Identification keys for terrestrial small mammals are often based on scientific details that require close examination of museum specimens.This paper builds on external morphological characteristics of the rodents rec...Identification keys for terrestrial small mammals are often based on scientific details that require close examination of museum specimens.This paper builds on external morphological characteristics of the rodents recorded through live trapping from 2011 to 2023 in Nyungwe National Park,a tropical rainforest in Rwanda,to formulate a taxonomic key suitable for both expert and non-expert researchers working in similar ecosystems across Africa.We reviewed the literature on taxonomic keys and field guide documents for small mammals to assess their practicality and identify gaps,with a special focus on their relevance to tropical regions and our study area.We then integrated our field records to harmonize this new development.We describe 23 rodent species,identified and confirmed using advanced taxonomic techniques,including DNA barcoding and voucher specimens.The study emphasizes that external features—particularly field photographs,body coloration(dorsal,ventral,and lateral views),and other distinctive anatomical traits—can serve as an effective field taxonomic key for rodents and other terrestrial small mammals,accessible to a broad scientific community.The paper also discusses the limitations of traditional dichotomous keys compared to short descriptions paired with photographic illustrations.The resulting key can be used as a template and is open to refinement as additional species are confirmed,re-assessed,or validated through advanced tools.Future studies may adapt this approach for other terrestrial small mammals and different locations across African tropical rainforests.展开更多
The microbiome of mammals has profound effects on host ftness,but the process,which drives the assembly and shift of mammalian microbiome remains poorly understood.To explore the patterns of small mammal microbial com...The microbiome of mammals has profound effects on host ftness,but the process,which drives the assembly and shift of mammalian microbiome remains poorly understood.To explore the patterns of small mammal microbial communities across host species and geographical sites and measure the relative contributions of different processes in driving assembly patterns,2 sympatric desert rodent species(Dipus sagitta and Meriones meridianus)were sampled from 2 geographically distant regions,which differed in the environment,followed by 16S rRNA gene sequencing.The microbiomes differed signifcantly between D.sagitta and M.meridianus,and linear mixed modeling(LMM)analysis revealed that microbial diversity was mostly affected by species rather than the environment.For each rodent species,the microbiome diversity and structure differed across geographical regions,with individuals from lower rainfall environments exhibiting greater diversity.The null modeling results suggested dispersal limitation and ecological drift rather than differential selective pressures acting on the microbiome.In addition,each group had a different core genus,suggesting that the taxonomic composition of the microbiome was shaped most strongly by stochastic processes.Our results suggest that variation in the microbiome between hosts,both within and among geographic rodent populations,is driven by bacterial dispersal and ecological drift rather than by differential selective pressures.These results elucidated the diversity patterns and assembly processes of bacterial microbiomes in small desert mammals.Deciphering the processes shaping the assembly of the microbial community is a premise for better understanding how the environment-host-microbe interactions of mammals are established and maintained,particularly in the context of increased environmental disturbances and global changes.展开更多
With the appearance of eutherian mammals,3 visual pigments were retained in their retina,mediating light sensitivity.Of them,rhodopsin is expressed in rod photoreceptor celis to conduct dim-light vision,while the othe...With the appearance of eutherian mammals,3 visual pigments were retained in their retina,mediating light sensitivity.Of them,rhodopsin is expressed in rod photoreceptor celis to conduct dim-light vision,while the other 2 visual pigments,middle/long wavelength-sensitive(M/LWS)and short wavelength-sensitive type 1(SWS1)visual pigments,expressed in cones are for color vision(Chi et al.2020).Murid rodents(order Rodentia:family Muridae),originated 17-22 million years ago(Mya)and represent more than 10%of living mammal species(Aghova et al.2018).Within these murid species,their SWS1 pigment retained the ancestral ultraviolet(UV)sensitivity,which is generally associated with a nocturnal lifestyle(Emerling et al.2015).In contrast,the spectral sensitivities of murid M/LWS are more diversifed,with wavelengths of maximum absorption(amax)ranging from 490 to 509 nm(Yokoyama et al.2008;Chi et al.2020).展开更多
In the Acknowledgements section of this article,the grant number"2020YFE0205900"relating to"National Key R&D Program of China"was missing,it has been included and the corrected Acknowledgements...In the Acknowledgements section of this article,the grant number"2020YFE0205900"relating to"National Key R&D Program of China"was missing,it has been included and the corrected Acknowledgements section is given below.展开更多
The development of rodent models that accurately reflect the pathogenesis of alcoholic liver disease(ALD)in humans is crucial for evaluating the nutritional intervention of food bioactive ingredients in ALD.Although v...The development of rodent models that accurately reflect the pathogenesis of alcoholic liver disease(ALD)in humans is crucial for evaluating the nutritional intervention of food bioactive ingredients in ALD.Although various models have been employed to establish ALD models over the past few decades,most successful cases are associated with high mortality rates,operational difficulties,and incompatibility formation mechanism compared to human ALD.However,the ALD models established by oral administration that simulate human drinking behavior often fail to induce significant liver damage.Therefore,it is imperative to explore simple and effective modes of oral administration for establishing ALD models consistent with the pathophysiological process of human ALD.Herein,we summarized the pathogenesis of ALD and discussed several issues related to construct ALD models with rodents(mainly mice and rats)by oral administration,including animal selection,animal feeding,alcohol intervention,and evaluation criteria.The purpose of this review is to provide a standardized and efficient formula for ALD modeling,so as to facilitate efficacy evaluation and mechanism analysis of food bioactive ingredients in ALD.展开更多
