得益于基因测序技术的快速发展,基因组测序数据呈现爆炸式增长,核糖体合成和翻译后修饰肽(RiPPs)是近十年逐渐进入人们视野的一大类肽类天然产物。这类化合物在自然界中分布极其广泛,具有丰富的结构多样性和生物活性多样性,是天然药物...得益于基因测序技术的快速发展,基因组测序数据呈现爆炸式增长,核糖体合成和翻译后修饰肽(RiPPs)是近十年逐渐进入人们视野的一大类肽类天然产物。这类化合物在自然界中分布极其广泛,具有丰富的结构多样性和生物活性多样性,是天然药物的重要来源。RiPPs的发现主要依赖低通量生物实验,传统方法精确但成本高昂,随着新型计算机技术的更新迭代,包括antiSMASH、RiPP-PRISM等在内的生物信息学工具能够极大加速RiPPs挖掘进程,但依然无法突破基于同源性方法(例如搜索保守的生物合成酶)的限制——无法有效识别具有不同生物合成机制的新型RiPPs。在这里,本文首次基于自然语言处理预训练模型BERT,提出四种可以完全依赖序列数据识别RiPPs而非基于同源性及基因组上下文信息的深度学习模型,通过对各模型进行验证分析和对比,最终确定在RiPPs识别赛道上表现卓越的最佳模型BERiPPs(bidirectional language model for enhancing the performance of identification of RiPPs precursor peptides)。BERiPPs能够在不考虑基因组背景的情况下以无偏见的方式识别RiPPs前体肽,并可通过条件随机场生成对前导肽裂解位点的预测,为高通量挖掘全新RiPPs提供了思路,并在一定程度下揭示了前体肽和修饰酶间的生物学底层关系。展开更多
To meet the demands for high octane gasoline and aromatics, catalytic reforming process has been advancing quickly in China. The reforming catalysts developed by RIPP have been used in more than 80% capacity of domest...To meet the demands for high octane gasoline and aromatics, catalytic reforming process has been advancing quickly in China. The reforming catalysts developed by RIPP have been used in more than 80% capacity of domestic CCR and SR units. This paper introduces the properties of PS VI CCR catalyst developed by RIPP in recent years and also the result from commercial units. The PS VI catalyst has high activity and good selectivity, under the same reaction conditions, the carbon on catalyst was lowered by 26% in mass as compared with that of the reference catalyst. Among the SR reforming catalysts, the new type of PRT series catalysts have excellent performance at low reaction pressure compared with the ref. Cat A. The aromatics and reformate mass yields of PRT catalyst were 2%～3% and 3%, respectively, higher than those of Cat A, and the run length was 30%～40% longer as well, which exhibits good prospect of application.展开更多
On July 2, 2018 the commercial test unit for selective polymerization of C_4 olefins jointly developed by the SINOPEC Research Institute of Petroleum Processing (RIPP) and the SINOPEC Shijiazhuang Refining and Chemica...On July 2, 2018 the commercial test unit for selective polymerization of C_4 olefins jointly developed by the SINOPEC Research Institute of Petroleum Processing (RIPP) and the SINOPEC Shijiazhuang Refining and Chemical Branch Company in collaboration with other institutions had been successfully put on stream展开更多
In April 2018 the SINOPEC Corporation had launched its "green enterprise action program" by proposing to set up the clean, highly efficient, low-carbon, and recyclable green enterprises under its jurisdictio...In April 2018 the SINOPEC Corporation had launched its "green enterprise action program" by proposing to set up the clean, highly efficient, low-carbon, and recyclable green enterprises under its jurisdiction by 2023.All SINOPEC’s catalyst factories are confronted with double pressures demanding an improved green produc-展开更多
Recently the sulfuric acid alkylation process, jointly developed by the SINOPEC Research Institute of Petroleum Processing (RIPP) and the SINOPEC Shijiazhuang Refining and Chemical Branch Company,has been delivering q...Recently the sulfuric acid alkylation process, jointly developed by the SINOPEC Research Institute of Petroleum Processing (RIPP) and the SINOPEC Shijiazhuang Refining and Chemical Branch Company,has been delivering qualified product from the 200展开更多
In March 2012 after competing with several internationalmajor oil companies on the same arena the SINOPEC ResearchInstitute of Petroleum Processing （RIPP）, proppedup by the outstanding performance and good commercia...In March 2012 after competing with several internationalmajor oil companies on the same arena the SINOPEC ResearchInstitute of Petroleum Processing （RIPP）, proppedup by the outstanding performance and good commercialapplication records of the third-generation RHT seriesresid hydrotreating catalysts, had successfully won thebid for the 19th cycle for application of the hydrotreatingcatalyst in the 1.5 Mt/a RDS unit at Taiwan CPC Corporation＇sDalin展开更多
Recently the ASTM standard test method (ASTM D8144-18)―"The standard test method for separation and determination of aromatics, non-aromatics and fatty acid methyl ester (FAME) fractions in middle distillates by...Recently the ASTM standard test method (ASTM D8144-18)―"The standard test method for separation and determination of aromatics, non-aromatics and fatty acid methyl ester (FAME) fractions in middle distillates by solid phase extraction andgas chromatography"―drafted and drawn up by the SINOPEC Research Institute of Petroleum Processing (RIPP), has been展开更多
