建立了高效液相色谱法（HPLE）测定三七消栓片中三七皂苷R1的含量。采用Phenomenex Luna C18（4．6×250mm，5μm）色谱柱，以乙腈和水为流动相进行梯度洗脱，流速为1．0mL／min，柱温30℃，检测波长203nm。在此条件下三七皂苷R1在0...建立了高效液相色谱法（HPLE）测定三七消栓片中三七皂苷R1的含量。采用Phenomenex Luna C18（4．6×250mm，5μm）色谱柱，以乙腈和水为流动相进行梯度洗脱，流速为1．0mL／min，柱温30℃，检测波长203nm。在此条件下三七皂苷R1在0．02922—1．984mg／mL的浓度范围内线性良好（r=0．9999），平均加样回收率为99．37％，RSD为2．1％。该方法简单、准确，专属性强，重现性好，可用于三七消栓片中三七皂苷R1指标的质量控制。展开更多
[Objectives]This study aimed to evaluate the quality of Bupleurum falcatum Linne interplanted with walnut in Ganluo and study the development and utilization value of other parts.[Methods]The contents of saikosaponins...[Objectives]This study aimed to evaluate the quality of Bupleurum falcatum Linne interplanted with walnut in Ganluo and study the development and utilization value of other parts.[Methods]The contents of saikosaponins a and d were determined with RP-HPLC,and the contents of 7 kinds of harmful elements were determined by ICP-MS.The quality of B.falcatum Linne was compared with that of other Bupleurum species.[Results]The total saikosaponin content in Radix Bupleurum Falcatum(15.894 mg/g)was higher than that in Radix Bupleuri of other origins(8.748 mg/g).The total saikosaponin content in leaves of B.falcatum Linne(7.518 mg/g)is more than twice the limit promulgated by Chinese Pharmacopoeia.The contents of Al,Cr,Cu,As,Cd,Pb and Hg in B.falcatum Linne were all lower than the limits promulgated by the pharmacopoeia.In short,the quality of Radix Bupleurum Falcatum was better than that of Radix Bupleurum Marginatum(S3,S4)and Radix Bupleurum Sachalinense(S5).The leaves of B.falcatum Linne contained more saikosaponins and less harmful elements.[Conclusions]The method and technology of interplanting B.falcatum Linne with walnut in Ganluo are mature.The quality of the medicinal materials produced is superior,and the leaves are also rich in saikosaponins a and d,and can be used as the raw material for extracting saikosaponins a and d.This study provides a basis for further in-depth research on the cultivation of B.falcatum Linne in the domestic market.展开更多
目的建立复方甘露醇注射液中甘露醇、葡萄糖含量测定方法。方法用HPLC-RI法,以强酸性阳离子交换树脂(钙型)(Sugar Pak Ⅰ 300mm×6.5mm)为固定相,水为流动相,柱温(85±1)℃,流速0.5mL.min-1;检测器为示差折光检测器,池温40℃。...目的建立复方甘露醇注射液中甘露醇、葡萄糖含量测定方法。方法用HPLC-RI法,以强酸性阳离子交换树脂(钙型)(Sugar Pak Ⅰ 300mm×6.5mm)为固定相,水为流动相,柱温(85±1)℃,流速0.5mL.min-1;检测器为示差折光检测器,池温40℃。结果甘露醇在0.15～15mg·mL-1内呈良好的线性关系,Y=643467X-15373(r=0.9998,n=7),平均回收率为100.1%(RSD=0.89%,n=9);葡萄糖在0.05～5mg·mL-1内呈良好的线性关系,Y=640151X-3719.6(r=1.0000,n=7),平均回收率为100.1%(RSD=0.73%,n=9)。结论该方法简便准确,重复性好,符合甘露醇、葡萄糖含量测定的要求。展开更多
目的建立测定复方思日颗粒中绿原酸含量的方法。方法采用Waters Sun Fire TMCis（150mm×4．6mm，5μm）色谱柱，流动相为甲醇（A）-乙腈（B）-0．4％磷酸水溶液（C），梯度洗脱（0～50min，5．5％A-6％A，2．9％B，91．6％C→91．1...目的建立测定复方思日颗粒中绿原酸含量的方法。方法采用Waters Sun Fire TMCis（150mm×4．6mm，5μm）色谱柱，流动相为甲醇（A）-乙腈（B）-0．4％磷酸水溶液（C），梯度洗脱（0～50min，5．5％A-6％A，2．9％B，91．6％C→91．1％C），柱温30℃，流速1．0mL／min，检测波长327nm，进样量15μL.结果绿原酸对照品在4．24～25．44μg／mL浓度范围内与峰面积的线性关系良好。加标回收率为98．739％（n=9），RSD为2．622％，分离度〉1．5，对称因子不得低于0．7。结论HPLC方法简便、结果准确，可用于复方思日颗粒中绿原酸的含量测定。展开更多