The cross-species infection of coronaviruses has resulted in several major epidemics since 2003.Porcine epidemic diarrhea virus(PEDV)is a devastating swine enteric coronavirus,which targets pigs as the only natural re...The cross-species infection of coronaviruses has resulted in several major epidemics since 2003.Porcine epidemic diarrhea virus(PEDV)is a devastating swine enteric coronavirus,which targets pigs as the only natural reservoir.In this study,the nucleic acid of PEDV was detected in rat fecal samples collected from pig farms.Further animal tests showed that PEDV can cause systemic infections in neonatal mice and rats via intracranial inoculation.The brain,lung,intestine and spleen were all targets for PEDV in rodents in contrast to the intestine being targeted in pigs.Morbidity and mortality vary via different infection routes.PEDV was also detectable in feces after infection,suggesting that the infected rodents were potential infectious sources.Moreover,the cerebral tropism of PEDV was verified in piglets via orally inoculation,which had not been identified before.In conclusion,our findings demonstrate that PEDV could cross the species barrier to infect mice and rats through different routes in experimental settings.Although it is highly devastating to piglets,PEDV changes the target organs and turns to be milder when meeting with new hosts.Based on these findings,more attention should be paid to the potential cross-species infection of PEDV.展开更多
The family Hepeviridae has seen an explosive expansion in its host range in recent years,yet the evolutionary trajectory of this zoonotic pathogen remains largely unknown.The emergence of rat hepatitis E virus(HEV)has...The family Hepeviridae has seen an explosive expansion in its host range in recent years,yet the evolutionary trajectory of this zoonotic pathogen remains largely unknown.The emergence of rat hepatitis E virus(HEV)has introduced a new public health threat due to its potential for zoonotic transmission.This study investigated2?464 wild small mammals spanning four animal orders,eight families,21 genera,and 37 species in Yunnan Province,China.Using broadly reactive reverse transcription-polymerase chain reaction(RT-PCR),we systematically screened the presence and prevalence of Orthohepevirus and identified 192 positive specimens from10 species,corresponding to an overall detection rate of7.79%.Next-generation sequencing enabled the recovery of 24 full-length genomic sequences from eight host species,including Bandicota bengalensis,Eothenomys eleusis,and Episoriculus caudatus,representing newly reported host species for Orthohepevirus strains.Phylogenetic and sequence analyses revealed extensive genetic diversity within orthohepeviruses infecting rodents and shrews.Notably,among the identified strains,20 were classified as Rocahepevirus ratti C1,two as C3,and one as Rocahepevirus eothenomi,while the remaining strain exhibited significant divergence,precluding classification.Evolutionary analyses highlighted close associations between orthohepeviruses and their respective host taxa,with distinct phylogenetic clustering patterns observed across different host orders.These findings emphasize the critical roles of co-speciation and cross-species transmission in shaping the evolutionary trajectories of the genera Paslahepevirus and Rocahepevirus.展开更多
Despite the well-established functions of neurotransmitters and their receptors in depression studies,the aetiology of depression remains unknown.Further research into the field of animal studies is required in order ...Despite the well-established functions of neurotransmitters and their receptors in depression studies,the aetiology of depression remains unknown.Further research into the field of animal studies is required in order to facilitate a more comprehensive understanding of the underlying mechanisms that contribute to the development of depression.While the potential of animal behaviour to elucidate the molecular underpinnings of depression remains to be elucidated,the establishment of animal models can facilitate the identification of analogous pathogenic pathways through the application of rigorous methodologies.Animal models that are suitable for simulating the illness state of human depression can be utilised to investigate the pathophysiology of depression and the development of novel antidepressant medications.Currently,there is an absence of an optimal animal model that can fully replicate the pathogenic pathways of human depression,which limits future research in this field.It is evident that stress constitutes the primary catalyst for the onset of depressive states,a phenomenon that has been observed in both human and animal subjects.From this standpoint,animal models of stress-induced depression should be better equipped to simulate the onset process of human depression.This study offers a comprehensive summary and analysis of the most frequently employed rodent models of depression,with a view to providing a more diverse range of models and resources for animal studies in the field of depression research.展开更多
The advances in the application of sterilizing techniques against pest rodents in China are introduced in the paper. The development of chemosterilants, improvements of botanic sterilants, production of new steroid ho...The advances in the application of sterilizing techniques against pest rodents in China are introduced in the paper. The development of chemosterilants, improvements of botanic sterilants, production of new steroid hormone sterilants, introduction of immunosterility and excellent properties of the sterilants are outlined. The 揝pace Occupation Theory?of sterile techniques is advanced after practice. The botanic sterilants with gossypol and trichosanthin as its main agents were screened and successfully applied in the large area control in the northern forest area of China. The safety of sterilants to non-target animals such as rats, rabbits, dogs, monkeys and chickens was summarized.展开更多
Objective To investigate the impact of impoundment and active public health interventions on rodent populations and rodent-borne diseases in the Three Gorges reservoir region from 1997 to 2012. Methods Surveillance da...Objective To investigate the impact of impoundment and active public health interventions on rodent populations and rodent-borne diseases in the Three Gorges reservoir region from 1997 to 2012. Methods Surveillance data from 1997 to 2012 were extracted from the Public Health Surveillance System of The Three Gorges established in 1997. Temporal changes in the incidences of hemorrhagic fever with renal syndrome （HFRS） and leptospirosis, rodent density, pathogen-carrying rates, and their correlations were analyzed. Results The average indoor and outdoor rodent densities decreased overall from 1997 to 2012. The average densities decreased by 47.72% （from 4.38% to 2.29%） and 39.68% （from 4.41% to 2.66%）, respectively, after impoundment （2003-2012） compared with before impoundment （1997-2002）. The average annual incidence rates of HFRS and leptospirosis were 0.29/100,000 and 0.52/100,000, respectively, and decreased by 85.74% （from 0.68/100,000 to 0.10/100,000） and 95.73% （from 1.47/100,000 to 0.06S/100,000）, respectively, after impoundment compared with before impoundment. Incidences of HFRS and leptospirosis appear to be positively correlated with rodent density in the reservoir area. Conclusion This study demonstrated that rodent density and incidences of rodent-borne diseases decreased and were maintained at low levels during construction of the Three Gorges dam. Measures that reduce rodent population densities could be effective in controlling rodent-borne diseases during large-scale hydraulic engineering construction.展开更多
