The effect of pre-irradiation on radiation sensitivity of fiber Bragg gratings （FBGs） is verified experimentally. FBGs are fabricated in photosensitive optical fibers and single mode fibers with Ce-concentration in ...The effect of pre-irradiation on radiation sensitivity of fiber Bragg gratings （FBGs） is verified experimentally. FBGs are fabricated in photosensitive optical fibers and single mode fibers with Ce-concentration in a range from 3 mol% to 23.37 mol% in the core. In experiments, the FBGs are subjected to twice v-radiation exposures to a Co^60 source at a dose-rate of 0.1 Gy/s up to a total dose of 50 kGy. Pre-irradiation treatment can reduce the temperature sensitivity variation of FBGs by 18.12%-35.91%, as well as Bragg wavelength shift （BWS） by 8%-27.08 %. Our research demonstrates that pre-irradiation treatment is a feasible method to improve the radiation tolerance of FBGs.展开更多
To overcome the limitations of traditional single-crystal X-ray diffraction(SCXRD)for microcrystalline materials and the peak-overlapping issue of powder X-ray diffraction(PXRD),this study employed cryogenic continuou...To overcome the limitations of traditional single-crystal X-ray diffraction(SCXRD)for microcrystalline materials and the peak-overlapping issue of powder X-ray diffraction(PXRD),this study employed cryogenic continuous rotation electron diffraction(cryo-cRED)with a low-dose strategy to determine the crystal structure of CL30,a novel silicogermanate framework.It is confirmed that CL30 crystallizes in the C2/m space group and has layered topology composed of discontinuous zigzag chains connected by double four-membered ring(d4r)units,with fluoride anions(F^(-))occluded in the d4r units.In CL30,charge balance involves organic structure-directing agent(OSDA)cations,occluded F^(-),and terminal oxygen sites whose protonation state cannot be established from the present three dimensional(3D)ED data.F^(-)encapsulated in the d4r units contributes to charge compensation as the counter-anion to OSDA cations,rather than only balancing the framework charge.Although the refinement indices(R_(1)=0.29,wR_(2)=0.71)exceeded typical small-molecule crystallography standards,the structural model remained highly reliable,as supported by geometric restraints and validation.In electron diffraction,elevated R_(1) values are commonly attributed to the intrinsic factors of the technique,such as dynamic scattering,detector noise from scintillator-based detectors,and TEM stage instability(large spheres of confusion).This study introduces a new structural prototype to the silicogermanate family and establishes a feasible workflow for determining the structures of radiation-sensitive microcrystalline porous materials.展开更多
Summary： The HL-60 cells were transfected with chkl antisense and sense chain, and 24 h later subjected to irradiation. Twenty-four h after irradiation, the changes in the chk1 protein expression was assayed by Weste...Summary： The HL-60 cells were transfected with chkl antisense and sense chain, and 24 h later subjected to irradiation. Twenty-four h after irradiation, the changes in the chk1 protein expression was assayed by Western blot, and the cell cycles and apoptosis rate detected by FCM. The irradiated apoptosis sensitivity was increased by antisense blocking of chk1 gene in HL-60 cell line with the apoptosis rate being 26.31 %, significantly higher than that by the sense blocking （10.34 %, 0. 025〈P〈0.05）. In HL-60 cells transfected with chkl antisense chain, the G2/M phase arrest was attenua：ted and the cells in G2/M phase were accounted for 38.42 %, significantly lower than those of the cells transfected with chkl sense chain （54.64 %, 0. 005〈P〈0.01）. It was concluded that antisense blocking of chk1 gene could increase the apoptosis sensitivity to irradiation.展开更多
