Rapid diagnosis of Salmonella is crucial for the effective control of food safety incidents, especially in regions with poor hygiene conditions. Polymerase chain reaction(PCR), as a promising tool for Salmonella detec...Rapid diagnosis of Salmonella is crucial for the effective control of food safety incidents, especially in regions with poor hygiene conditions. Polymerase chain reaction(PCR), as a promising tool for Salmonella detection, is facing a lack of simple and fast sensing methods that are compatible with field applications in resource-limited areas. In this work, we developed a sensing approach to identify PCR-amplified Salmonella genomic DNA with the naked eye in a snapshot. Based on the ratiometric fiuorescence signals from SYBR Green Ⅰ and Hydroxyl naphthol blue, positive samples stood out from negative ones with a distinct color pattern under UV exposure. The proposed sensing scheme enabled highly specific identification of Salmonella with a detection limit at the single-copy level. Also, as a supplement to the intuitive naked-eye visualization results, numerical analysis of the colored images was available with a smartphone app to extract RGB values from colored images. This work provides a simple, rapid, and user-friendly solution for PCR identification, which promises great potential in molecular diagnosis of Salmonella and other pathogens in field.展开更多
Manganese(Mn),an essential trace element in the human body,plays critical roles in many biological processes.Recent studies have discovered that Mn^(2+)may promote or directly activate the cGAS-STING pathway,thereby s...Manganese(Mn),an essential trace element in the human body,plays critical roles in many biological processes.Recent studies have discovered that Mn^(2+)may promote or directly activate the cGAS-STING pathway,thereby subsequently initiating the natural immune response and augmenting antitumor therapy.However,the current lack of accurate methods for Mn^(2+)determination in cells significantly limits their mechanism investigation;hence,it is urgent to establish novel tools to detect Mn^(2+)in cells.In this study,the dual-emission carbon dots were initially synthesized via the one-pot hydrothermal method employing L-aspartic acid and p-phenylenediamine as raw materials.In the presence of Mn^(2+),the emission peak centered at 350 nm exhibited significant enhancement,whereas another peak at 610 nm remained stable.Consequently,a ratiometric sensor for Mn^(2+)determination was established using the signal at 350 nm as the responsive signal and the signal at 610 nm as an internal reference.Under the optimal condition,a good linear relationship was achieved between the F350/F610 value and Mn^(2+)concentration ranging from 0.9 to 15μmol/L,with a calculated LOD of 61 nmol/L.Benefiting from the special Mn^(2+)-induced ratiometric approach,this method demonstrates outstanding sensitivity,selectivity,and stability,rendering it applicable for Mn^(2+)determination in complex biological samples,as well as Mn^(2+)imaging in MKN-45 and LO2 cells.展开更多
Calcium ions(Ca^(2+))and manganese ions(Mn^(2+))are essential for sustaining life activities and are key monitoring indicators in drinking water.Developing highly sensitive,selective,and portable detection methods for...Calcium ions(Ca^(2+))and manganese ions(Mn^(2+))are essential for sustaining life activities and are key monitoring indicators in drinking water.Developing highly sensitive,selective,and portable detection methods for Ca^(2+)and Mn^(2+)is significant for water quality monitoring and human health.In this paper,blue fluorescent Ti3C2 MXene-based quantum dots(MQDs,λ_(em)=445 nm)are prepared using Ti_(3)C_(2)MXene as the precursor.Through the chelation effect of ethylene diamine tetraacetic acid(EDTA),a blue and red dual-emission fluorescent probe,MQDs-EDTA-Eu^(3+)-DPA,was constructed.Herein,dipicolinic acid(DPA)acts as an absorbing ligand and significantly enhances the red fluorescence of europium ions(Eu^(3+))at 616 nm through the“antenna effect”.The blue fluorescence of MQDs serves as an internal reference signal.High concentrations of Ca^(2+)can quench the red fluorescence of Eu^(3+)-DPA;Mn^(2+)can be excited to emit purple fluorescence at 380 nm after coordinating with DPA,red fluorescence of Eu^(3+)-DPA serves as the internal reference signal.Based on the above two fluorescence intensity changes,ratiometric fluorescence detection methods for Ca^(2+)and Mn^(2+)are established.The fluorescence intensity ratio(IF_(616)/IF_(445))exhibits a linear relationship with Ca^(2+)in the range of 35-120μmol/L,with a detection limit of 5.98μmol/L.The fluorescence intensity ratio(IF_(380)/IF_(616))shows good linearity with Mn^(2+)in the range of 0-14μmol/L,with a detection limit of 28.6 nmol/L.This method was successfully applied to the quantitative analysis of Ca^(2+)and Mn^(2+)in commercially available mineral water(Nongfu Spring,Ganten,and Evergrande),with recovery rates of 80.6%-117%and relative standard deviations(RSD)of 0.76%-4.6%.Additionally,by preparing MQD-based fluorescent test strips,visual detections of Ca^(2+)and Mn^(2+)are achieved.This work demonstrates the application potential of MQDs in the field of visual fluorescence sensing of ions in water quality.展开更多
Photodynamic therapy(PDT)has emerged as a promising approach for tumor treatment due to its noninvasiveness and high selectivity.However,the off-target activation of phototoxicity and the limited availability of tumor...Photodynamic therapy(PDT)has emerged as a promising approach for tumor treatment due to its noninvasiveness and high selectivity.However,the off-target activation of phototoxicity and the limited availability of tumor-specific biomarkers pose challenges for effective PDT.Here,we present the development of a novel ratiometric near-infrared-II(NIR-II)fluorescent organic nanoprobe,BTz-IC@IR1061,which responds specifically to hypochlorite(HClO)within tumors.This nanoprobe allows ratiometric fluorescence imaging to monitor and guide activated tumor PDT.BTz-IC@IR1061 nanoparticles were synthesized by codoping the small molecule dye BTz-IC,which generates reactive oxygen species(ROS),with the commercial dye IR1061.The presence of HClO selectively activates the fluorescence and photodynamic properties of BTz-IC while destroying IR1061,enabling controlled release of ROS for tumor therapy.We demonstrated the high selectivity of the nanoprobe for HClO,as well as its excellent photostability,photoacoustic imaging capability,and photothermal effects.Furthermore,in vivo studies revealed effective tumor targeting and remarkable tumor growth inhibition through tumor-activated PDT.Our findings highlight the potential of BTz-IC@IR1061 as a promising tool for tumor-specific PDT,providing new opportunities for precise and controlled cancer therapy.展开更多
