Objective: To investigate the effect of 5-aminolevulinic(ALA)-photodynamic therapy(PDT) on the expressions of MMP-9, MMP-13 and TIMP-1 of hypertrophic scar model in rabbit ears, and analyze the possible therapeutic me...Objective: To investigate the effect of 5-aminolevulinic(ALA)-photodynamic therapy(PDT) on the expressions of MMP-9, MMP-13 and TIMP-1 of hypertrophic scar model in rabbit ears, and analyze the possible therapeutic mechanisms of ALA-PDT treatment to hypertrophic scars of rabbit ears. Methods: The experimental animals were randomly divided into normal control, negative control, high concentration of ALA-PDT, low concentration of ALA-PDT and PDT groups. The latter three groups received ALA-PDT treatment or PDT treatment once a week for 3 weeks. The specimens of the rabbits were collected respectively 1, 2 and 3 months after treatment to be used for RT-PCR and Western-blot test. Results: 1, 2 and 3 months after PDT treatment, the expressions of MMP-9 and MMP-13(including mRNA and protein) in hypertrophic scar tissues of three treatment groups were significantly higher than those of the negative control group(P<0.01), and the expression of TIMP-1 mRNA and protein of three treatment groups were significantly lower than that of the negative control group(P<0.01). There were also significant differences between high-concentration ALA-PDT treatment group and the low one(P<0.05). Conclusion: ALA-PDT is effective in treating hypertrophic scars of rabbit ears, and its possible therapeutic mechanisms are that ALA-PDT treatment generates oxidation activation effect to activate the activity of MMPs and induces the photoaging of fibroblasts of hypertrophic scar tissues of rabbit ears to inhibit the activity of TIMPs, which causes the up-regulation of MMPs and the down-regulation of TIMPs. Because of this, the degradation of collagen and ECM is accelerated and the formation of scars is suppressed.展开更多
Objectives To investigatethe effects and involved mechanisms of scopolamine(Scop) on rabbit ear blood vessels. Methods Rabbitear blood vessels were desympathetic and desensoryinnervation with surgical operation. Diame...Objectives To investigatethe effects and involved mechanisms of scopolamine(Scop) on rabbit ear blood vessels. Methods Rabbitear blood vessels were desympathetic and desensoryinnervation with surgical operation. Diameters of dor-sal auricular arterial trunks in vivo were measuredwith a pair of compasses and the ruler in a dissectingmicroscope, and effluents from isolated ear underconstant perfusion pressure were recorded with a digi-tal drop-recorder. Results Intramuscular injectionof Scop 0.1 mg/kg made the diameter of denerveddorsal auricular arterial trunks, as well as that of in-nerved ones, significantly increased. Scop by itself,atthe maximal concentration (Cmax) of 3μM, 30μMand 300μM, did not alter the effluent flow from theisolated denervated rabbit ear, but chlorpromazine(CPZ), at Cmax of 1μM, acetylcholine (ACh), 0.25μM, all significantly increased the effluent flow, andnorepinephrine (NE), 0.1μM, significantly decreasedthe effluent. Scop, 3μM, did not affect ACh (0.25μM)-induced the increase of effluent flow, but Scop,30μM, alleviated the increase. Scop, 3μM, did notaffect NE (0.1μM)-induced the decrease of effluentflow, but Scop, 10, 30 and 100μM, significantly alle-viated the decrease. Conclusions The study sug-gests that Scop has no direct vasodilator effect. Thevasodilator effect of Scop is not due to the blockade ofmuscarinic receptor. However, Scop can dilate bloodvessels contracted by α_1-adrenoceptor activation.展开更多
In the thousand-year-old mural of Mogao Grottoes in Dunhuang,a special symbol tells the story of exchanges among civilisations.Three Rabbits Sharing Three Ears depicts three rabbits chasing each other,with each two sh...In the thousand-year-old mural of Mogao Grottoes in Dunhuang,a special symbol tells the story of exchanges among civilisations.Three Rabbits Sharing Three Ears depicts three rabbits chasing each other,with each two sharing one ear.It is said that its earliest version appeared in Dunhuang in the 6th century,and it had travelled across the desert along the ancient Silk Road,over mountains and rivers,leaping onto British ceramic tiles,integrating into Egyptian pottery and jumping cross the clock faces of German churches.展开更多