The IAP AGCM was used to simulate the climate of 125kyr and 115kyr before present. We analysed the results and then studied the sensitivity of the model to the changes of radiation distribution induced by orbital para...The IAP AGCM was used to simulate the climate of 125kyr and 115kyr before present. We analysed the results and then studied the sensitivity of the model to the changes of radiation distribution induced by orbital parameter changes. The reasonability of the results was also discussed.展开更多
Radiotherapy is a part of the front-line treatment regime for many cancers. The mechanisms of radiation-induced effects in cancers mainly involves double-strand breaks (DBS) which plays very important role in maintain...Radiotherapy is a part of the front-line treatment regime for many cancers. The mechanisms of radiation-induced effects in cancers mainly involves double-strand breaks (DBS) which plays very important role in maintaining the stability of gene. As DNA repair gene breast cancer 1 (BRCA1) and DNA-dependent protein kinase catalytic subunit (DNA-PKcs) can act to maintain genetic stability though two distinct and complementary mechanisms for DNA DSB repair-homologous recombination (HR) and non-homologous end joining (NHEJ). Therefor, BRCA1 and DNA-PKcs are closely associated with radiation sensitivity, which means that they may be used as a useful tool to predict radio sensitivity in human tumour cells.展开更多
AIM： We studied the effect of colchicine combined with radiation on the survival of human hepatocellular carcinoma （HCC） HA22T/VGH cells.METHODS： Twenty-four hours after treatment with 0-8 ng/mL colchicine, HA22T/...AIM： We studied the effect of colchicine combined with radiation on the survival of human hepatocellular carcinoma （HCC） HA22T/VGH cells.METHODS： Twenty-four hours after treatment with 0-8 ng/mL colchicine, HA22T/VGH cells were irradiated at various doses （0, 1, 2, 4, and 8 Gy）. Colony assay was performed to assess the surviving cell fraction. Survival curves were fitted by using a linear-quadratic model to estimate the sensitizer enhancement ratio （SER）. Flow cytometry was used for cell cycle analysis.RESULTS： Colchicine at lower concentrations （1 and 2 ng/mL） had obvious synergy with radiation to inhibit HCC cell growth, whereas higher concentrations （4 and 8 ng/mL） had only additive effect to radiation. Pretreatment with 1 and 2 ng/mL colchicine for 24-h enhanced cell killing by radiation with SERs of 1.21 and 1.53, respectively.G2/N arrest was only observed with higher colchicine doses （8 and 16 ng/mL） after 24-h treatment, this effect was neither seen with lower doses （1, 2, and 4 ng/mL）nor with any dose after only 1 h of treatment.CONCLUSION： Our results suggest that colchicine has potential as an adjunct to radiotherapy for HCC treatment.Lower doses of colchicine possess radiosensitizing effects via some mechanism other than G2/M arrest. Further study is necessary to elucidate the mechanism.展开更多
Forest canopy reduces shortwave radiation and increases the incoming longwave radiation to snowpacks beneath forest canopies. Furthermore, the effect of forest canopy may be changed by complex topography. In this pape...Forest canopy reduces shortwave radiation and increases the incoming longwave radiation to snowpacks beneath forest canopies. Furthermore, the effect of forest canopy may be changed by complex topography. In this paper, we measured and simulated the incoming longwave radiation to snow beneath forest at different canopy openness in the west Tianshan Mountains, China(43°16'N, 84°24'E) during spring 2013. A sensitivity study was conducted to explore the way that terrain influenced the incoming longwave radiation to snow beneath forest canopies. In the simulation model, measurement datasets, including air temperature, incoming shortwave radiation above canopy, and longwave radiation enhanced by adjacent terrain, were applied to calculate the incoming longwave radiation to snow beneath forest canopy. The simulation results were consistent with the measurements on hourly scale and daily scale. The effect of longwave radiation enhanced by terrain was important than that of shortwave radiation above forest canopy with different openness except the 20% canopy openness. The longwave radiation