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Yankee Group Ranks ZTE 2nd Best in CDMA Competitiveness
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《ZTE Communications》 2008年第2期68-68,共1页
ZTE Corporation announced on March 31, 2008 that it has been ranked second in terms of CDMA equipment competitiveness by the Yankee Group in a report entitled "ZTE Shines in CDMA Market" published by the res... ZTE Corporation announced on March 31, 2008 that it has been ranked second in terms of CDMA equipment competitiveness by the Yankee Group in a report entitled "ZTE Shines in CDMA Market" published by the research firm in February this year. According to the report, ZTE is the most competitive telecom vendor in China, India, emerging Asia-Pacific market, Africa and Eastern Europe. ZTE tallied the highest score in four out of seven categories the Yankee Group used to measure the CDMA competitiveness of each vendor. The seven key evaluation factors were price, technology, existing installed base, product portfolio, integration capability, government support and company culture. 展开更多
关键词 ZTE CDMA Yankee Group ranks ZTE 2nd Best in CDMA Competitiveness
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Effect of Temperature on the Molecular Weight Distribution in the Different Ranks of Coal during the On-Line Investigation of Coal Pyrolysis Gas Using Direct Photoionization Mass Spectroscopy
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作者 Makhosazana Mthembu Ralf Zimmermann +1 位作者 Thorsten Streibel Martin Sklorz 《International Journal of Clean Coal and Energy》 2015年第4期69-80,共12页
Coal pyrolysis gas from different ranks of coal was monitored on real time basis using photoionization mass spectroscopy. The molecular weight distribution of different products as a function of temperature from vario... Coal pyrolysis gas from different ranks of coal was monitored on real time basis using photoionization mass spectroscopy. The molecular weight distribution of different products as a function of temperature from various coal ranks studied was observed. It was noted that the release of different classes of compounds like phenols, alkenes, alkylated aromatics and aromatic skeletons was temperature dependent. For all the coal ranks at lower temperatures phenols were the main component, with alkenes and alkylated aromatics at slight higher temperatures and aromatic skeletons were released at the highest temperatures studied. 展开更多
关键词 COAL ranks PHOTOIONIZATION Temperature Effect Molecular WEIGHT Distribution COAL PYROLYSIS Gas
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Institute of Policy and Management: Aiming at the Ranks of World-class Think Tanks
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《Bulletin of the Chinese Academy of Sciences》 2002年第2期114-117,共4页