Cancer is a serious global health issue,and exploring effective treatment methods is of great significance for cancer prevention and control.Carbon monoxide(CO),as an important gas signaling molecule in the life syste...Cancer is a serious global health issue,and exploring effective treatment methods is of great significance for cancer prevention and control.Carbon monoxide(CO),as an important gas signaling molecule in the life system,has been proven to have good anti-cancer effects.However,how to controllably,safely,and effectively deliver CO to the tumor site for clinical treatment remains a challenge.Herein,a new metal-free CO-releasing molecule COR-XAC was developed for controlling CO release and cancer therapy.COR-XAC is based on the hybrid of 3-hydroxyl flavone and oxanthracene fluorophores,showing visible light-controlled CO-releasing properties and near-infrared(NIR)ratiometric fluorescence changes at 690 and 760 nm.COR-XAC shows low cytotoxicity and can be successfully applied to release CO in cells and tumors,and the CO-releasing and delivery process could be monitored by its own NIR ratiometric fluorescence changes.More importantly,the anti-cancer performance of COR-XAC was evaluated in 4T1 tumor mice,and it was found that COR-XAC plus light illumination showed excellent tumor inhibition effect,which provided a promising new effective method for cancer treatment.展开更多
Mitochondria are crucial organelles responsible for maintaining cell growth,and their homeostasis is closely linked to p H regulation.Physiologically,mitochondria exhibit a weakly alkaline state(pH~8.0).However,when s...Mitochondria are crucial organelles responsible for maintaining cell growth,and their homeostasis is closely linked to p H regulation.Physiologically,mitochondria exhibit a weakly alkaline state(pH~8.0).However,when subjected to stress stimuli that cause damage,cells initiate the process of mitophagy,resulting in mitochondrial acidification.Therefore,monitoring changes in mitochondrial p H to comprehend the physiological processes associated with mitophagy is essential.In this study,we developed an asymmetric pentamethine cyanine dye Cy5.5-H-Cy N as a probe for continuous monitoring of mitophagy in living cells.By incorporating an azaindole structure into the dye molecule,a ratiometric fluorescence response was achieved that is specifically responsive to p H variations while preserving its ability to target mitochondria and emit near-infrared fluorescence.Through various methods inducing mitophagy,Cy5.5-H-Cy N was employed to determine mitochondrial p H quantitatively,demonstrating its suitability as an ideal probe for continuous monitoring of mitophagy in living cells.展开更多
The fabrication of bioreceptor-free method for accurate and sensitive detection of ochratoxin A(OTA) in cereal is critical, but still a significant challenge to mitigate risks to food industries and public health. In ...The fabrication of bioreceptor-free method for accurate and sensitive detection of ochratoxin A(OTA) in cereal is critical, but still a significant challenge to mitigate risks to food industries and public health. In this study, a smartphone-ratiometric fiuorescence sensor for the ultrasensitive detection of OTA is developed based on a porphyrinic metal-organic framework and silica nanoparticle composite(Zr-MOF/Si NPs)away from the use of antibodies and aptamers. Due to the excellent recognition ability of Zr-MOF and good storage stability of Si NPs, OTA is detected by Zr-MOF/Si NPs with a wide linear range of 0.05–1000 ng/m L and low detection limit of 0.016 ng/m L. Moreover, the red–blue ratio values of the fiuorescence images are extracted through the smartphone color recognizer application with a limit of detection of 1.74 ng/m L, lower than the permissible content of OTA in cereal prescribed by World Health Organization. This sensing platform has been successfully applied in maize samples with superior repeatability and satisfactory recoveries, providing a novel way for simple and label-free analysis of OTA in cereal.展开更多
Developing an accurate and visual sensing strategy for trace levels of fluoroquinolone residues that pose threat to food safety and human health is highly desired but remains challenging.Herein,a target selfcalibratio...Developing an accurate and visual sensing strategy for trace levels of fluoroquinolone residues that pose threat to food safety and human health is highly desired but remains challenging.Herein,a target selfcalibration ratiometric fluorescent sensing platform has been designed for sensitive visual detection of levofloxacin(LEV)based on fluorescent europium metal-organic framework(Eu-MOF)probe.Specifically,the Eu-MOF was facilely synthesized via directly mixing Eu^(3+)with 1,10-phenanthroline-2,9-dicarboxylic acid(PDA)ligand at room temperature,which exhibited well-stable red fluorescence at 612 nm.Upon the addition of target LEV,the significant fluorescence quenching from Eu^(3+)was observed owing to the inner filter effect between the Eu-MOF and LEV.While the intrinsic fluorescence for LEV at 462nm was gradually enhanced,thereby realizing the self-calibration ratiometric fluorescence responses to LEV.Through this strategy,LEV can be detected down to 27 nmol/L.Furthermore,a test paper-based Eu-MOF integrated with the smartphone assisted RGB color analysis was exploited for the quantitative monitoring of LEV through the multi-color changes from red to blue,thus achieved portable,convenient and visual detection of LEV in honey and milk samples.Therefore,the developed strategy could provide a useful tool for supporting the practical on-site test in food samples.展开更多
The traditional nanozymes-based ratiometric fluorescence sensing platforms usually necessitate the supplementary addition of fluorescent probes,therefore greatly restricting its convenient and broad application.In thi...The traditional nanozymes-based ratiometric fluorescence sensing platforms usually necessitate the supplementary addition of fluorescent probes,therefore greatly restricting its convenient and broad application.In this study,a highly sensitive and selective ratiometric fluorescence platform for alkaline phosphatase(ALP)detection was established,only employing Prussian blue(PB)nanozymes and a commercially available chromogen of o-phenylenediamine(OPD).PB nanozymes with remarkable peroxidaselike(POD-like)activity can effectively catalyze OPD chromogen to yield 2,3-diaminophenazine(OPDox)with an intense yellow fluorescence at 573 nm emission peak.Target ALP can facilitate ascorbic acid 2-phosphate(AAP)dephosphorylation to generate phosphate and ascorbic acid(AA).Significantly,both these two resultant hydrolysis products could effectively decrease the OPDox generation via a dualpath based inhibition on the PB nanozymes POD-like activity.On the other hand,the generated dehydroascorbic acid(DHAA)from AA oxidation would exclusively react with OPD chromogen to yield3-(dihydroxyethyl)furo[3,4-b]quinoxaline-1-one(DFQ)with a strong blue fluorescent signal at 434nm,which further providing a significant enhancement on the sensing selectivity of ALP detection.As a result,an increased yellow fluorescence of OPDox and decreased blue fluorescence of DFQ could be clearly observed with different ALP addition.A robust linear relationship between the fluorescence ratio of F_(434)/F_(573)and ALP activity ranging from 0.25U/L to 6U/L was obtained,with a low detection limit of 0.112 U/L.This proposed method demonstrates high sensitivity,excellent selectivity,cost-effectiveness,and operational simplicity,yet enabling an effective detection of ALP levels in human serum.展开更多
