Fossil fuels are undoubtedly important, and drilling technology plays an important role in realizing fossil fuel exploration;therefore, the prediction and evaluation of drilling efficiency is a key research goal in th...Fossil fuels are undoubtedly important, and drilling technology plays an important role in realizing fossil fuel exploration;therefore, the prediction and evaluation of drilling efficiency is a key research goal in the industry. Limited by the unknown geological environment and complex operating procedures, the prediction and evaluation of drilling efficiency were very difficult before the introduction of machine learning algorithms. This review statistically analyses rate of penetration(ROP) prediction models established based on machine learning algorithms;establishes an overall framework including data collection, data preprocessing, model establishment, and accuracy evaluation;and compares the effectiveness of different algorithms in each link of the process. This review also compares the prediction accuracy of different machine learning models and traditional models commonly used in this field and demonstrates that machine learning models are the most effective technical means in current ROP prediction modeling.展开更多
细胞的极性生长可以作用于植物生长发育等一系列生物活动。其中花粉管的极性生长在被子植物有性生殖过程中发挥重要的作用。多种信号通路协同调控花粉管的极性生长,ROP (Rho-related GTPases of plants)所介导的信号途径是花粉管极性生...细胞的极性生长可以作用于植物生长发育等一系列生物活动。其中花粉管的极性生长在被子植物有性生殖过程中发挥重要的作用。多种信号通路协同调控花粉管的极性生长,ROP (Rho-related GTPases of plants)所介导的信号途径是花粉管极性生长调控网络中的主要参与者,也是植物其他细胞极性生长的关键调节因子。本研究将以拟南芥等植物的花粉管作为对象,对ROP信号途径进行简要叙述,旨在更全面地展现ROP在花粉管极性生长中的分子机制,为植物小G蛋白ROP的功能研究提供借鉴。展开更多
All of the Rho GTPase seems to form a special sub-family,because such a sub-family has so far only found in plants,and then named Rop GTPase,which directly involved in and regulated of muscle actin cytoskeletal reorga...All of the Rho GTPase seems to form a special sub-family,because such a sub-family has so far only found in plants,and then named Rop GTPase,which directly involved in and regulated of muscle actin cytoskeletal reorganization,such as a series of signal transductions.The efficient purification technology and the means of ROP GTPase in wheat are the key basis of the studies on its functions and prosperities.And it has a very important theoretical and practical significance in the signal transduction and F-act...展开更多
BACKGROUND Inflammatory bowel disease（IBD） is characterized by chronic and non-specific inflammation of the intestinal mucosa and mainly includes ulcerative colitis and Crohn’s disease.AIM To explore the beneficial...BACKGROUND Inflammatory bowel disease（IBD） is characterized by chronic and non-specific inflammation of the intestinal mucosa and mainly includes ulcerative colitis and Crohn’s disease.AIM To explore the beneficial effect of Toxo ROP16Ⅰ/Ⅲ-induced M2 phynotype macrophages in homeostasis of IBDs through downregulation of M1 inflammatory cells.METHODS RAW264.7 macrophages stimulated by lipopolysaccharide（LPS）（M1 cells） were co-cultured with Caco-2 cells as an inflammatory model of IBD in vitro.The expression of Toxo ROP16Ⅰ/Ⅲ was observed in RAW264.7 macrophages that were transfected with p EGFP-rop16Ⅰ/Ⅲ.The phenotypes of M2 and M1 macrophage cells were assessed by quantitative real-time reverse transcriptase polymerase chain reaction and the expression of tumor necrosis factor（TNF）-α,interleukin（IL）-1β,IL-6,transforming growth factor（TGF）-β1,IL-10,inducible nitric oxide synthase（i NOS）,and arginase-1（Arg-1） was detected.The expression of i NOS,Arg-1,signal transducer and activator of transcription 3（Stat3）,p-Stat3,Stat6,pStat6,programmed death ligand-2（PD-L2）,caspase-3,-8,and-9 was analyzed by Western blotting,and Griess assays were performed to detect nitric oxide（NO）.TNF-α,IL-1β,IL-6,TGF-β1,and IL-10 expression in the supernatants was detected by enzyme-linked immunosorbent assay,and Caco-2 cell apoptosis was determined by flow cytometry after mixing M1 cells with M2 cells in a Caco-2 cell co-culture system.RESULTS M1 cells exhibited significantly increased production of i NOS,NO,TNF-α,IL-1β,and IL-6,while Toxo ROP16Ⅰ/Ⅲ induced macrophage bias to M2 cells in vitro,showing increased expression of Arg-1,IL-10 and TGF-β1 and elevated production of p-Stat3 and p-Stat6.The mixed M1 and M2 cell culture induced by Toxo ROP16 Ⅰ/Ⅲ exhibited decreased production of NO and i NOS and upregulated expression of Arg-1 and PD-L2.Accordingly,Caco-2 cells became apoptotic,and apoptosis-associated proteins such as caspase-3,-8 and-9 were dampened during co-culture of M1 and M2 cells.Flow cytometry analysis showed that co-culture of M1 cells with Caco-2 cells facilitated the apoptosis of Caco-2 cells,but co-culture of M1 and M2 cells alleviated Caco-2 cell apoptosis.CONCLUSION Toxo ROP16 Ⅰ/Ⅲ-induced M2 macrophages inhibited apoptosis of Caco-2 cells caused by M1 macrophages.This finding may help gain a better understanding of the underlying mechanism and represent a promising therapeutic strategy for IBDs.展开更多
基金financially supported by CNOOC China Co., Ltd. Zhanjiang Branch (CNOOC-KJ135ZDXM3 8ZJ05ZJ)。
文摘Fossil fuels are undoubtedly important, and drilling technology plays an important role in realizing fossil fuel exploration;therefore, the prediction and evaluation of drilling efficiency is a key research goal in the industry. Limited by the unknown geological environment and complex operating procedures, the prediction and evaluation of drilling efficiency were very difficult before the introduction of machine learning algorithms. This review statistically analyses rate of penetration(ROP) prediction models established based on machine learning algorithms;establishes an overall framework including data collection, data preprocessing, model establishment, and accuracy evaluation;and compares the effectiveness of different algorithms in each link of the process. This review also compares the prediction accuracy of different machine learning models and traditional models commonly used in this field and demonstrates that machine learning models are the most effective technical means in current ROP prediction modeling.
