Objective To investigate the distribution of mitochondria-associated molecule mRNA in cerebellar Purkinje cells.Methods Transgenic technology was used to prepare Pcp2-tdTomato mouse labeled Purkinje cells.The mRNAs of...Objective To investigate the distribution of mitochondria-associated molecule mRNA in cerebellar Purkinje cells.Methods Transgenic technology was used to prepare Pcp2-tdTomato mouse labeled Purkinje cells.The mRNAs of mitochondria-associated molecules Mfn2,Mfn1,Ucp4,Drp1,Ucp2,Mcu and Nclx were detected in situ by RNAscope technique.Results The results of this study showed that Mfn2,Mfn1,Ucp4 and Drp1 were highly expressed in Purkinje cell body(Bin 3>10%).However,Ucp2,Mcu and Nclx were less expressed.In addition,Mfn2,Mfn1,Ucp4,Drp1,Ucp2,Mcu and Nclx were less expressed in dendrites.Conclusion Current research focuses on developing new and more specific molecules to regulate the activity of Purkinje cells and opening therapeutic windows for Purkinje cells related diseases.The molecule identification of potential drug targets,mechanisms of action,and structural basis of their activity will crucially promote preclinical development.展开更多
The oxytocin receptor(OXTR)has garnered increasing attention for its role in regulating both mature behaviors and brain development.It has been established that OXTR mediates a range of effects that are regionspecifc ...The oxytocin receptor(OXTR)has garnered increasing attention for its role in regulating both mature behaviors and brain development.It has been established that OXTR mediates a range of effects that are regionspecifc or period-specifc.However,the current studies of OXTR expression patterns in mice only provide limited help due to limitations in resolution.Therefore,our objective was to generate a comprehensive,high-resolution spatiotemporal expression map of Oxtr mRNA across the entire developing mouse brain.We applied RNAscope in situ hybridization to investigate the spatiotemporal expression pattern of Oxtr in the brains of male mice at six distinct postnatal developmental stages(P7,P14,P21,P28,P42,P56).We provide detailed descriptions of Oxtr expression patterns in key brain regions,including the cortex,basal forebrain,hippocampus,and amygdaloid complex,with a focus on the precise localization of Oxtr+cells and the variance of expression between diferent neurons.Furthermore,we identifed some neuronal populations with high Oxtr expression levels that have been little studied,including glutamatergic neurons in the ventral dentate gyrus,Vgat+Oxtr+cells in the basal forebrain,and GABAergic neurons in layers 4/5 of the cortex.Our study provides a novel perspective for understanding the distribution of Oxtr and encourages further investigations into its functions.展开更多
基金Military Medical Advancement Program for Air Force Medical University(2020SWAQ04)Shaanxi Innovation Ability Support Plan(2023-CX-PT-33)+2 种基金Shaanxi Province Natural Science Basic Research Program(2024JC-ZDXM-60,2022JQ-820)New Clinical Technology of Xijing Hospital(2023XJSY27)National Natural Science Foundation of China(82201627)。
文摘Objective To investigate the distribution of mitochondria-associated molecule mRNA in cerebellar Purkinje cells.Methods Transgenic technology was used to prepare Pcp2-tdTomato mouse labeled Purkinje cells.The mRNAs of mitochondria-associated molecules Mfn2,Mfn1,Ucp4,Drp1,Ucp2,Mcu and Nclx were detected in situ by RNAscope technique.Results The results of this study showed that Mfn2,Mfn1,Ucp4 and Drp1 were highly expressed in Purkinje cell body(Bin 3>10%).However,Ucp2,Mcu and Nclx were less expressed.In addition,Mfn2,Mfn1,Ucp4,Drp1,Ucp2,Mcu and Nclx were less expressed in dendrites.Conclusion Current research focuses on developing new and more specific molecules to regulate the activity of Purkinje cells and opening therapeutic windows for Purkinje cells related diseases.The molecule identification of potential drug targets,mechanisms of action,and structural basis of their activity will crucially promote preclinical development.
基金supported by the National Science Fund for Distinguished Young Scholars(32325025)the STI2030-Major Projects(2021ZD0202500)+2 种基金the National Key Research and Development Program of China(2021YFA1101801)the National Natural Science Foundation of China(32171148,and 31770929)the Beijing Municipal Science and Technology Commission(Z181100001518001 and Z161100000216154).
文摘The oxytocin receptor(OXTR)has garnered increasing attention for its role in regulating both mature behaviors and brain development.It has been established that OXTR mediates a range of effects that are regionspecifc or period-specifc.However,the current studies of OXTR expression patterns in mice only provide limited help due to limitations in resolution.Therefore,our objective was to generate a comprehensive,high-resolution spatiotemporal expression map of Oxtr mRNA across the entire developing mouse brain.We applied RNAscope in situ hybridization to investigate the spatiotemporal expression pattern of Oxtr in the brains of male mice at six distinct postnatal developmental stages(P7,P14,P21,P28,P42,P56).We provide detailed descriptions of Oxtr expression patterns in key brain regions,including the cortex,basal forebrain,hippocampus,and amygdaloid complex,with a focus on the precise localization of Oxtr+cells and the variance of expression between diferent neurons.Furthermore,we identifed some neuronal populations with high Oxtr expression levels that have been little studied,including glutamatergic neurons in the ventral dentate gyrus,Vgat+Oxtr+cells in the basal forebrain,and GABAergic neurons in layers 4/5 of the cortex.Our study provides a novel perspective for understanding the distribution of Oxtr and encourages further investigations into its functions.
