【目的】旨在解析杜洛克猪剩余采食量的社会遗传效应(The social genetic effects of residual feed intake,SGE-RFI)的分子调控机制,揭示其通过能量代谢途径影响群体行为的生物学基础,为提高猪只饲料利用效率提供理论依据。【方法】通...【目的】旨在解析杜洛克猪剩余采食量的社会遗传效应(The social genetic effects of residual feed intake,SGE-RFI)的分子调控机制,揭示其通过能量代谢途径影响群体行为的生物学基础,为提高猪只饲料利用效率提供理论依据。【方法】通过RNA测序技术对杜洛克猪肝脏组织进行全转录组分析,系统筛选与SGE-RFI性状相关的关键差异表达基因,并采用功能富集方法解析其参与的生物学通路。【结果】鉴定出360个显著差异表达基因,功能分析表明这些基因主要富集于线粒体氧化磷酸化和ATP代谢等能量代谢相关通路,提示其可能通过调控机体能量稳态间接影响社会行为。载脂蛋白基因簇APOA1、APOC3和APOA4的协同表达改变,揭示了社会遗传效应调控过程中神经内分泌系统与脂代谢的交互作用。【结论】本研究构建了杜洛克猪SGE-RFI的多维度调控网络,不仅为解析群体行为遗传机制提供了新视角,更为精准选育低剩余采食量种猪和优化群体饲养管理方案奠定了分子基础。展开更多
Background:How AMP activated protein kinase(AMPK)signaling regulates mito-chondrial functions and mitophagy in human trophoblast cells remains unclear.This study was designed to investigate potential players mediating...Background:How AMP activated protein kinase(AMPK)signaling regulates mito-chondrial functions and mitophagy in human trophoblast cells remains unclear.This study was designed to investigate potential players mediating the regulation of AMPK on mitochondrial functions and mitophagy by next generation RNA-seq.Methods:We compared ATP production in protein kinase AMP-activated catalytic subunit alpha 1/2(PRKAA1/2)knockdown(AKD)and control BeWo cells using the Seahorse real-time ATP rate test,then analyzed gene expression profiling by RNA-seq.Differentially expressed genes(DEG)were examined by Gene Ontology(GO)analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment.Then protein-protein interactions(PPI)among mitochondria related genes were fur-ther analyzed using Metascape and Ingenuity Pathway Analysis(IPA)software.Results:Both mitochondrial and glycolytic ATP production in AKD cells were lower than in the control BeWo cells(CT),with a greater reduction of mitochondrial ATP production.A total of 1092 DEGs were identified,with 405 upregulated and 687 downregulated.GO analysis identified 60 genes associated with the term‘mitochon-drion’in the cellular component domain.PPI analysis identified three clusters of mito-chondria related genes,including aldo-keto reductase family 1 member B10 and B15(AKR1B10,AKR1B15),alanyl-tRNA synthetase 1(AARS1),mitochondrial ribosomal protein S6(MRPS6),mitochondrial calcium uniporter dominant negative subunit beta(MCUB)and dihydrolipoamide branched chain transacylase E2(DBT).Conclusions:In summary,this study identified multiple mitochondria related genes regulated by AMPK in BeWo cells,and among them,three clusters of genes may po-tentially contribute to altered mitochondrial functions in response to reduced AMPK signaling.展开更多
Bisphenol compounds(BPs)have various industrial uses and can enter the environment through various sources.To evaluate the ecotoxicity of BPs and identify potential gene candidates involved in the plant toxicity,Arabi...Bisphenol compounds(BPs)have various industrial uses and can enter the environment through various sources.To evaluate the ecotoxicity of BPs and identify potential gene candidates involved in the plant toxicity,Arabidopsis thaliana was exposed to bisphenol A(BPA),BPB,BPE,BPF,and BPS at 1,3,10 mg/L for a duration of 14 days,and their growth status were monitored.At day 14,roots and leaves were collected for internal BPs exposure concentration detection,RNA-seq(only roots),and morphological observations.As shown in the results,exposure to BPs significantly disturbed root elongation,exhibiting a trend of stimulation at low concentration and inhibition at high concentration.Additionally,BPs exhibited pronounced generation of reactive oxygen species,while none of the pollutants caused significant changes in root morphology.Internal exposure concentration analysis indicated that BPs tended to accumulate in the roots,with BPS exhibiting the highest level of accumulation.The results of RNA-seq indicated that the shared 211 differently expressed genes(DEGs)of these 5 exposure groups were enriched in defense response,generation of precursormetabolites,response to organic substance,response to oxygen-containing,response to hormone,oxidation-reduction process and so on.Regarding unique DEGs in each group,BPS wasmainly associated with the redox pathway,BPB primarily influenced seed germination,and BPA,BPE and BPF were primarily involved in metabolic signaling pathways.Our results provide newinsights for BPs induced adverse effects on Arabidopsis thaliana and suggest that the ecological risks associated with BPA alternatives cannot be ignored.展开更多