  Established on June 26, 1986 by the consolidation of four former CAS subordinates (namely,the Office of Policy Research, Division of Management Science, Editorial Department of the Journal of Dialectics of Nature,...   Established on June 26, 1986 by the consolidation of four former CAS subordinates (namely,the Office of Policy Research, Division of Management Science, Editorial Department of the Journal of Dialectics of Nature, and Section of Optimization and Overall Planning under the CAS Institute of Applied Mathematics), the Institute of Policy and Management (IPM), CAS, is characterized by interdisciplinary studies of social and natural sciences. It applies itself to theoretical, methodological and applied research into national strategies, policies and management for S&T development. It also provides consultancy services for government departments, local authorities and CAS on issues concerning S&T development, social and economic growth, S&T management and administration of enterprises.…… 展开更多
关键词 Institute of Policy and Management IPM Aiming at the ranks of World-class Think Tanks CAS World
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THE RANKS OF CYCLIC AND NEGACYCLIC CODES OVER THE FINITE RING R 被引量:9
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作者 Zhu Shixin Shi Minjia 《Journal of Electronics(China)》 2008年第1期97-101,共5页
The ranks of cyclic and negacyclic codes over the finite chain ring R as well as their minimal generating sets are defined, and then the expression forms we presented by studying the structures of cyclic and negacycli... The ranks of cyclic and negacyclic codes over the finite chain ring R as well as their minimal generating sets are defined, and then the expression forms we presented by studying the structures of cyclic and negacyclic codes over the finite chain ring R. Through the paper, it is assumed that the length of codes n can not be divided by the characteristic of R. 展开更多
关键词 Cyclic code RANK Minimal generating set
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Extremal ranks of the solution to a system of real quaternion matrix equations 被引量:1
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作者 俞绍文 王卿文 《Journal of Shanghai University(English Edition)》 CAS 2007年第3期229-232,共4页
In this paper, the maximal and minimal ranks of the solution to a system of matrix equations over H, the real quaternion algebra, were derived. A previous known result could be regarded as a special case of the new re... In this paper, the maximal and minimal ranks of the solution to a system of matrix equations over H, the real quaternion algebra, were derived. A previous known result could be regarded as a special case of the new result. 展开更多
关键词 system of matrix equations SOLUTION minimal rank maximal rank generalized inverse
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Development of Data Mining Models Based on Features Ranks Voting (FRV)
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作者 Mofreh A.Hogo 《Computers, Materials & Continua》 SCIE EI 2022年第11期2947-2966,共20页