Gallstones are a common disease worldwide,often leading to obstruction and inflammatory complications,which seriously affect the quality of life of patients.Research has shown that gallstone disease is associated with...Gallstones are a common disease worldwide,often leading to obstruction and inflammatory complications,which seriously affect the quality of life of patients.Research has shown that gallstone disease is associated with ferroptosis,lipid droplets(LDs),and abnormal levels of nitric oxide(NO).Fluorescent probes provide a sensitive and convenient method for detecting important substances in life systems and diseases.However,so far,no fluorescent probes for NO and LDs in gallstone disease have been reported.In this work,an effective ratiometric fluorescent probe LR-NH was designed for the detection of NO in LDs.With an anthracimide fluorophore and a secondary amine as a response site for NO,LR-NH exhibits high selectivity,sensitivity,and attractive ratiometric capability in detecting NO.Importantly,it can target LDs and shows excellent imaging ability for NO in cells and ferroptosis.Moreover,LR-NH can target the gallbladder and image NO in gallstone disease models,providing a unique and unprecedented tool for studying NO in LDs and gallbladder.展开更多
Mixed-lanthanide(Ln) luminescent materials have important application values in ratiometric temperature sensing.Hydrogen-bonded organic frameworks(HOFs) offer a self-supporting network to conveniently tune multiple Ln...Mixed-lanthanide(Ln) luminescent materials have important application values in ratiometric temperature sensing.Hydrogen-bonded organic frameworks(HOFs) offer a self-supporting network to conveniently tune multiple Ln^(3+)ions,but challenges still exist in material design.In this work,we selected two simple organic molecules as hydrogen-bonded building blocks,which are melamine(MA) and 2,5-pyridinedicarboxylic acid(PDC) owning the suitable energy to sensitize Tb^(3+)and Eu^(3+)ions.MA assists PDC molecules to support the network in a new HOF(MA-PDC),where the abundant carboxylic groups enable Ln^(3+)ions to combine with the HOF.Based on the effective energy transfer process,the emission of Tb^(3+)and Eu^(3+)from TbxEu1-x@MA-PDC(x=0.75,0.85,0.90) shows the obvious temperature dependence,which benefits ratiometric temperature detection.Taking Tb0.85Eu0.15@MA-PDC as an example,when temperature varies from 303 to 443 K,the intensity ratio exhibits distinct S-type response based on Mott-Seitz model.It also behaves good relative sensitivity(1.03%/K at 383 K),low temperature resolution(0.059 K) and large reproducibility(>96%).This work demonstrates that functionalization of a simple-component HOF with mixed Ln^(3+)ions is a fantastic strategy to develop novel ratiometric thermometers of both low cost and good performance.展开更多
A 12-metal Zn(Ⅱ)-Nd(Ⅲ) cluster 1(sizes:1.8 nm×2.0 nm×2.0 nm) was synthesized from a long-chain type Schiff base ligand.It displays ratiometric fluorescence response to neopterin(Neo) with high selectivity ...A 12-metal Zn(Ⅱ)-Nd(Ⅲ) cluster 1(sizes:1.8 nm×2.0 nm×2.0 nm) was synthesized from a long-chain type Schiff base ligand.It displays ratiometric fluorescence response to neopterin(Neo) with high selectivity and sensitivity,which can be expressed by the equation I_(545)_(nm)/I_(1060)_(nm)=A·[Neo]^(2)+B·[Neo]+C.1 is used to quantitatively test Neo concentrations in fetal calf serum(FCS) and urine,and the recovery ranges are 98.57%-103.82% and 99.25%-103.50%,respectively,while the relative standard deviations(RSDs) are 7.89%-9.46% and 1.85%-4.16%,respectively.The limits of detection of 1 to Neo in FCS and urine are 0.034 and 0.021 μmol/L,respectively.展开更多
Purely organic room-temperature phosphorescence(RTP)and fluorescence dual-emission materials in aqueous solution have attracted growing attention.Herein,we report a fluorescencephosphorescence dual emission host-vip...Purely organic room-temperature phosphorescence(RTP)and fluorescence dual-emission materials in aqueous solution have attracted growing attention.Herein,we report a fluorescencephosphorescence dual emission host-vip complex by simple assembly of cucurbit[8]uril(CB[8])and4-(4-bromophenyl)pyridinium derivative in water.Macrocyclic confinement and unique 1:2 host-vip structure could effectively inhibit non-radiative transition of the vip and the quenching of water molecule,thus induce effective RTP emission in water(τ_(RTP)=0.472 ms,Φ_(RTP)=1.37%).Specifically,based on competitive binding,this host-vip complex exhibits rapid ratiometric luminescent detection behavior to 3-nitrotyrosine,a specific biomarker of kidney injury,with a low limit of detection of 10.7 nmol/L.This work highlights the great potential of macrocyclic-confinement-derived RTP materials in biomarker detection,and will undoubtedly broaden the utilization scope of RTP.展开更多
Lipid peroxidation(LPO)in foam cells is crucial for regulating atherosclerosis progression.It correlates with lipid uptake and the state of lipid droplets.In this study,we report a lipid droplet-targeted fluorescent L...Lipid peroxidation(LPO)in foam cells is crucial for regulating atherosclerosis progression.It correlates with lipid uptake and the state of lipid droplets.In this study,we report a lipid droplet-targeted fluorescent LPO probe,Ld-LPO.It selectively responds to LPO,resulting in a significant fluorescence shift from 590 to 525 nm,enabling a ratiometric imaging of LPO in lipid droplets.Ld-LPO traces lipid droplets in foam cells,revealing a correlation between LPO and lysosomal engulfment.We found that lipid droplets engulfed by lysosomes exhibit higher LPO,attributed to low-density lipoprotein accumulation in lysosomes.Furthermore,Ld-LPO is compatible with dual-color flow cytometry,facilitating highthroughput analysis of LPO in foam cells.展开更多
