Ginsenoside Rb3(GRb3)is an active ingredient extracted from Panax ginseng,which is known to enhance blood supply to heart and treat a variety of cardiac diseases.The aim of this study was to investigate the effect of ...Ginsenoside Rb3(GRb3)is an active ingredient extracted from Panax ginseng,which is known to enhance blood supply to heart and treat a variety of cardiac diseases.The aim of this study was to investigate the effect of GRb3 on myocardial injury induced by sleep deprivation and its mechanisms.GRb320 mg/kg group showed lower heart rate((438.20±10.06)bpm),creatine kinase((27.43±2.85)U/mg pro)and lactate dehydrogenase((412.90±35.65)U/L)than SD group((592.00±7.78)bpm,(44.18±1.24)U/mg pro,(526.60±38.18)U/L).GRb3 improved myocardial tissue damage and abnormal mitochondrial morphological changes.GRb3 also reduced the abnormal proteins expression of atrial natriuretic peptide(ANP),sirtuin 3(SIRT3),tumor protein P53(P53),solute carrier family 7 member 11(SLC7A11),glutathione peroxidase 4(GPX4),transferrin receptor(TFRC),acyl-CoA synthetase long chain family member 4(ACSL4),heme oxygenase-1(HO-1),ferritin heavy chain 1(FTH1),ferroptosis suppressor protein 1(FSP1).GRb3 ameliorated oxidative stress,such as malondialdehyde(MDA),glutathione(GSH),and superoxide dismutase(SOD).GRb3 also decreased reactive oxygen species(ROS)content,restored cellular abnormal mitochondrial morphology and reversed the expression of ferroptosis-related proteins on H9c2 cells pretreated with Erastin.While the above effect could be reversed by 3-(1H-1,2,3-triazol-4-yl)pyridine(3-TYP).In summary,GRb3 exerts cardioprotective effects by regulating SIRT3/P53/SLC7A11/GPX4 axis to inhibit myocardial ferroptosis in sleep deprived mice.The findings imply that GRb3 holds promising potential for innovative approaches to safeguarding human cardiac health.展开更多
人参皂苷Rb3(ginsenoside Rb3,GRb3)是西洋参茎叶总皂苷(Panax quinquefolius Saponin of stem and leaf,PQS)的主要入血成分之一,既往研究显示其具有雌激素受体激动效应。本研究观察GRb3对ox-LDL诱导人脐静脉内皮细胞氧化应激与功能障...人参皂苷Rb3(ginsenoside Rb3,GRb3)是西洋参茎叶总皂苷(Panax quinquefolius Saponin of stem and leaf,PQS)的主要入血成分之一,既往研究显示其具有雌激素受体激动效应。本研究观察GRb3对ox-LDL诱导人脐静脉内皮细胞氧化应激与功能障碍的保护作用。药物干预后检测细胞内SOD、NOS活性与MDA含量,ELISA法进行细胞培养上清液ET-1与NO含量的测定,real time RT-PCR方法检测细胞中eNOS和iNOS的转录水平,Western blotting方法测定丝氨酸/苏氨酸蛋白激酶Akt的磷酸化水平。与ox-LDL损伤模型组相比,GRb3增高细胞内SOD活力,降低细胞内MDA含量与总NOS活性,减少细胞ET-1和NO合成,升高eNOS的转录水平及降低iNOS的转录水平,且能调节Akt的磷酸化水平,从而发挥保护作用,给予雌激素受体抑制剂ICI182780预处理后上述保护作用消失。结果提示,GRb3可通过雌激素受体的介导发挥抗ox-LDL对内皮细胞的损伤作用,其作用效应与17β-雌二醇相似。展开更多
基金financially supported by the Opening Project of Shanghai Key Laboratory of Compound Chinese Medicines(21DZ2270500)Organizational Key Research and Development Program of Shanghai University of Traditional Chinese Medicine(2023YZZ02).
文摘Ginsenoside Rb3(GRb3)is an active ingredient extracted from Panax ginseng,which is known to enhance blood supply to heart and treat a variety of cardiac diseases.The aim of this study was to investigate the effect of GRb3 on myocardial injury induced by sleep deprivation and its mechanisms.GRb320 mg/kg group showed lower heart rate((438.20±10.06)bpm),creatine kinase((27.43±2.85)U/mg pro)and lactate dehydrogenase((412.90±35.65)U/L)than SD group((592.00±7.78)bpm,(44.18±1.24)U/mg pro,(526.60±38.18)U/L).GRb3 improved myocardial tissue damage and abnormal mitochondrial morphological changes.GRb3 also reduced the abnormal proteins expression of atrial natriuretic peptide(ANP),sirtuin 3(SIRT3),tumor protein P53(P53),solute carrier family 7 member 11(SLC7A11),glutathione peroxidase 4(GPX4),transferrin receptor(TFRC),acyl-CoA synthetase long chain family member 4(ACSL4),heme oxygenase-1(HO-1),ferritin heavy chain 1(FTH1),ferroptosis suppressor protein 1(FSP1).GRb3 ameliorated oxidative stress,such as malondialdehyde(MDA),glutathione(GSH),and superoxide dismutase(SOD).GRb3 also decreased reactive oxygen species(ROS)content,restored cellular abnormal mitochondrial morphology and reversed the expression of ferroptosis-related proteins on H9c2 cells pretreated with Erastin.While the above effect could be reversed by 3-(1H-1,2,3-triazol-4-yl)pyridine(3-TYP).In summary,GRb3 exerts cardioprotective effects by regulating SIRT3/P53/SLC7A11/GPX4 axis to inhibit myocardial ferroptosis in sleep deprived mice.The findings imply that GRb3 holds promising potential for innovative approaches to safeguarding human cardiac health.
文摘人参皂苷Rb3(ginsenoside Rb3,GRb3)是西洋参茎叶总皂苷(Panax quinquefolius Saponin of stem and leaf,PQS)的主要入血成分之一,既往研究显示其具有雌激素受体激动效应。本研究观察GRb3对ox-LDL诱导人脐静脉内皮细胞氧化应激与功能障碍的保护作用。药物干预后检测细胞内SOD、NOS活性与MDA含量,ELISA法进行细胞培养上清液ET-1与NO含量的测定,real time RT-PCR方法检测细胞中eNOS和iNOS的转录水平,Western blotting方法测定丝氨酸/苏氨酸蛋白激酶Akt的磷酸化水平。与ox-LDL损伤模型组相比,GRb3增高细胞内SOD活力,降低细胞内MDA含量与总NOS活性,减少细胞ET-1和NO合成,升高eNOS的转录水平及降低iNOS的转录水平,且能调节Akt的磷酸化水平,从而发挥保护作用,给予雌激素受体抑制剂ICI182780预处理后上述保护作用消失。结果提示,GRb3可通过雌激素受体的介导发挥抗ox-LDL对内皮细胞的损伤作用,其作用效应与17β-雌二醇相似。