Data size plays a significant role in the design and the performance of data mining models.A good feature selection algorithm reduces the problems of big data size and noise due to data redundancy.Features selection a... Data size plays a significant role in the design and the performance of data mining models.A good feature selection algorithm reduces the problems of big data size and noise due to data redundancy.Features selection algorithms aim at selecting the best features and eliminating unnecessary ones,which in turn simplifies the structure of the data mining model as well as increases its performance.This paper introduces a robust features selection algorithm,named Features Ranking Voting Algorithm FRV.It merges the benefits of the different features selection algorithms to specify the features ranks in the dataset correctly and robustly;based on the feature ranks and voting algorithm.The FRV comprises of three different proposed techniques to select the minimum best feature set,the forward voting technique to select the best high ranks features,the backward voting technique,which drops the low ranks features(low importance feature),and the third technique merges the outputs from the forward and backward techniques to maximize the robustness of the selected features set.Different data mining models were built using obtained selected features sets from applying the proposed FVR on different datasets;to evaluate the success behavior of the proposed FRV.The high performance of these data mining models reflects the success of the proposed FRV algorithm.The FRV performance is compared with other features selection algorithms.It successes to develop data mining models for the Hungarian CAD dataset with Acc.of 96.8%,and with Acc.of 96%for the Z-Alizadeh Sani CAD dataset compared with 83.94%and 92.56%respectively in[48]. 展开更多
关键词 EVALUATOR features selection data mining FORWARD BACKWARD VOTING feature rank
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Context Based Adoption of Ranking and Indexing Measures for Cricket Team Ranks
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作者 Raja Sher Afgun Usmani Syed Muhammad Saqlain Shah +4 位作者 Muhammad Sher Ramzan Abdullah Saad A.L-Malaise AL-Ghamdi Anwar Ghani Imran Khan Farrukh Saleem 《Computers, Materials & Continua》 SCIE EI 2020年第11期1113-1136,共24页
There is an international cricket governing body that ranks the expertise of all the cricket playing nations,known as the International Cricket Council(ICC).The ranking system followed by the ICC relies on the winning... There is an international cricket governing body that ranks the expertise of all the cricket playing nations,known as the International Cricket Council(ICC).The ranking system followed by the ICC relies on the winnings and defeats of the teams.The model used by the ICC to implement rankings is deficient in certain key respects.It ignores key factors like winning margin and strength of the