Malaria remains the most serious infectious disease and is one of the leading causes of death among children in malaria endemic areas.The development of malaria vaccines has been underway since 1960s.Significant progr...Malaria remains the most serious infectious disease and is one of the leading causes of death among children in malaria endemic areas.The development of malaria vaccines has been underway since 1960s.Significant progress in the development of vaccine has been made in the last decade.On 6 October 2021,World Health Organization recommended widespread use of the RTS,S/AS01 malaria vaccine.The level of acceptance of RTS,S/AS01 malaria vaccine is relatively low in middle-income countries.This might be because of lack of information regarding vaccine implementation in such countries.The proper and efficient execution of the malaria vaccination program necessitates careful consideration of each community's socio-cultural setting.The most prominent RTS,S/AS01 vaccine trial was conducted from 2009 to 2011 in which eleven sites in seven African countries participated.Results of the trial,published in 2015,provided a promising advance in the development of a malaria vaccine for African children.As of 2019,large-scale pilot studies of the vaccine have been conducted in Ghana,Kenya,and Malawi,involving several hundreds of thousands of infants.The RTS,S/AS01 vaccine shows modest efficacy against malaria and has a feasible mode of administration.Although there is increased risk of meningitis,cerebral malaria,pneumonia,anemia,febrile convulsions and gastroenteritis,the vaccine still has a feasible mode of administration and high cost effectiveness and can be easily implemented in resource-limited settings.展开更多
On December 9, 2014 the scientific research project ＂Developmentand commercial application of technology forultra-deep HDS of diesel （RTS）＂ jointly performed bythe SINOPEC Research Institute of Petroleum Processin...On December 9, 2014 the scientific research project ＂Developmentand commercial application of technology forultra-deep HDS of diesel （RTS）＂ jointly performed bythe SINOPEC Research Institute of Petroleum Processing（RIPP）, the Yanshan Petrochemical Branch Company（YPBC）, the Maoming Petrochemical Branch Companyand the Guangzhou Petrochemical Branch Company haspassed in Beijing the technical appraisal organized by theScience and Technology Division of the Sinopec Corp.展开更多
为建立猪传染性胃肠炎病毒(TGEV)SYBR Green Ⅰ荧光定量RT-PCR检测方法,根据GenBank收录的TGEV-Miller毒株的S基因序列,设计合成1对特异性引物,用RT-PCR方法从疫苗株中扩增TGEV S基因的部分保守片段,并克隆到pGEM-T Easy载体上,得到重...为建立猪传染性胃肠炎病毒(TGEV)SYBR Green Ⅰ荧光定量RT-PCR检测方法,根据GenBank收录的TGEV-Miller毒株的S基因序列,设计合成1对特异性引物,用RT-PCR方法从疫苗株中扩增TGEV S基因的部分保守片段,并克隆到pGEM-T Easy载体上,得到重组质粒作为荧光定量RT-PCR检测的标准模板,进行SYBR GreenⅠ荧光定量RT-PCR扩增,并制作标准曲线,建立TGEV的荧光定量RT-PCR检测方法。结果表明:该方法检测灵敏度可达30拷贝/μL,与猪圆环病毒、猪乙型脑炎病毒、猪伪狂犬病毒、猪瘟病毒和猪流感病毒和猪细小病毒不发生交叉反应,具有重复性好、特异性强、灵敏度高等优点,可用于临床TGEV感染的早期诊断以及分子流行病学调查。展开更多
文摘Malaria remains the most serious infectious disease and is one of the leading causes of death among children in malaria endemic areas.The development of malaria vaccines has been underway since 1960s.Significant progress in the development of vaccine has been made in the last decade.On 6 October 2021,World Health Organization recommended widespread use of the RTS,S/AS01 malaria vaccine.The level of acceptance of RTS,S/AS01 malaria vaccine is relatively low in middle-income countries.This might be because of lack of information regarding vaccine implementation in such countries.The proper and efficient execution of the malaria vaccination program necessitates careful consideration of each community's socio-cultural setting.The most prominent RTS,S/AS01 vaccine trial was conducted from 2009 to 2011 in which eleven sites in seven African countries participated.Results of the trial,published in 2015,provided a promising advance in the development of a malaria vaccine for African children.As of 2019,large-scale pilot studies of the vaccine have been conducted in Ghana,Kenya,and Malawi,involving several hundreds of thousands of infants.The RTS,S/AS01 vaccine shows modest efficacy against malaria and has a feasible mode of administration.Although there is increased risk of meningitis,cerebral malaria,pneumonia,anemia,febrile convulsions and gastroenteritis,the vaccine still has a feasible mode of administration and high cost effectiveness and can be easily implemented in resource-limited settings.
文摘On December 9, 2014 the scientific research project ＂Developmentand commercial application of technology forultra-deep HDS of diesel （RTS）＂ jointly performed bythe SINOPEC Research Institute of Petroleum Processing（RIPP）, the Yanshan Petrochemical Branch Company（YPBC）, the Maoming Petrochemical Branch Companyand the Guangzhou Petrochemical Branch Company haspassed in Beijing the technical appraisal organized by theScience and Technology Division of the Sinopec Corp.