目的:他克莫司是临床广泛应用的一种药物,其对增生性瘢痕是否产生作用并无相关报道,为此提出并证实了他克莫司可以抑制兔耳瘢痕增生。方法:建立兔耳增生性瘢痕模型,选10只新西兰大耳白兔双耳腹侧用打孔器制作直径1cm圆形创面,伤后14天...目的:他克莫司是临床广泛应用的一种药物,其对增生性瘢痕是否产生作用并无相关报道,为此提出并证实了他克莫司可以抑制兔耳瘢痕增生。方法:建立兔耳增生性瘢痕模型,选10只新西兰大耳白兔双耳腹侧用打孔器制作直径1cm圆形创面,伤后14天创面上皮化后给药,每只兔左耳为空白对照组涂等剂量凡士林软膏,右耳为他克莫司治疗组。分别在伤后14天、21天2、8天3、5天和49天采集标本,行HE染色,观察形态学差异;Real-t i me PCR检测纤维化相关因子TGF-β1、TGF-β2、Col l agen-α1等的表达。结果:HE染色可见他克莫司组成纤维细胞数量及胶原纤维较对照组明显减少,PCR结果可见TGF-β1、TGF-β2及Col l agen-α1表达较对照组在各时间点均减少。结论:实验组较对照组瘢痕明显减轻,证明他克莫司显著抑制兔耳瘢痕增生,可作为临床上治疗及预防瘢痕增生的全新疗法。展开更多
基金Supported by the National Natural Science Foundation of China(30901298)
文摘Objective: To investigate the effect of 5-aminolevulinic(ALA)-photodynamic therapy(PDT) on the expressions of MMP-9, MMP-13 and TIMP-1 of hypertrophic scar model in rabbit ears, and analyze the possible therapeutic mechanisms of ALA-PDT treatment to hypertrophic scars of rabbit ears. Methods: The experimental animals were randomly divided into normal control, negative control, high concentration of ALA-PDT, low concentration of ALA-PDT and PDT groups. The latter three groups received ALA-PDT treatment or PDT treatment once a week for 3 weeks. The specimens of the rabbits were collected respectively 1, 2 and 3 months after treatment to be used for RT-PCR and Western-blot test. Results: 1, 2 and 3 months after PDT treatment, the expressions of MMP-9 and MMP-13(including mRNA and protein) in hypertrophic scar tissues of three treatment groups were significantly higher than those of the negative control group(P<0.01), and the expression of TIMP-1 mRNA and protein of three treatment groups were significantly lower than that of the negative control group(P<0.01). There were also significant differences between high-concentration ALA-PDT treatment group and the low one(P<0.05). Conclusion: ALA-PDT is effective in treating hypertrophic scars of rabbit ears, and its possible therapeutic mechanisms are that ALA-PDT treatment generates oxidation activation effect to activate the activity of MMPs and induces the photoaging of fibroblasts of hypertrophic scar tissues of rabbit ears to inhibit the activity of TIMPs, which causes the up-regulation of MMPs and the down-regulation of TIMPs. Because of this, the degradation of collagen and ECM is accelerated and the formation of scars is suppressed.
文摘Objectives To investigatethe effects and involved mechanisms of scopolamine(Scop) on rabbit ear blood vessels. Methods Rabbitear blood vessels were desympathetic and desensoryinnervation with surgical operation. Diameters of dor-sal auricular arterial trunks in vivo were measuredwith a pair of compasses and the ruler in a dissectingmicroscope, and effluents from isolated ear underconstant perfusion pressure were recorded with a digi-tal drop-recorder. Results Intramuscular injectionof Scop 0.1 mg/kg made the diameter of denerveddorsal auricular arterial trunks, as well as that of in-nerved ones, significantly increased. Scop by itself,atthe maximal concentration (Cmax) of 3μM, 30μMand 300μM, did not alter the effluent flow from theisolated denervated rabbit ear, but chlorpromazine(CPZ), at Cmax of 1μM, acetylcholine (ACh), 0.25μM, all significantly increased the effluent flow, andnorepinephrine (NE), 0.1μM, significantly decreasedthe effluent. Scop, 3μM, did not affect ACh (0.25μM)-induced the increase of effluent flow, but Scop,30μM, alleviated the increase. Scop, 3μM, did notaffect NE (0.1μM)-induced the decrease of effluentflow, but Scop, 10, 30 and 100μM, significantly alle-viated the decrease. Conclusions The study sug-gests that Scop has no direct vasodilator effect. Thevasodilator effect of Scop is not due to the blockade ofmuscarinic receptor. However, Scop can dilate bloodvessels contracted by α_1-adrenoceptor activation.
文摘In the thousand-year-old mural of Mogao Grottoes in Dunhuang,a special symbol tells the story of exchanges among civilisations.Three Rabbits Sharing Three Ears depicts three rabbits chasing each other,with each two sharing one ear.It is said that its earliest version appeared in Dunhuang in the 6th century,and it had travelled across the desert along the ancient Silk Road,over mountains and rivers,leaping onto British ceramic tiles,integrating into Egyptian pottery and jumping cross the clock faces of German churches.
文摘目的:他克莫司是临床广泛应用的一种药物,其对增生性瘢痕是否产生作用并无相关报道,为此提出并证实了他克莫司可以抑制兔耳瘢痕增生。方法:建立兔耳增生性瘢痕模型,选10只新西兰大耳白兔双耳腹侧用打孔器制作直径1cm圆形创面,伤后14天创面上皮化后给药,每只兔左耳为空白对照组涂等剂量凡士林软膏,右耳为他克莫司治疗组。分别在伤后14天、21天2、8天3、5天和49天采集标本,行HE染色,观察形态学差异;Real-t i me PCR检测纤维化相关因子TGF-β1、TGF-β2、Col l agen-α1等的表达。结果:HE染色可见他克莫司组成纤维细胞数量及胶原纤维较对照组明显减少,PCR结果可见TGF-β1、TGF-β2及Col l agen-α1表达较对照组在各时间点均减少。结论:实验组较对照组瘢痕明显减轻,证明他克莫司显著抑制兔耳瘢痕增生,可作为临床上治疗及预防瘢痕增生的全新疗法。