Introduction of porosity and fluorescent properties into lanthanide metal-organic frameworks(MOFs)with rational design to achieve multifunctional use is of great significance from the energy and environmental viewpoin...Introduction of porosity and fluorescent properties into lanthanide metal-organic frameworks(MOFs)with rational design to achieve multifunctional use is of great significance from the energy and environmental viewpoint.In this study,a microporous Tb(Ⅲ)-based MOF with the formula of{[Tb2(oba)3(Phen)2](DMF)2(H2O)4}_n(1)was solvothermally prepared via using the mixed ligands of 1,10-phenanthroline(Phen)and 4,4'-oxybis(benzoic acid)(H_(2)oba)as organic connecters.The structural evaluation results indicate that complex 1 is composed of binuclear Tb2(CO_(2))6(Phen)2clusters which are extended by the oba^(2-)ligands to afford a two-fold interpenetrated framework with one-dimensional microporous channels along the b-axis.Gas sorption studies show that the activated 1 demonstrates a high ideal adsorption solution theory(IAST)sorption selectivity of 7.4 toward C_(2)H_(2)in a C_(2)H_(2)/CO_(2)gas mixture,which are further supported by the dynamic breakthrough experiments.The grand canonical Monte Carlo(GCMC)simulation results indicate that the synergistic effects of the H-bond interactions of C_(2)H_(2)with oba^(2-)ligands and the C-H…πinteractions with Phen ligand contribute to the strong binding of the framework toward C_(2)H_(2)molecules.What's more,the luminescent measurements reveal that the emission of 1 features both the characteristic peaks of Phen ligand and Tb(Ⅲ)ion,which could be further applied as a self-calibrating sensor for the Cr(Ⅵ)detection in water.To the best of our knowledge,complex 1 represents the first example of Tb-MOF holding such a high C_(2)H_(2)/CO_(2)selectivity together with ratiometric Cr(Ⅵ)detection performances.展开更多
A ratiometric fluorescence probe,NClO,for the rapid and selective detection of HClO had been designed and synthesized based on a 1,8-naphthalimide derivative.Probe NClO displayed a red emission(λmax=615 nm).In the pr...A ratiometric fluorescence probe,NClO,for the rapid and selective detection of HClO had been designed and synthesized based on a 1,8-naphthalimide derivative.Probe NClO displayed a red emission(λmax=615 nm).In the presence of HClO,the solution of probe NClO gave off a strong green fluorescence(λem,max=520 nm)with a rapid response(within seconds).This probe had been applied to image HClO in living cells and zebra fish.展开更多
Intracellular pH plays a significant role in various biological processes, including cell proliferation,apoptosis, metabolism, enzyme activity and homeostasis. In this work, a novel design strategy for the preparation...Intracellular pH plays a significant role in various biological processes, including cell proliferation,apoptosis, metabolism, enzyme activity and homeostasis. In this work, a novel design strategy for the preparation of pH responsive carbon dots(CDs-pH) for ratiometric intracellular imaging was reported. By using SciFinder database, fluorescent CDs-pH with the required p Kavalue of 6.84 were rationally designed, which is vital important for precise sensing of intracellular pH. As a result, the synthesized CDspH demonstrated robust ability to test pH fluctuations within the physiological range of 5.4-7.4. The CDspH was further utilized for fluorescent ratiometric imaging of pH in living HeLa cells, effectively avoided the influence of autofluorescence from native cellular species. Moreover, real-time monitoring of intracellular pH fluctuation under heat shock was successfully realized. This SciFinder-guided design strategy is simple and flexible, which has a great potential to be used for the development of other types of CDs for various applications.展开更多
The reactive oxygen species(ROS) are tightly associated with endoplasmic reticulum(ER) stress.Thus,the deep and visual insight of aberrant ROS fluctuations in the ER can help us better investigate the ER stress-associ...The reactive oxygen species(ROS) are tightly associated with endoplasmic reticulum(ER) stress.Thus,the deep and visual insight of aberrant ROS fluctuations in the ER can help us better investigate the ER stress-associated pathology.In this work,a fluorescent probe ERC for HOCI detection in the ER based on phenothiazine-derived coumarin platform was developed.In the presence of HOCI,ERC exhibited an emission change from 609 nm to 503 nm within seconds.It also showed high sensitivity(0.44 μmol/L)and superb photostability.Significantly,ERC displayed low cytotoxicity,good cell membrane permeability,and appreciable ER-targetability.Ultimately,the probe was successfully utilized to image exogenous and endogenous HOCl in living cells and reveal the HOCI burst in cisplatin-treated cancer cells.展开更多
Iron is one of the essential trace elements in the human body.It plays an important role in human biology and pathology.Deregulation of iron levels in cells is associated with disease development.In this work,we synth...Iron is one of the essential trace elements in the human body.It plays an important role in human biology and pathology.Deregulation of iron levels in cells is associated with disease development.In this work,we synthesized a novel near-infrared intramolecular charge transfer(ICT)based ratiometric fluorescent probe to detect Fe^(2+),by using naphthalimide and indole moieties as building blocks.Our work showed that the radiometric probe has excellent selectivity,sensitivity and rapid response.Moreover,we could successfully perform real-time monitoring of Fe^(2+) in He La cells and C.elegans.展开更多
We developed a merocyanine-based fluorescent probe,NEPB,for tracing hydrazine(N2 H4)in a ratiometric manner with large Stokes shifts and long emission wavelength.The fluorescence color of probe NEPB changed from green...We developed a merocyanine-based fluorescent probe,NEPB,for tracing hydrazine(N2 H4)in a ratiometric manner with large Stokes shifts and long emission wavelength.The fluorescence color of probe NEPB changed from green to yellow upon addition of hydrazine.Probe NEPB displayed high selectivity and sensitivity to hydrazine in solution,and could ratiometrically monitor N2 H4 in living cells and zebrafish with low cytotoxicity.展开更多
基金supported by the Macao Science and Technology Development Fund(FDCT)(Nos.FDCT 0029/2021/A1,FDCT0002/2021/AKP,004/2023/SKL,0036/2021/APD)University of Macao(No.MYRG-GRG2023-00034-IME,SRG2024-00057IME)+2 种基金Dr.Stanley Ho Medical Development Foundation(No.SHMDF-OIRFS/2024/001)Zhuhai Huafa Group(No.HF-006-2021)Guangdong Science and Technology Department(No.2022A0505030022)。
文摘Rapid diagnosis of Salmonella is crucial for the effective control of food safety incidents, especially in regions with poor hygiene conditions. Polymerase chain reaction(PCR), as a promising tool for Salmonella detection, is facing a lack of simple and fast sensing methods that are compatible with field applications in resource-limited areas. In this work, we developed a sensing approach to identify PCR-amplified Salmonella genomic DNA with the naked eye in a snapshot. Based on the ratiometric fiuorescence signals from SYBR Green Ⅰ and Hydroxyl naphthol blue, positive samples stood out from negative ones with a distinct color pattern under UV exposure. The proposed sensing scheme enabled highly specific identification of Salmonella with a detection limit at the single-copy level. Also, as a supplement to the intuitive naked-eye visualization results, numerical analysis of the colored images was available with a smartphone app to extract RGB values from colored images. This work provides a simple, rapid, and user-friendly solution for PCR identification, which promises great potential in molecular diagnosis of Salmonella and other pathogens in field.
