As an effective and universal acaricide, amitraz is widely used on beehives against varroasis caused by the mite Varroa jacobsoni. Its residues in honey pose a great danger to human health. In this study, a sensitive,...As an effective and universal acaricide, amitraz is widely used on beehives against varroasis caused by the mite Varroa jacobsoni. Its residues in honey pose a great danger to human health. In this study, a sensitive, rapid, and environmentally friendly surface-enhanced Raman spectroscopy method (SERS) was developed for the determination of trace amount of amitraz in honey with the use of silver nanorod (AgNR) array substrate. The AgNR array substrate fabricated by an oblique angle deposition technique exhibited an excellent SERS activity with an enhancement factor of -10^7. Density function theory was employed to assign the characteristic peak of amitraz. The detection of amitraz was further explored and amitraz in honey at concentrations as low as 0.08 mg/kg can be identified. Specifically, partial least square regression analysis was employed to correlate the SERS spectra in full-wavelength with Camitraz to afford a multiple-quantitative amitraz predicting model. Preliminary results show that the predicted concentrations of amitraz in honey samples are in good agreement with their real concentrations. Compared with the conventional univariate quantitative model based on single peak’s intensity, the proposed multiple-quantitative predicting model integrates all the characteristic peaks of amitraz, thus offering an improved detecting accuracy and anti-interference ability.展开更多
Human serum albumin(HSA)injectable product is a severely afflicted area on drug safety due to its high price and restricted supply.Raman spectroscopy performances high specificity on HSA detection and it is even possi...Human serum albumin(HSA)injectable product is a severely afflicted area on drug safety due to its high price and restricted supply.Raman spectroscopy performances high specificity on HSA detection and it is even possible to determine HSA injectable products noninvasively.In this study,we developed a noninvasive rapid screening method for of HSA injectable products by using portable Raman spectrometer.Qualitative models were established by using principal component analysis combined with classical least squares(PCA-CLS)algorithm,while quanti-tative model was established by using partial least squares(PLS)algorithm.Model transfer in different instruments of both the same and different apparatus modules was further discussed in this paper.A total of 34 HSA injectable samples collected from markets were used for verification.The identification results showed 100%accuracy and the predicted concentrations of those identified as true HSA were consistent with their labeled concentrations.The quantitative results also indicated that model transfer was excellent in the same apparatus modules of Raman spectrometer at all concentration levels,and still good enough in the different apparatus modules although the relative standard deviation(RSD)value showed a little increasing trend at low HSA concentration level.In conclusion,the method was proved to be feasible and efficient for screening HSA injections,especially on its screening speed and the consideration of glass containers.Moreover,with inspiring results on the model transfer,the method could be used as a universal screening mean to different Raman instruments.展开更多
Single crystals of Nd：La3Ga5SiO14 （NLGS） and Nd：La3Ga5.5Ta0.5O14 （NLGT） were grown by Czochralski method. Both crystals exhibit a langasite structure （space group D32 （P321） with Z = 1）, whose general formul...Single crystals of Nd：La3Ga5SiO14 （NLGS） and Nd：La3Ga5.5Ta0.5O14 （NLGT） were grown by Czochralski method. Both crystals exhibit a langasite structure （space group D32 （P321） with Z = 1）, whose general formula is A3BC2D3O14, where A, B, C and D represent the dodecahedral, octahedral and tetrahedral positions, respectively. The adding of low content of Nd does not change the crystal structure. Although they are isostructural, the distribution of atoms among the symmetric sites is different. While NLGT exhibits a perfect orderly structure, NLGS exhibits an intrinsically disordered structure due to the random distribution of Ga and Si atoms at the C3（2） symmetry sites. To understand what changes occur in the phonon distribution when Ta replaces Si, we recorded and analyzed room temperature polarized Raman spectra. We observe 8A1＋18E modes for NLGS and 10A1＋13E for NLGT crystals. When Si is substituted by Ta, we observe that the wavenumber of some modes related to vibrations involving Ga and O atoms decreases. This agrees with the fact that the Ga–O bonds are shorter in NLGS than in NLGT.展开更多
