目的克隆小鼠IL-17(mIL-17)基因编码区并构建其真核载体。方法将10 ug LPS和等体积的完全福氏乳化后免疫C57BL/6小鼠,7 d后,取小鼠脾脏,提取脾细胞总RNA,通过RT-PCR扩增mIL-17基因全长编码区并将其克隆至pcDNA3.1载体。菌落PCR筛选阳性...目的克隆小鼠IL-17(mIL-17)基因编码区并构建其真核载体。方法将10 ug LPS和等体积的完全福氏乳化后免疫C57BL/6小鼠,7 d后,取小鼠脾脏,提取脾细胞总RNA,通过RT-PCR扩增mIL-17基因全长编码区并将其克隆至pcDNA3.1载体。菌落PCR筛选阳性克隆,限制性内切酶消化和序列分析进行鉴定。结果构建的重组载体中含有mIL-17基因编码区的全长序列,与NCBI公布的序列一致。结论获得mIL-17基因并构建了其真核表达载体,为研究IL-17在自身免疫性疾病中的作用奠定了基础。展开更多
This paper presents a comprehensive evaluation of system interactions in a battery electric vehicle caused by temperature sensitivity of permanent magnet synchronous machines(PMSM).An analytical model of a PMSM consid...This paper presents a comprehensive evaluation of system interactions in a battery electric vehicle caused by temperature sensitivity of permanent magnet synchronous machines(PMSM).An analytical model of a PMSM considering iron losses and thermal impact is implemented on a field programmable gate array suitable for hardware-in-the-loop testing.By the presented virtual prototyping approach,different machine characteristics defined by the design are used to parameterize the analytical model.The investigated temperature effect is understood as an interacting influence between machine character-istics and control,which are investigated in terms of torque generation,voltage utilization and efficiency under closed-loop condition in a vehicle environment.In particular,using a surface permanent magnet rotor and an interior permanent magnet rotor,the performance of both machine designs is analyzed by varying temperature-adjusted feedforward control strategies on the basis of a driving cycle from a racetrack.The comparison shows that the machine design with surface-mounted mag-nets is associated with higher temperature sensitivity.In this case,the temperature consideration in the feedforward control provides a14%loss reduction in closed-loop vehicle test operation.It can be summarized that the electromagnetic torque is less sensitive to a temperature variation with increasing reluctance.The presented development approach demonstrates the impact of interactions in electric powertrains without the need of real prototypes.展开更多
文摘目的克隆小鼠IL-17(mIL-17)基因编码区并构建其真核载体。方法将10 ug LPS和等体积的完全福氏乳化后免疫C57BL/6小鼠,7 d后,取小鼠脾脏,提取脾细胞总RNA,通过RT-PCR扩增mIL-17基因全长编码区并将其克隆至pcDNA3.1载体。菌落PCR筛选阳性克隆,限制性内切酶消化和序列分析进行鉴定。结果构建的重组载体中含有mIL-17基因编码区的全长序列,与NCBI公布的序列一致。结论获得mIL-17基因并构建了其真核表达载体,为研究IL-17在自身免疫性疾病中的作用奠定了基础。
文摘This paper presents a comprehensive evaluation of system interactions in a battery electric vehicle caused by temperature sensitivity of permanent magnet synchronous machines(PMSM).An analytical model of a PMSM considering iron losses and thermal impact is implemented on a field programmable gate array suitable for hardware-in-the-loop testing.By the presented virtual prototyping approach,different machine characteristics defined by the design are used to parameterize the analytical model.The investigated temperature effect is understood as an interacting influence between machine character-istics and control,which are investigated in terms of torque generation,voltage utilization and efficiency under closed-loop condition in a vehicle environment.In particular,using a surface permanent magnet rotor and an interior permanent magnet rotor,the performance of both machine designs is analyzed by varying temperature-adjusted feedforward control strategies on the basis of a driving cycle from a racetrack.The comparison shows that the machine design with surface-mounted mag-nets is associated with higher temperature sensitivity.In this case,the temperature consideration in the feedforward control provides a14%loss reduction in closed-loop vehicle test operation.It can be summarized that the electromagnetic torque is less sensitive to a temperature variation with increasing reluctance.The presented development approach demonstrates the impact of interactions in electric powertrains without the need of real prototypes.
