM38可与p53、mdm2、p63、p73、p21、c-Myc、MIC-1等多个目的基因的3′非翻译区(3′UTR)结合,调节相应mRNA的稳定性,从而在肿瘤进展中发挥关键作用。在多种恶性肿瘤中也已发现RBM38异常表达,但在不同肿瘤中表达存在差异,并且发挥着抑癌和促癌双重作用。本文对RBM38与恶性肿瘤的关系作一综述。展开更多
RNA-binding motif protein 38(Rbm38),also known as RNPC1,is a major regulator of post-transcriptional gene expression.It represents a potential candidate gene linked to the susceptibility of type 2 diabetes,and decreas...RNA-binding motif protein 38(Rbm38),also known as RNPC1,is a major regulator of post-transcriptional gene expression.It represents a potential candidate gene linked to the susceptibility of type 2 diabetes,and decreased RBM38 expression can enhance the proliferation of pancreatic cancer cells in humans.However,its role in pancreatic development remains elusive.In this study,we explored the function of Rbm38 using zebrafish as a model.Pancreatic expression of Rbm38 is present at larval stages and is controlled by several transcription factors acting on specific rbm38 promoter regions.The loss of Rbm38 leads to abnormal pancreatic enlargement.Mechanistically,Rbm38 is involved in several aspects of post-transcriptional regulation of pancreatic gene expression.It destabilizes pdx1 transcripts by binding to the 3′-untranslated region and regulates alternative splicing of key pancreatic transcription factor genes,including isl1a,smad2,and nkx2.2a.These findings elucidate the role of Rbm38 in pancreatic development and highlight its significance in maintaining pancreatic homeostasis.展开更多
目的:分析肺腺癌组织中RNA结合基序蛋白38基因(RNA-binding motif protein 38,RBM38)及抑癌基因p53 m RNA和蛋白的表达情况,探讨其在肺腺癌发生发展中的意义。方法:取2012年10月至2015年6月新疆医科大学附属肿瘤医院收治的50例肺腺癌患...目的:分析肺腺癌组织中RNA结合基序蛋白38基因(RNA-binding motif protein 38,RBM38)及抑癌基因p53 m RNA和蛋白的表达情况,探讨其在肺腺癌发生发展中的意义。方法:取2012年10月至2015年6月新疆医科大学附属肿瘤医院收治的50例肺腺癌患者的肿瘤组织标本作为实验组,相对应的癌旁组织标本作为对照组。用RT-PCR法检测两组中RBM38及p53m RNA相对表达量,用Western blotting法检测两组中RBM38及p53蛋白的相对表达量。结果:实验组RBM38 m RNA及蛋白相对表达量(0.357±0.170、0.294±0.149)均高于对照组(0.271±0.128、0.206±0.099),实验组p53 m RNA及蛋白(0.457±0.208、0.671±0.200)相对表达量均高于对照组(0.308±0.167、0.332±0.071),差异均有统计学意义(均P<0.01)。RBM38表达与肺腺癌患者TNM分期、浸润深度有关(P<0.05),p53表达与患者TNM分期有关(P<0.05)。实验组RBM38与p53蛋白表达呈负相关(r=-0.626,P<0.01)。结论:肺腺癌组织RBM38、p53 m RNA及蛋白表达均高于癌旁组织,两者均与患者TNM分期等病理参数关系密切。随着RBM38蛋白表达的增加p53蛋白随之减少,RBM38可能通过抑制p53的翻译从而促进肺癌的发生发展,RBM38可能成为肺腺癌分子靶向治疗的靶点。展开更多
文摘RNA结合基序蛋白38(RNA binding motif protein 38,RBM38)是一种RNA结合蛋白,在正常细胞中通过调控RNA参与基因转录后调控,从而影响细胞增殖、细胞周期循环、肌源性分化等多种生物学过程。目前已知RBM38可与p53、mdm2、p63、p73、p21、c-Myc、MIC-1等多个目的基因的3′非翻译区(3′UTR)结合,调节相应mRNA的稳定性,从而在肿瘤进展中发挥关键作用。在多种恶性肿瘤中也已发现RBM38异常表达,但在不同肿瘤中表达存在差异,并且发挥着抑癌和促癌双重作用。本文对RBM38与恶性肿瘤的关系作一综述。
基金supported by grants from the Science&Technology Innovation Project of Laoshan Laboratory(LSKJ202203204)the National Natural Science Foundation of China(NSFC)(32471162,32171139,and 31872187)Qingdao Postdoctoral Science Foundation(QDBSH20240102157).
文摘RNA-binding motif protein 38(Rbm38),also known as RNPC1,is a major regulator of post-transcriptional gene expression.It represents a potential candidate gene linked to the susceptibility of type 2 diabetes,and decreased RBM38 expression can enhance the proliferation of pancreatic cancer cells in humans.However,its role in pancreatic development remains elusive.In this study,we explored the function of Rbm38 using zebrafish as a model.Pancreatic expression of Rbm38 is present at larval stages and is controlled by several transcription factors acting on specific rbm38 promoter regions.The loss of Rbm38 leads to abnormal pancreatic enlargement.Mechanistically,Rbm38 is involved in several aspects of post-transcriptional regulation of pancreatic gene expression.It destabilizes pdx1 transcripts by binding to the 3′-untranslated region and regulates alternative splicing of key pancreatic transcription factor genes,including isl1a,smad2,and nkx2.2a.These findings elucidate the role of Rbm38 in pancreatic development and highlight its significance in maintaining pancreatic homeostasis.
文摘目的:分析肺腺癌组织中RNA结合基序蛋白38基因(RNA-binding motif protein 38,RBM38)及抑癌基因p53 m RNA和蛋白的表达情况,探讨其在肺腺癌发生发展中的意义。方法:取2012年10月至2015年6月新疆医科大学附属肿瘤医院收治的50例肺腺癌患者的肿瘤组织标本作为实验组,相对应的癌旁组织标本作为对照组。用RT-PCR法检测两组中RBM38及p53m RNA相对表达量,用Western blotting法检测两组中RBM38及p53蛋白的相对表达量。结果:实验组RBM38 m RNA及蛋白相对表达量(0.357±0.170、0.294±0.149)均高于对照组(0.271±0.128、0.206±0.099),实验组p53 m RNA及蛋白(0.457±0.208、0.671±0.200)相对表达量均高于对照组(0.308±0.167、0.332±0.071),差异均有统计学意义(均P<0.01)。RBM38表达与肺腺癌患者TNM分期、浸润深度有关(P<0.05),p53表达与患者TNM分期有关(P<0.05)。实验组RBM38与p53蛋白表达呈负相关(r=-0.626,P<0.01)。结论:肺腺癌组织RBM38、p53 m RNA及蛋白表达均高于癌旁组织,两者均与患者TNM分期等病理参数关系密切。随着RBM38蛋白表达的增加p53蛋白随之减少,RBM38可能通过抑制p53的翻译从而促进肺癌的发生发展,RBM38可能成为肺腺癌分子靶向治疗的靶点。