基金supported by the Natural Science Foundation of the Higher Education Institutions of Jiangsu Province (No.16KJB510009 and No.17KJB510017)Jiangsu Province Natural Science Foundation of China (BK20150228)
文摘As an effective and universal acaricide, amitraz is widely used on beehives against varroasis caused by the mite Varroa jacobsoni. Its residues in honey pose a great danger to human health. In this study, a sensitive, rapid, and environmentally friendly surface-enhanced Raman spectroscopy method (SERS) was developed for the determination of trace amount of amitraz in honey with the use of silver nanorod (AgNR) array substrate. The AgNR array substrate fabricated by an oblique angle deposition technique exhibited an excellent SERS activity with an enhancement factor of -10^7. Density function theory was employed to assign the characteristic peak of amitraz. The detection of amitraz was further explored and amitraz in honey at concentrations as low as 0.08 mg/kg can be identified. Specifically, partial least square regression analysis was employed to correlate the SERS spectra in full-wavelength with Camitraz to afford a multiple-quantitative amitraz predicting model. Preliminary results show that the predicted concentrations of amitraz in honey samples are in good agreement with their real concentrations. Compared with the conventional univariate quantitative model based on single peak’s intensity, the proposed multiple-quantitative predicting model integrates all the characteristic peaks of amitraz, thus offering an improved detecting accuracy and anti-interference ability.
基金Youth Develop-ment Research Foundation(No.2015C03)of Na-tional Institutes of Food and Drug Control,P.R.China.
文摘Human serum albumin(HSA)injectable product is a severely afflicted area on drug safety due to its high price and restricted supply.Raman spectroscopy performances high specificity on HSA detection and it is even possible to determine HSA injectable products noninvasively.In this study,we developed a noninvasive rapid screening method for of HSA injectable products by using portable Raman spectrometer.Qualitative models were established by using principal component analysis combined with classical least squares(PCA-CLS)algorithm,while quanti-tative model was established by using partial least squares(PLS)algorithm.Model transfer in different instruments of both the same and different apparatus modules was further discussed in this paper.A total of 34 HSA injectable samples collected from markets were used for verification.The identification results showed 100%accuracy and the predicted concentrations of those identified as true HSA were consistent with their labeled concentrations.The quantitative results also indicated that model transfer was excellent in the same apparatus modules of Raman spectrometer at all concentration levels,and still good enough in the different apparatus modules although the relative standard deviation(RSD)value showed a little increasing trend at low HSA concentration level.In conclusion,the method was proved to be feasible and efficient for screening HSA injections,especially on its screening speed and the consideration of glass containers.Moreover,with inspiring results on the model transfer,the method could be used as a universal screening mean to different Raman instruments.
基金Supported by the Natural Science Foundation of Shandong Province(BS2015CL012,ZR2014JL029)the Higher Education and Technology Development Plan of Shandong Province(J15LA09,J14LA5)Weifang Science and Technology Development Plan(2015GX003)
文摘Single crystals of Nd：La3Ga5SiO14 （NLGS） and Nd：La3Ga5.5Ta0.5O14 （NLGT） were grown by Czochralski method. Both crystals exhibit a langasite structure （space group D32 （P321） with Z = 1）, whose general formula is A3BC2D3O14, where A, B, C and D represent the dodecahedral, octahedral and tetrahedral positions, respectively. The adding of low content of Nd does not change the crystal structure. Although they are isostructural, the distribution of atoms among the symmetric sites is different. While NLGT exhibits a perfect orderly structure, NLGS exhibits an intrinsically disordered structure due to the random distribution of Ga and Si atoms at the C3（2） symmetry sites. To understand what changes occur in the phonon distribution when Ta replaces Si, we recorded and analyzed room temperature polarized Raman spectra. We observe 8A1＋18E modes for NLGS and 10A1＋13E for NLGT crystals. When Si is substituted by Ta, we observe that the wavenumber of some modes related to vibrations involving Ga and O atoms decreases. This agrees with the fact that the Ga–O bonds are shorter in NLGS than in NLGT.