文摘建立了高效液相色谱法（HPLE）测定三七消栓片中三七皂苷R1的含量。采用Phenomenex Luna C18（4．6×250mm，5μm）色谱柱，以乙腈和水为流动相进行梯度洗脱，流速为1．0mL／min，柱温30℃，检测波长203nm。在此条件下三七皂苷R1在0．02922—1．984mg／mL的浓度范围内线性良好（r=0．9999），平均加样回收率为99．37％，RSD为2．1％。该方法简单、准确，专属性强，重现性好，可用于三七消栓片中三七皂苷R1指标的质量控制。
基金Science and Technology Plan Project of Sichuan Province(2018NZ0113)Technology Research and Development and Promotion and Application Project of Liangshan Prefecture(17YYJS0084)Fundamental Research Funds for the Central Universities(2018NQN13).
文摘[Objectives]This study aimed to evaluate the quality of Bupleurum falcatum Linne interplanted with walnut in Ganluo and study the development and utilization value of other parts.[Methods]The contents of saikosaponins a and d were determined with RP-HPLC,and the contents of 7 kinds of harmful elements were determined by ICP-MS.The quality of B.falcatum Linne was compared with that of other Bupleurum species.[Results]The total saikosaponin content in Radix Bupleurum Falcatum(15.894 mg/g)was higher than that in Radix Bupleuri of other origins(8.748 mg/g).The total saikosaponin content in leaves of B.falcatum Linne(7.518 mg/g)is more than twice the limit promulgated by Chinese Pharmacopoeia.The contents of Al,Cr,Cu,As,Cd,Pb and Hg in B.falcatum Linne were all lower than the limits promulgated by the pharmacopoeia.In short,the quality of Radix Bupleurum Falcatum was better than that of Radix Bupleurum Marginatum(S3,S4)and Radix Bupleurum Sachalinense(S5).The leaves of B.falcatum Linne contained more saikosaponins and less harmful elements.[Conclusions]The method and technology of interplanting B.falcatum Linne with walnut in Ganluo are mature.The quality of the medicinal materials produced is superior,and the leaves are also rich in saikosaponins a and d,and can be used as the raw material for extracting saikosaponins a and d.This study provides a basis for further in-depth research on the cultivation of B.falcatum Linne in the domestic market.
文摘目的建立复方甘露醇注射液中甘露醇、葡萄糖含量测定方法。方法用HPLC-RI法,以强酸性阳离子交换树脂(钙型)(Sugar Pak Ⅰ 300mm×6.5mm)为固定相,水为流动相,柱温(85±1)℃,流速0.5mL.min-1;检测器为示差折光检测器,池温40℃。结果甘露醇在0.15～15mg·mL-1内呈良好的线性关系,Y=643467X-15373(r=0.9998,n=7),平均回收率为100.1%(RSD=0.89%,n=9);葡萄糖在0.05～5mg·mL-1内呈良好的线性关系,Y=640151X-3719.6(r=1.0000,n=7),平均回收率为100.1%(RSD=0.73%,n=9)。结论该方法简便准确,重复性好,符合甘露醇、葡萄糖含量测定的要求。
文摘目的建立测定复方思日颗粒中绿原酸含量的方法。方法采用Waters Sun Fire TMCis（150mm×4．6mm，5μm）色谱柱，流动相为甲醇（A）-乙腈（B）-0．4％磷酸水溶液（C），梯度洗脱（0～50min，5．5％A-6％A，2．9％B，91．6％C→91．1％C），柱温30℃，流速1．0mL／min，检测波长327nm，进样量15μL.结果绿原酸对照品在4．24～25．44μg／mL浓度范围内与峰面积的线性关系良好。加标回收率为98．739％（n=9），RSD为2．622％，分离度〉1．5，对称因子不得低于0．7。结论HPLC方法简便、结果准确，可用于复方思日颗粒中绿原酸的含量测定。