enhanced due to adjacent terrain increases with the slope increase and temperature rise. When air temperature(or slope) is relatively low, thelongwave radiation enhanced by adjacent terrain is not sensitive to slope(or air temperature), but the sensitivity increases with the decrease of snow cover area on sunny slope. The effect of longwave radiation is especially sensitive when the snow cover on sunny slope melts completely. The effect of incoming shortwave radiation reflected by adjacent terrain on incoming longwave radiation to snow beneath forest canopies is more slight than that of the enhanced longwave radiation.展开更多
Radiotherapy(RT)is a crucial treatment for cancer;however,its effectiveness is limited by adverse effects on normal tissues,radioresistance,and tumor recurrence.To overcome these challenges,hydrogels have been employe...Radiotherapy(RT)is a crucial treatment for cancer;however,its effectiveness is limited by adverse effects on normal tissues,radioresistance,and tumor recurrence.To overcome these challenges,hydrogels have been employed for delivery of radiosensitizers and other therapeutic agents.This review summarizes recent advancements in the application of hydrogel-based local drug delivery systems for improving the therapeutic efficacy of RT in cancer treatment.Firstly,we introduce the classification and properties of hydrogels.Next,we detail hydrogel-based platforms designed to enhance both external beam radiation therapy and brachytherapy.We also discuss hydrogels used in combination therapy involving RT and immunotherapy.Lastly,we highlight the challenges that hydrogels face in RT.By surveying the latest developments in hydrogel applications for RT,this review aims to provide insights into the development of more effective and targeted cancer therapies.展开更多
Background:Radiation sensitive 23 homolog B(RAD23B),a DNA repair-related protein,plays a contributory role in the development of multiple malignancies.This study aimed to explore the role of RAD23B in promoting colore...Background:Radiation sensitive 23 homolog B(RAD23B),a DNA repair-related protein,plays a contributory role in the development of multiple malignancies.This study aimed to explore the role of RAD23B in promoting colorectal cancer(CRC)metastasis and to elucidate the underlying molecular mechanisms.Methods:RAD23B was overexpressed in CRC cell lines SW480 and HCT-8,with empty vectors serving as controls.Invasion,cell proliferation,and migration were assessed using CCK-8 and Transwell assays.A xenograft mouse model was used to evaluate metastatic potential in vivo.Immunoprecipitation-mass spectrometry(IP-MS)and transcriptomic analysis by RNA sequencing(RNA-seq)were performed to identify signaling pathways regulated by RAD23B.Western blotting was used to analyze the expression of RAD23B,Talin1,Integrinsαv/β1,PI3K,p-PI3K,AKT,p-AKT,and MMP9.Immunohistochemistry was conducted to examine RAD23B and Integrinβ1 expression in CRC tissues.Results:RAD23B overexpression notably enhanced CRC migration,cell proliferation,and invasion both in vitro and in vivo.IP-MS,RNA-seq,and protein analysis revealed that RAD23B upregulated Talin1 and Integrinsαv/β1,resulting in an activation of the PI3K/AKT signaling pathway.Moreover,RAD23B promoted MMP9 expression,contributing to enhanced invasive potential.Conclusion:RAD23B facilitates CRCmetastasis through activation of the Talin1/Integrinαv/β1/PI3K/AKT/MMP9 signaling axis.These results provide novel insights into the role of RAD23B in CRC progression and identify it as a potential therapeutic target.展开更多