We summarize theoretical approaches and practice of rodent pest control in Russia and former USSR during last 50 years. We review literature as well as original data to understand mechanisms of rodent populations reco...We summarize theoretical approaches and practice of rodent pest control in Russia and former USSR during last 50 years. We review literature as well as original data to understand mechanisms of rodent populations recovery after chemical control campaigns in urban areas, agricultural lands and natural foci of plague. Laboratory and field experiments indicate that inherent individual variation in behavioural, physiological and life-history traits provides survival of heterogeneous mix of individuals in residual population with increased resistance to poisonous baits and high reproductive potential that leads to fast recovery of a population. In a series of field experiments with various rodent and lagomorph species （Mus musculus, Rattus norvegicus, Meriones unguiculatus, M. meridianus, M. tamariscinus, Ochotona pallasii ） we have shown that patterns of recolonization of depopulated area and mechanisms of population recovery vary among species and depend on species-specific social organization. After control territorial and group-living species demonstrated an increase in mobility and affiliative and marking behaviour and a decrease in intraspecific aggression. The rate of recolonization of treated areas was high due to redistribution of survived individuals and immigration by neighbors. Population recovered to original level due to increased breeding performance and fecundity of both survived residents and immigrants. In contrast, socially-independent species exhibited minor changes in behaviour. Recolonization was mainly due to better survival and recruitment of youngs, so the rate of recolonization was low. Species-specificity of behavioural compensation mechanisms to control should be considered when developing ecologically based rodent management strategies展开更多
Bagged and dispersed Pyrinuron (a self-formulated rodenticide) were tested for control of Clethrionomus rutilus and Clethrionomus rufocanus in larch plantation, Pingshan area, Heilongjiang Province from Oct. 1998 to A...Bagged and dispersed Pyrinuron (a self-formulated rodenticide) were tested for control of Clethrionomus rutilus and Clethrionomus rufocanus in larch plantation, Pingshan area, Heilongjiang Province from Oct. 1998 to Apr. 1999. The results showed that the bagged Pyrinuron has good result for control of the two rodent pests. The density of rodents was reduced by 90.5% after application of bagged Pyrinuron. This application method has characteristics of long residual period, bait not going mould and saving labour force.展开更多
Since 2002,Hami City,Xinjiang Province,has implemented"construction project of rodent-free demonstration area".Artificial eagle frames are installed on the grassland to control rodent damage,receiving remark...Since 2002,Hami City,Xinjiang Province,has implemented"construction project of rodent-free demonstration area".Artificial eagle frames are installed on the grassland to control rodent damage,receiving remarkable control effect.The rodent density in the treatment area is below the threshold of hazard.Moreover,the implementation of the project increases the grassland biomass and improves the vegetation coverage,receiving good economic,ecological and social benefits.展开更多
China experiences serious agricultural damages from rodents, and, correspondingly, researchers in China are actively studying rodent biology and management. Following the popularization of ecologically-based rodent pe...China experiences serious agricultural damages from rodents, and, correspondingly, researchers in China are actively studying rodent biology and management. Following the popularization of ecologically-based rodent pest management and extension of the basic state policy of Ecological Civilization Construction in China, recent efforts in rodent control technologies are mainly focused on eliminating side effects of rodenticides and developing environmentally friendly techniques. Considering ecological functions of rodents to test their interaction with the environment is a promising design for understanding rodent populations in local ecosystems and for developing ecologically-based rodent management. Using ecological phenotypes and applying advanced techniques of molecular biology, genetics, and epigenetics might be useful for illustrating the deep regulatory mechanisms of fluctuations in rodent populations. However, facing the dual characteristics of rodents as pests and their roles as keystone species in many ecosystems, it is still a challenge to establish reasonable local thresholds to balance rodent management with the negative effects of rodent control on the environment in the future.展开更多
The road effect on small rodent population is investigated at 8 fragmented forest areas in the Baekdudaegan mountain range, South Korea in September 2001. We especially focused on the distribution and body condition o...The road effect on small rodent population is investigated at 8 fragmented forest areas in the Baekdudaegan mountain range, South Korea in September 2001. We especially focused on the distribution and body condition of small rodents near the roads. Korean field mouse (Apodemus peninsulae) seems to be more sensitive to the existence of a road than striped field mouse (Apodemus agrarius). Korean field mouse prefers interior forest area to around road. Striped field mouse is a habitat generalist and has wide distributional range around road, but Korean field mouse is forest-inhabiting species and their distribution is limited in forest area. These results suggest the effect of road is different on each small rodent species and their habitat preferences.展开更多
All extant species in the rodent family Spalacidae are subterranean and have evolved various traits for underground life.However,the phylogenomic relationships among its three subfamilies(Myospalacinae,Spalacinae,and ...All extant species in the rodent family Spalacidae are subterranean and have evolved various traits for underground life.However,the phylogenomic relationships among its three subfamilies(Myospalacinae,Spalacinae,and Rhizomyinae)and the molecular basis underlying their adaptations to underground life remain poorly understood.Here,we inferred the phylogenomic relationships among these subfamilies based on de novo sequencing the genome of the hoary bamboo rat(Rhizomys pruinosus).Analyses showed that 50%of the identified 11028 one-to-one orthologous protein-coding genes and the concatenated sequences of these orthologous genes strongly supported a sister relationship between Myospalacinae and Rhizomyinae.展开更多