基金Supported by National Natural Science Foundation of China(22264023)Natural Science Foundation of Shaanxi Province(2024JC-YBQN-0150)+2 种基金Yan'an Science and Technology Bureau Project(2023-SFGG-057)Scientific Research Projects of Education Department of Shaanxi Province(22JK0614)PhD Start Fund of Yan'an University(YDBK2022-15)。
文摘Manganese(Mn),an essential trace element in the human body,plays critical roles in many biological processes.Recent studies have discovered that Mn^(2+)may promote or directly activate the cGAS-STING pathway,thereby subsequently initiating the natural immune response and augmenting antitumor therapy.However,the current lack of accurate methods for Mn^(2+)determination in cells significantly limits their mechanism investigation;hence,it is urgent to establish novel tools to detect Mn^(2+)in cells.In this study,the dual-emission carbon dots were initially synthesized via the one-pot hydrothermal method employing L-aspartic acid and p-phenylenediamine as raw materials.In the presence of Mn^(2+),the emission peak centered at 350 nm exhibited significant enhancement,whereas another peak at 610 nm remained stable.Consequently,a ratiometric sensor for Mn^(2+)determination was established using the signal at 350 nm as the responsive signal and the signal at 610 nm as an internal reference.Under the optimal condition,a good linear relationship was achieved between the F350/F610 value and Mn^(2+)concentration ranging from 0.9 to 15μmol/L,with a calculated LOD of 61 nmol/L.Benefiting from the special Mn^(2+)-induced ratiometric approach,this method demonstrates outstanding sensitivity,selectivity,and stability,rendering it applicable for Mn^(2+)determination in complex biological samples,as well as Mn^(2+)imaging in MKN-45 and LO2 cells.
基金The Tertiary Education Scientific Research Project of the Guangzhou Municipal Education Bureau(2024312227)Innovative and Entrepreneurial Projects of Guangzhou University Students(202411078014)+2 种基金Guangzhou University Open Sharing Fund for Instruments and Equipment(2025)National Major Scientific Research Instrument Development Project(22227804)Sub-subject of the National Key Research Project(2023YFB3210100)。
文摘Calcium ions(Ca^(2+))and manganese ions(Mn^(2+))are essential for sustaining life activities and are key monitoring indicators in drinking water.Developing highly sensitive,selective,and portable detection methods for Ca^(2+)and Mn^(2+)is significant for water quality monitoring and human health.In this paper,blue fluorescent Ti3C2 MXene-based quantum dots(MQDs,λ_(em)=445 nm)are prepared using Ti_(3)C_(2)MXene as the precursor.Through the chelation effect of ethylene diamine tetraacetic acid(EDTA),a blue and red dual-emission fluorescent probe,MQDs-EDTA-Eu^(3+)-DPA,was constructed.Herein,dipicolinic acid(DPA)acts as an absorbing ligand and significantly enhances the red fluorescence of europium ions(Eu^(3+))at 616 nm through the“antenna effect”.The blue fluorescence of MQDs serves as an internal reference signal.High concentrations of Ca^(2+)can quench the red fluorescence of Eu^(3+)-DPA;Mn^(2+)can be excited to emit purple fluorescence at 380 nm after coordinating with DPA,red fluorescence of Eu^(3+)-DPA serves as the internal reference signal.Based on the above two fluorescence intensity changes,ratiometric fluorescence detection methods for Ca^(2+)and Mn^(2+)are established.The fluorescence intensity ratio(IF_(616)/IF_(445))exhibits a linear relationship with Ca^(2+)in the range of 35-120μmol/L,with a detection limit of 5.98μmol/L.The fluorescence intensity ratio(IF_(380)/IF_(616))shows good linearity with Mn^(2+)in the range of 0-14μmol/L,with a detection limit of 28.6 nmol/L.This method was successfully applied to the quantitative analysis of Ca^(2+)and Mn^(2+)in commercially available mineral water(Nongfu Spring,Ganten,and Evergrande),with recovery rates of 80.6%-117%and relative standard deviations(RSD)of 0.76%-4.6%.Additionally,by preparing MQD-based fluorescent test strips,visual detections of Ca^(2+)and Mn^(2+)are achieved.This work demonstrates the application potential of MQDs in the field of visual fluorescence sensing of ions in water quality.
基金supported by the National Natural Science Foundation of China(Nos.22374040,U21A20287,21974039,21890744)the Key Projects of National Natural Science Foundation of China(No.22234003)+1 种基金the National Key R&D Program of China(No.2019YFA0210100)the Fundamental Research Funds for the Central Universities.
文摘Photodynamic therapy(PDT)has emerged as a promising approach for tumor treatment due to its noninvasiveness and high selectivity.However,the off-target activation of phototoxicity and the limited availability of tumor-specific biomarkers pose challenges for effective PDT.Here,we present the development of a novel ratiometric near-infrared-II(NIR-II)fluorescent organic nanoprobe,BTz-IC@IR1061,which responds specifically to hypochlorite(HClO)within tumors.This nanoprobe allows ratiometric fluorescence imaging to monitor and guide activated tumor PDT.BTz-IC@IR1061 nanoparticles were synthesized by codoping the small molecule dye BTz-IC,which generates reactive oxygen species(ROS),with the commercial dye IR1061.The presence of HClO selectively activates the fluorescence and photodynamic properties of BTz-IC while destroying IR1061,enabling controlled release of ROS for tumor therapy.We demonstrated the high selectivity of the nanoprobe for HClO,as well as its excellent photostability,photoacoustic imaging capability,and photothermal effects.Furthermore,in vivo studies revealed effective tumor targeting and remarkable tumor growth inhibition through tumor-activated PDT.Our findings highlight the potential of BTz-IC@IR1061 as a promising tool for tumor-specific PDT,providing new opportunities for precise and controlled cancer therapy.
