类受体激酶(receptor like kinase,RLK)在植物生长发育和环境适应中起重要作用。前期研究发现苹果(Malus′domestica)中存在一类具有Glyco18胞外结构域(SM000636或SM000704)的RLK,命名为Glyco_18-RLK(Gly-RLK)。本试验中以Glyco18和Pkin...类受体激酶(receptor like kinase,RLK)在植物生长发育和环境适应中起重要作用。前期研究发现苹果(Malus′domestica)中存在一类具有Glyco18胞外结构域(SM000636或SM000704)的RLK,命名为Glyco_18-RLK(Gly-RLK)。本试验中以Glyco18和Pkinase(SM000220)保守域全蛋白序列为种子序列,对53种被子植物基因组中的Gly-RLK进行鉴定,并分析其进化特征;明确苹果Gly-RLK(MdGly-RLK)的理化性状、亚细胞定位,基因在染色体上的分布、启动子区域的顺式作用元件和表达模式。结果表明,仅19种植物中存在Gly-RLK,家族成员数介于1~20,单子叶植物中均未发现该家族成员。根据进化分析将其分为8个亚组,亚组Ⅲ、Ⅶ和Ⅷ分布基因数较多。共发现6个MdGly-RLK,其编码的氨基酸序列大小、分子量和等电点分别介于451~776、50.99~87.33 kD和5.95~8.21,主要位于质膜,4个为串联重复。MdGly-RLK在苹果各组织和品种间存在不同的表达模式;接种苹果腐烂病菌(Valsa mali)后,6个MdGly-RLK均差异表达,MdGly-RLK6(MD15G1156900)可上调至对照的16.33倍。综上,Gly-RLK仅在部分双子叶植物中存在,家族成员的扩增速度较快。MdGly-RLK响应苹果生长发育和黑腐皮壳菌信号,MdGly-RLK6可作为苹果抗腐烂病研究的候选基因。展开更多
Receptor-like kinases （RLKs） play crucial roles in cellular signal perception and propagation. To study the evolutionary relationships among RLKs in soybean, a large-scale expressed sequence tags （ESTs） survey for...Receptor-like kinases （RLKs） play crucial roles in cellular signal perception and propagation. To study the evolutionary relationships among RLKs in soybean, a large-scale expressed sequence tags （ESTs） survey for RLKs-related sequences was conducted. By doing BLAST analysis using our database and The Gene Index Database, 605 putative RLK genes were identified. Based on the phylogeny of the kinase domain, these soybean RLKs were classified into 58 different small subfamilies. The phylogenetic analysis of RLKs in soybean, rice and Arabidopsis showed that different subfamilies of RLKs had different functions and could have experienced different selective pressures.展开更多
As a barrier between the cell and its environment,the plant cell wall provides structural support during development and stress response.Plants are able to sense their surroundings and adjust their activities accordin...As a barrier between the cell and its environment,the plant cell wall provides structural support during development and stress response.Plants are able to sense their surroundings and adjust their activities accordingly.A crucial mechanism involved in these adaptive changes is the cell wall integrity(CWI)maintenance mechanism,which monitors and maintains the integrity of cell walls via changes in cell and cell wall metabolism without destroying cell wall organization.Different abiotic stresses and changes in plant developmental phases disrupt CWI.However,emerging evidence has demonstrated the initiation of CWI signaling mechanisms as key in promoting plant growth in complex situations.This review discusses recent advances in the Catharanthus roseus receptor-like kinase 1-like(CrRLK1L)protein function in plant cell wall signaling during adaptation to changing environments and development.We conclude by highlighting how current spatially resolved transcriptomics may be used to advance the role of CrRLK1L members in plant cell wall signaling during development and stress response.展开更多
Different from animals, sessile plants are equipped with a large receptor-like kinase(RLK) superfamily. RLKs are a family of single trans-membrane proteins with divergent N-terminal extracellular domains capped by a s...Different from animals, sessile plants are equipped with a large receptor-like kinase(RLK) superfamily. RLKs are a family of single trans-membrane proteins with divergent N-terminal extracellular domains capped by a signal peptide and C-terminal intracellular kinase. Researches in the last two decades have uncovered an increasing number of RLKs that regulate plant development, stress response and sexual reproduction, highlighting a dominant role of RLK signaling in cell-to-cell communications. Sexual reproduction in flowering plants is featured by interactions between the male gametophyte and the female tissues to facilitate sperm delivery and fertilization. Emerging evidences suggest that RLKs regulate almost every aspect of plant reproductive process, especially during pollination. Therefore, in this review we will focus mainly on the function and signaling of RLKs in plant male-female interaction and discuss the future prospects on these topics.展开更多
在广谱抗稻瘟病品种云引与普感稻瘟病品种丽江新团黑谷(Lijiangxintuanheigu,LTH)构建的近等基因系抗、感病子代基因组重测序的差异基因中,从与免疫应答(immune response)相关的分类单元里得到8个基因序列有差异的基因.它们都为编码富...在广谱抗稻瘟病品种云引与普感稻瘟病品种丽江新团黑谷(Lijiangxintuanheigu,LTH)构建的近等基因系抗、感病子代基因组重测序的差异基因中,从与免疫应答(immune response)相关的分类单元里得到8个基因序列有差异的基因.它们都为编码富含亮氨酸重复序列的类受体蛋白激酶基因,并聚集于11号染色体的邻近位置,且与已知的白叶枯病抗性基因Xa21具有一定同源性.为进一步探究这些类受体蛋白激酶类基因在稻瘟病抗性中可能发挥的功能,我们分析了这些基因的结构、进化及抗感子代间的序列差异类型,同时还分别采用离体和喷雾接菌的方式对云引和LTH进行稻瘟病接种并取样.通过实时荧光定量PCR(quantitative real time polymerase chain reaction,q RT-PCR)发现,部分基因能受到稻瘟病菌的诱导,推测它们可能在水稻对抗稻瘟病病菌中发挥一定作用,为进一步挖掘稻瘟病相关抗性基因奠定基础.展开更多