opposition.Various measures of the ranking concept are presented in this research.The proposed methods adopt the concepts of h-Index and PageRank for presenting more comprehensive ranking metrics.The proposed approaches not only rank the teams on their losing/winning stats but also take into consideration the margin of winning and the quality of the opposition.Three cricket team ranking techniques are presented i.e.,(1)Cricket Team-Index(ct-index),(2)Cricket Team Rank(CTR)and(3)Weighted Cricket Team Rank(WCTR).The proposed metrics are validated through the collection of cricket dataset,extracted from Cricinfo,having instances for all the three formats of the game i.e.,T20 International(T20i),One Day International(ODI)and Test matches.The comparative analysis between the proposed and existing techniques,for all the three formats,is presented as well. 展开更多
关键词 INDEXING RANKING cricket teams runs and wickets
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Ranks of Repeated-Root Constacyclic Code
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作者 LI Hongju DING Jian TANG Yongsheng 《Wuhan University Journal of Natural Sciences》 CAS CSCD 2016年第5期453-460,共8页
By the discussion of division in F2m[u]/〈u4〉,the minimal spanning set and the rank of a(1+u+u2)-constacyclic code with an arbitrary length N=2en over F2m[u]/〈u4〉 are determined based on the factorization of(x... By the discussion of division in F2m[u]/〈u4〉,the minimal spanning set and the rank of a(1+u+u2)-constacyclic code with an arbitrary length N=2en over F2m[u]/〈u4〉 are determined based on the factorization of(xn-1) over F2m. 展开更多
关键词 constacyclic codes RANK the minimal spanning set
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On the Complexity of Finding Tensor Ranks
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作者 Mohsen Aliabadi Shmuel Friedland 《Communications on Applied Mathematics and Computation》 2021年第2期281-289,共9页
The purpose of this note is to give a linear algebra algorithm to find out if a rank of a given tensor over a field F is at most k over the algebraic closure of F,where K is a given positive integer.We estimate the ar... The purpose of this note is to give a linear algebra algorithm to find out if a rank of a given tensor over a field F is at most k over the algebraic closure of F,where K is a given positive integer.We estimate the arithmetic complexity of our algorithm. 展开更多
关键词 Gauss elimination Homogeneous polynomial NP-HARDNESS Symmetric tensor Tensor rank
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Distance two Heegaard splittings,JSJ decompositions and ranks of 3-manifolds
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作者 Wenjie Diao Yanqing Zou 《Science China Mathematics》 2025年第6期1431-1442,共12页