基金supported by the National Natural Science Foundation of China(Nos.22077044 and 21672080)the Natural Science Foundation of Hubei Province(No.2022CFA033)the funding from Wuhan Institute of Photochemistry and Technology(No.GHY2023KF008).
文摘Cancer is a serious global health issue,and exploring effective treatment methods is of great significance for cancer prevention and control.Carbon monoxide(CO),as an important gas signaling molecule in the life system,has been proven to have good anti-cancer effects.However,how to controllably,safely,and effectively deliver CO to the tumor site for clinical treatment remains a challenge.Herein,a new metal-free CO-releasing molecule COR-XAC was developed for controlling CO release and cancer therapy.COR-XAC is based on the hybrid of 3-hydroxyl flavone and oxanthracene fluorophores,showing visible light-controlled CO-releasing properties and near-infrared(NIR)ratiometric fluorescence changes at 690 and 760 nm.COR-XAC shows low cytotoxicity and can be successfully applied to release CO in cells and tumors,and the CO-releasing and delivery process could be monitored by its own NIR ratiometric fluorescence changes.More importantly,the anti-cancer performance of COR-XAC was evaluated in 4T1 tumor mice,and it was found that COR-XAC plus light illumination showed excellent tumor inhibition effect,which provided a promising new effective method for cancer treatment.
基金supported by the Fundamental Research Funds for the Central Universities(Nos.DUT23YG137 and DUT22LAB601)Liaoning Binhai Laboratory(No.LBLB-202303)+1 种基金Liaoning Province Science and Technology Joint Fund(Nos.2023JH2/101800039 and 2023JH2/101800037)National Natural Science Foundation of China(Nos.21925802,22090011,and 21878039)。
文摘Mitochondria are crucial organelles responsible for maintaining cell growth,and their homeostasis is closely linked to p H regulation.Physiologically,mitochondria exhibit a weakly alkaline state(pH~8.0).However,when subjected to stress stimuli that cause damage,cells initiate the process of mitophagy,resulting in mitochondrial acidification.Therefore,monitoring changes in mitochondrial p H to comprehend the physiological processes associated with mitophagy is essential.In this study,we developed an asymmetric pentamethine cyanine dye Cy5.5-H-Cy N as a probe for continuous monitoring of mitophagy in living cells.By incorporating an azaindole structure into the dye molecule,a ratiometric fluorescence response was achieved that is specifically responsive to p H variations while preserving its ability to target mitochondria and emit near-infrared fluorescence.Through various methods inducing mitophagy,Cy5.5-H-Cy N was employed to determine mitochondrial p H quantitatively,demonstrating its suitability as an ideal probe for continuous monitoring of mitophagy in living cells.
基金funding from the National Natural Science Foundation of China (No. 31871877)the National Key Research and Development Program of China (No. 2019YFC1606303)。
文摘The fabrication of bioreceptor-free method for accurate and sensitive detection of ochratoxin A(OTA) in cereal is critical, but still a significant challenge to mitigate risks to food industries and public health. In this study, a smartphone-ratiometric fiuorescence sensor for the ultrasensitive detection of OTA is developed based on a porphyrinic metal-organic framework and silica nanoparticle composite(Zr-MOF/Si NPs)away from the use of antibodies and aptamers. Due to the excellent recognition ability of Zr-MOF and good storage stability of Si NPs, OTA is detected by Zr-MOF/Si NPs with a wide linear range of 0.05–1000 ng/m L and low detection limit of 0.016 ng/m L. Moreover, the red–blue ratio values of the fiuorescence images are extracted through the smartphone color recognizer application with a limit of detection of 1.74 ng/m L, lower than the permissible content of OTA in cereal prescribed by World Health Organization. This sensing platform has been successfully applied in maize samples with superior repeatability and satisfactory recoveries, providing a novel way for simple and label-free analysis of OTA in cereal.
基金supported by the National Natural Science Foundation of China(Nos.32260247 and 22064010)the Natural Science Foundation of Jiangxi Province(Nos.20232BAB215071 and 20224BAB213009).
文摘Developing an accurate and visual sensing strategy for trace levels of fluoroquinolone residues that pose threat to food safety and human health is highly desired but remains challenging.Herein,a target selfcalibration ratiometric fluorescent sensing platform has been designed for sensitive visual detection of levofloxacin(LEV)based on fluorescent europium metal-organic framework(Eu-MOF)probe.Specifically,the Eu-MOF was facilely synthesized via directly mixing Eu^(3+)with 1,10-phenanthroline-2,9-dicarboxylic acid(PDA)ligand at room temperature,which exhibited well-stable red fluorescence at 612 nm.Upon the addition of target LEV,the significant fluorescence quenching from Eu^(3+)was observed owing to the inner filter effect between the Eu-MOF and LEV.While the intrinsic fluorescence for LEV at 462nm was gradually enhanced,thereby realizing the self-calibration ratiometric fluorescence responses to LEV.Through this strategy,LEV can be detected down to 27 nmol/L.Furthermore,a test paper-based Eu-MOF integrated with the smartphone assisted RGB color analysis was exploited for the quantitative monitoring of LEV through the multi-color changes from red to blue,thus achieved portable,convenient and visual detection of LEV in honey and milk samples.Therefore,the developed strategy could provide a useful tool for supporting the practical on-site test in food samples.
