Objective:The present study investigated the cytoprotective effects of a Pogonatherum paniceum extract prepared with 80%ethanol(PPE)using synchrotron radiation-based Fourier transform infrared(SR-FTIR)microspectroscop...Objective:The present study investigated the cytoprotective effects of a Pogonatherum paniceum extract prepared with 80%ethanol(PPE)using synchrotron radiation-based Fourier transform infrared(SR-FTIR)microspectroscopy and determined its phytochemical profile.Methods:The volatile and polyphenolic compounds in PPE were characterized using gas chromatography–mass spectrometry and liquid chromatography–mass spectrometry,respectively.The antioxidant capacity of PPE was evaluated using chemical and cell-based assays.The SR-FTIR microspectroscopy was performed to evaluate the cytoprotective effect of PPE by identifying changes in macromolecule composition in tert-butyl hydroperoxide(t BuOOH)-induced oxidative damage in RAW264.7 cells.Results:A total of 48 volatile compounds and 28 polyphenol components were found in PPE.PPE exhibited a high potential for antioxidant activity by scavenging the intracellular reactive oxygen species in t Bu OOH-induced oxidative damage in RAW264.7 cells.PPE treatment also significantly protected RAW264.7 cells against t BuOOH-induced toxicity and restored cell viability.The SR-FTIR analysis revealed that t BuOOH increased the lipid and ester lipid content in RAW264.7 cells.The PPE exerted a cytoprotective effect by decreasing the levels of lipid and ester lipid compounds that had been elevated by t BuOOH in RAW264.7 cells.These findings indicate that PPE has cytoprotective potential due to its ability to inhibit endogenous reactive oxygen species.Conclusion:This study extends the current knowledge on the phytochemistry of PPE and its antioxidant and cytoprotective effects.These findings support the use of SR-FTIR microspectroscopy to determine the cytoprotective effects of natural products.PPE extract may be a candidate compound for new therapeutics and nutraceuticals that target the prevention of oxidative stress-associated diseases.展开更多
Background Accurate estimation of potential evapotranspiration(PET)is the key for studying land-air interaction hydrological processes.Several models are used to estimate the PET based on standardized meteorological d...Background Accurate estimation of potential evapotranspiration(PET)is the key for studying land-air interaction hydrological processes.Several models are used to estimate the PET based on standardized meteorological data.Although combination-based models have the highest level performance estimation of PET,they require more meteorological data and may therefore be difficult to apply in areas lacking meteorological observation data.Results The results showed significant differences in the spatial trends of PET calculated by different models in China,the Doorenbots–Pruitts model revealed the highest PET(1902.6 mm),and the Kuzmin model revealed the lowest PET(349.6 mm),with the largest difference being 5.5 times.The Romanenko and the Rohwer models were the recommended temperature-based and aerodynamic-based models.On the other hand,the Abtew model was more suitable for arid and semi-arid regions,while the Priestley–Taylor model was more suitable for humid regions.Combination-based models revealed ideal calculation accuracies,among which the Penman–Monteith model was the best option for PET calculation.Conclusions The accuracy range of Romanenko,Rohwer,Abten,Priestley Taylor,and Penman Monteith models improved in MPZ and TCZ is higher than that improved in TMZ and SMZ.This does not mean that the improved models have higher accuracy in MPZ and TCZ than in TMZ and SMZ.On the contrary,the original model performed poorly in MPZ and TCZ,so the improved accuracy was relatively large.The unimproved model was already more suitable in TMZ and SMZ,so the improved accuracy was relatively small.Therefore,regional calibration of the PET models can improve the accuracy and applicability of PET calculation,providing a reference for studying hydrological processes in different climatic zones.展开更多
基金supported by the Basic Research Fund from Thailand Science Research and Innovation through Sisaket Rajabhat University(grant number:FF.13/2564)。
文摘Objective:The present study investigated the cytoprotective effects of a Pogonatherum paniceum extract prepared with 80%ethanol(PPE)using synchrotron radiation-based Fourier transform infrared(SR-FTIR)microspectroscopy and determined its phytochemical profile.Methods:The volatile and polyphenolic compounds in PPE were characterized using gas chromatography–mass spectrometry and liquid chromatography–mass spectrometry,respectively.The antioxidant capacity of PPE was evaluated using chemical and cell-based assays.The SR-FTIR microspectroscopy was performed to evaluate the cytoprotective effect of PPE by identifying changes in macromolecule composition in tert-butyl hydroperoxide(t BuOOH)-induced oxidative damage in RAW264.7 cells.Results:A total of 48 volatile compounds and 28 polyphenol components were found in PPE.PPE exhibited a high potential for antioxidant activity by scavenging the intracellular reactive oxygen species in t Bu OOH-induced oxidative damage in RAW264.7 cells.PPE treatment also significantly protected RAW264.7 cells against t BuOOH-induced toxicity and restored cell viability.The SR-FTIR analysis revealed that t BuOOH increased the lipid and ester lipid content in RAW264.7 cells.The PPE exerted a cytoprotective effect by decreasing the levels of lipid and ester lipid compounds that had been elevated by t BuOOH in RAW264.7 cells.These findings indicate that PPE has cytoprotective potential due to its ability to inhibit endogenous reactive oxygen species.Conclusion:This study extends the current knowledge on the phytochemistry of PPE and its antioxidant and cytoprotective effects.These findings support the use of SR-FTIR microspectroscopy to determine the cytoprotective effects of natural products.PPE extract may be a candidate compound for new therapeutics and nutraceuticals that target the prevention of oxidative stress-associated diseases.
基金National Natural Science Foundation of China[U2243202],[42230714,41977149]
文摘Background Accurate estimation of potential evapotranspiration(PET)is the key for studying land-air interaction hydrological processes.Several models are used to estimate the PET based on standardized meteorological data.Although combination-based models have the highest level performance estimation of PET,they require more meteorological data and may therefore be difficult to apply in areas lacking meteorological observation data.Results The results showed significant differences in the spatial trends of PET calculated by different models in China,the Doorenbots–Pruitts model revealed the highest PET(1902.6 mm),and the Kuzmin model revealed the lowest PET(349.6 mm),with the largest difference being 5.5 times.The Romanenko and the Rohwer models were the recommended temperature-based and aerodynamic-based models.On the other hand,the Abtew model was more suitable for arid and semi-arid regions,while the Priestley–Taylor model was more suitable for humid regions.Combination-based models revealed ideal calculation accuracies,among which the Penman–Monteith model was the best option for PET calculation.Conclusions The accuracy range of Romanenko,Rohwer,Abten,Priestley Taylor,and Penman Monteith models improved in MPZ and TCZ is higher than that improved in TMZ and SMZ.This does not mean that the improved models have higher accuracy in MPZ and TCZ than in TMZ and SMZ.On the contrary,the original model performed poorly in MPZ and TCZ,so the improved accuracy was relatively large.The unimproved model was already more suitable in TMZ and SMZ,so the improved accuracy was relatively small.Therefore,regional calibration of the PET models can improve the accuracy and applicability of PET calculation,providing a reference for studying hydrological processes in different climatic zones.