For any pair of integers g and n with g≥3 and 1≤n≤g,we build a 3-manifold with a distance-2,genus-g Heegaard splitting so that(1)it contains n pairwise disjoint and nonisotopic essential tori;(2)after it is cut ope... For any pair of integers g and n with g≥3 and 1≤n≤g,we build a 3-manifold with a distance-2,genus-g Heegaard splitting so that(1)it contains n pairwise disjoint and nonisotopic essential tori;(2)after it is cut open along these tori,one resulting piece is hyperbolic while the others are small Seifert fibered spaces;(3)it provides a substantial result to the rank versus genus problem.These generalize a result in Qiu and Zou(2019). 展开更多
关键词 Heegaard distance Heegaard genus RANK hyperbolic 3-manifold curve complex JSJ decomposition
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右归丸活性成分对成骨-破骨共育体系Wnt/β-catenin、OPG/RANKL/RANK通路相关因子mRNA表达的影响
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作者 章建华 裘佳韦 +2 位作者 商佳璐 叶怡馨 尹华 《中华中医药杂志》 北大核心 2025年第7期3456-3463,共8页
目的:明确右归丸活性成分乌头碱类、松脂醇葡萄糖苷、肉桂酸等对成骨(OB)-破骨(OC)共育体系Wnt/β-catenin、OPG/RANKL/RANK通路相关因子LRP6、LRP5、β-catenin等mRNA表达的调控作用。方法:分别用MC3T3-E1和RAW264.7诱导培养OB和OC并... 目的:明确右归丸活性成分乌头碱类、松脂醇葡萄糖苷、肉桂酸等对成骨(OB)-破骨(OC)共育体系Wnt/β-catenin、OPG/RANKL/RANK通路相关因子LRP6、LRP5、β-catenin等mRNA表达的调控作用。方法:分别用MC3T3-E1和RAW264.7诱导培养OB和OC并进行鉴定,以Transwell共培养建立OB-OC共育体系;分别制备右归丸活性成分乌头碱、次乌头碱、新乌头碱、苯甲酰乌头原碱、苯甲酰次乌头原碱、苯甲酰新乌头原碱、松脂醇二葡萄糖苷、肉桂酸的单体干预液,并采用RT-qPCR法检测细胞中LRP6、LRP5、β-catenin、TCF7、Fzd2、OPG、TRAF6、NFATc1、c-Fos、MMP9、CTSK、Car2 mRNA的表达。结果:成功诱导OB和OC,并建立OB-OC共育体系;右归丸活性成分中乌头碱、次乌头碱、新乌头碱、苯甲酰乌头原碱、苯甲酰新乌头原碱、松脂醇二葡萄糖苷、肉桂酸能上调OB-OC共育体系中Wnt/β-catenin通路LRP6、LRP5、β-catenin、TCF7、Fzd2 mRNA表达水平;次乌头碱、新乌头碱、苯甲酰乌头原碱、苯甲酰新乌头原碱、松脂醇二葡萄糖能上调OPG/RANKL/RANK通路OPG mRNA表达水平;乌头碱、次乌头碱、新乌头碱、苯甲酰乌头原碱、苯甲酰次乌头原碱、苯甲酰新乌头原碱、松脂醇二葡萄糖苷、肉桂酸能抑制OPG/RANKL/RANK通路TRAF6、NFATc1、c-Fos、MMP9、CTSK、Car2 mRNA水平的表达。结论:右归丸活性成分可以通过调控Wnt/β-catenin、OPG/RANKL/RANK通路相关因子的基因表达,促进骨形成,抑制骨吸收,重建骨代谢平衡,起到治疗骨质疏松肾阳虚证的作用。 展开更多
关键词 右归丸活性成分 成骨-破骨共育体系 Wnt/β-catenin通路 OPG/RANKL/RANK通路 mRNA
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金匮肾气丸对糖尿病肾病性骨质疏松大鼠的影响及机制
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作者 丁文君 沈明霞 高永蕊 《中国药房》 北大核心 2025年第16期2000-2004,共5页
目的探讨金匮肾气丸对糖尿病肾病性骨质疏松(DNOP)大鼠的影响及潜在机制。方法将大鼠随机分为空白对照组,对照组,模型组,金匮肾气丸低、中、高剂量组(0.62、1.24、2.48 g/kg)和阳性对照组(地舒单抗3 mg/kg),每组12只。除空白对照组和对... 目的探讨金匮肾气丸对糖尿病肾病性骨质疏松(DNOP)大鼠的影响及潜在机制。方法将大鼠随机分为空白对照组,对照组,模型组,金匮肾气丸低、中、高剂量组(0.62、1.24、2.48 g/kg)和阳性对照组(地舒单抗3 mg/kg),每组12只。除空白对照组和对照组外,其余各组均以高糖高脂饲料、3%果糖溶液喂养+链脲佐菌素溶液腹腔注射的方式构建DNOP大鼠模型。造模成功后第2天,金匮肾气丸低、中、高剂量组和阳性对照组大鼠分别灌胃或皮下注射相应药液,每天1次,连续8周。末次给药后,检测各组大鼠的骨密度(BMD)、血清骨代谢相关指标[骨钙素(OC)、钙、磷、碱性磷酸酶(ALP)、抗酒石酸酸性磷酸酶(TRAP)],观察其右股骨组织病理学改变,检测其左股骨组织中核因子κB受体激活蛋白配体(RANKL)/核因子κB受体激活蛋白(RANK)/护骨因子(OPG)信号通路相关mRNA及蛋白的表达情况。结果与空白对照组、对照组比较,模型组大鼠骨小梁断裂及结构受损严重,骨组织间隙明显;BMD、ALP活性、OPG mRNA及蛋白的表达均显著降低或下调(P<0.05);OC、钙、磷水平,TRAP活性,RANKL、RANK mRNA及蛋白的表达均显著升高或上调(P<0.05)。与模型组比较,金匮肾气丸各剂量组大鼠骨小梁数有所增加,骨小梁排列趋于整齐、致密;上述指标普遍回调,且有一定的剂量依赖性(P<0.05)。结论金匮肾气丸可促进DNOP大鼠骨生成,调节骨代谢平衡;上述作用可能与下调RANKL、RANK表达,上调OPG表达有关。 展开更多
关键词 金匮肾气丸 糖尿病肾病性骨质疏松 骨代谢 RANKL/RANK/OPG信号通路
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基于自然语言处理的“双碳”政策知识图谱构建及应用
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作者 吕涛 王青山 +3 位作者 张紫玉 吴昱磊 周孜柔 王洛 《煤炭经济研究》 2025年第2期122-132,共11页
