运用高效液相色谱法(HPLC)联用示差折光检测器(RID)建立了黄体酮注射液中羟丙基-β-环糊精的含量测定方法。色谱柱为分子排阻色谱柱(Advance Bio SEC 130?,7.8 mm×300 mm,2.7μm),流动相为乙腈-水(20∶80),检测器为示差折光检测器(...运用高效液相色谱法(HPLC)联用示差折光检测器(RID)建立了黄体酮注射液中羟丙基-β-环糊精的含量测定方法。色谱柱为分子排阻色谱柱(Advance Bio SEC 130?,7.8 mm×300 mm,2.7μm),流动相为乙腈-水(20∶80),检测器为示差折光检测器(RID),温度为40℃,柱温为35℃,进样量为20μL,流动相的流速为0.8 m L·min^(-1)。结果表明羟丙基-β-环糊精在0.286~0.858 mg·m L^(-1)范围内线性关系良好(r=0.9999),羟丙基-β-环糊精的平均回收率为100.4%,RSD为0.4%(n=9)。通过重复性试验、加标回收试验、精密度试验、稳定性试验、耐用性试验和样品含量测定表明,所建立的方法操作简洁、准确可靠,可用于黄体酮注射液的质量控制。展开更多
Objective:To explore whether or not the changes in body surface resistance at yuan-source points of three yin meridians of foot can specifically responds the onset of primary dysmenorrhea(PD) in females of menstrual p...Objective:To explore whether or not the changes in body surface resistance at yuan-source points of three yin meridians of foot can specifically responds the onset of primary dysmenorrhea(PD) in females of menstrual period and the before and after,so as to provide the evidence for acupoint selection in clinic.Methods:A total of 90 cases of PD were recruited in a PD group and 90 healthy female undergraduates were recruited in a normal group.The yuan-source of points of three yin meridians of foot were selected,i.e.Taibai(太白SP3),Taichong(太冲LR3) and Taixi(太溪KI3).The acupoints for control were Xuehai(血海SP10),the non-special acupoint,Xuanzhong(悬钟GB39),the acupoint not on the relevant meridian,as well as a non-meridian point.Successively,before menstruation,on Day 1 during menstruation and on Day 3 after menstruation,the body surface resistance at the above-mentioned test sites was detected in the participants of two groups each day.The resistance imbalance degree(RID) of the bilateral acupoints was taken as the outcome indicator.The observation lasted one menstrual cycle.Results:(1) Intra-group comparison:at LR3,the RID was lower on Day 1 during menstruation than that before menstruation significantly in the normal group(P ≤0.05).There was no significant change in RID at the same time points,but RID on Day 3 after menstruation was lower significantly than that before menstruation in the PD group(P ≤0.05).At SP3,KI3,SP10,GB39 and sham acupoint,there were no significant differences in RID among different time points within the same group(all P> 0.05).(2) Intergroup comparison:there were no significant differences in RID among different time points at the same point.(all P> 0.05).Conclusion:RID at yuan-source points of three yin meridians of foot cannot specifically respond to PD.However,at LR3,before and after menstruation,RID changes are different between PD patients and healthy female undergraduates,which needs a further study in the future.展开更多
目的:建立HPLC-RID法测定灯盏花素葡萄糖注射液中葡萄糖的含量。方法:采用高效液相色谱法,用酰胺基键合硅胶色谱柱(Innovation HP amide规格250mm×4.6mm,5μm,CHROM-MATRIX BIO),以乙腈-水(65∶35)为流动相,用示差折光率检测器检测...目的:建立HPLC-RID法测定灯盏花素葡萄糖注射液中葡萄糖的含量。方法:采用高效液相色谱法,用酰胺基键合硅胶色谱柱(Innovation HP amide规格250mm×4.6mm,5μm,CHROM-MATRIX BIO),以乙腈-水(65∶35)为流动相,用示差折光率检测器检测,流速0.8mL·min^-1,以D-无水葡萄糖为对照品,按外标法计算,灯盏花素葡萄糖注射液中葡萄糖的含量应为标示量的95.0%~105.0%。结果:葡萄糖检测浓度在2.0286~7.10mg·mL^-1的范围内呈良好线性关系,相关系数r=0.9999;葡萄糖的检出限为0.02857mg·mL^-1,定量限为0.09523mg·mL^-1。供试品溶液在12h内稳定,且辅料不干扰。结论:建立的方法精密度和准确度均达到含量测定的要求,适用于灯盏花素葡萄糖注射液中葡萄糖含量的测定。展开更多
