Betalain,an economically valuable water-soluble natural plant pigment,is prized for its strong antioxidant activity,making it popular as a dietary supplement and a visual marker for plant transformation.However,market...Betalain,an economically valuable water-soluble natural plant pigment,is prized for its strong antioxidant activity,making it popular as a dietary supplement and a visual marker for plant transformation.However,market demand significantly outstrips current production capacity.This study reports the development of an efficient push-and-pull multigene strategy based on polycistronic expression and metabolic flux regulation to enhance betalain biosynthesis in transgenic maize(Zea mays L.)endosperm.We engineered a novel enhanced RUBY(eRUBY)system derived from the original polycistronic RUBY construct(CYP76AD1P2ADODA1P2ADOPA5GT unit,abbreviated CDG)by introducing arogenate dehydrogenase(ADHα)to increase the L-tyrosine substrate supply.All the genes were driven by the endosperm-specific promoter.Fusion of ADHαinto a single polycistronic eRUBY construct(CDGA)produced significantly higher betanin(6.88 mg g−1 dry weight)and isobetanin(1.81 mg g−1 dry weight)levels than in CDG+A,which stacked the ADHαcassette independently with CDG.The high betalain accumulation in CDGA lines(which also exhibited higher transgene copy number)resulted in a 2.85–7.58-fold improvement in endosperm antioxidant capacity compared to WT(versus 2.48–2.80-fold in CDG+A).Importantly,transgenic plants maintained a normal phenotype.Transcriptome and metabolome analyses further indicated that metabolism of phenylalanine,alanine,aspartate,and glutamate contributes to betalain production.Hybridization with sweet corn successfully created a high-sugar eRUBY maize variety.Collectively,these results demonstrate the successful development of a novel maize germplasm with significantly enhanced nutritional value through high betalain accumulation.展开更多
Ruby on Rails(简称ROR)是一个Web应用程序框架,以其能快速开发而备受敏捷开发实践者的推崇,它包含的重要概念有MVC模式、DSL、习惯约定优于配置、生成器、低重复等.以一个实际课题"中医药数据库共享平台"为例,研究如何应用RO...Ruby on Rails(简称ROR)是一个Web应用程序框架,以其能快速开发而备受敏捷开发实践者的推崇,它包含的重要概念有MVC模式、DSL、习惯约定优于配置、生成器、低重复等.以一个实际课题"中医药数据库共享平台"为例,研究如何应用ROR技术来满足一个实际需求的项目,涉及的技术有MVC模式、RESTful SOA、AJAX、Lighttpd&FCGI等.展开更多
基于Ruby语言构建的全新的Web开发框架Ruby on Rails,为Web应用程序开发提供了极其便捷的途径,近年来的迅速兴起成为了Web开发社区的焦点.采用支持敏捷开发的Ruby on Rails框架,研究并设计了一个新型体育门户网站,并重点介绍了网站各模...基于Ruby语言构建的全新的Web开发框架Ruby on Rails,为Web应用程序开发提供了极其便捷的途径,近年来的迅速兴起成为了Web开发社区的焦点.采用支持敏捷开发的Ruby on Rails框架,研究并设计了一个新型体育门户网站,并重点介绍了网站各模块的主要功能以及实现的关键技术.展开更多
Ruby on Rails是一个基于Ruby语言构建的全新Web应用程序架构,为Web应用程序开发提供了一种极其快捷的途径,正在和已经引起业界相当的兴趣.在给出了Ruby on Rails与轻量级J2EE架构Struts+Hibernate关键特性的基础上,从前端控制器、动作...Ruby on Rails是一个基于Ruby语言构建的全新Web应用程序架构,为Web应用程序开发提供了一种极其快捷的途径,正在和已经引起业界相当的兴趣.在给出了Ruby on Rails与轻量级J2EE架构Struts+Hibernate关键特性的基础上,从前端控制器、动作和模型,持久性框架三个方面对两种架构进行了比较.展开更多
以SNS、BLOG等为代表的web2.0应用中,经常需要上传图片,而且要对图片进行缩放处理,得到缩略图。使用ruby on rails开发框架,通过attachment_Fu插件可以方便地实现所有这些功能。这大大提高了web应用的开发效率,符合用户对web2.0应用持...以SNS、BLOG等为代表的web2.0应用中,经常需要上传图片,而且要对图片进行缩放处理,得到缩略图。使用ruby on rails开发框架,通过attachment_Fu插件可以方便地实现所有这些功能。这大大提高了web应用的开发效率,符合用户对web2.0应用持续开发、持续改进、持续创新的要求。展开更多
基金supported by grants from the Biological Breeding-National Science and Technology Major Project(2024ZD04077)the Invigorate the Seed Industry of Guangdong Province(2024-NPY-00-044)+3 种基金the National Natural Science Foundation of China(32272120)the Guangxi Science and Technology Major Project(GKAA24206023)the National Key Research and Development Program of China(2024YFF1000800)the Guangdong Basic Research Center of Excellence for Precise Breeding of Future Crops Major Project(FCBRCE-202502,FCBRCE-202504).