Metal halide perovskites,particularly lead-based compounds,manifest great potential in X-ray detection due to their exceptional light absorption coefficients,superior carrier mobility and cost-effective preparation me...Metal halide perovskites,particularly lead-based compounds,manifest great potential in X-ray detection due to their exceptional light absorption coefficients,superior carrier mobility and cost-effective preparation methods.Despite recent significant advancements,the limitations of lead toxicity hinder their further application and encourage the exploration of environmentally friendly alternatives.Herein,a novel lead-free two-dimensional(2D)copper-based halide perovskite(2FEA)_(2)CuCl_(4)(2FEA=2,2-difluoroethylamine)single crystal is developed,which exhibits a highly sensitive response to X-ray illumination.In particular,an inch-sized single crystal of(2FEA)_(2)CuCl_(4)with dimensions of up to 30×28×0.5 mm^(3)is grown using a low-cost cooling crystallization method.Direct X-ray detectors fabricated from these single crystals exhibited an exceptionally high sensitivity of 1106.44μC Gy_(air)^(−1)cm^(−2)at 10 V bias,approximately 43 times higher than that of the conventional radiation-sensitive semiconductor α-Se(~25μC Gy_(air)^(−1)cm^(−2)).Furthermore,(2FEA)2CuCl4 exhibits a stable baseline with a low dark current drift of 5.056×10^(−7)nA cm^(−1)s^(−1)V^(−1)and a low limit of detection of 130.1 nGy_(air)s^(−1)at 10 V bias.These findings manifest the great potential of 2D copper-based hybrid perovskites for next-generation highly sensitive X-ray detection,which sheds light on the rational crystal design of“green”radiation-sensitive hybrid perovskites.展开更多
Objective:To investigate the contribution of YWHAZ gene on the radioresistance and metastasis ability of oral squamous cell carcinoma(OSCC)cells.Mathods:The relationship between the expression level of YWHAZ gene and ...Objective:To investigate the contribution of YWHAZ gene on the radioresistance and metastasis ability of oral squamous cell carcinoma(OSCC)cells.Mathods:The relationship between the expression level of YWHAZ gene and the survival of head and neck squamous cell carcinoma(HNSC)patients was analyzed using Gene Expression Profiling Interactive Analysis(GEPIA)database.A radioresistance cell line(CAL-27R)was constructed by irradiating CAL-27 cells with frac-tional doses.Cell survival was measured by colony formation assay.Cell migration and invasion were detected by transwell assay.The formation of yH2AX foci was detected by immunofluorescence assay,The protein expressions were detected by Westemn blot assay.In some experiments,CAL-27R cells were effectively transferred with siRNA YWHAZ(siYWHAZ).Results:GEPIA database showed that the expression level of YWHAZ in HNSC tumors was higher than that in adjacent normal tissues,and the HNSC patients with higher level of YWHAZ had a shorter survival.In vitro experiments demonstrated that the expression of YWHAZ protein in CAL-27 cells was lower than HSC-3 cells(t=18.89,P<0.01)and radioresistant CAL-27R cells(t=25.70,P<0.01).Knockdown of YWHAZ gene significantly increased radiation-induced cell killing effect,apoptosis induction,and yH2AX foci formation inCAL-27R and HSC-3 cells.Moreover,siRNA YWHAZ transfection also reduced the invasion and migration abilities of the irradiated CAL 27R[t=21.09,P<0.01(migration);t=6.16,P<0.05(invasion)]and HSC-3 cells[t=34.53,P<0.001(migration);t=4.92,P<0.05(invasion)]and attenuated radiation-induced expressions of metastasis-related proteins,Conclusion:YWHAZ contributes to the radioresistance of oral squamous cells and thus it may applicable to be a potential target for OSOC radiotherapy.展开更多
基金Project supported by the National Natural Science Foundation of China (Grant No. 61007040)the Fundamental Research Funds for Central Universities
文摘The effect of pre-irradiation on radiation sensitivity of fiber Bragg gratings （FBGs） is verified experimentally. FBGs are fabricated in photosensitive optical fibers and single mode fibers with Ce-concentration in a range from 3 mol% to 23.37 mol% in the core. In experiments, the FBGs are subjected to twice v-radiation exposures to a Co^60 source at a dose-rate of 0.1 Gy/s up to a total dose of 50 kGy. Pre-irradiation treatment can reduce the temperature sensitivity variation of FBGs by 18.12%-35.91%, as well as Bragg wavelength shift （BWS） by 8%-27.08 %. Our research demonstrates that pre-irradiation treatment is a feasible method to improve the radiation tolerance of FBGs.