文摘运用高效液相色谱法(HPLC)联用示差折光检测器(RID)建立了黄体酮注射液中羟丙基-β-环糊精的含量测定方法。色谱柱为分子排阻色谱柱(Advance Bio SEC 130?,7.8 mm×300 mm,2.7μm),流动相为乙腈-水(20∶80),检测器为示差折光检测器(RID),温度为40℃,柱温为35℃,进样量为20μL,流动相的流速为0.8 m L·min^(-1)。结果表明羟丙基-β-环糊精在0.286~0.858 mg·m L^(-1)范围内线性关系良好(r=0.9999),羟丙基-β-环糊精的平均回收率为100.4%,RSD为0.4%(n=9)。通过重复性试验、加标回收试验、精密度试验、稳定性试验、耐用性试验和样品含量测定表明,所建立的方法操作简洁、准确可靠,可用于黄体酮注射液的质量控制。
基金Supported by National Natural Science Foundation of China:81573884,81973755,81603542Excellent Innovation Team Construction Project of Hebei University of Chinese Medicine:JTZ2020008。
文摘Objective:To explore whether or not the changes in body surface resistance at yuan-source points of three yin meridians of foot can specifically responds the onset of primary dysmenorrhea(PD) in females of menstrual period and the before and after,so as to provide the evidence for acupoint selection in clinic.Methods:A total of 90 cases of PD were recruited in a PD group and 90 healthy female undergraduates were recruited in a normal group.The yuan-source of points of three yin meridians of foot were selected,i.e.Taibai(太白SP3),Taichong(太冲LR3) and Taixi(太溪KI3).The acupoints for control were Xuehai(血海SP10),the non-special acupoint,Xuanzhong(悬钟GB39),the acupoint not on the relevant meridian,as well as a non-meridian point.Successively,before menstruation,on Day 1 during menstruation and on Day 3 after menstruation,the body surface resistance at the above-mentioned test sites was detected in the participants of two groups each day.The resistance imbalance degree(RID) of the bilateral acupoints was taken as the outcome indicator.The observation lasted one menstrual cycle.Results:(1) Intra-group comparison:at LR3,the RID was lower on Day 1 during menstruation than that before menstruation significantly in the normal group(P ≤0.05).There was no significant change in RID at the same time points,but RID on Day 3 after menstruation was lower significantly than that before menstruation in the PD group(P ≤0.05).At SP3,KI3,SP10,GB39 and sham acupoint,there were no significant differences in RID among different time points within the same group(all P> 0.05).(2) Intergroup comparison:there were no significant differences in RID among different time points at the same point.(all P> 0.05).Conclusion:RID at yuan-source points of three yin meridians of foot cannot specifically respond to PD.However,at LR3,before and after menstruation,RID changes are different between PD patients and healthy female undergraduates,which needs a further study in the future.
文摘目的:建立HPLC-RID法测定灯盏花素葡萄糖注射液中葡萄糖的含量。方法:采用高效液相色谱法,用酰胺基键合硅胶色谱柱(Innovation HP amide规格250mm×4.6mm,5μm,CHROM-MATRIX BIO),以乙腈-水(65∶35)为流动相,用示差折光率检测器检测,流速0.8mL·min^-1,以D-无水葡萄糖为对照品,按外标法计算,灯盏花素葡萄糖注射液中葡萄糖的含量应为标示量的95.0%~105.0%。结果:葡萄糖检测浓度在2.0286~7.10mg·mL^-1的范围内呈良好线性关系,相关系数r=0.9999;葡萄糖的检出限为0.02857mg·mL^-1,定量限为0.09523mg·mL^-1。供试品溶液在12h内稳定,且辅料不干扰。结论:建立的方法精密度和准确度均达到含量测定的要求,适用于灯盏花素葡萄糖注射液中葡萄糖含量的测定。