Dear Editor,Cognitive impairment is a hallmark of neurodegenerative disorders such as Alzheimer’s disease（AD）and Parkinson’s disease.Growing evidence has demonstrated that cognitive impairment is closely associate...Dear Editor,Cognitive impairment is a hallmark of neurodegenerative disorders such as Alzheimer’s disease（AD）and Parkinson’s disease.Growing evidence has demonstrated that cognitive impairment is closely associated with insulin resistance.展开更多
Medication-related osteonecrosis of the jaw(MRONJ)is primarily associated with administering antiresorptive or antiangiogenic drugs.Despite significant research on MRONJ,its pathogenesis and effective treatments are s...Medication-related osteonecrosis of the jaw(MRONJ)is primarily associated with administering antiresorptive or antiangiogenic drugs.Despite significant research on MRONJ,its pathogenesis and effective treatments are still not fully understood.Animal models can be used to simulate the pathophysiological features of MRONJ,serving as standardized in vivo experimental platforms to explore the pathogenesis and therapies of MRONJ.Rodent models exhibit excellent effectiveness and high reproducibility in mimicking human MRONJ,but classical methods cannot achieve a complete replica of the pathogenesis of MRONJ.Modified rodent models have been reported with improvements for better mimicking of MRONJ onset in clinic.This review summarizes representative classical and modified rodent models of MRONJ created through various combinations of systemic drug induction and local stimulation and discusses their effectiveness and efficiency.Currently,there is a lack of a unified assessment system for MRONJ models,which hinders a standard definition of MRONJ-like lesions in rodents.Therefore,this review comprehensively summarizes assessment systems based on published peer-review articles,including new approaches in gross observation,histological assessments,radiographic assessments,and serological assessments.This review can serve as a reference for model establishment and evaluation in future preclinical studies on MRONJ.展开更多
Spinal cord injuries （SCI） in rodents have been created by laceration, contusion, compression, or intramedullary injection of toxic agents. The choice of an appropriate SCI model should be made for each study based ...Spinal cord injuries （SCI） in rodents have been created by laceration, contusion, compression, or intramedullary injection of toxic agents. The choice of an appropriate SCI model should be made for each study based on the experimental design, with care taken to avoid unintended complications such as hemorrhage. Technical comments will be made in this communication describing the 1） importance of vertebral stabi- lization, 2） injury preparation, and 3） landmarks to improve the preci- sion and reproducibility of the SCI.展开更多
<strong>Objectives:</strong> The digestive track of mice and humans has always been an integral part of the pathogenesis of the Trypanosomes but is constantly overlooked. This realization opens up complete...<strong>Objectives:</strong> The digestive track of mice and humans has always been an integral part of the pathogenesis of the Trypanosomes but is constantly overlooked. This realization opens up completely new strategies for the development of trypanosomes vaccines, allowing approaches that parenteral delivery forms would not permit. The target of the study was to compare the haematological changes and immunological responses of trypanosomiasis model systems (mice and rats) inoculated orally and intraperitoneally and to observe the afterward effect of a controlled drug [Isometamidium chloride (ISM)] in the restoration of these initial parameters. <strong>Methods:</strong> To achieve this, a total of 40 rodents (20 rats and 20 mice) were purchased, then grouped into two [sixteen younger (1 - 5 weeks) and older (7 - 15 weeks) groups each]. They were further sub-grouped into five each. Body weights, Parasitaemia and Packed Cell Volume (PCV) were taken before, after inoculation and after treatment with ISM at 4 mg/kg. <strong>Results:</strong> Based on presumptive clinical diagnosis, all rodents inoculated intraperitoneally showed clinical signs of fluctuations in weight, PCV and parasitaemia levels before, after inoculations and after treatment compared to those inoculated orally with a significant difference (P < 0.05) observed. Both young and older rodents also responded differently to the inoculants and to the different methods of inoculation. But more deaths were recorded among the mice when compared to the rats. <strong>Conclusion: </strong>Although these non-transgenic models would not have offered a completely new methods to vaccine development, their differences in response to various methods of inoculations is an indication of an exciting research processes and could offer desired results, particularly where transgenic rodents are employed.展开更多
文摘Identification keys for terrestrial small mammals are often based on scientific details that require close examination of museum specimens.This paper builds on external morphological characteristics of the rodents recorded through live trapping from 2011 to 2023 in Nyungwe National Park,a tropical rainforest in Rwanda,to formulate a taxonomic key suitable for both expert and non-expert researchers working in similar ecosystems across Africa.We reviewed the literature on taxonomic keys and field guide documents for small mammals to assess their practicality and identify gaps,with a special focus on their relevance to tropical regions and our study area.We then integrated our field records to harmonize this new development.We describe 23 rodent species,identified and confirmed using advanced taxonomic techniques,including DNA barcoding and voucher specimens.The study emphasizes that external features—particularly field photographs,body coloration(dorsal,ventral,and lateral views),and other distinctive anatomical traits—can serve as an effective field taxonomic key for rodents and other terrestrial small mammals,accessible to a broad scientific community.The paper also discusses the limitations of traditional dichotomous keys compared to short descriptions paired with photographic illustrations.The resulting key can be used as a template and is open to refinement as additional species are confirmed,re-assessed,or validated through advanced tools.Future studies may adapt this approach for other terrestrial small mammals and different locations across African tropical rainforests.