基金supported by the National Natural Science Foundation of China (Grant No.12374021)Beijing Natural Science Foundation (Grant No.1252031)。
文摘To overcome the limitations of traditional single-crystal X-ray diffraction(SCXRD)for microcrystalline materials and the peak-overlapping issue of powder X-ray diffraction(PXRD),this study employed cryogenic continuous rotation electron diffraction(cryo-cRED)with a low-dose strategy to determine the crystal structure of CL30,a novel silicogermanate framework.It is confirmed that CL30 crystallizes in the C2/m space group and has layered topology composed of discontinuous zigzag chains connected by double four-membered ring(d4r)units,with fluoride anions(F^(-))occluded in the d4r units.In CL30,charge balance involves organic structure-directing agent(OSDA)cations,occluded F^(-),and terminal oxygen sites whose protonation state cannot be established from the present three dimensional(3D)ED data.F^(-)encapsulated in the d4r units contributes to charge compensation as the counter-anion to OSDA cations,rather than only balancing the framework charge.Although the refinement indices(R_(1)=0.29,wR_(2)=0.71)exceeded typical small-molecule crystallography standards,the structural model remained highly reliable,as supported by geometric restraints and validation.In electron diffraction,elevated R_(1) values are commonly attributed to the intrinsic factors of the technique,such as dynamic scattering,detector noise from scintillator-based detectors,and TEM stage instability(large spheres of confusion).This study introduces a new structural prototype to the silicogermanate family and establishes a feasible workflow for determining the structures of radiation-sensitive microcrystalline porous materials.
文摘Summary： The HL-60 cells were transfected with chkl antisense and sense chain, and 24 h later subjected to irradiation. Twenty-four h after irradiation, the changes in the chk1 protein expression was assayed by Western blot, and the cell cycles and apoptosis rate detected by FCM. The irradiated apoptosis sensitivity was increased by antisense blocking of chk1 gene in HL-60 cell line with the apoptosis rate being 26.31 %, significantly higher than that by the sense blocking （10.34 %, 0. 025〈P〈0.05）. In HL-60 cells transfected with chkl antisense chain, the G2/M phase arrest was attenua：ted and the cells in G2/M phase were accounted for 38.42 %, significantly lower than those of the cells transfected with chkl sense chain （54.64 %, 0. 005〈P〈0.01）. It was concluded that antisense blocking of chk1 gene could increase the apoptosis sensitivity to irradiation.
文摘The IAP AGCM was used to simulate the climate of 125kyr and 115kyr before present. We analysed the results and then studied the sensitivity of the model to the changes of radiation distribution induced by orbital parameter changes. The reasonability of the results was also discussed.
文摘Radiotherapy is a part of the front-line treatment regime for many cancers. The mechanisms of radiation-induced effects in cancers mainly involves double-strand breaks (DBS) which plays very important role in maintaining the stability of gene. As DNA repair gene breast cancer 1 (BRCA1) and DNA-dependent protein kinase catalytic subunit (DNA-PKcs) can act to maintain genetic stability though two distinct and complementary mechanisms for DNA DSB repair-homologous recombination (HR) and non-homologous end joining (NHEJ). Therefor, BRCA1 and DNA-PKcs are closely associated with radiation sensitivity, which means that they may be used as a useful tool to predict radio sensitivity in human tumour cells.
基金Supported by the MMH grant from Mackay Memorial Hospital, No. 9252
文摘AIM： We studied the effect of colchicine combined with radiation on the survival of human hepatocellular carcinoma （HCC） HA22T/VGH cells.METHODS： Twenty-four hours after treatment with 0-8 ng/mL colchicine, HA22T/VGH cells were irradiated at various doses （0, 1, 2, 4, and 8 Gy）. Colony assay was performed to assess the surviving cell fraction. Survival curves were fitted by using a linear-quadratic model to estimate the sensitizer enhancement ratio （SER）. Flow cytometry was used for cell cycle analysis.RESULTS： Colchicine at lower concentrations （1 and 2 ng/mL） had obvious synergy with radiation to inhibit HCC cell growth, whereas higher concentrations （4 and 8 ng/mL） had only additive effect to radiation. Pretreatment with 1 and 2 ng/mL colchicine for 24-h enhanced cell killing by radiation with SERs of 1.21 and 1.53, respectively.G2/N arrest was only observed with higher colchicine doses （8 and 16 ng/mL） after 24-h treatment, this effect was neither seen with lower doses （1, 2, and 4 ng/mL）nor with any dose after only 1 h of treatment.CONCLUSION： Our results suggest that colchicine has potential as an adjunct to radiotherapy for HCC treatment.Lower doses of colchicine possess radiosensitizing effects via some mechanism other than G2/M arrest. Further study is necessary to elucidate the mechanism.
