Rice ragged stunt virus（RRSV） is a serious rice disease in Asia, causing serious yield losses on rice. The capsid protein（CP） gene of the major outer capsid protein of RRSV was expressed in Escherichia coli BL21（...Rice ragged stunt virus（RRSV） is a serious rice disease in Asia, causing serious yield losses on rice. The capsid protein（CP） gene of the major outer capsid protein of RRSV was expressed in Escherichia coli BL21（DE3） using the pMAL-C2 X expression vector. The recombinant protein was used as the immunogen to immunize BALB/c mice. A hybridoma cell line 8A12 secreting monoclonal antibody（MAb） against RRSV was obtained by fusing mouse myeloma cells（Sp 2/0） with spleen cells from the immunized BALB/c mice. Western blot analysis showed that the MAb 8A12 can specifically react with RRSV CP. Using the MAb, an antigen-coated-plate enzyme-linked immunosorbent assay（ACP-ELISA）, a dot enzyme-linked immunosorbent assay（dot-ELISA）, and immunocapture-RT-PCR（IC-RT-PCR） assay were developed to detect RRSV. The established ACP-ELISA, dot-blot ELISA and IC-RT-PCR methods could detect RRSV in infected rice tissue crude extracts with dilutions of 1：40 960, 1：1 280 and 1：655 360（w/v, g mL-1）, respectively. The ACP-ELISA and dot-blot ELISA methods could detect RRSV in infected insect vector crude extracts with dilutions of 1：12 800 and 1：1 600（an individual planthopper μL-1）, respectively. The field survey revealed that Rice ragged stunt disease occurs on rice in Hainan, Yunnan, Guangxi, Sichuan, Guizhou, Fujian, Hunan, Jiangxi and Zhejiang in China.展开更多
大数据技术在地球科学中的应用已成为国家战略重点,《“十四五”大数据产业发展规划》等政策为多源异构数据整合提供了支撑。大语言模型(LLM)是一种基于深度学习技术的人工智能模型。然而,通用大语言模型(如GPT-4、Deepseek-R1)在专业...大数据技术在地球科学中的应用已成为国家战略重点,《“十四五”大数据产业发展规划》等政策为多源异构数据整合提供了支撑。大语言模型(LLM)是一种基于深度学习技术的人工智能模型。然而,通用大语言模型(如GPT-4、Deepseek-R1)在专业领域存在局限性,因训练语料缺乏地球科学细分知识,导致回答笼统或错误(即“幻觉”)(Singhal et al.,2025),Zhou Zhi等(2024)在医疗领域通过Med-PaLM2构建垂直知识库,He Yong等(2024)法律领域基于LawGPT实现法规精准检索。在地球科学领域尚未形成系统的本地化知识问答方案。展开更多
基金supported by the National Basic Research Program of China(2010CB126203)the special fund for Agro-scientific Research in the Public Interest,China(201003031)+1 种基金Earmarked Funds for Modern Agro-industry Technology Research SystemZhejiang Provincial Natural Science Foundation of China(Z3090039)
文摘Rice ragged stunt virus（RRSV） is a serious rice disease in Asia, causing serious yield losses on rice. The capsid protein（CP） gene of the major outer capsid protein of RRSV was expressed in Escherichia coli BL21（DE3） using the pMAL-C2 X expression vector. The recombinant protein was used as the immunogen to immunize BALB/c mice. A hybridoma cell line 8A12 secreting monoclonal antibody（MAb） against RRSV was obtained by fusing mouse myeloma cells（Sp 2/0） with spleen cells from the immunized BALB/c mice. Western blot analysis showed that the MAb 8A12 can specifically react with RRSV CP. Using the MAb, an antigen-coated-plate enzyme-linked immunosorbent assay（ACP-ELISA）, a dot enzyme-linked immunosorbent assay（dot-ELISA）, and immunocapture-RT-PCR（IC-RT-PCR） assay were developed to detect RRSV. The established ACP-ELISA, dot-blot ELISA and IC-RT-PCR methods could detect RRSV in infected rice tissue crude extracts with dilutions of 1：40 960, 1：1 280 and 1：655 360（w/v, g mL-1）, respectively. The ACP-ELISA and dot-blot ELISA methods could detect RRSV in infected insect vector crude extracts with dilutions of 1：12 800 and 1：1 600（an individual planthopper μL-1）, respectively. The field survey revealed that Rice ragged stunt disease occurs on rice in Hainan, Yunnan, Guangxi, Sichuan, Guizhou, Fujian, Hunan, Jiangxi and Zhejiang in China.
文摘大数据技术在地球科学中的应用已成为国家战略重点,《“十四五”大数据产业发展规划》等政策为多源异构数据整合提供了支撑。大语言模型(LLM)是一种基于深度学习技术的人工智能模型。然而,通用大语言模型(如GPT-4、Deepseek-R1)在专业领域存在局限性,因训练语料缺乏地球科学细分知识,导致回答笼统或错误(即“幻觉”)(Singhal et al.,2025),Zhou Zhi等(2024)在医疗领域通过Med-PaLM2构建垂直知识库,He Yong等(2024)法律领域基于LawGPT实现法规精准检索。在地球科学领域尚未形成系统的本地化知识问答方案。