“双碳”政策具有发布数量多、覆盖范围广、内容复杂多样等特点,现有的呈现方式难以满足知识检索和内在分析的需求。以2953条“双碳”政策文本为数据源,提出了一种基于自然语言处理的“双碳”政策知识图谱构建方法,首先构建了知识图谱... “双碳”政策具有发布数量多、覆盖范围广、内容复杂多样等特点,现有的呈现方式难以满足知识检索和内在分析的需求。以2953条“双碳”政策文本为数据源,提出了一种基于自然语言处理的“双碳”政策知识图谱构建方法,首先构建了知识图谱模式层,定义了“双碳”政策实体、属性和关系,之后采用Text Rank关键词抽取、LDA主题建模等算法提取政策实体、属性及关系,构建了知识图谱数据层,最终将〈实体,关系,实体〉三元组存入Neo4j图数据库,形成“双碳”政策知识图谱。所构建的知识图谱包含2048个实体节点和32336条关系,可通过Cypher语言实现不同细粒度政策实体和关系的关联查询与可视化,挖掘“双碳”政策中的关键语义信息和政策热点,还可为智能服务提供语义增强功能,提高“双碳”政策推荐系统的效率和政策问答系统的准确度。 展开更多
关键词 “双碳”政策 知识图谱 自然语言处理 Neo4j LDA Text Rank
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调控RANKL/RANK/TLR4信号通路对脓毒症相关急性肾损伤的影响
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作者 牛新荣 李辉 陈蔚霖 《河北医药》 2025年第3期381-385,390,共6页
目的探讨核因子-κB受体活化因子配体(RANKL)/核因子-κB受体激活因子(RANK)/toll样受体4(TLR4)信号通路在脓毒症相关急性肾损伤(SA-AKI)炎性反应中的作用及可能的机制。方法将24只雄性C57BL/6小鼠按随机数字表法分为假手术组(S组)、盲... 目的探讨核因子-κB受体活化因子配体(RANKL)/核因子-κB受体激活因子(RANK)/toll样受体4(TLR4)信号通路在脓毒症相关急性肾损伤(SA-AKI)炎性反应中的作用及可能的机制。方法将24只雄性C57BL/6小鼠按随机数字表法分为假手术组(S组)、盲肠结扎穿孔术(CLP)诱导的脓毒症模型组(C组)、重组RANKL预处理组(R组)、抗RANKL预处理组(A组),每组6只。术前2 h CLP+重组RANKL组予以腹腔注射重组RANKL,CLP+抗RANKL组给予腹腔注射抗RANKL,制模后24 h采血并处死小鼠留取肾脏组织,用肌酐(Scr)及尿素氮(BUN)检测试剂盒检测血Scr、BUN水平,酶联免疫吸附测定(ELISA)法检测血清中IL-6、IL-1β、TNF-α水平,苏木精-伊红染色法(HE染色)观察肾脏组织病理学变化,反转录聚合酶链反应(RT-qPCR)及蛋白印迹法(Western blotting)检测肾脏组织中RANKL、RANK、TLR4 mRNA及蛋白表达水平。结果与假手术组比较,脓毒症组血清Scr、BUN、IL-1β、TNF-α、IL-6明显升高,肾脏组织病理学结果示肾小球部分缺血皱缩,球囊间隙扩大,肾小管上皮细胞坏死脱落于管腔中,管腔扩大,肾间质水肿,炎性细胞浸润,肾脏病理学评分明显升高,肾脏组织中RANKL的mRNA及蛋白表达均明显降低,而RANK和TLR4的mRNA及蛋白表达均明显升高,差异有统计学意义(均P<0.05);与脓毒症组比较,重组RANKL预处理组Scr、BUN、IL-1β、TNF-α、IL-6水平及病理学评分均明显降低,肾脏组织病理学结果示肾脏损害明显缓解,肾脏组织中RANKL的mRNA及蛋白表达均明显升高,而RANK和TLR4的mRNA及蛋白表达均明显下降,差异有统计学意义(均P<0.05);而抗RANKL预处理组肾功能及炎性因子水平有所升高,肾脏损伤程度加重,肾脏组织中RANKL的mRNA及蛋白表达均明显降低,RANK和TLR4的mRNA及蛋白表达均明显升高,差异有统计学意义(均P<0.05)。结论在SA-AKI的小鼠模型中,通过增加RANKL/RANK/TLR4信号通路的信号转导可减轻炎性反应,从而对CLP引起的急性肾损伤起保护作用。 展开更多
关键词 脓毒症 急性肾损伤 RANKL/RANK/TLR4信号通路 炎性反应
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CT灌注成像参数和血清CyPA水平与急性脑梗死患者预后不良的相关性
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作者 李飞 《中国民康医学》 2025年第13期125-127,131,共4页
目的:分析电子计算机断层扫描灌注成像(CTP)参数和血清亲环蛋白A(CyPA)水平与急性脑梗死(ACI)患者预后不良的相关性。方法:回顾性分析2021年11月至2023年11月该院收治的65例ACI患者的临床资料,设为研究组,另收集同期65名体检健康者设为... 目的:分析电子计算机断层扫描灌注成像(CTP)参数和血清亲环蛋白A(CyPA)水平与急性脑梗死(ACI)患者预后不良的相关性。方法:回顾性分析2021年11月至2023年11月该院收治的65例ACI患者的临床资料,设为研究组,另收集同期65名体检健康者设为对照组。统计ACI患者预后情况[改良Ranking量表(mRS)],并依据预后将其分为预后良好者和预后不良者,比较两组、不同预后患者CTP参数[平均通过时间(MTT)、脑血流量(CBF)、达峰时间(TTP)、脑血容量(CBV)]和CyPA水平,并分析CTP参数和CyPA水平与ACI患者预后情况的相关性。结果:65例ACI患者预后不良25例,占38.46%;预后良好40例,占61.54%;研究组CBF、CBV水平均低于对照组,MTT、TTP、CyPA水平均高于对照组,差异有统计学意义(P<0.05);预后不良者MTT、TTP、CyPA水平均高于预后良好者,CBF、CBV水平均低于预后良好者,差异有统计学意义(P<0.05);Pearson相关性分析结果显示,CBF、CBV水平与ACI患者预后不良均呈负相关(r<0,P<0.05);MTT、TTP、CyPA水平与ACI患者预后不良均呈正相关(r>0,P<0.05)。结论:CBF、CBV水平与ACI患者预后不良均呈负相关,MTT、TTP、CyPA水平与ACI患者预后不良均呈正相关。 展开更多
关键词 电子计算机断层扫描灌注成像 亲环蛋白A 急性脑梗死 改良Ranking量表 相关性
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Effects of individual and group factors on the social relationships of Budgerigars
