Objective:To observe the intervention effect of clinical nursing pathway on patients receiving intravenous therapy via peripheral mid-long catheters.Methods:Ninety patients receiving intravenous therapy via mid-long c...Objective:To observe the intervention effect of clinical nursing pathway on patients receiving intravenous therapy via peripheral mid-long catheters.Methods:Ninety patients receiving intravenous therapy via mid-long catheters in the hospital from March 2024 to March 2025 were selected and randomly divided into a control group and an observation group,with 45 patients in each group.The control group received routine nursing intervention,while the observation group received clinical nursing pathway intervention.The nursing risk events,catheter indwelling time,and nursing satisfaction were evaluated in both groups.Results:The observation group had a shorter catheter indwelling time,fewer risk events during intravenous therapy(total incidence rate of 2.22%),and higher nursing satisfaction.Additionally,negative emotional feedback was reduced,and the SAS score(31.02±1.12)was lower in the observation group compared to the control group.There were significant differences between the two groups in all indicators(P<0.05).Conclusion:Applying the clinical nursing pathway during intravenous therapy via peripheral mid-long catheters can help ensure infusion safety,improve unstable emotional states,and promote high-quality service standards.展开更多
Objective To investigate the effects of acupuncture on the neurotransmitter levels and gut microbiota in a mouse model of Tourette syndrome(TS).Methods Thirty-six male C57/BL6 mice were randomly divided into 4 groups ...Objective To investigate the effects of acupuncture on the neurotransmitter levels and gut microbiota in a mouse model of Tourette syndrome(TS).Methods Thirty-six male C57/BL6 mice were randomly divided into 4 groups using a random number table method:3,3’-iminodipropionitrile(IDPN)group,control group,acupuncture group,and tiapride group,with 9 mice in each group.In the IDPN group,acupuncture group,and tiapride group,mice received daily intraperitoneal injections of IDPN(300 mg/kg body weight)for 7 consecutive days to induce stereotyped behaviors.Subsequently,in the acupuncture intervention group,standardized acupuncture treatment was administered for 14 consecutive days to IDPN-induced TS model mice.The selected acupoints included Baihui(DU 20),Yintang(DU 29),Waiguan(SJ 5),and Zulinqi(GB 41).In the tiapride group,mice were administered tiapride(50 mg/kg body weight)via oral gavage daily for 14 consecutive days.The control group,IDPN group,and acupuncture group received the same volume of saline orally for 14 consecutive days.Stereotypic behaviors were quantified through behavioral assessments.Neurotransmitter levels,including dopamine(DA),glutamate(Glu),and aspartate(ASP)in striatal tissue were measured using enzyme-linked immunosorbent assay.Dopamine transporter(DAT)expression levels were additionally quantified through quantitative polymerase chain reaction(qPCR).Gut microbial composition was analyzed through 16S ribosomal RNA gene sequencing,while metabolic profiling was conducted using liquid chromatography-mass spectrometry(LC-MS).展开更多
Objective To study the action mechanism of Wenshen Tongdu Recipe(WSTDR)inpromoting microvascular endothelial cells(MECs)phagocytosis and clearing myelin debris by reducing iron overload phenomenon after spinal cord in...Objective To study the action mechanism of Wenshen Tongdu Recipe(WSTDR)inpromoting microvascular endothelial cells(MECs)phagocytosis and clearing myelin debris by reducing iron overload phenomenon after spinal cord injury,thereby improving the infiammatory microenvironment after spinal cord injury.Methods Fifty C57BL/6 mice were divided into 5 groups by random number table method:sham operation group,model group,deferoxamine(DFO)group,WSTDR group,WSTDR+DFO group,10 mice in each group.A mouse model of spinal cord injury was prepared by modified Allen's method.The pathological changes of the spinal cord were observed by HE staining and Nissl staining.The iron ion content in serum was determined by the iron ion colorimetric assay kit.The expression levels of monocyte chemoattractant protein-1(MCP-1),interleukin(IL)-6,IL-1β,and tumor necrosis factor-α(TNF-α)in the homogenate and serum of the spinal cord were detected by ELISA.The phagocytosis and clearance of myelin debris by MECs in the spinal cord were observed by 3D laser confocal.Results One mouse died in the model group,DFO group,and WSTDR group,respectively.The results of HE staining showed that compared with the model group,spinal cord cavities were reduced with nerve fiber disorder in DFO group and WSTDR group.Nissl staining analysis showed that compared with the model group,Nissl morphology and neuronal pyknosis were improved in DFO group and WSTDR group.Ferric ion colorimetric assay showed that compared with the model group,serum iron ion concentration were significantly reduced in DFO group and WSTDR group(P<0.01).ELISA results showed that compared with the sham-operation group,the levels of MCP-1,IL-6,IL-1β,and TNF-αin serum and spinal cord tissues significantly increased in the model group(P<0.01).Compared with the model group,serum levels of MCP-1,IL-6,IL-1β,and TNF-αwere significantly lowered in DFO group and WSTDR group(P<0.05,P<0.01).Serum concentrations of MCP-1 and IL-1βwere significantly lowered in WSTDR+DFO group(P<0.01).Compared with WSTDR group,serum concentrations of MCP-1,IL-6,anddIL-1βwere significantly improved in WSTDR+DFO group(P<0.01).Compared with the model group,the levels of MCP-1,IL-6,IL-1β(except WSTDR group),and TNF-αin spinal cord tissues were significantly reduced in DFO group,WSTDR group,and WSTDR+DFO group(P<0.05,P<0.01).3D laser confocal results showed MECs phagocytosis and clearance of myelin debris were promoted in DFO group and WSTDR group.Conclusion WSTDR promotes MECs to engulf myelin debris by reducing iron overload,improving the infiammatory microenvironment,and promoting spinal cord injury repairment.展开更多
文摘Objective:To observe the intervention effect of clinical nursing pathway on patients receiving intravenous therapy via peripheral mid-long catheters.Methods:Ninety patients receiving intravenous therapy via mid-long catheters in the hospital from March 2024 to March 2025 were selected and randomly divided into a control group and an observation group,with 45 patients in each group.The control group received routine nursing intervention,while the observation group received clinical nursing pathway intervention.The nursing risk events,catheter indwelling time,and nursing satisfaction were evaluated in both groups.Results:The observation group had a shorter catheter indwelling time,fewer risk events during intravenous therapy(total incidence rate of 2.22%),and higher nursing satisfaction.Additionally,negative emotional feedback was reduced,and the SAS score(31.02±1.12)was lower in the observation group compared to the control group.There were significant differences between the two groups in all indicators(P<0.05).Conclusion:Applying the clinical nursing pathway during intravenous therapy via peripheral mid-long catheters can help ensure infusion safety,improve unstable emotional states,and promote high-quality service standards.
