To explore the structural characteristic of ribosomal protein S29 (rpS29) gene of Ailuropoda melanoleuca (giant panda), primers were designed based on the known nucleotide sequence of rpS29 genes to clone the cDNA and...To explore the structural characteristic of ribosomal protein S29 (rpS29) gene of Ailuropoda melanoleuca (giant panda), primers were designed based on the known nucleotide sequence of rpS29 genes to clone the cDNA and genomic sequences of this gene from giant panda by RT-PCR and PCR strategy respectively, and then the cloned cDNA and genomic sequences were sequenced and analyzed preliminarily. The results indicated that the cDNA fragment of the rpS29 from the giant panda is 205bp in size in length, containing an open reading frame (ORF) of 171bp, encoding 56 amino acids. The length of the genomic sequence is 1 598 bp, with three exons and two introns. The coding sequence shows a high degree of homology to those of Homo sapiens, Bos taurus, Rattus norvegicus and Mus musculus with 75%, 94.74%, 89.47% and 88.89% respectively, While all the homologies for amino acid sequences are high up to 100%. Primary structure analysis revealed that the molecular weight of the putative rpS29 protein is 6.68 KD, with a theoretical pI of 10.63. Based on topology prediction, there are three distinct types of functional sites in the rpS29 protein of giant panda.展开更多
基金financially supported by Application Foundation Project in Sichuan Province(2009JY0061)Youth Fund Project of Educational Committee of Sichuan province(09ZB088)
文摘To explore the structural characteristic of ribosomal protein S29 (rpS29) gene of Ailuropoda melanoleuca (giant panda), primers were designed based on the known nucleotide sequence of rpS29 genes to clone the cDNA and genomic sequences of this gene from giant panda by RT-PCR and PCR strategy respectively, and then the cloned cDNA and genomic sequences were sequenced and analyzed preliminarily. The results indicated that the cDNA fragment of the rpS29 from the giant panda is 205bp in size in length, containing an open reading frame (ORF) of 171bp, encoding 56 amino acids. The length of the genomic sequence is 1 598 bp, with three exons and two introns. The coding sequence shows a high degree of homology to those of Homo sapiens, Bos taurus, Rattus norvegicus and Mus musculus with 75%, 94.74%, 89.47% and 88.89% respectively, While all the homologies for amino acid sequences are high up to 100%. Primary structure analysis revealed that the molecular weight of the putative rpS29 protein is 6.68 KD, with a theoretical pI of 10.63. Based on topology prediction, there are three distinct types of functional sites in the rpS29 protein of giant panda.
