The formation of·CCl3 radicals in liver nuclei was suggested by spin trapping of them with N-t-butyl-α-phenylnitrone followed by GC/MS detection of the resulting adduct. Comparison of its formation in microsomal...The formation of·CCl3 radicals in liver nuclei was suggested by spin trapping of them with N-t-butyl-α-phenylnitrone followed by GC/MS detection of the resulting adduct. Comparison of its formation in microsomal biotransformation of CCl4 was made. In aerobic nuclear activation mixtures containing NADPH and CCl4, significant decrease in the arachidonic acid content of nuclear lipids was observed (27. 8%, compared to control), the intensity of this decrease was lower than that occurring in the corresponding microsomal incubation mixtures (29.1%). Significant decreases in arachidonic acid content of nuclear and endoplasmic reticulum lipids were also observed in animals at 6 hours of poisoning with the haloalkane. During aerobic nuclear metabolism of CCl4 or CBrCl3, cholesterol oxidation products were detected: a ketocholesterol, an epoxide like structure and 7-ketocholesterol. Nuclear protein carbonyl formation was not promoted during nuclear CCl4 biotransformation. NADPH by itself may lead to protein carbonyl formation during prolonged periods of incubation. CBrCl3 in contrast, led to decreased protein carbonyl formation. No increase in nuclear protein carbonyl formation was observed in CCl4 intoxicated animals during periods of time between 1 to 6 hours after treatment. The results indicate that during nuclear biotransformation of CCl4 or CBrCl3 reactive free radicals, PUFA degradation, reactive aldehydes and cholesterol oxidation products are formed, nearby DNA and regulatory proteins.展开更多
目的:比较完全腹腔镜远端胃癌根治术与腹腔镜辅助远端胃癌根治术对胃癌患者术后恢复情况、疼痛应激反应及外周血T细胞亚群变化的影响。方法:回顾性选取2023年3月—2024年3月在句容市人民医院行远端胃癌根治术治疗的97例患者。根据治疗...目的:比较完全腹腔镜远端胃癌根治术与腹腔镜辅助远端胃癌根治术对胃癌患者术后恢复情况、疼痛应激反应及外周血T细胞亚群变化的影响。方法:回顾性选取2023年3月—2024年3月在句容市人民医院行远端胃癌根治术治疗的97例患者。根据治疗方式不同分为腹腔镜辅助组(49例)和完全腹腔镜组(48例)。观察两组的术后恢复指标、前列腺素E2(PGE2)、β-内啡肽、P物质、CD3^(+)、CD4^(+)、CD8^(+)水平及并发症发生情况。结果:完全腹腔镜组术后下床时间、术后排便时间早于腹腔镜辅助组,术后引流管留置时间、住院时间均短于腹腔镜辅助组(P<0.05)。与术前比较,两组术后1 d血清PGE2、β-内啡肽、P物质水平均升高,但完全腹腔镜组上述指标均较腹腔镜辅助组低(P<0.05)。与术前比较,两组术后1 d CD3^(+)、CD4^(+)均降低,CD8^(+)升高,但完全腹腔镜组CD3^(+)、CD4^(+)较腹腔镜辅助组高,CD8^(+)较腹腔镜辅助组低(P<0.05)。完全腹腔镜组的术后并发症总发生率低于腹腔镜辅助组(P<0.05)。结论:相较于腹腔镜辅助远端胃癌根治术,完全腹腔镜远端胃癌根治术能加速患者恢复,减轻疼痛应激,并优化外周血T细胞亚群比例,安全性更高。展开更多
目的:了解国内外全膀胱切除病人衰弱的研究现况,为开展衰弱的精准干预提供参考。方法:计算机系统检索PubMed、Embase、Web of Science、CINAHL、the Cochrane Library、中国知网、中国生物医学文献数据库、维普数据库、万方数据库中有...目的:了解国内外全膀胱切除病人衰弱的研究现况,为开展衰弱的精准干预提供参考。方法:计算机系统检索PubMed、Embase、Web of Science、CINAHL、the Cochrane Library、中国知网、中国生物医学文献数据库、维普数据库、万方数据库中有关全膀胱切除病人衰弱的文献,检索时限为建库至2024年9月28日。结果:共纳入25篇文献,其中回顾性分析19篇(其中回顾性队列研究4篇,回顾性病例对照研究1篇)、前瞻性分析4篇、随机对照试验(RCT)1篇、前瞻性+回顾性研究1篇。结论:全膀胱切除病人衰弱的研究主要集中于衰弱的评估及其与各类结局指标的关系研究,针对衰弱的干预方案较少,且衰弱的测量工具多样化尚未达成共识。提出未来强化全膀胱切除术前常态化、标准化衰弱评估,构建全膀胱切除病人术前风险分层方案,实施以专科护士为主导的预康复干预方案,以减少老龄化背景下术后多项不良结局发生的风险,最大程度助力病人安全度过围术期。展开更多
