Relaxin/insulin-like family peptide receptor 2 （RXFP2） is a robust candidate gene related to horn types in sheep. A series of independent genome-wide association studies have reported that RXFP2 underlies the existe...Relaxin/insulin-like family peptide receptor 2 （RXFP2） is a robust candidate gene related to horn types in sheep. A series of independent genome-wide association studies have reported that RXFP2 underlies the existence and lack of horns. In this study, High-Resolution Melting （HRM） analysis and DNA sequencing were employed to detect the polymorphism of RXFP2 gene in three sheep breeds from Qinghai-Tibet Plateau of China （ Tibetan sheep, Qinghai fine wool sheep and Alpine Merino sheep） and to determine the impacts of genotypes of RXFP2 on expression of horn phenotypes. The results showed that one single nucleotide pol- ymorphism （SNP） was identified as RXFP 2SNP c. 29389966 A 〉 G. The frequency of genotype AA in Alpine Merino ram （polled） was significantly higher than that in Tibetan ram （horned） and Qinghai fine wool ram （horned） Ix2（1, N= 421） = 72.25, P〈 0.001; xZ（1, N= 402） = 4.28, P〈 0.005）]; the fre- quency of genotype AA in Qinghai fine wool ewe （polled） was also much higher than that in Qinghai fine wool ewe （horned） and Tibetan ewe （horned） [x2（1, N = 196） = 42.04, P 〈 0. 001 ; x2 （ 1, N = 192） = 24. 69, P 〈 0. 005 ） ]. This mutation could potentially be exploited in marker-assisted selection （MAS） pro- grams within sheep industry to breed horned or polled animals.展开更多
Sheep(Ovis aries),among the first domesticated species,are now globally widespread and exhibit remarkable adaptability to diverse environments.In this study,we perform whole-genome sequencing of266 animals from 18 dis...Sheep(Ovis aries),among the first domesticated species,are now globally widespread and exhibit remarkable adaptability to diverse environments.In this study,we perform whole-genome sequencing of266 animals from 18 distinct Chinese sheep populations,each displaying unique phenotypes indicative of adaptation to varying environmental conditions.Integrating 131 environmental factors with single nucleotide polymorphism variations,we conduct a comprehensive genetic-environmental association analysis.This analysis identifies 35 key genes likely integral to the environmental adaptation of sheep.The functions of these genes include fat tail formation(HOXA10,HOXA11,JAZF1),wool characteristics(FER,FGF5,MITF,PDE4B),horn phenotypes(RXFP2),reproduction(HIBADH,TRIM71,C6H4orf22),and growth traits(ADGRL3,TRHDE).Notably,we observe a significant correlation between the frequency of missense mutations in the PAPSS2 and RXFP2 genes and variations in altitude.Our study reveals candidate genes for adaptive variation in sheep and demonstrates the diversity in how sheep adapt to their environment.展开更多
基金Supported by the Central Level Scientific Research Institutes for Basic R&D Special Fund Business(1610322015014)Earmarked Fund for Modern China Wool&Cashmere Technology Research System(nycytx-40-3)Engineering Research Center of Sheep and Goat Breeding of CAAS
文摘Relaxin/insulin-like family peptide receptor 2 （RXFP2） is a robust candidate gene related to horn types in sheep. A series of independent genome-wide association studies have reported that RXFP2 underlies the existence and lack of horns. In this study, High-Resolution Melting （HRM） analysis and DNA sequencing were employed to detect the polymorphism of RXFP2 gene in three sheep breeds from Qinghai-Tibet Plateau of China （ Tibetan sheep, Qinghai fine wool sheep and Alpine Merino sheep） and to determine the impacts of genotypes of RXFP2 on expression of horn phenotypes. The results showed that one single nucleotide pol- ymorphism （SNP） was identified as RXFP 2SNP c. 29389966 A 〉 G. The frequency of genotype AA in Alpine Merino ram （polled） was significantly higher than that in Tibetan ram （horned） and Qinghai fine wool ram （horned） Ix2（1, N= 421） = 72.25, P〈 0.001; xZ（1, N= 402） = 4.28, P〈 0.005）]; the fre- quency of genotype AA in Qinghai fine wool ewe （polled） was also much higher than that in Qinghai fine wool ewe （horned） and Tibetan ewe （horned） [x2（1, N = 196） = 42.04, P 〈 0. 001 ; x2 （ 1, N = 192） = 24. 69, P 〈 0. 005 ） ]. This mutation could potentially be exploited in marker-assisted selection （MAS） pro- grams within sheep industry to breed horned or polled animals.
基金supported by the National Natural Science Foundation of China(32222079,31961143021)the earmarked fund for the Modern Agro-industry Technology Research System(CARS-39-01)+1 种基金the Science and Technology Innovation Project of the Chinese Academy of Agricultural Sciences(ASTIP-IAS01)National Key Research and Development Program of China(2022YFF1000104-3)。
文摘Sheep(Ovis aries),among the first domesticated species,are now globally widespread and exhibit remarkable adaptability to diverse environments.In this study,we perform whole-genome sequencing of266 animals from 18 distinct Chinese sheep populations,each displaying unique phenotypes indicative of adaptation to varying environmental conditions.Integrating 131 environmental factors with single nucleotide polymorphism variations,we conduct a comprehensive genetic-environmental association analysis.This analysis identifies 35 key genes likely integral to the environmental adaptation of sheep.The functions of these genes include fat tail formation(HOXA10,HOXA11,JAZF1),wool characteristics(FER,FGF5,MITF,PDE4B),horn phenotypes(RXFP2),reproduction(HIBADH,TRIM71,C6H4orf22),and growth traits(ADGRL3,TRHDE).Notably,we observe a significant correlation between the frequency of missense mutations in the PAPSS2 and RXFP2 genes and variations in altitude.Our study reveals candidate genes for adaptive variation in sheep and demonstrates the diversity in how sheep adapt to their environment.
