Two genetic linkage maps, constructed by DH and RILs populations derived from the same parents, were carried out for the identification and comparison of QTLs controlling yield traits across different years in rice （...Two genetic linkage maps, constructed by DH and RILs populations derived from the same parents, were carried out for the identification and comparison of QTLs controlling yield traits across different years in rice （Oryza sativa L.）. A total of 194 SSR and STS markers were used in two maps, of which 114 markers were same. The distribution of Samgang allele was higher in RILs population than it in DH population. Comparing with DH population, RILs population has more lines with higher yield and wider phenotypic transgressive segression for yield traits. Although most of QTLs for the same trait were different in two populations across different years, 8 QTLs （including gwp 11.1, spp5.1, spp 10.1, spp 11.2, ssrl. 1, ssrl 1.1, tgw9.1 and tgwl 1.1） were detected over 2 yr. It is important to note that pppl0.1, sppl0.1 and tgw9.1 were identified in two populations, while sppl 0.1 and tgw9.1 were simultaneity observed across different years. Epistatic effects were more important than additive effects for PPP, SPP, yield in DH population and TGW, yield in R/Ls population. Epistatic effects of DH and RILs populations were different on the same genetic background in the present study, which illuminated the QE interaction played an important role on epistatic effect. Identification and comparison of QTLs for yield traits in DH and RILs populations should provide various and more precise information. The QTLs identified in present study would be valuable in marker-assisted selection program for improving rice yield.展开更多
Recombinant inbred lines(RILs) serve as powerful tools for genetic mapping.RILs are obtained by crossing two inbred lines followed by repeated selfing or sib-mating to create a set of new
以来自于玉米杂交种农大108(许178×黄C)的一套包含166个家系的重组自交系群体为基础,组配包含243个杂交组合的IF_2群体。2013年和2014年分别在郑州和安阳进行田间试验,通过两年两点的田间试验,利用复合区间作图法对玉米穗上节间数(...以来自于玉米杂交种农大108(许178×黄C)的一套包含166个家系的重组自交系群体为基础,组配包含243个杂交组合的IF_2群体。2013年和2014年分别在郑州和安阳进行田间试验,通过两年两点的田间试验,利用复合区间作图法对玉米穗上节间数(Internodes above the upmost ear,IAEs)进行QTL分析,结果表明,IF_2群体的穗上节间数显著高于RIL群体,这表明该性状存在杂种优势。对2个群体的穗上节间数进行单环境和环境间联合QTL分析,共定位到14个QTL,其中,RIL群体共定位到6个QTL,IF_2定位到8个QTL,所有定位到的QTL分布在第1,3,5,6和8染色体上。RIL群体穗上节间数QTL中,qIAEs1a和qIAEs1c分别在多个环境和环境联合中被检测到,贡献率均大于12%。IF_2群体中,qIAEs6b和qIAEs3在多个环境间和环境联合QTL分析中被检测到,但qIAEs3的贡献率较小。在2个群体间并没有定位到相同的QTL,这表明玉米自交系和杂交种调控植株性状所涉及的基因是不同的,在IF_2群体中所定位到的QTL表现出的效应包含有杂种优势。所定位到的主效QTL可能为相应群体中调控玉米穗上节间数的主效QTL。展开更多
基金supported by the Biogreen 21 R&D Program,Rural Development Administration,Republic of Korea(20100301-061-239-001-09-00)the National Agriculture Science Technology Achievement Transformation Fund of China(2011GB2B000006)
文摘Two genetic linkage maps, constructed by DH and RILs populations derived from the same parents, were carried out for the identification and comparison of QTLs controlling yield traits across different years in rice （Oryza sativa L.）. A total of 194 SSR and STS markers were used in two maps, of which 114 markers were same. The distribution of Samgang allele was higher in RILs population than it in DH population. Comparing with DH population, RILs population has more lines with higher yield and wider phenotypic transgressive segression for yield traits. Although most of QTLs for the same trait were different in two populations across different years, 8 QTLs （including gwp 11.1, spp5.1, spp 10.1, spp 11.2, ssrl. 1, ssrl 1.1, tgw9.1 and tgwl 1.1） were detected over 2 yr. It is important to note that pppl0.1, sppl0.1 and tgw9.1 were identified in two populations, while sppl 0.1 and tgw9.1 were simultaneity observed across different years. Epistatic effects were more important than additive effects for PPP, SPP, yield in DH population and TGW, yield in R/Ls population. Epistatic effects of DH and RILs populations were different on the same genetic background in the present study, which illuminated the QE interaction played an important role on epistatic effect. Identification and comparison of QTLs for yield traits in DH and RILs populations should provide various and more precise information. The QTLs identified in present study would be valuable in marker-assisted selection program for improving rice yield.
文摘Recombinant inbred lines(RILs) serve as powerful tools for genetic mapping.RILs are obtained by crossing two inbred lines followed by repeated selfing or sib-mating to create a set of new
文摘以来自于玉米杂交种农大108(许178×黄C)的一套包含166个家系的重组自交系群体为基础,组配包含243个杂交组合的IF_2群体。2013年和2014年分别在郑州和安阳进行田间试验,通过两年两点的田间试验,利用复合区间作图法对玉米穗上节间数(Internodes above the upmost ear,IAEs)进行QTL分析,结果表明,IF_2群体的穗上节间数显著高于RIL群体,这表明该性状存在杂种优势。对2个群体的穗上节间数进行单环境和环境间联合QTL分析,共定位到14个QTL,其中,RIL群体共定位到6个QTL,IF_2定位到8个QTL,所有定位到的QTL分布在第1,3,5,6和8染色体上。RIL群体穗上节间数QTL中,qIAEs1a和qIAEs1c分别在多个环境和环境联合中被检测到,贡献率均大于12%。IF_2群体中,qIAEs6b和qIAEs3在多个环境间和环境联合QTL分析中被检测到,但qIAEs3的贡献率较小。在2个群体间并没有定位到相同的QTL,这表明玉米自交系和杂交种调控植株性状所涉及的基因是不同的,在IF_2群体中所定位到的QTL表现出的效应包含有杂种优势。所定位到的主效QTL可能为相应群体中调控玉米穗上节间数的主效QTL。
