为了研究引起水稻叶片卷曲的分子机理,鉴定出新的水稻卷叶基因.用60Co-γ射线辐射诱变籼稻品种镇恢084,获得一份卷叶矮化突变体材料,命名为rld(rolling leaf and dwarf).通过形态学分析水稻表型,石蜡切片观察叶片细胞组织形态,图位克隆...为了研究引起水稻叶片卷曲的分子机理,鉴定出新的水稻卷叶基因.用60Co-γ射线辐射诱变籼稻品种镇恢084,获得一份卷叶矮化突变体材料,命名为rld(rolling leaf and dwarf).通过形态学分析水稻表型,石蜡切片观察叶片细胞组织形态,图位克隆和测序技术进行精细定位和确定目的基因,生物信息学分析蛋白序列结构.结果显示:rld突变体叶片极度内卷,株高降低,穗长变短,结实率降低;rld突变体叶片维管束间的下表皮叶肉细胞面积增大;rld基因精细定位在标记Indel2和Indel5间的32.3kb的物理区间,测序发现rld是调控卷叶基因RL9的一个新等位基因,由于外显子上精氨酸缺失引起rld基因编码的蛋白空间结构发生改变.推测精氨酸在RL9蛋白的正常功能行使过程中是必要的,对维持水稻叶片表型具有至关重要的作用.展开更多
Ensuring the secure transmission of secret messages,particularly through video—one of the most widely used media formats—is a critical challenge in the field of information security.Relying on a single-layered secur...Ensuring the secure transmission of secret messages,particularly through video—one of the most widely used media formats—is a critical challenge in the field of information security.Relying on a single-layered security approach is often insufficient for safeguarding sensitive data.This study proposes a triple-lightweight cryptographic and steganographic model that integrates the Hill Cipher Technique(HCT),Rotation Left Digits(RLD),and Discrete Wavelet Transform(DWT)to embed secret messages within video frames securely.The approach begins with encrypting the secret text using a private key matrix(PK^(1))of size 2×2 up to 6×6 via HCT.A second encryption layer is applied using a dynamic private key(PK2)derived from the RGB pixel values of the video frame,resulting in a rotated cipher.The doubly encrypted message is then embedded into the video frames using the DWT method.Upon transmission,the concealed message is extracted using inverse DWT and decrypted in two steps—first with PK2 and then with the inverse of PK^(1).Experiments conducted using MPEG video sequences and message lengths ranging from 10 to 300 bytes demonstrate strong performance in terms of Mean Square Error(MSE),Peak Signal-to-Noise Ratio(PSNR),and Correlation Coefficient(CC)between original and encrypted messages.The similarity between original and stego frames is further validated using Structural Similarity Index(SSIM),Mean Absolute Error(MAE),Number of Pixel Change Rate(NPCR),and Unified Average Changing Intensity(UACI).Results confirm that utilizing video frames to generate PK2 offers superior security compared to static key images.Moreover,the indistinguishability between original and stego frames highlights the method’s robustness against visual and statistical attacks.展开更多
目的:探讨E3泛素连接酶HERC6(hect and RLD domain containing E3 ubiquitin protein ligase family member 6)是否可作为肾透明细胞肾细胞癌(kidney clear cell renal cell carcinoma,KIRC)预后的标志物和治疗的靶点。方法:用癌症基因...目的:探讨E3泛素连接酶HERC6(hect and RLD domain containing E3 ubiquitin protein ligase family member 6)是否可作为肾透明细胞肾细胞癌(kidney clear cell renal cell carcinoma,KIRC)预后的标志物和治疗的靶点。方法:用癌症基因图谱(the cancer genome atlas,TCGA)数据库分析在KIRC中HERC6的表达。运用UALCAN数据库评估HERC6与KIRC的临床相关性。进行单因素Cox回归分析,构建HERC6在KIRC的预后模型。探究HERC6表达与免疫细胞浸润、免疫相关功能以及免疫检查点之间的关联性。通过药物敏感性分析探究HERC6作为靶向治疗新靶点的可能。最后,开展5-乙炔基-2'脱氧尿嘧啶掺入测定(5-ethynyl-2'-deoxyuridine incorporation assay,EdU)实验、划痕实验和Transwell实验,以验证HERC6在KIRC发生、进展中的作用。结果:HERC6在KIRC中的表达与正常肾组织相比明显减低(P<0.001)。HERC6低表达的患者预后较差,临床病理特征也更不理想。功能富集分析显示,HERC6主要参与免疫反应、脂质代谢等生物学过程,进而介导KIRC的发展。免疫相关分析表明,HERC6是KIRC免疫治疗的潜在靶点。在KIRC细胞中过表达HERC6进行体外实验,发现其增殖、迁移和侵袭能力被抑制。结论:HERC6可作为KIRC预后标志物和治疗靶点。展开更多