基金supported by grants from the Third Xinjiang Scientifc Expedition Program(Grant No.2022xjkk0205 to Lin Xia,Grant No.2021xjkk0604 to Jilong Cheng)the Joint Fund of National Natural Science Foundation of China(Grant No.U2003203 to Lin Xia)+1 种基金the Western Young Scholar Program-B of the Chinese Academy of Sciences(Grant No.2021-XBQNXZ-014 to Muyang Wang)the National Natural Science Foundation of China(Grant No.32170416 to Qisen Yang and Grant No.32370472 to Jilong Cheng).
文摘The microbiome of mammals has profound effects on host ftness,but the process,which drives the assembly and shift of mammalian microbiome remains poorly understood.To explore the patterns of small mammal microbial communities across host species and geographical sites and measure the relative contributions of different processes in driving assembly patterns,2 sympatric desert rodent species(Dipus sagitta and Meriones meridianus)were sampled from 2 geographically distant regions,which differed in the environment,followed by 16S rRNA gene sequencing.The microbiomes differed signifcantly between D.sagitta and M.meridianus,and linear mixed modeling(LMM)analysis revealed that microbial diversity was mostly affected by species rather than the environment.For each rodent species,the microbiome diversity and structure differed across geographical regions,with individuals from lower rainfall environments exhibiting greater diversity.The null modeling results suggested dispersal limitation and ecological drift rather than differential selective pressures acting on the microbiome.In addition,each group had a different core genus,suggesting that the taxonomic composition of the microbiome was shaped most strongly by stochastic processes.Our results suggest that variation in the microbiome between hosts,both within and among geographic rodent populations,is driven by bacterial dispersal and ecological drift rather than by differential selective pressures.These results elucidated the diversity patterns and assembly processes of bacterial microbiomes in small desert mammals.Deciphering the processes shaping the assembly of the microbial community is a premise for better understanding how the environment-host-microbe interactions of mammals are established and maintained,particularly in the context of increased environmental disturbances and global changes.
基金supported by the Natural Science Basic Research Program of Shaanxi(2022JQ-169)the China Postdoctoral Science Foundation(2021M702063)to S.S.,the National Natural Science Foundation of China(32270462)the Fundamental Research Funds for the Central Universities(GK202401001)to Y.L.
文摘With the appearance of eutherian mammals,3 visual pigments were retained in their retina,mediating light sensitivity.Of them,rhodopsin is expressed in rod photoreceptor celis to conduct dim-light vision,while the other 2 visual pigments,middle/long wavelength-sensitive(M/LWS)and short wavelength-sensitive type 1(SWS1)visual pigments,expressed in cones are for color vision(Chi et al.2020).Murid rodents(order Rodentia:family Muridae),originated 17-22 million years ago(Mya)and represent more than 10%of living mammal species(Aghova et al.2018).Within these murid species,their SWS1 pigment retained the ancestral ultraviolet(UV)sensitivity,which is generally associated with a nocturnal lifestyle(Emerling et al.2015).In contrast,the spectral sensitivities of murid M/LWS are more diversifed,with wavelengths of maximum absorption(amax)ranging from 490 to 509 nm(Yokoyama et al.2008;Chi et al.2020).
文摘In the Acknowledgements section of this article,the grant number"2020YFE0205900"relating to"National Key R&D Program of China"was missing,it has been included and the corrected Acknowledgements section is given below.
基金supported by the National Natural Science Foundation of China(32430083).