文摘类受体激酶(receptor like kinase,RLK)在植物生长发育和环境适应中起重要作用。前期研究发现苹果(Malus′domestica)中存在一类具有Glyco18胞外结构域(SM000636或SM000704)的RLK,命名为Glyco_18-RLK(Gly-RLK)。本试验中以Glyco18和Pkinase(SM000220)保守域全蛋白序列为种子序列,对53种被子植物基因组中的Gly-RLK进行鉴定,并分析其进化特征;明确苹果Gly-RLK(MdGly-RLK)的理化性状、亚细胞定位,基因在染色体上的分布、启动子区域的顺式作用元件和表达模式。结果表明,仅19种植物中存在Gly-RLK,家族成员数介于1~20,单子叶植物中均未发现该家族成员。根据进化分析将其分为8个亚组,亚组Ⅲ、Ⅶ和Ⅷ分布基因数较多。共发现6个MdGly-RLK,其编码的氨基酸序列大小、分子量和等电点分别介于451~776、50.99~87.33 kD和5.95~8.21,主要位于质膜,4个为串联重复。MdGly-RLK在苹果各组织和品种间存在不同的表达模式;接种苹果腐烂病菌(Valsa mali)后,6个MdGly-RLK均差异表达,MdGly-RLK6(MD15G1156900)可上调至对照的16.33倍。综上,Gly-RLK仅在部分双子叶植物中存在,家族成员的扩增速度较快。MdGly-RLK响应苹果生长发育和黑腐皮壳菌信号,MdGly-RLK6可作为苹果抗腐烂病研究的候选基因。
基金supported by the National Basic Research Program of China (No. 2004CB117200)the National High-Tech Project (No. 2006AA10Z18201)
文摘Receptor-like kinases （RLKs） play crucial roles in cellular signal perception and propagation. To study the evolutionary relationships among RLKs in soybean, a large-scale expressed sequence tags （ESTs） survey for RLKs-related sequences was conducted. By doing BLAST analysis using our database and The Gene Index Database, 605 putative RLK genes were identified. Based on the phylogeny of the kinase domain, these soybean RLKs were classified into 58 different small subfamilies. The phylogenetic analysis of RLKs in soybean, rice and Arabidopsis showed that different subfamilies of RLKs had different functions and could have experienced different selective pressures.
基金supported by the National Key Research and Development Plan of China(2021YFF1001100)National Natural Science Foundation of China(32341033)the Key Research and Development Plan of Heilongjiang Province(GZ2024ZX02B13).
文摘As a barrier between the cell and its environment,the plant cell wall provides structural support during development and stress response.Plants are able to sense their surroundings and adjust their activities accordingly.A crucial mechanism involved in these adaptive changes is the cell wall integrity(CWI)maintenance mechanism,which monitors and maintains the integrity of cell walls via changes in cell and cell wall metabolism without destroying cell wall organization.Different abiotic stresses and changes in plant developmental phases disrupt CWI.However,emerging evidence has demonstrated the initiation of CWI signaling mechanisms as key in promoting plant growth in complex situations.This review discusses recent advances in the Catharanthus roseus receptor-like kinase 1-like(CrRLK1L)protein function in plant cell wall signaling during adaptation to changing environments and development.We conclude by highlighting how current spatially resolved transcriptomics may be used to advance the role of CrRLK1L members in plant cell wall signaling during development and stress response.
基金supported by the Ministry of Science and Technology of China(2013CB945103)the National Natural Science Foundation of China(31330053)
文摘Different from animals, sessile plants are equipped with a large receptor-like kinase(RLK) superfamily. RLKs are a family of single trans-membrane proteins with divergent N-terminal extracellular domains capped by a signal peptide and C-terminal intracellular kinase. Researches in the last two decades have uncovered an increasing number of RLKs that regulate plant development, stress response and sexual reproduction, highlighting a dominant role of RLK signaling in cell-to-cell communications. Sexual reproduction in flowering plants is featured by interactions between the male gametophyte and the female tissues to facilitate sperm delivery and fertilization. Emerging evidences suggest that RLKs regulate almost every aspect of plant reproductive process, especially during pollination. Therefore, in this review we will focus mainly on the function and signaling of RLKs in plant male-female interaction and discuss the future prospects on these topics.
文摘在广谱抗稻瘟病品种云引与普感稻瘟病品种丽江新团黑谷(Lijiangxintuanheigu,LTH)构建的近等基因系抗、感病子代基因组重测序的差异基因中,从与免疫应答(immune response)相关的分类单元里得到8个基因序列有差异的基因.它们都为编码富含亮氨酸重复序列的类受体蛋白激酶基因,并聚集于11号染色体的邻近位置,且与已知的白叶枯病抗性基因Xa21具有一定同源性.为进一步探究这些类受体蛋白激酶类基因在稻瘟病抗性中可能发挥的功能,我们分析了这些基因的结构、进化及抗感子代间的序列差异类型,同时还分别采用离体和喷雾接菌的方式对云引和LTH进行稻瘟病接种并取样.通过实时荧光定量PCR(quantitative real time polymerase chain reaction,q RT-PCR)发现,部分基因能受到稻瘟病菌的诱导,推测它们可能在水稻对抗稻瘟病病菌中发挥一定作用,为进一步挖掘稻瘟病相关抗性基因奠定基础.