文摘为了研究引起水稻叶片卷曲的分子机理,鉴定出新的水稻卷叶基因.用60Co-γ射线辐射诱变籼稻品种镇恢084,获得一份卷叶矮化突变体材料,命名为rld(rolling leaf and dwarf).通过形态学分析水稻表型,石蜡切片观察叶片细胞组织形态,图位克隆和测序技术进行精细定位和确定目的基因,生物信息学分析蛋白序列结构.结果显示:rld突变体叶片极度内卷,株高降低,穗长变短,结实率降低;rld突变体叶片维管束间的下表皮叶肉细胞面积增大;rld基因精细定位在标记Indel2和Indel5间的32.3kb的物理区间,测序发现rld是调控卷叶基因RL9的一个新等位基因,由于外显子上精氨酸缺失引起rld基因编码的蛋白空间结构发生改变.推测精氨酸在RL9蛋白的正常功能行使过程中是必要的,对维持水稻叶片表型具有至关重要的作用.
文摘Ensuring the secure transmission of secret messages,particularly through video—one of the most widely used media formats—is a critical challenge in the field of information security.Relying on a single-layered security approach is often insufficient for safeguarding sensitive data.This study proposes a triple-lightweight cryptographic and steganographic model that integrates the Hill Cipher Technique(HCT),Rotation Left Digits(RLD),and Discrete Wavelet Transform(DWT)to embed secret messages within video frames securely.The approach begins with encrypting the secret text using a private key matrix(PK^(1))of size 2×2 up to 6×6 via HCT.A second encryption layer is applied using a dynamic private key(PK2)derived from the RGB pixel values of the video frame,resulting in a rotated cipher.The doubly encrypted message is then embedded into the video frames using the DWT method.Upon transmission,the concealed message is extracted using inverse DWT and decrypted in two steps—first with PK2 and then with the inverse of PK^(1).Experiments conducted using MPEG video sequences and message lengths ranging from 10 to 300 bytes demonstrate strong performance in terms of Mean Square Error(MSE),Peak Signal-to-Noise Ratio(PSNR),and Correlation Coefficient(CC)between original and encrypted messages.The similarity between original and stego frames is further validated using Structural Similarity Index(SSIM),Mean Absolute Error(MAE),Number of Pixel Change Rate(NPCR),and Unified Average Changing Intensity(UACI).Results confirm that utilizing video frames to generate PK2 offers superior security compared to static key images.Moreover,the indistinguishability between original and stego frames highlights the method’s robustness against visual and statistical attacks.
文摘目的:探讨E3泛素连接酶HERC6(hect and RLD domain containing E3 ubiquitin protein ligase family member 6)是否可作为肾透明细胞肾细胞癌(kidney clear cell renal cell carcinoma,KIRC)预后的标志物和治疗的靶点。方法:用癌症基因图谱(the cancer genome atlas,TCGA)数据库分析在KIRC中HERC6的表达。运用UALCAN数据库评估HERC6与KIRC的临床相关性。进行单因素Cox回归分析,构建HERC6在KIRC的预后模型。探究HERC6表达与免疫细胞浸润、免疫相关功能以及免疫检查点之间的关联性。通过药物敏感性分析探究HERC6作为靶向治疗新靶点的可能。最后,开展5-乙炔基-2'脱氧尿嘧啶掺入测定(5-ethynyl-2'-deoxyuridine incorporation assay,EdU)实验、划痕实验和Transwell实验,以验证HERC6在KIRC发生、进展中的作用。结果:HERC6在KIRC中的表达与正常肾组织相比明显减低(P<0.001)。HERC6低表达的患者预后较差,临床病理特征也更不理想。功能富集分析显示,HERC6主要参与免疫反应、脂质代谢等生物学过程,进而介导KIRC的发展。免疫相关分析表明,HERC6是KIRC免疫治疗的潜在靶点。在KIRC细胞中过表达HERC6进行体外实验,发现其增殖、迁移和侵袭能力被抑制。结论:HERC6可作为KIRC预后标志物和治疗靶点。