文摘The formation of·CCl3 radicals in liver nuclei was suggested by spin trapping of them with N-t-butyl-α-phenylnitrone followed by GC/MS detection of the resulting adduct. Comparison of its formation in microsomal biotransformation of CCl4 was made. In aerobic nuclear activation mixtures containing NADPH and CCl4, significant decrease in the arachidonic acid content of nuclear lipids was observed (27. 8%, compared to control), the intensity of this decrease was lower than that occurring in the corresponding microsomal incubation mixtures (29.1%). Significant decreases in arachidonic acid content of nuclear and endoplasmic reticulum lipids were also observed in animals at 6 hours of poisoning with the haloalkane. During aerobic nuclear metabolism of CCl4 or CBrCl3, cholesterol oxidation products were detected: a ketocholesterol, an epoxide like structure and 7-ketocholesterol. Nuclear protein carbonyl formation was not promoted during nuclear CCl4 biotransformation. NADPH by itself may lead to protein carbonyl formation during prolonged periods of incubation. CBrCl3 in contrast, led to decreased protein carbonyl formation. No increase in nuclear protein carbonyl formation was observed in CCl4 intoxicated animals during periods of time between 1 to 6 hours after treatment. The results indicate that during nuclear biotransformation of CCl4 or CBrCl3 reactive free radicals, PUFA degradation, reactive aldehydes and cholesterol oxidation products are formed, nearby DNA and regulatory proteins.
文摘目的:比较完全腹腔镜远端胃癌根治术与腹腔镜辅助远端胃癌根治术对胃癌患者术后恢复情况、疼痛应激反应及外周血T细胞亚群变化的影响。方法:回顾性选取2023年3月—2024年3月在句容市人民医院行远端胃癌根治术治疗的97例患者。根据治疗方式不同分为腹腔镜辅助组(49例)和完全腹腔镜组(48例)。观察两组的术后恢复指标、前列腺素E2(PGE2)、β-内啡肽、P物质、CD3^(+)、CD4^(+)、CD8^(+)水平及并发症发生情况。结果:完全腹腔镜组术后下床时间、术后排便时间早于腹腔镜辅助组,术后引流管留置时间、住院时间均短于腹腔镜辅助组(P<0.05)。与术前比较,两组术后1 d血清PGE2、β-内啡肽、P物质水平均升高,但完全腹腔镜组上述指标均较腹腔镜辅助组低(P<0.05)。与术前比较,两组术后1 d CD3^(+)、CD4^(+)均降低,CD8^(+)升高,但完全腹腔镜组CD3^(+)、CD4^(+)较腹腔镜辅助组高,CD8^(+)较腹腔镜辅助组低(P<0.05)。完全腹腔镜组的术后并发症总发生率低于腹腔镜辅助组(P<0.05)。结论:相较于腹腔镜辅助远端胃癌根治术,完全腹腔镜远端胃癌根治术能加速患者恢复,减轻疼痛应激,并优化外周血T细胞亚群比例,安全性更高。
文摘目的:了解国内外全膀胱切除病人衰弱的研究现况,为开展衰弱的精准干预提供参考。方法:计算机系统检索PubMed、Embase、Web of Science、CINAHL、the Cochrane Library、中国知网、中国生物医学文献数据库、维普数据库、万方数据库中有关全膀胱切除病人衰弱的文献,检索时限为建库至2024年9月28日。结果:共纳入25篇文献,其中回顾性分析19篇(其中回顾性队列研究4篇,回顾性病例对照研究1篇)、前瞻性分析4篇、随机对照试验(RCT)1篇、前瞻性+回顾性研究1篇。结论:全膀胱切除病人衰弱的研究主要集中于衰弱的评估及其与各类结局指标的关系研究,针对衰弱的干预方案较少,且衰弱的测量工具多样化尚未达成共识。提出未来强化全膀胱切除术前常态化、标准化衰弱评估,构建全膀胱切除病人术前风险分层方案,实施以专科护士为主导的预康复干预方案,以减少老龄化背景下术后多项不良结局发生的风险,最大程度助力病人安全度过围术期。