In April 2018 the Sinopec Corporation launched "The Green Enterprises Action Program" by proposing to construct all clean,high-efficiency,low-carbon and recyclable green enterprises by 2023.The catalyst fact...In April 2018 the Sinopec Corporation launched "The Green Enterprises Action Program" by proposing to construct all clean,high-efficiency,low-carbon and recyclable green enterprises by 2023.The catalyst factories under the Sinopec Corporation are faced with the double pressure on enhancing the green production capability展开更多
Currently the project'Development and commercia application of FCC catalyst for boosting gasoline yield'jointly undertaken by the SINOPEC Research Institute of Petroleum Processing(RIPP),the SINOPEC Yanshan Br...Currently the project'Development and commercia application of FCC catalyst for boosting gasoline yield'jointly undertaken by the SINOPEC Research Institute of Petroleum Processing(RIPP),the SINOPEC Yanshan Branch Co.and the Branch of SINOPEC Catalyst Company has passed the appraisal.The catalyst aimed at boosting展开更多
Some plants use intracellular immune receptors,mainly from the nucleotide-binding leucine-rich repeat receptors(NLRs)super-family,to detect pathogen effectors and restrict pathogen infection.NLRs are categorized as TI...Some plants use intracellular immune receptors,mainly from the nucleotide-binding leucine-rich repeat receptors(NLRs)super-family,to detect pathogen effectors and restrict pathogen infection.NLRs are categorized as TIR-NLR(TNL)or CC-NLR(CNL)based on their N-terminal Tollinterleukin 1-like receptor(TIR)or coiled-coil(CC)domain.Additionally,there are helper(h)NLRs that aid in translating signals from pathogen-sensing NLRs into effector-triggered immunity responses.NLRs can directly detect effectors or indirectly monitor their host targets,leading to a robust immune response and programmed cell death,known as the hypersensitive response(HR).展开更多
Ribosomally synthesized and post-translationally modified peptides(RiPPs)are a class of cyclic or linear peptidic natural products with remarkable structural and functional diversity.Recent advances in genomics and sy...Ribosomally synthesized and post-translationally modified peptides(RiPPs)are a class of cyclic or linear peptidic natural products with remarkable structural and functional diversity.Recent advances in genomics and synthetic biology,are facilitating us to discover a large number of new ribosomal natural products,including lanthipeptides,lasso peptides,sactipeptides,thiopeptides,microviridins,cyanobactins,linear thiazole/oxazole-containing peptides and so on.In this review,we summarize bioinformatic strategies that have been developed to identify and prioritize biosynthetic gene clusters(BGCs)encoding RiPPs,and the genome mining-guided discovery of novel RiPPs.We also prospectively provide a vision of what genomics-guided discovery of RiPPs may look like in the future,especially the discovery of RiPPs from dominant but uncultivated microbes,which will be promoted by the combinational use of synthetic biology and metagenome mining strategies.展开更多
文摘得益于基因测序技术的快速发展,基因组测序数据呈现爆炸式增长,核糖体合成和翻译后修饰肽(RiPPs)是近十年逐渐进入人们视野的一大类肽类天然产物。这类化合物在自然界中分布极其广泛,具有丰富的结构多样性和生物活性多样性,是天然药物的重要来源。RiPPs的发现主要依赖低通量生物实验,传统方法精确但成本高昂,随着新型计算机技术的更新迭代,包括antiSMASH、RiPP-PRISM等在内的生物信息学工具能够极大加速RiPPs挖掘进程,但依然无法突破基于同源性方法(例如搜索保守的生物合成酶)的限制——无法有效识别具有不同生物合成机制的新型RiPPs。在这里,本文首次基于自然语言处理预训练模型BERT,提出四种可以完全依赖序列数据识别RiPPs而非基于同源性及基因组上下文信息的深度学习模型,通过对各模型进行验证分析和对比,最终确定在RiPPs识别赛道上表现卓越的最佳模型BERiPPs(bidirectional language model for enhancing the performance of identification of RiPPs precursor peptides)。BERiPPs能够在不考虑基因组背景的情况下以无偏见的方式识别RiPPs前体肽,并可通过条件随机场生成对前导肽裂解位点的预测,为高通量挖掘全新RiPPs提供了思路,并在一定程度下揭示了前体肽和修饰酶间的生物学底层关系。
文摘To meet the demands for high octane gasoline and aromatics, catalytic reforming process has been advancing quickly in China. The reforming catalysts developed by RIPP have been used in more than 80% capacity of domestic CCR and SR units. This paper introduces the properties of PS VI CCR catalyst developed by RIPP in recent years and also the result from commercial units. The PS VI catalyst has high activity and good selectivity, under the same reaction conditions, the carbon on catalyst was lowered by 26% in mass as compared with that of the reference catalyst. Among the SR reforming catalysts, the new type of PRT series catalysts have excellent performance at low reaction pressure compared with the ref. Cat A. The aromatics and reformate mass yields of PRT catalyst were 2%～3% and 3%, respectively, higher than those of Cat A, and the run length was 30%～40% longer as well, which exhibits good prospect of application.