文摘The development of rodent models that accurately reflect the pathogenesis of alcoholic liver disease(ALD)in humans is crucial for evaluating the nutritional intervention of food bioactive ingredients in ALD.Although various models have been employed to establish ALD models over the past few decades,most successful cases are associated with high mortality rates,operational difficulties,and incompatibility formation mechanism compared to human ALD.However,the ALD models established by oral administration that simulate human drinking behavior often fail to induce significant liver damage.Therefore,it is imperative to explore simple and effective modes of oral administration for establishing ALD models consistent with the pathophysiological process of human ALD.Herein,we summarized the pathogenesis of ALD and discussed several issues related to construct ALD models with rodents(mainly mice and rats)by oral administration,including animal selection,animal feeding,alcohol intervention,and evaluation criteria.The purpose of this review is to provide a standardized and efficient formula for ALD modeling,so as to facilitate efficacy evaluation and mechanism analysis of food bioactive ingredients in ALD.
基金supported by the Basic Research Innovation Group Project of Gansu Province(No.25JRRA434)the National Natural Science Foundation of China(U22A20522).
文摘The cross-species infection of coronaviruses has resulted in several major epidemics since 2003.Porcine epidemic diarrhea virus(PEDV)is a devastating swine enteric coronavirus,which targets pigs as the only natural reservoir.In this study,the nucleic acid of PEDV was detected in rat fecal samples collected from pig farms.Further animal tests showed that PEDV can cause systemic infections in neonatal mice and rats via intracranial inoculation.The brain,lung,intestine and spleen were all targets for PEDV in rodents in contrast to the intestine being targeted in pigs.Morbidity and mortality vary via different infection routes.PEDV was also detectable in feces after infection,suggesting that the infected rodents were potential infectious sources.Moreover,the cerebral tropism of PEDV was verified in piglets via orally inoculation,which had not been identified before.In conclusion,our findings demonstrate that PEDV could cross the species barrier to infect mice and rats through different routes in experimental settings.Although it is highly devastating to piglets,PEDV changes the target organs and turns to be milder when meeting with new hosts.Based on these findings,more attention should be paid to the potential cross-species infection of PEDV.
基金supported by the National Natural Science Foundation of China (U2002218,81874274)Yunnan Health Training Project of High Level Talents (L-2017027)+3 种基金Project of Cross-border Control and Quarantine Innovation Group of Zoonosis of Dali University (ZKPY2019302)to Y.Z.ZOpen Project of Yunnan Key Laboratory of Biodiversity Information (BIKF22-02)Youth Innovation Promotion Association of the Chinese Academy of SciencesYunnan Revitalization Talent Support Program Young Talent Project to X.L.Y。
文摘The family Hepeviridae has seen an explosive expansion in its host range in recent years,yet the evolutionary trajectory of this zoonotic pathogen remains largely unknown.The emergence of rat hepatitis E virus(HEV)has introduced a new public health threat due to its potential for zoonotic transmission.This study investigated2?464 wild small mammals spanning four animal orders,eight families,21 genera,and 37 species in Yunnan Province,China.Using broadly reactive reverse transcription-polymerase chain reaction(RT-PCR),we systematically screened the presence and prevalence of Orthohepevirus and identified 192 positive specimens from10 species,corresponding to an overall detection rate of7.79%.Next-generation sequencing enabled the recovery of 24 full-length genomic sequences from eight host species,including Bandicota bengalensis,Eothenomys eleusis,and Episoriculus caudatus,representing newly reported host species for Orthohepevirus strains.Phylogenetic and sequence analyses revealed extensive genetic diversity within orthohepeviruses infecting rodents and shrews.Notably,among the identified strains,20 were classified as Rocahepevirus ratti C1,two as C3,and one as Rocahepevirus eothenomi,while the remaining strain exhibited significant divergence,precluding classification.Evolutionary analyses highlighted close associations between orthohepeviruses and their respective host taxa,with distinct phylogenetic clustering patterns observed across different host orders.These findings emphasize the critical roles of co-speciation and cross-species transmission in shaping the evolutionary trajectories of the genera Paslahepevirus and Rocahepevirus.
文摘Despite the well-established functions of neurotransmitters and their receptors in depression studies,the aetiology of depression remains unknown.Further research into the field of animal studies is required in order to facilitate a more comprehensive understanding of the underlying mechanisms that contribute to the development of depression.While the potential of animal behaviour to elucidate the molecular underpinnings of depression remains to be elucidated,the establishment of animal models can facilitate the identification of analogous pathogenic pathways through the application of rigorous methodologies.Animal models that are suitable for simulating the illness state of human depression can be utilised to investigate the pathophysiology of depression and the development of novel antidepressant medications.Currently,there is an absence of an optimal animal model that can fully replicate the pathogenic pathways of human depression,which limits future research in this field.It is evident that stress constitutes the primary catalyst for the onset of depressive states,a phenomenon that has been observed in both human and animal subjects.From this standpoint,animal models of stress-induced depression should be better equipped to simulate the onset process of human depression.This study offers a comprehensive summary and analysis of the most frequently employed rodent models of depression,with a view to providing a more diverse range of models and resources for animal studies in the field of depression research.
文摘The advances in the application of sterilizing techniques against pest rodents in China are introduced in the paper. The development of chemosterilants, improvements of botanic sterilants, production of new steroid hormone sterilants, introduction of immunosterility and excellent properties of the sterilants are outlined. The 揝pace Occupation Theory?of sterile techniques is advanced after practice. The botanic sterilants with gossypol and trichosanthin as its main agents were screened and successfully applied in the large area control in the northern forest area of China. The safety of sterilants to non-target animals such as rats, rabbits, dogs, monkeys and chickens was summarized.