基金Supported by the National Natural Science Foundation of China(No.U23A20507 and No.82074522)FU Wen-bin Famous Guangdong TCM Physician Inheritance Studio of Guangdong Provincial TCM Administration(Guangdong TCM Office Letter[2020]No.1)+1 种基金Guangdong Provincial Hospital of Situ Ling lnheritance Studio of Lingnan Acupuncture School(TCM Second Hospital[2013]No.233)Natural Science Foundation of Guangdong Province(No.2024A1515010291)。
文摘Objective To investigate the effects of acupuncture on the neurotransmitter levels and gut microbiota in a mouse model of Tourette syndrome(TS).Methods Thirty-six male C57/BL6 mice were randomly divided into 4 groups using a random number table method:3,3’-iminodipropionitrile(IDPN)group,control group,acupuncture group,and tiapride group,with 9 mice in each group.In the IDPN group,acupuncture group,and tiapride group,mice received daily intraperitoneal injections of IDPN(300 mg/kg body weight)for 7 consecutive days to induce stereotyped behaviors.Subsequently,in the acupuncture intervention group,standardized acupuncture treatment was administered for 14 consecutive days to IDPN-induced TS model mice.The selected acupoints included Baihui(DU 20),Yintang(DU 29),Waiguan(SJ 5),and Zulinqi(GB 41).In the tiapride group,mice were administered tiapride(50 mg/kg body weight)via oral gavage daily for 14 consecutive days.The control group,IDPN group,and acupuncture group received the same volume of saline orally for 14 consecutive days.Stereotypic behaviors were quantified through behavioral assessments.Neurotransmitter levels,including dopamine(DA),glutamate(Glu),and aspartate(ASP)in striatal tissue were measured using enzyme-linked immunosorbent assay.Dopamine transporter(DAT)expression levels were additionally quantified through quantitative polymerase chain reaction(qPCR).Gut microbial composition was analyzed through 16S ribosomal RNA gene sequencing,while metabolic profiling was conducted using liquid chromatography-mass spectrometry(LC-MS).
文摘Objective To study the action mechanism of Wenshen Tongdu Recipe(WSTDR)inpromoting microvascular endothelial cells(MECs)phagocytosis and clearing myelin debris by reducing iron overload phenomenon after spinal cord injury,thereby improving the infiammatory microenvironment after spinal cord injury.Methods Fifty C57BL/6 mice were divided into 5 groups by random number table method:sham operation group,model group,deferoxamine(DFO)group,WSTDR group,WSTDR+DFO group,10 mice in each group.A mouse model of spinal cord injury was prepared by modified Allen's method.The pathological changes of the spinal cord were observed by HE staining and Nissl staining.The iron ion content in serum was determined by the iron ion colorimetric assay kit.The expression levels of monocyte chemoattractant protein-1(MCP-1),interleukin(IL)-6,IL-1β,and tumor necrosis factor-α(TNF-α)in the homogenate and serum of the spinal cord were detected by ELISA.The phagocytosis and clearance of myelin debris by MECs in the spinal cord were observed by 3D laser confocal.Results One mouse died in the model group,DFO group,and WSTDR group,respectively.The results of HE staining showed that compared with the model group,spinal cord cavities were reduced with nerve fiber disorder in DFO group and WSTDR group.Nissl staining analysis showed that compared with the model group,Nissl morphology and neuronal pyknosis were improved in DFO group and WSTDR group.Ferric ion colorimetric assay showed that compared with the model group,serum iron ion concentration were significantly reduced in DFO group and WSTDR group(P<0.01).ELISA results showed that compared with the sham-operation group,the levels of MCP-1,IL-6,IL-1β,and TNF-αin serum and spinal cord tissues significantly increased in the model group(P<0.01).Compared with the model group,serum levels of MCP-1,IL-6,IL-1β,and TNF-αwere significantly lowered in DFO group and WSTDR group(P<0.05,P<0.01).Serum concentrations of MCP-1 and IL-1βwere significantly lowered in WSTDR+DFO group(P<0.01).Compared with WSTDR group,serum concentrations of MCP-1,IL-6,anddIL-1βwere significantly improved in WSTDR+DFO group(P<0.01).Compared with the model group,the levels of MCP-1,IL-6,IL-1β(except WSTDR group),and TNF-αin spinal cord tissues were significantly reduced in DFO group,WSTDR group,and WSTDR+DFO group(P<0.05,P<0.01).3D laser confocal results showed MECs phagocytosis and clearance of myelin debris were promoted in DFO group and WSTDR group.Conclusion WSTDR promotes MECs to engulf myelin debris by reducing iron overload,improving the infiammatory microenvironment,and promoting spinal cord injury repairment.