基金supported by the National Natural Science Foundation of China(No.22064014)the Science and Technology Development Plan Project of Lanzhou(No.2021–1-146)+2 种基金the Science and Technology Project of Gansu Province(Nos.21YF5FA071,21JR7RA538)the Industrial Support Programme for Higher Education Institutions Project(Nos.2023CYZC-69,2024CYCZ-05)the 2023 Gansu Provincial Key Talent Project(No.2023RCXM26)。
文摘The traditional nanozymes-based ratiometric fluorescence sensing platforms usually necessitate the supplementary addition of fluorescent probes,therefore greatly restricting its convenient and broad application.In this study,a highly sensitive and selective ratiometric fluorescence platform for alkaline phosphatase(ALP)detection was established,only employing Prussian blue(PB)nanozymes and a commercially available chromogen of o-phenylenediamine(OPD).PB nanozymes with remarkable peroxidaselike(POD-like)activity can effectively catalyze OPD chromogen to yield 2,3-diaminophenazine(OPDox)with an intense yellow fluorescence at 573 nm emission peak.Target ALP can facilitate ascorbic acid 2-phosphate(AAP)dephosphorylation to generate phosphate and ascorbic acid(AA).Significantly,both these two resultant hydrolysis products could effectively decrease the OPDox generation via a dualpath based inhibition on the PB nanozymes POD-like activity.On the other hand,the generated dehydroascorbic acid(DHAA)from AA oxidation would exclusively react with OPD chromogen to yield3-(dihydroxyethyl)furo[3,4-b]quinoxaline-1-one(DFQ)with a strong blue fluorescent signal at 434nm,which further providing a significant enhancement on the sensing selectivity of ALP detection.As a result,an increased yellow fluorescence of OPDox and decreased blue fluorescence of DFQ could be clearly observed with different ALP addition.A robust linear relationship between the fluorescence ratio of F_(434)/F_(573)and ALP activity ranging from 0.25U/L to 6U/L was obtained,with a low detection limit of 0.112 U/L.This proposed method demonstrates high sensitivity,excellent selectivity,cost-effectiveness,and operational simplicity,yet enabling an effective detection of ALP levels in human serum.
基金supported by the National Natural Science Foundation of China(No.22077044)the Natural Science Foundation of Hubei Province(No.2022CFA033).
文摘Gallstones are a common disease worldwide,often leading to obstruction and inflammatory complications,which seriously affect the quality of life of patients.Research has shown that gallstone disease is associated with ferroptosis,lipid droplets(LDs),and abnormal levels of nitric oxide(NO).Fluorescent probes provide a sensitive and convenient method for detecting important substances in life systems and diseases.However,so far,no fluorescent probes for NO and LDs in gallstone disease have been reported.In this work,an effective ratiometric fluorescent probe LR-NH was designed for the detection of NO in LDs.With an anthracimide fluorophore and a secondary amine as a response site for NO,LR-NH exhibits high selectivity,sensitivity,and attractive ratiometric capability in detecting NO.Importantly,it can target LDs and shows excellent imaging ability for NO in cells and ferroptosis.Moreover,LR-NH can target the gallbladder and image NO in gallstone disease models,providing a unique and unprecedented tool for studying NO in LDs and gallbladder.
基金Project supported by the Zhejiang Provincial Natural Science Foundation of China (LY24E020005)Industrial Key Projects of Jinhua City(2023-1-090)。
文摘Mixed-lanthanide(Ln) luminescent materials have important application values in ratiometric temperature sensing.Hydrogen-bonded organic frameworks(HOFs) offer a self-supporting network to conveniently tune multiple Ln^(3+)ions,but challenges still exist in material design.In this work,we selected two simple organic molecules as hydrogen-bonded building blocks,which are melamine(MA) and 2,5-pyridinedicarboxylic acid(PDC) owning the suitable energy to sensitize Tb^(3+)and Eu^(3+)ions.MA assists PDC molecules to support the network in a new HOF(MA-PDC),where the abundant carboxylic groups enable Ln^(3+)ions to combine with the HOF.Based on the effective energy transfer process,the emission of Tb^(3+)and Eu^(3+)from TbxEu1-x@MA-PDC(x=0.75,0.85,0.90) shows the obvious temperature dependence,which benefits ratiometric temperature detection.Taking Tb0.85Eu0.15@MA-PDC as an example,when temperature varies from 303 to 443 K,the intensity ratio exhibits distinct S-type response based on Mott-Seitz model.It also behaves good relative sensitivity(1.03%/K at 383 K),low temperature resolution(0.059 K) and large reproducibility(>96%).This work demonstrates that functionalization of a simple-component HOF with mixed Ln^(3+)ions is a fantastic strategy to develop novel ratiometric thermometers of both low cost and good performance.
基金Project supported by the National Natural Science Foundation of China (21771141)。
文摘A 12-metal Zn(Ⅱ)-Nd(Ⅲ) cluster 1(sizes:1.8 nm×2.0 nm×2.0 nm) was synthesized from a long-chain type Schiff base ligand.It displays ratiometric fluorescence response to neopterin(Neo) with high selectivity and sensitivity,which can be expressed by the equation I_(545)_(nm)/I_(1060)_(nm)=A·[Neo]^(2)+B·[Neo]+C.1 is used to quantitatively test Neo concentrations in fetal calf serum(FCS) and urine,and the recovery ranges are 98.57%-103.82% and 99.25%-103.50%,respectively,while the relative standard deviations(RSDs) are 7.89%-9.46% and 1.85%-4.16%,respectively.The limits of detection of 1 to Neo in FCS and urine are 0.034 and 0.021 μmol/L,respectively.
基金financially supported by the National Natural Science Foundation of China(Nos.22171069 and 21871075)the Educational Committee of Hebei Province(No.JZX2024012)the Tianjin Natural Science Foundation(No.23JCYBJC00800)。
文摘Purely organic room-temperature phosphorescence(RTP)and fluorescence dual-emission materials in aqueous solution have attracted growing attention.Herein,we report a fluorescencephosphorescence dual emission host-vip complex by simple assembly of cucurbit[8]uril(CB[8])and4-(4-bromophenyl)pyridinium derivative in water.Macrocyclic confinement and unique 1:2 host-vip structure could effectively inhibit non-radiative transition of the vip and the quenching of water molecule,thus induce effective RTP emission in water(τ_(RTP)=0.472 ms,Φ_(RTP)=1.37%).Specifically,based on competitive binding,this host-vip complex exhibits rapid ratiometric luminescent detection behavior to 3-nitrotyrosine,a specific biomarker of kidney injury,with a low limit of detection of 10.7 nmol/L.This work highlights the great potential of macrocyclic-confinement-derived RTP materials in biomarker detection,and will undoubtedly broaden the utilization scope of RTP.
基金supported by the National Natural Science Foundation of China(No.T2422004,22278059,22174009,and 22078047)Fundamental Research Funds for the Central Universities(No.DUT22LAB601,DUT22LAB608,and DUT24ZD119)the Outstanding Youth Foundation of Liaoning(No.2022-YQ-08).
文摘Lipid peroxidation(LPO)in foam cells is crucial for regulating atherosclerosis progression.It correlates with lipid uptake and the state of lipid droplets.In this study,we report a lipid droplet-targeted fluorescent LPO probe,Ld-LPO.It selectively responds to LPO,resulting in a significant fluorescence shift from 590 to 525 nm,enabling a ratiometric imaging of LPO in lipid droplets.Ld-LPO traces lipid droplets in foam cells,revealing a correlation between LPO and lysosomal engulfment.We found that lipid droplets engulfed by lysosomes exhibit higher LPO,attributed to low-density lipoprotein accumulation in lysosomes.Furthermore,Ld-LPO is compatible with dual-color flow cytometry,facilitating highthroughput analysis of LPO in foam cells.