基金supported by grants from the State Council Three Gorges Project Construction Committee Executive Office(No.SX[98]-05KHB/JSSX[2004]-018+3 种基金SX[2006]-003SX[2007]-002SX[2009]-020)JJ[2011]-030)
文摘Objective To investigate the impact of impoundment and active public health interventions on rodent populations and rodent-borne diseases in the Three Gorges reservoir region from 1997 to 2012. Methods Surveillance data from 1997 to 2012 were extracted from the Public Health Surveillance System of The Three Gorges established in 1997. Temporal changes in the incidences of hemorrhagic fever with renal syndrome （HFRS） and leptospirosis, rodent density, pathogen-carrying rates, and their correlations were analyzed. Results The average indoor and outdoor rodent densities decreased overall from 1997 to 2012. The average densities decreased by 47.72% （from 4.38% to 2.29%） and 39.68% （from 4.41% to 2.66%）, respectively, after impoundment （2003-2012） compared with before impoundment （1997-2002）. The average annual incidence rates of HFRS and leptospirosis were 0.29/100,000 and 0.52/100,000, respectively, and decreased by 85.74% （from 0.68/100,000 to 0.10/100,000） and 95.73% （from 1.47/100,000 to 0.06S/100,000）, respectively, after impoundment compared with before impoundment. Incidences of HFRS and leptospirosis appear to be positively correlated with rodent density in the reservoir area. Conclusion This study demonstrated that rodent density and incidences of rodent-borne diseases decreased and were maintained at low levels during construction of the Three Gorges dam. Measures that reduce rodent population densities could be effective in controlling rodent-borne diseases during large-scale hydraulic engineering construction.
文摘We summarize theoretical approaches and practice of rodent pest control in Russia and former USSR during last 50 years. We review literature as well as original data to understand mechanisms of rodent populations recovery after chemical control campaigns in urban areas, agricultural lands and natural foci of plague. Laboratory and field experiments indicate that inherent individual variation in behavioural, physiological and life-history traits provides survival of heterogeneous mix of individuals in residual population with increased resistance to poisonous baits and high reproductive potential that leads to fast recovery of a population. In a series of field experiments with various rodent and lagomorph species （Mus musculus, Rattus norvegicus, Meriones unguiculatus, M. meridianus, M. tamariscinus, Ochotona pallasii ） we have shown that patterns of recolonization of depopulated area and mechanisms of population recovery vary among species and depend on species-specific social organization. After control territorial and group-living species demonstrated an increase in mobility and affiliative and marking behaviour and a decrease in intraspecific aggression. The rate of recolonization of treated areas was high due to redistribution of survived individuals and immigration by neighbors. Population recovered to original level due to increased breeding performance and fecundity of both survived residents and immigrants. In contrast, socially-independent species exhibited minor changes in behaviour. Recolonization was mainly due to better survival and recruitment of youngs, so the rate of recolonization was low. Species-specificity of behavioural compensation mechanisms to control should be considered when developing ecologically based rodent management strategies
文摘Bagged and dispersed Pyrinuron (a self-formulated rodenticide) were tested for control of Clethrionomus rutilus and Clethrionomus rufocanus in larch plantation, Pingshan area, Heilongjiang Province from Oct. 1998 to Apr. 1999. The results showed that the bagged Pyrinuron has good result for control of the two rodent pests. The density of rodents was reduced by 90.5% after application of bagged Pyrinuron. This application method has characteristics of long residual period, bait not going mould and saving labour force.
文摘Since 2002,Hami City,Xinjiang Province,has implemented"construction project of rodent-free demonstration area".Artificial eagle frames are installed on the grassland to control rodent damage,receiving remarkable control effect.The rodent density in the treatment area is below the threshold of hazard.Moreover,the implementation of the project increases the grassland biomass and improves the vegetation coverage,receiving good economic,ecological and social benefits.
基金supported by the National Natural Science Foundation of China(31401761)the Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences
文摘China experiences serious agricultural damages from rodents, and, correspondingly, researchers in China are actively studying rodent biology and management. Following the popularization of ecologically-based rodent pest management and extension of the basic state policy of Ecological Civilization Construction in China, recent efforts in rodent control technologies are mainly focused on eliminating side effects of rodenticides and developing environmentally friendly techniques. Considering ecological functions of rodents to test their interaction with the environment is a promising design for understanding rodent populations in local ecosystems and for developing ecologically-based rodent management. Using ecological phenotypes and applying advanced techniques of molecular biology, genetics, and epigenetics might be useful for illustrating the deep regulatory mechanisms of fluctuations in rodent populations. However, facing the dual characteristics of rodents as pests and their roles as keystone species in many ecosystems, it is still a challenge to establish reasonable local thresholds to balance rodent management with the negative effects of rodent control on the environment in the future.
文摘The road effect on small rodent population is investigated at 8 fragmented forest areas in the Baekdudaegan mountain range, South Korea in September 2001. We especially focused on the distribution and body condition of small rodents near the roads. Korean field mouse (Apodemus peninsulae) seems to be more sensitive to the existence of a road than striped field mouse (Apodemus agrarius). Korean field mouse prefers interior forest area to around road. Striped field mouse is a habitat generalist and has wide distributional range around road, but Korean field mouse is forest-inhabiting species and their distribution is limited in forest area. These results suggest the effect of road is different on each small rodent species and their habitat preferences.