文摘Betalain,an economically valuable water-soluble natural plant pigment,is prized for its strong antioxidant activity,making it popular as a dietary supplement and a visual marker for plant transformation.However,market demand significantly outstrips current production capacity.This study reports the development of an efficient push-and-pull multigene strategy based on polycistronic expression and metabolic flux regulation to enhance betalain biosynthesis in transgenic maize(Zea mays L.)endosperm.We engineered a novel enhanced RUBY(eRUBY)system derived from the original polycistronic RUBY construct(CYP76AD1P2ADODA1P2ADOPA5GT unit,abbreviated CDG)by introducing arogenate dehydrogenase(ADHα)to increase the L-tyrosine substrate supply.All the genes were driven by the endosperm-specific promoter.Fusion of ADHαinto a single polycistronic eRUBY construct(CDGA)produced significantly higher betanin(6.88 mg g−1 dry weight)and isobetanin(1.81 mg g−1 dry weight)levels than in CDG+A,which stacked the ADHαcassette independently with CDG.The high betalain accumulation in CDGA lines(which also exhibited higher transgene copy number)resulted in a 2.85–7.58-fold improvement in endosperm antioxidant capacity compared to WT(versus 2.48–2.80-fold in CDG+A).Importantly,transgenic plants maintained a normal phenotype.Transcriptome and metabolome analyses further indicated that metabolism of phenylalanine,alanine,aspartate,and glutamate contributes to betalain production.Hybridization with sweet corn successfully created a high-sugar eRUBY maize variety.Collectively,these results demonstrate the successful development of a novel maize germplasm with significantly enhanced nutritional value through high betalain accumulation.
文摘Ruby on Rails(简称ROR)是一个Web应用程序框架,以其能快速开发而备受敏捷开发实践者的推崇,它包含的重要概念有MVC模式、DSL、习惯约定优于配置、生成器、低重复等.以一个实际课题"中医药数据库共享平台"为例,研究如何应用ROR技术来满足一个实际需求的项目,涉及的技术有MVC模式、RESTful SOA、AJAX、Lighttpd&FCGI等.
文摘Ruby on Rails是一个基于Ruby语言构建的全新Web应用程序架构,为Web应用程序开发提供了一种极其快捷的途径,正在和已经引起业界相当的兴趣.在给出了Ruby on Rails与轻量级J2EE架构Struts+Hibernate关键特性的基础上,从前端控制器、动作和模型,持久性框架三个方面对两种架构进行了比较.
文摘以SNS、BLOG等为代表的web2.0应用中,经常需要上传图片,而且要对图片进行缩放处理,得到缩略图。使用ruby on rails开发框架,通过attachment_Fu插件可以方便地实现所有这些功能。这大大提高了web应用的开发效率,符合用户对web2.0应用持续开发、持续改进、持续创新的要求。