基金funded by National Key Technology Research and Development Program of the Ministry of Science and Technology of China(Grant No.2012BAC23B01)National Natural Science Foundation of China(Grant Nos.41271098,41171066)China Special Fund for Meteorological Research in the Public Interest(GYHY201206026)
文摘Forest canopy reduces shortwave radiation and increases the incoming longwave radiation to snowpacks beneath forest canopies. Furthermore, the effect of forest canopy may be changed by complex topography. In this paper, we measured and simulated the incoming longwave radiation to snow beneath forest at different canopy openness in the west Tianshan Mountains, China(43°16'N, 84°24'E) during spring 2013. A sensitivity study was conducted to explore the way that terrain influenced the incoming longwave radiation to snow beneath forest canopies. In the simulation model, measurement datasets, including air temperature, incoming shortwave radiation above canopy, and longwave radiation enhanced by adjacent terrain, were applied to calculate the incoming longwave radiation to snow beneath forest canopy. The simulation results were consistent with the measurements on hourly scale and daily scale. The effect of longwave radiation enhanced by terrain was important than that of shortwave radiation above forest canopy with different openness except the 20% canopy openness. The longwave radiation enhanced due to adjacent terrain increases with the slope increase and temperature rise. When air temperature(or slope) is relatively low, thelongwave radiation enhanced by adjacent terrain is not sensitive to slope(or air temperature), but the sensitivity increases with the decrease of snow cover area on sunny slope. The effect of longwave radiation is especially sensitive when the snow cover on sunny slope melts completely. The effect of incoming shortwave radiation reflected by adjacent terrain on incoming longwave radiation to snow beneath forest canopies is more slight than that of the enhanced longwave radiation.
基金supported by Funds of Sichuan Province for Distinguished Young Scholar(No.2021JDJQ0037)Key Research and Development Program of Sichuan Province(No.2023YFS0153)1·3·5 project for disciplines of excellence,West China Hospital,Sichuan University(No.ZYYC08002).
文摘Radiotherapy(RT)is a crucial treatment for cancer;however,its effectiveness is limited by adverse effects on normal tissues,radioresistance,and tumor recurrence.To overcome these challenges,hydrogels have been employed for delivery of radiosensitizers and other therapeutic agents.This review summarizes recent advancements in the application of hydrogel-based local drug delivery systems for improving the therapeutic efficacy of RT in cancer treatment.Firstly,we introduce the classification and properties of hydrogels.Next,we detail hydrogel-based platforms designed to enhance both external beam radiation therapy and brachytherapy.We also discuss hydrogels used in combination therapy involving RT and immunotherapy.Lastly,we highlight the challenges that hydrogels face in RT.By surveying the latest developments in hydrogel applications for RT,this review aims to provide insights into the development of more effective and targeted cancer therapies.
基金supported by Hebei Natural Science Foundation(No.H2022105001)S&T Program of Hebei(No.22377727D).
文摘Background:Radiation sensitive 23 homolog B(RAD23B),a DNA repair-related protein,plays a contributory role in the development of multiple malignancies.This study aimed to explore the role of RAD23B in promoting colorectal cancer(CRC)metastasis and to elucidate the underlying molecular mechanisms.Methods:RAD23B was overexpressed in CRC cell lines SW480 and HCT-8,with empty vectors serving as controls.Invasion,cell proliferation,and migration were assessed using CCK-8 and Transwell assays.A xenograft mouse model was used to evaluate metastatic potential in vivo.Immunoprecipitation-mass spectrometry(IP-MS)and transcriptomic analysis by RNA sequencing(RNA-seq)were performed to identify signaling pathways regulated by RAD23B.Western blotting was used to analyze the expression of RAD23B,Talin1,Integrinsαv/β1,PI3K,p-PI3K,AKT,p-AKT,and MMP9.Immunohistochemistry was conducted to examine RAD23B and Integrinβ1 expression in CRC tissues.Results:RAD23B overexpression notably enhanced CRC migration,cell proliferation,and invasion both in vitro and in vivo.IP-MS,RNA-seq,and protein analysis revealed that RAD23B upregulated Talin1 and Integrinsαv/β1,resulting in an activation of the PI3K/AKT signaling pathway.Moreover,RAD23B promoted MMP9 expression,contributing to enhanced invasive potential.Conclusion:RAD23B facilitates CRCmetastasis through activation of the Talin1/Integrinαv/β1/PI3K/AKT/MMP9 signaling axis.These results provide novel insights into the role of RAD23B in CRC progression and identify it as a potential therapeutic target.