基金Project supported by the National Natural Science Foundation of China(U23A20579)。
文摘Introduction of porosity and fluorescent properties into lanthanide metal-organic frameworks(MOFs)with rational design to achieve multifunctional use is of great significance from the energy and environmental viewpoint.In this study,a microporous Tb(Ⅲ)-based MOF with the formula of{[Tb2(oba)3(Phen)2](DMF)2(H2O)4}_n(1)was solvothermally prepared via using the mixed ligands of 1,10-phenanthroline(Phen)and 4,4'-oxybis(benzoic acid)(H_(2)oba)as organic connecters.The structural evaluation results indicate that complex 1 is composed of binuclear Tb2(CO_(2))6(Phen)2clusters which are extended by the oba^(2-)ligands to afford a two-fold interpenetrated framework with one-dimensional microporous channels along the b-axis.Gas sorption studies show that the activated 1 demonstrates a high ideal adsorption solution theory(IAST)sorption selectivity of 7.4 toward C_(2)H_(2)in a C_(2)H_(2)/CO_(2)gas mixture,which are further supported by the dynamic breakthrough experiments.The grand canonical Monte Carlo(GCMC)simulation results indicate that the synergistic effects of the H-bond interactions of C_(2)H_(2)with oba^(2-)ligands and the C-H…πinteractions with Phen ligand contribute to the strong binding of the framework toward C_(2)H_(2)molecules.What's more,the luminescent measurements reveal that the emission of 1 features both the characteristic peaks of Phen ligand and Tb(Ⅲ)ion,which could be further applied as a self-calibrating sensor for the Cr(Ⅵ)detection in water.To the best of our knowledge,complex 1 represents the first example of Tb-MOF holding such a high C_(2)H_(2)/CO_(2)selectivity together with ratiometric Cr(Ⅵ)detection performances.
基金supported by the National Natural Science Foundation of China(No.U1608222)the State Key Laboratory of Fine Chemicals(No.KF1606)the financial support from Fundamental Research Funds for the Central Universities of Central South University(No.2018zzts109)
文摘A ratiometric fluorescence probe,NClO,for the rapid and selective detection of HClO had been designed and synthesized based on a 1,8-naphthalimide derivative.Probe NClO displayed a red emission(λmax=615 nm).In the presence of HClO,the solution of probe NClO gave off a strong green fluorescence(λem,max=520 nm)with a rapid response(within seconds).This probe had been applied to image HClO in living cells and zebra fish.
基金financial support from the National Natural Science Foundation of China (No. 21205108)the Foundation for University Key Teacher by Henan Province (No. 2017GGJS007)+1 种基金China Postdoctoral Science Foundation (Nos. 2017M620302, 2018T110736)the Key Scientific Research Project in Universities of Henan Province (No. 19A150048)
文摘Intracellular pH plays a significant role in various biological processes, including cell proliferation,apoptosis, metabolism, enzyme activity and homeostasis. In this work, a novel design strategy for the preparation of pH responsive carbon dots(CDs-pH) for ratiometric intracellular imaging was reported. By using SciFinder database, fluorescent CDs-pH with the required p Kavalue of 6.84 were rationally designed, which is vital important for precise sensing of intracellular pH. As a result, the synthesized CDspH demonstrated robust ability to test pH fluctuations within the physiological range of 5.4-7.4. The CDspH was further utilized for fluorescent ratiometric imaging of pH in living HeLa cells, effectively avoided the influence of autofluorescence from native cellular species. Moreover, real-time monitoring of intracellular pH fluctuation under heat shock was successfully realized. This SciFinder-guided design strategy is simple and flexible, which has a great potential to be used for the development of other types of CDs for various applications.
基金This work was supported by the Hubei Provincial Department of Education Science and Technology Research Projects(No.Q20182704).
文摘The reactive oxygen species(ROS) are tightly associated with endoplasmic reticulum(ER) stress.Thus,the deep and visual insight of aberrant ROS fluctuations in the ER can help us better investigate the ER stress-associated pathology.In this work,a fluorescent probe ERC for HOCI detection in the ER based on phenothiazine-derived coumarin platform was developed.In the presence of HOCI,ERC exhibited an emission change from 609 nm to 503 nm within seconds.It also showed high sensitivity(0.44 μmol/L)and superb photostability.Significantly,ERC displayed low cytotoxicity,good cell membrane permeability,and appreciable ER-targetability.Ultimately,the probe was successfully utilized to image exogenous and endogenous HOCl in living cells and reveal the HOCI burst in cisplatin-treated cancer cells.
基金the National Natural Science Foundation of China(Nos.21672185 and 21867019)the"Youth Talent of WanRen Project"Foundation of Yunnan Province of China for the financial support"LianDa Scholar Project"and"Graduate Research and Innovation"Foundation of Yunnan Normal University(No.Ysdyjs2019122)。
文摘Iron is one of the essential trace elements in the human body.It plays an important role in human biology and pathology.Deregulation of iron levels in cells is associated with disease development.In this work,we synthesized a novel near-infrared intramolecular charge transfer(ICT)based ratiometric fluorescent probe to detect Fe^(2+),by using naphthalimide and indole moieties as building blocks.Our work showed that the radiometric probe has excellent selectivity,sensitivity and rapid response.Moreover,we could successfully perform real-time monitoring of Fe^(2+) in He La cells and C.elegans.
基金supported by the National Natural Science Foundation of China(No.U1608222)Special Fund for Agroscientific Research in the Public Interest of China(No.201503108)+1 种基金the Fundamental Research Funds for the Central Universities of Central South University(Nos.2019zzts438,2019zzts846)the State Key Laboratory of Chemo/Biosensing and Chemometrics(No.2016005)。
文摘We developed a merocyanine-based fluorescent probe,NEPB,for tracing hydrazine(N2 H4)in a ratiometric manner with large Stokes shifts and long emission wavelength.The fluorescence color of probe NEPB changed from green to yellow upon addition of hydrazine.Probe NEPB displayed high selectivity and sensitivity to hydrazine in solution,and could ratiometrically monitor N2 H4 in living cells and zebrafish with low cytotoxicity.