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作者 Xueqi Zhao Jiangping Yu +4 位作者 Jie Su Manxi Lu Chunguang Dong Xiyu Wang Haitao Wang 《Avian Research》 2025年第4期706-712,共7页
Social animals often form dynamic relationships with group members,which have been associated with increased social learning,survival and reproductive success.Social relationships can be shaped by both group and indiv... Social animals often form dynamic relationships with group members,which have been associated with increased social learning,survival and reproductive success.Social relationships can be shaped by both group and individual factors;however,few studies have addressed their combined impact.In this study,we aimed to examine whether group factors and individual factors jointly affect social relations.We selected Budgerigars(Melopsittacus undulatus)as the focal species to investigate the influence of sex,personality,and body length as well as sex ratio and group size on social relationships.The results showed that the birds in 3-individual groups had higher aggression network weighted degree values than those in 5-individual groups.Individuals within opposite-sex groups showed higher levels of aggressive and affiliative interactions than those in same-sex groups.Additionally,females attained higher social ranks despite exhibiting significantly lower aggression behaviors than males.Individuals with longer body lengths exhibited higher aggression network weighted degree values.Our results suggest that group factors primarily influence the social networks,while individual factors play important roles in shaping the social relationships. 展开更多
关键词 Group size Sex ratio Social network Social ranks
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肉苁蓉苷A通过JNK/MAPK通路抑制破骨细胞活性 被引量:4
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作者 李岳尧 张民 杨家驹 《中国组织工程研究》 CAS 北大核心 2025年第6期1144-1151,共8页
背景:研究证实肉苁蓉苷A具有抗炎、抗氧化和抗骨质疏松的作用,但其对破骨细胞分化和功能的影响及潜在的机制尚缺少研究。目的:探讨肉苁蓉苷A对核因子κB受体活化因子配体诱导的体外破骨细胞分化和骨吸收功能的影响及作用机制。方法:提... 背景:研究证实肉苁蓉苷A具有抗炎、抗氧化和抗骨质疏松的作用,但其对破骨细胞分化和功能的影响及潜在的机制尚缺少研究。目的:探讨肉苁蓉苷A对核因子κB受体活化因子配体诱导的体外破骨细胞分化和骨吸收功能的影响及作用机制。方法:提取与培养4-6周龄C57BL/6小鼠股骨和胫骨中的骨髓巨噬细胞,采用CCK-8毒性实验检验不同浓度的肉苁蓉苷A(5,10,20,40,80,160μmol/L)对骨髓巨噬细胞的毒性,抗酒石酸酸性磷酸酶染色观察不同浓度的肉苁蓉苷A干预破骨细胞的分化情况,确定药物的有效干预浓度。将实验分为阳性对照组、肉苁蓉苷A低、中、高剂量组(40,80,160μmol/L),细胞贴壁后各组均加入50 ng/mL的核因子κB受体活化因子配体诱导破骨细胞分化,肉苁蓉苷A低、中、高剂量组分别加入相应剂量的肉苁蓉苷A进行干预。采用肌动蛋白环鬼笔环肽染色和DAPI染色检测肉苁蓉苷A对破骨细胞形成的影响;骨磨片甲苯胺蓝染色观察肉苁蓉苷A对破骨细胞骨吸收功能的影响;Western blot检测JNK/MAPK通路上下游蛋白的表达情况;RT-qPCR检测抗酒石酸酸性磷酸酶、DC-STAMP、Nfatc-1、Ctsk和c-Fos等破骨细胞分化和骨吸收功能相关基因的表达情况。结果与结论:①抗酒石酸酸性磷酸酶染色、肌动蛋白环染色和骨陷窝甲苯胺蓝染色结果表明,与阳性对照组相比,肉苁蓉苷A对核因子κB受体活化因子配体诱导的体外破骨细胞的分化和骨吸收功能具有呈剂量依赖性的显著抑制作用;②RT-qPCR结果表明,与阳性对照组相比,肉苁蓉苷A高剂量组和低剂量组的抗酒石酸酸性磷酸酶、DC-STAMP、Nfatc-1、Ctsk和c-Fos等的mRNA表达量均显著降低,且肉苁蓉苷A高剂量组的降低效果更加显著;③Western blot结果显示,高剂量肉苁蓉苷A干预10,20,30和60 min时显著抑制p-JNK蛋白的表达;与阳性对照组相比,肉苁蓉苷A低、中、高剂量组显著抑制Nfatc1和c-Fos蛋白的表达,且呈剂量依赖性;④结果说明,肉苁蓉苷A能够通过降低p-JNK蛋白水平,抑制MAPK通路的激活和破骨细胞关键基因的表达,进而抑制破骨细胞的形成和骨吸收功能。 展开更多