基金supported by the National Natural Science Foundation of China(52473283,22193042,22125110,22435005,and U21A2069)the Natural Science Foundation of Fujian Province(2024J010037).
文摘Metal halide perovskites,particularly lead-based compounds,manifest great potential in X-ray detection due to their exceptional light absorption coefficients,superior carrier mobility and cost-effective preparation methods.Despite recent significant advancements,the limitations of lead toxicity hinder their further application and encourage the exploration of environmentally friendly alternatives.Herein,a novel lead-free two-dimensional(2D)copper-based halide perovskite(2FEA)_(2)CuCl_(4)(2FEA=2,2-difluoroethylamine)single crystal is developed,which exhibits a highly sensitive response to X-ray illumination.In particular,an inch-sized single crystal of(2FEA)_(2)CuCl_(4)with dimensions of up to 30×28×0.5 mm^(3)is grown using a low-cost cooling crystallization method.Direct X-ray detectors fabricated from these single crystals exhibited an exceptionally high sensitivity of 1106.44μC Gy_(air)^(−1)cm^(−2)at 10 V bias,approximately 43 times higher than that of the conventional radiation-sensitive semiconductor α-Se(~25μC Gy_(air)^(−1)cm^(−2)).Furthermore,(2FEA)2CuCl4 exhibits a stable baseline with a low dark current drift of 5.056×10^(−7)nA cm^(−1)s^(−1)V^(−1)and a low limit of detection of 130.1 nGy_(air)s^(−1)at 10 V bias.These findings manifest the great potential of 2D copper-based hybrid perovskites for next-generation highly sensitive X-ray detection,which sheds light on the rational crystal design of“green”radiation-sensitive hybrid perovskites.
基金supported by the National Nature Science Foundation of China(No.82203964)Science Talent Innovation Program of Shanghai Stomatological Hospital(No.SSH-2022-KJCX-B04),China.
文摘Objective:To investigate the contribution of YWHAZ gene on the radioresistance and metastasis ability of oral squamous cell carcinoma(OSCC)cells.Mathods:The relationship between the expression level of YWHAZ gene and the survival of head and neck squamous cell carcinoma(HNSC)patients was analyzed using Gene Expression Profiling Interactive Analysis(GEPIA)database.A radioresistance cell line(CAL-27R)was constructed by irradiating CAL-27 cells with frac-tional doses.Cell survival was measured by colony formation assay.Cell migration and invasion were detected by transwell assay.The formation of yH2AX foci was detected by immunofluorescence assay,The protein expressions were detected by Westemn blot assay.In some experiments,CAL-27R cells were effectively transferred with siRNA YWHAZ(siYWHAZ).Results:GEPIA database showed that the expression level of YWHAZ in HNSC tumors was higher than that in adjacent normal tissues,and the HNSC patients with higher level of YWHAZ had a shorter survival.In vitro experiments demonstrated that the expression of YWHAZ protein in CAL-27 cells was lower than HSC-3 cells(t=18.89,P<0.01)and radioresistant CAL-27R cells(t=25.70,P<0.01).Knockdown of YWHAZ gene significantly increased radiation-induced cell killing effect,apoptosis induction,and yH2AX foci formation inCAL-27R and HSC-3 cells.Moreover,siRNA YWHAZ transfection also reduced the invasion and migration abilities of the irradiated CAL 27R[t=21.09,P<0.01(migration);t=6.16,P<0.05(invasion)]and HSC-3 cells[t=34.53,P<0.001(migration);t=4.92,P<0.05(invasion)]and attenuated radiation-induced expressions of metastasis-related proteins,Conclusion:YWHAZ contributes to the radioresistance of oral squamous cells and thus it may applicable to be a potential target for OSOC radiotherapy.