文摘【目的】旨在解析杜洛克猪剩余采食量的社会遗传效应(The social genetic effects of residual feed intake,SGE-RFI)的分子调控机制,揭示其通过能量代谢途径影响群体行为的生物学基础,为提高猪只饲料利用效率提供理论依据。【方法】通过RNA测序技术对杜洛克猪肝脏组织进行全转录组分析,系统筛选与SGE-RFI性状相关的关键差异表达基因,并采用功能富集方法解析其参与的生物学通路。【结果】鉴定出360个显著差异表达基因,功能分析表明这些基因主要富集于线粒体氧化磷酸化和ATP代谢等能量代谢相关通路,提示其可能通过调控机体能量稳态间接影响社会行为。载脂蛋白基因簇APOA1、APOC3和APOA4的协同表达改变,揭示了社会遗传效应调控过程中神经内分泌系统与脂代谢的交互作用。【结论】本研究构建了杜洛克猪SGE-RFI的多维度调控网络,不仅为解析群体行为遗传机制提供了新视角,更为精准选育低剩余采食量种猪和优化群体饲养管理方案奠定了分子基础。
基金Dean's Office Howard University College of Medicine,Grant/Award Number:Bridge Fund/Pilot Study AwardNational Center on Minority Health and Health Disparities,Grant/Award Number:RCMI/IDC Award U54MD007597National Institute of Child Health and Human Development,Grant/Award Number:R03HD095417 and R16HD116702。
文摘Background:How AMP activated protein kinase(AMPK)signaling regulates mito-chondrial functions and mitophagy in human trophoblast cells remains unclear.This study was designed to investigate potential players mediating the regulation of AMPK on mitochondrial functions and mitophagy by next generation RNA-seq.Methods:We compared ATP production in protein kinase AMP-activated catalytic subunit alpha 1/2(PRKAA1/2)knockdown(AKD)and control BeWo cells using the Seahorse real-time ATP rate test,then analyzed gene expression profiling by RNA-seq.Differentially expressed genes(DEG)were examined by Gene Ontology(GO)analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment.Then protein-protein interactions(PPI)among mitochondria related genes were fur-ther analyzed using Metascape and Ingenuity Pathway Analysis(IPA)software.Results:Both mitochondrial and glycolytic ATP production in AKD cells were lower than in the control BeWo cells(CT),with a greater reduction of mitochondrial ATP production.A total of 1092 DEGs were identified,with 405 upregulated and 687 downregulated.GO analysis identified 60 genes associated with the term‘mitochon-drion’in the cellular component domain.PPI analysis identified three clusters of mito-chondria related genes,including aldo-keto reductase family 1 member B10 and B15(AKR1B10,AKR1B15),alanyl-tRNA synthetase 1(AARS1),mitochondrial ribosomal protein S6(MRPS6),mitochondrial calcium uniporter dominant negative subunit beta(MCUB)and dihydrolipoamide branched chain transacylase E2(DBT).Conclusions:In summary,this study identified multiple mitochondria related genes regulated by AMPK in BeWo cells,and among them,three clusters of genes may po-tentially contribute to altered mitochondrial functions in response to reduced AMPK signaling.
基金supported by the National Science Foundation of China (No.22106098)the Youth Science and Technology Research Foundation of Shanxi Province (No.20210302124298)+2 种基金the Scientific and Technological Innovation Programs of Higher Education Institutions in Shanxi (Nos.2020L0174,and 2020L0025)the Startup Foundation for Doctors of Shanxi Province (No.SD1917)the Startup Foundation for Doctors of Shanxi Medical University (No.XD1917).
文摘Bisphenol compounds(BPs)have various industrial uses and can enter the environment through various sources.To evaluate the ecotoxicity of BPs and identify potential gene candidates involved in the plant toxicity,Arabidopsis thaliana was exposed to bisphenol A(BPA),BPB,BPE,BPF,and BPS at 1,3,10 mg/L for a duration of 14 days,and their growth status were monitored.At day 14,roots and leaves were collected for internal BPs exposure concentration detection,RNA-seq(only roots),and morphological observations.As shown in the results,exposure to BPs significantly disturbed root elongation,exhibiting a trend of stimulation at low concentration and inhibition at high concentration.Additionally,BPs exhibited pronounced generation of reactive oxygen species,while none of the pollutants caused significant changes in root morphology.Internal exposure concentration analysis indicated that BPs tended to accumulate in the roots,with BPS exhibiting the highest level of accumulation.The results of RNA-seq indicated that the shared 211 differently expressed genes(DEGs)of these 5 exposure groups were enriched in defense response,generation of precursormetabolites,response to organic substance,response to oxygen-containing,response to hormone,oxidation-reduction process and so on.Regarding unique DEGs in each group,BPS wasmainly associated with the redox pathway,BPB primarily influenced seed germination,and BPA,BPE and BPF were primarily involved in metabolic signaling pathways.Our results provide newinsights for BPs induced adverse effects on Arabidopsis thaliana and suggest that the ecological risks associated with BPA alternatives cannot be ignored.
