目的考察土鳖虫生物活性肽(active peptide of Eupolyphaga,APE)对高脂血症大鼠血脂和肠道菌群的调节情况。方法采用高脂饲料饲养SD大鼠诱导形成高脂血症大鼠模型,以辛伐他汀为阳性药,采用酶联免疫方法(ELISA)考察APE对高脂血症大鼠血...目的考察土鳖虫生物活性肽(active peptide of Eupolyphaga,APE)对高脂血症大鼠血脂和肠道菌群的调节情况。方法采用高脂饲料饲养SD大鼠诱导形成高脂血症大鼠模型,以辛伐他汀为阳性药,采用酶联免疫方法(ELISA)考察APE对高脂血症大鼠血脂的影响,同时运用16rS高通量测定技术(V4-V5)对APE干预模型大鼠肠道微生物菌群的结果进行测定。结果APE可显著降低模型大鼠血清中血脂指数,改善肝脏病理变化程度;同时肠道菌群结果显示,也可显著增加厚壁菌门中的柔菌属、乳酸杆菌属、双歧杆菌属的相对丰度,降低拟杆菌门、软壁菌门的瘤胃球菌属、普氏菌属、普雷沃菌属的相对丰度。结论APE具有降血脂的功效,同时也可修复高脂血症大鼠紊乱的肠道菌群谱,推测其作用机制为APE经肠道菌群干预脂质代谢途径发挥降血脂的功效。展开更多
Parkinson’s disease(PD)is a complex fatal chronic neurodegenerative disease most common in elderly people.The early genome-wide association studies(GWAS)found that the minor allele variant of PARK16 rs708730 polymorp...Parkinson’s disease(PD)is a complex fatal chronic neurodegenerative disease most common in elderly people.The early genome-wide association studies(GWAS)found that the minor allele variant of PARK16 rs708730 polymorphism is a significant protective factor for PD in Caucasian populations.However,these results cannot be repeated by the following studies in Caucasian populations and other populations.We considered that the inconsistency of the findings may be caused by the small-scale samples or the heterogeneity among different populations.Therefore,in this study,we synthesized the previous related GWAS studies through three authoritative sources,and used the large-scale samples(10,645 PD cases and 30,499 controls)to reevaluate the association between rs708730 polymorphism and PD.The results showed that there is no association between them in Asian ancestry population.While,in European ancestry population,we found that the minor allele variant(G)of rs708730 polymorphism is significantly associated with a decreased risk of PD.Collectively,our findings further verified the association of rs708730 with PD and show its genetic heterogeneity among different populations,which can help to develop a better understanding of the PD’s pathogenesis.展开更多
文摘目的考察土鳖虫生物活性肽(active peptide of Eupolyphaga,APE)对高脂血症大鼠血脂和肠道菌群的调节情况。方法采用高脂饲料饲养SD大鼠诱导形成高脂血症大鼠模型,以辛伐他汀为阳性药,采用酶联免疫方法(ELISA)考察APE对高脂血症大鼠血脂的影响,同时运用16rS高通量测定技术(V4-V5)对APE干预模型大鼠肠道微生物菌群的结果进行测定。结果APE可显著降低模型大鼠血清中血脂指数,改善肝脏病理变化程度;同时肠道菌群结果显示,也可显著增加厚壁菌门中的柔菌属、乳酸杆菌属、双歧杆菌属的相对丰度,降低拟杆菌门、软壁菌门的瘤胃球菌属、普氏菌属、普雷沃菌属的相对丰度。结论APE具有降血脂的功效,同时也可修复高脂血症大鼠紊乱的肠道菌群谱,推测其作用机制为APE经肠道菌群干预脂质代谢途径发挥降血脂的功效。
基金Lanzhou Science and Technology Bureau Program Funds(2017-RC-16,2018-1-110)Gansu Science and Technology Fund Grant(ID:17ZD2FA009)National Science and Technology Ministry(ID:2017ZX09101001).
文摘Parkinson’s disease(PD)is a complex fatal chronic neurodegenerative disease most common in elderly people.The early genome-wide association studies(GWAS)found that the minor allele variant of PARK16 rs708730 polymorphism is a significant protective factor for PD in Caucasian populations.However,these results cannot be repeated by the following studies in Caucasian populations and other populations.We considered that the inconsistency of the findings may be caused by the small-scale samples or the heterogeneity among different populations.Therefore,in this study,we synthesized the previous related GWAS studies through three authoritative sources,and used the large-scale samples(10,645 PD cases and 30,499 controls)to reevaluate the association between rs708730 polymorphism and PD.The results showed that there is no association between them in Asian ancestry population.While,in European ancestry population,we found that the minor allele variant(G)of rs708730 polymorphism is significantly associated with a decreased risk of PD.Collectively,our findings further verified the association of rs708730 with PD and show its genetic heterogeneity among different populations,which can help to develop a better understanding of the PD’s pathogenesis.