文摘细胞的极性生长可以作用于植物生长发育等一系列生物活动。其中花粉管的极性生长在被子植物有性生殖过程中发挥重要的作用。多种信号通路协同调控花粉管的极性生长,ROP (Rho-related GTPases of plants)所介导的信号途径是花粉管极性生长调控网络中的主要参与者,也是植物其他细胞极性生长的关键调节因子。本研究将以拟南芥等植物的花粉管作为对象,对ROP信号途径进行简要叙述,旨在更全面地展现ROP在花粉管极性生长中的分子机制,为植物小G蛋白ROP的功能研究提供借鉴。
基金Supported by National Natural Science Foundation(30671061)Shanxi Province Natural Science Foundation(2008011059-1)~~
文摘All of the Rho GTPase seems to form a special sub-family,because such a sub-family has so far only found in plants,and then named Rop GTPase,which directly involved in and regulated of muscle actin cytoskeletal reorganization,such as a series of signal transductions.The efficient purification technology and the means of ROP GTPase in wheat are the key basis of the studies on its functions and prosperities.And it has a very important theoretical and practical significance in the signal transduction and F-act...
基金Supported by the National Natural Science Foundation of China,No.81471983the Key Research and Development Plan Project of Anhui Province,Department of Science and Technology 2019,No.201904a07020043+1 种基金the Key Project of Natural Science Research in the Universities of Anhui Provence,No.KJ2017A202the Research Fund Project of Anhui Institute of Transforming Medicine,No.2017zhyx04
文摘BACKGROUND Inflammatory bowel disease（IBD） is characterized by chronic and non-specific inflammation of the intestinal mucosa and mainly includes ulcerative colitis and Crohn’s disease.AIM To explore the beneficial effect of Toxo ROP16Ⅰ/Ⅲ-induced M2 phynotype macrophages in homeostasis of IBDs through downregulation of M1 inflammatory cells.METHODS RAW264.7 macrophages stimulated by lipopolysaccharide（LPS）（M1 cells） were co-cultured with Caco-2 cells as an inflammatory model of IBD in vitro.The expression of Toxo ROP16Ⅰ/Ⅲ was observed in RAW264.7 macrophages that were transfected with p EGFP-rop16Ⅰ/Ⅲ.The phenotypes of M2 and M1 macrophage cells were assessed by quantitative real-time reverse transcriptase polymerase chain reaction and the expression of tumor necrosis factor（TNF）-α,interleukin（IL）-1β,IL-6,transforming growth factor（TGF）-β1,IL-10,inducible nitric oxide synthase（i NOS）,and arginase-1（Arg-1） was detected.The expression of i NOS,Arg-1,signal transducer and activator of transcription 3（Stat3）,p-Stat3,Stat6,pStat6,programmed death ligand-2（PD-L2）,caspase-3,-8,and-9 was analyzed by Western blotting,and Griess assays were performed to detect nitric oxide（NO）.TNF-α,IL-1β,IL-6,TGF-β1,and IL-10 expression in the supernatants was detected by enzyme-linked immunosorbent assay,and Caco-2 cell apoptosis was determined by flow cytometry after mixing M1 cells with M2 cells in a Caco-2 cell co-culture system.RESULTS M1 cells exhibited significantly increased production of i NOS,NO,TNF-α,IL-1β,and IL-6,while Toxo ROP16Ⅰ/Ⅲ induced macrophage bias to M2 cells in vitro,showing increased expression of Arg-1,IL-10 and TGF-β1 and elevated production of p-Stat3 and p-Stat6.The mixed M1 and M2 cell culture induced by Toxo ROP16 Ⅰ/Ⅲ exhibited decreased production of NO and i NOS and upregulated expression of Arg-1 and PD-L2.Accordingly,Caco-2 cells became apoptotic,and apoptosis-associated proteins such as caspase-3,-8 and-9 were dampened during co-culture of M1 and M2 cells.Flow cytometry analysis showed that co-culture of M1 cells with Caco-2 cells facilitated the apoptosis of Caco-2 cells,but co-culture of M1 and M2 cells alleviated Caco-2 cell apoptosis.CONCLUSION Toxo ROP16 Ⅰ/Ⅲ-induced M2 macrophages inhibited apoptosis of Caco-2 cells caused by M1 macrophages.This finding may help gain a better understanding of the underlying mechanism and represent a promising therapeutic strategy for IBDs.