基金supported by the National Natural Science Foundation of China(31922010,31871270,31260087,31460561)China National Center for Biotechnology Development(2020YFC0847000)+3 种基金Key Research Program of the Chinese Academy of Sciences(KJZD-SW-L11)Yunnan Provincial Science and Technology Department(2019FI008)Yunnan Applied Basic Research Projects(2018FB050)Training Funds of Ten-thousand Talents Program of Yunnan Province(to L.Z.T.)。
文摘All extant species in the rodent family Spalacidae are subterranean and have evolved various traits for underground life.However,the phylogenomic relationships among its three subfamilies(Myospalacinae,Spalacinae,and Rhizomyinae)and the molecular basis underlying their adaptations to underground life remain poorly understood.Here,we inferred the phylogenomic relationships among these subfamilies based on de novo sequencing the genome of the hoary bamboo rat(Rhizomys pruinosus).Analyses showed that 50%of the identified 11028 one-to-one orthologous protein-coding genes and the concatenated sequences of these orthologous genes strongly supported a sister relationship between Myospalacinae and Rhizomyinae.
基金supported by the National Natural Science Foundation of China (81471037 and 81770841)the "Six Kinds of Talents Summit" of Jiangsu Province, China (SWYY-051)+1 种基金Basic Research Program of Education Department of Jiangsu Province, China (14KJA180006)the Program for New Technology of Clinical Diagnosis and Treatment at Nantong (MS22016024)
文摘Dear Editor,Cognitive impairment is a hallmark of neurodegenerative disorders such as Alzheimer’s disease（AD）and Parkinson’s disease.Growing evidence has demonstrated that cognitive impairment is closely associated with insulin resistance.
基金supported by the National Natural Science Foundation of China(No.81921002,No.81900970)Young Physician Innovation Team Project(No.QC202003)from Ninth People’s Hospital,Shanghai Jiao Tong University School of MedicineShanghai Sailing Program(19YF1426000)jointly。
文摘Medication-related osteonecrosis of the jaw(MRONJ)is primarily associated with administering antiresorptive or antiangiogenic drugs.Despite significant research on MRONJ,its pathogenesis and effective treatments are still not fully understood.Animal models can be used to simulate the pathophysiological features of MRONJ,serving as standardized in vivo experimental platforms to explore the pathogenesis and therapies of MRONJ.Rodent models exhibit excellent effectiveness and high reproducibility in mimicking human MRONJ,but classical methods cannot achieve a complete replica of the pathogenesis of MRONJ.Modified rodent models have been reported with improvements for better mimicking of MRONJ onset in clinic.This review summarizes representative classical and modified rodent models of MRONJ created through various combinations of systemic drug induction and local stimulation and discusses their effectiveness and efficiency.Currently,there is a lack of a unified assessment system for MRONJ models,which hinders a standard definition of MRONJ-like lesions in rodents.Therefore,this review comprehensively summarizes assessment systems based on published peer-review articles,including new approaches in gross observation,histological assessments,radiographic assessments,and serological assessments.This review can serve as a reference for model establishment and evaluation in future preclinical studies on MRONJ.
文摘Spinal cord injuries （SCI） in rodents have been created by laceration, contusion, compression, or intramedullary injection of toxic agents. The choice of an appropriate SCI model should be made for each study based on the experimental design, with care taken to avoid unintended complications such as hemorrhage. Technical comments will be made in this communication describing the 1） importance of vertebral stabi- lization, 2） injury preparation, and 3） landmarks to improve the preci- sion and reproducibility of the SCI.
文摘<strong>Objectives:</strong> The digestive track of mice and humans has always been an integral part of the pathogenesis of the Trypanosomes but is constantly overlooked. This realization opens up completely new strategies for the development of trypanosomes vaccines, allowing approaches that parenteral delivery forms would not permit. The target of the study was to compare the haematological changes and immunological responses of trypanosomiasis model systems (mice and rats) inoculated orally and intraperitoneally and to observe the afterward effect of a controlled drug [Isometamidium chloride (ISM)] in the restoration of these initial parameters. <strong>Methods:</strong> To achieve this, a total of 40 rodents (20 rats and 20 mice) were purchased, then grouped into two [sixteen younger (1 - 5 weeks) and older (7 - 15 weeks) groups each]. They were further sub-grouped into five each. Body weights, Parasitaemia and Packed Cell Volume (PCV) were taken before, after inoculation and after treatment with ISM at 4 mg/kg. <strong>Results:</strong> Based on presumptive clinical diagnosis, all rodents inoculated intraperitoneally showed clinical signs of fluctuations in weight, PCV and parasitaemia levels before, after inoculations and after treatment compared to those inoculated orally with a significant difference (P < 0.05) observed. Both young and older rodents also responded differently to the inoculants and to the different methods of inoculation. But more deaths were recorded among the mice when compared to the rats. <strong>Conclusion: </strong>Although these non-transgenic models would not have offered a completely new methods to vaccine development, their differences in response to various methods of inoculations is an indication of an exciting research processes and could offer desired results, particularly where transgenic rodents are employed.