文摘On July 2, 2018 the commercial test unit for selective polymerization of C_4 olefins jointly developed by the SINOPEC Research Institute of Petroleum Processing (RIPP) and the SINOPEC Shijiazhuang Refining and Chemical Branch Company in collaboration with other institutions had been successfully put on stream
文摘In April 2018 the SINOPEC Corporation had launched its "green enterprise action program" by proposing to set up the clean, highly efficient, low-carbon, and recyclable green enterprises under its jurisdiction by 2023.All SINOPEC’s catalyst factories are confronted with double pressures demanding an improved green produc-
文摘Recently the sulfuric acid alkylation process, jointly developed by the SINOPEC Research Institute of Petroleum Processing (RIPP) and the SINOPEC Shijiazhuang Refining and Chemical Branch Company,has been delivering qualified product from the 200
文摘In March 2012 after competing with several internationalmajor oil companies on the same arena the SINOPEC ResearchInstitute of Petroleum Processing （RIPP）, proppedup by the outstanding performance and good commercialapplication records of the third-generation RHT seriesresid hydrotreating catalysts, had successfully won thebid for the 19th cycle for application of the hydrotreatingcatalyst in the 1.5 Mt/a RDS unit at Taiwan CPC Corporation＇sDalin
文摘Recently the ASTM standard test method (ASTM D8144-18)―"The standard test method for separation and determination of aromatics, non-aromatics and fatty acid methyl ester (FAME) fractions in middle distillates by solid phase extraction andgas chromatography"―drafted and drawn up by the SINOPEC Research Institute of Petroleum Processing (RIPP), has been
文摘In April 2018 the Sinopec Corporation launched "The Green Enterprises Action Program" by proposing to construct all clean,high-efficiency,low-carbon and recyclable green enterprises by 2023.The catalyst factories under the Sinopec Corporation are faced with the double pressure on enhancing the green production capability
文摘Currently the project'Development and commercia application of FCC catalyst for boosting gasoline yield'jointly undertaken by the SINOPEC Research Institute of Petroleum Processing(RIPP),the SINOPEC Yanshan Branch Co.and the Branch of SINOPEC Catalyst Company has passed the appraisal.The catalyst aimed at boosting
基金supported by grants from the National Natural Science Foundation of China(32372483,32302296,32272641)the Fundamental Research Funds for the Central Universities(GK202201017)。
文摘Some plants use intracellular immune receptors,mainly from the nucleotide-binding leucine-rich repeat receptors(NLRs)super-family,to detect pathogen effectors and restrict pathogen infection.NLRs are categorized as TIR-NLR(TNL)or CC-NLR(CNL)based on their N-terminal Tollinterleukin 1-like receptor(TIR)or coiled-coil(CC)domain.Additionally,there are helper(h)NLRs that aid in translating signals from pathogen-sensing NLRs into effector-triggered immunity responses.NLRs can directly detect effectors or indirectly monitor their host targets,leading to a robust immune response and programmed cell death,known as the hypersensitive response(HR).
基金Z.Zhong,B.He,and Y-X Li acknowledge a partial financial support from the National Key R&D Program of China(2018YFA0903200)Southern Marine Science and Engineering Guangdong Laboratory(Guangzhou)(SMSEGL20SC01)J.Li acknowledges a partial financial support by a National Institutes of Health(NIH)grant P20GM103641 and a National Science Foundation EPSCoR Program OIA-1655740.
文摘Ribosomally synthesized and post-translationally modified peptides(RiPPs)are a class of cyclic or linear peptidic natural products with remarkable structural and functional diversity.Recent advances in genomics and synthetic biology,are facilitating us to discover a large number of new ribosomal natural products,including lanthipeptides,lasso peptides,sactipeptides,thiopeptides,microviridins,cyanobactins,linear thiazole/oxazole-containing peptides and so on.In this review,we summarize bioinformatic strategies that have been developed to identify and prioritize biosynthetic gene clusters(BGCs)encoding RiPPs,and the genome mining-guided discovery of novel RiPPs.We also prospectively provide a vision of what genomics-guided discovery of RiPPs may look like in the future,especially the discovery of RiPPs from dominant but uncultivated microbes,which will be promoted by the combinational use of synthetic biology and metagenome mining strategies.