关键词 肉苁蓉苷A 破骨细胞 MAPK JNK RANKL RANK 骨质疏松 苯乙醇苷
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六味地黄丸对糖尿病肾病合并骨质疏松大鼠的作用及机制 被引量:1
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作者 丁文君 王静 +1 位作者 沈明霞 高永蕊 《南开大学学报(自然科学版)》 北大核心 2025年第1期54-60,共7页
建立糖尿病肾病合并骨质疏松大鼠模型,分为模型组、L-六味地黄丸组(0.75 g/kg)、M-六味地黄丸组(1.5 g/kg)、H-六味地黄丸组(3.0 g/kg)、H-六味地黄丸+RANKL重组蛋白(H-六味地黄丸+RANKL组)(3.0 g/kg六味地黄丸+2.0 mg/kg RANKL重组蛋... 建立糖尿病肾病合并骨质疏松大鼠模型,分为模型组、L-六味地黄丸组(0.75 g/kg)、M-六味地黄丸组(1.5 g/kg)、H-六味地黄丸组(3.0 g/kg)、H-六味地黄丸+RANKL重组蛋白(H-六味地黄丸+RANKL组)(3.0 g/kg六味地黄丸+2.0 mg/kg RANKL重组蛋白),正常组给予普通饲料以及饮用自来水.随后对大鼠进行糖尿病及骨代谢相关指标检测;ELISA法检测IL-6、IL-1β、TNF-α水平;HE检测股骨组织病理学变化.Western blot检测RANKL/RANK/OPG通路相关蛋白表达.探讨六味地黄丸调节RANKL/RANK/OPG信号通路对糖尿病肾病合并骨质疏松大鼠的作用及机制.结果显示与正常组相比,模型组骨小梁稀疏、间隙变大、数量减少,骨髓腔增大,FBG、HOMAIR、TG、TC、IL-6、IL-1β、TNF-α水平、肾肥大指数及RANKL、RANK蛋白表达升高,FINS、血磷、血钙水平及OPG蛋白表达降低(P<0.05);与模型组比较,L-六味地黄丸组、M-六味地黄丸组、H-六味地黄丸组FBG、HOMA-IR、TG、TC、IL-6、IL-1β、TNF-α水平、肾肥大指数及RANKL、RANK蛋白表达降低,骨小梁数量、FINS、血磷、血钙水平及OPG蛋白表达升高,不同剂量六味地黄丸组间存在统计学差异(P<0.05);与H-六味地黄丸组比较,H-六味地黄丸+RANKL组FBG、HOMA-IR、TG、TC、IL-6、IL-1β、TNF-α水平、肾肥大指数及RANKL、RANK蛋白表达升高,骨小梁数量、FINS、血磷、血钙水平及OPG蛋白表达降低(P<0.05).表明六味地黄丸通过调节RANKL/RANK/OPG信号通路改善糖尿病肾病合并骨质疏松大鼠症状. 展开更多
关键词 六味地黄丸 RANKL/RANK/OPG信号通路 糖尿病肾病 骨质疏松
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基于脾肾相关理论研究尾悬吊大鼠骨丢失的肠杆菌调控机制
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作者 宋采秋 刘昱麟 +5 位作者 韩行 王萌 张红梅 卢健 张林 谢鑫 《时珍国医国药》 北大核心 2025年第7期1377-1382,共6页
目的 研究补肾健脾方调节肠道菌群发挥抗骨丢失的作用机制。方法 将雌雄各30只大鼠,随机分为空白对照组、悬吊组、补肾健脾方低、中、高剂量组。除空白组外,其他组采用尾部悬吊法复制模型。运用双能X线骨密度仪检测股骨骨密度(BMD),HE... 目的 研究补肾健脾方调节肠道菌群发挥抗骨丢失的作用机制。方法 将雌雄各30只大鼠,随机分为空白对照组、悬吊组、补肾健脾方低、中、高剂量组。除空白组外,其他组采用尾部悬吊法复制模型。运用双能X线骨密度仪检测股骨骨密度(BMD),HE染色观察小肠组织形态,16Sr RNA测序分析大鼠肠道菌群,ELISA法检测血清中巨噬细胞集落刺激因子(M-CSF)及脂多糖(LPS)含量,Western blot法检测股骨中骨保护素(OPG)、核因子κB受体激活因子(RANK)、核因子κB受体激活因子配体(RANKL)的蛋白表达水平。结果 与空白对照组相比,悬吊组大鼠股骨骨密度明显降低(P<0.01),LPS、M-CSF、RANK、RANKL表达水平明显增加,OPG蛋白表达水平明显降低(P<0.01),肠杆菌丰度明显增高(P<0.05),小肠肠黏膜结构不完整。与悬吊组相比,中药各剂量组股骨骨矿物密度(BMD)明显增加(P<0.01),LPS、M-CSF、RANK、RANKL表达下降(P<0.05),OPG蛋白表达量上调(P<0.05),肠杆菌丰度降低,小肠黏膜结构较为完整。结论 补肾健脾方防治尾部悬吊大鼠骨丢失的机制与下调肠杆菌的丰度,进而抑制OPG/RANK/RANKL信号通路,减少骨吸收有关。 展开更多
关键词 模拟失重 补肾健脾方 脾肾相关 肠道菌群 骨丢失 OPG/RANK/RANKL
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OPG/RANK/RANKL信号通路在骨质疏松症中的作用及中医药干预研究进展
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作者 鄢裕 元宝华 +4 位作者 董伟 武忱 匡志平 杨琛 卢卫忠 《中国骨质疏松杂志》 北大核心 2025年第11期1704-1711,共8页
骨质疏松症由于其严重的致病后果同时较高的发病率已经成为我国老年人的头号健康潜在威胁,而OPG/RANK/RANKL信号通路主要通过影响破骨细胞分化成熟,从而在骨质疏松症的发病机制中占据了重要地位,是许多抗骨质疏松药品开发的重要研究靶... 骨质疏松症由于其严重的致病后果同时较高的发病率已经成为我国老年人的头号健康潜在威胁,而OPG/RANK/RANKL信号通路主要通过影响破骨细胞分化成熟,从而在骨质疏松症的发病机制中占据了重要地位,是许多抗骨质疏松药品开发的重要研究靶点之一。为了探究OPG/RANK/RANKL信号通路在骨质疏松症中的作用机制及中医药干预现状和进展,通过CNKI、WANFANG DATE、中华医学期刊全文数据库、Web of Science、Pub Med数据库,以骨质疏松症、OPG/RANK/RANKL、中药和中药方剂为关键词检索近10年相关文献报道,结果显示许多中药及其有效成分和中药方剂都可通过调控OPG/RANK/RANKL信号通路抑制破骨细胞,改善骨质疏松症,增加骨强度,提示中药及其有效成分和中药方剂能有效防治骨质疏松症,同时在药物研发方面具有极大的潜力和应用前景。 展开更多
关键词 OPG/RANK/RANKL信号通路 骨质疏松症 作用机制 中医中药
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