The Mongolian gerbil has been widely used in many research fields and has been reported to be a diurnal laboratory animal. The circadian rhythmicity of these gerbils was investigated in the present study by measuring ...The Mongolian gerbil has been widely used in many research fields and has been reported to be a diurnal laboratory animal. The circadian rhythmicity of these gerbils was investigated in the present study by measuring two hormones that show daily oscillations, cortisol and ACTH, in serum using ELISA kits. The levels of the two hormones were highest at 8:00 am and their rhythmic changes were similar to those in humans. In addition, the influence of stress of handling and blood collection on the physiological parameters of the gerbils was examined. After adaptation to handling for 1 week, some serum parameters in the animals changed. Handling and blood collection did not impact significantly on the following parameters: creatine kinase (CK), lactate dehydrogenase (LD), alanine aminotransferase (ALT), aspartate transaminase (AST), blood urea nitrogen (BUN), and albumin (ALB). However, blood glucose (GLU), total protein (TP) and globulin (GLB) significantly increased while creatinine (CRE) and albumin/globulin (A/G) significantly decreased after adaptation. This work further confirms that the Mongolian gerbil is a diurnal animal and also indicates that a suitable adaptation procedure is necessary for getting reliable results when performing experiments using these animals.展开更多
In the dim light of Studio A at the Jack Doyle Athletics and Recreation Centre,the air is thick with sweat and the sounds of focused concentrated effort.The rhythmic pounding of gloves hitting bags and the steady boun...In the dim light of Studio A at the Jack Doyle Athletics and Recreation Centre,the air is thick with sweat and the sounds of focused concentrated effort.The rhythmic pounding of gloves hitting bags and the steady bounce of jump ropes fill the room.This is my hideaway—a place where every strike and kick is a conversation with myself.展开更多
BACKGROUND Parthenolide(PTL),a sesquiterpene lactone derived from the medicinal herb Chrysanthemum parthenium,exhibits various biological effects by targeting NF-kB,STAT3,and other pathways.It has emerged as a promisi...BACKGROUND Parthenolide(PTL),a sesquiterpene lactone derived from the medicinal herb Chrysanthemum parthenium,exhibits various biological effects by targeting NF-kB,STAT3,and other pathways.It has emerged as a promising adjunct therapy for multiple malignancies.AIM To evaluate the in vitro and in vivo effect of PTL on cyclophosphamide(CTX)metronomic chemotherapy.METHODS The cytotoxicity of PTL and CTX on Lewis lung cancer cells(LLC cells)was assessed by measuring cell activity and apoptosis.The anti-tumor efficiency was evaluated using a tumor xenograft mice model,and the survival of mice and tumor volume were monitored.Additionally,the collected tumor tissues were analyzed for tumor microenvironment indicators and inflammatory factors.RESULTS In vitro,PTL demonstrated a synergistic effect with CTX in inhibiting the growth of LLC cells and promoting apoptosis.In vivo,metronomic chemotherapy com-bined with PTL and CTX improved the survival rate of tumor-bearing mice and reduced tumor growth rate.Furthermore,metronomic chemotherapy combined with PTL and CTX reduced NF-κB activation and improved the tumor immune microenvironment by decreasing tumor angiogenesis,reducing Transforming growth factorβ,andα-SMA positive cells.CONCLUSION PTL is an efficient compound that enhances the metronomic chemotherapy effects of CTX both in vitro and in vivo,suggesting its potential as a supplementary therapeutic strategy in metronomic chemotherapy to improve the chemotherapy effects.展开更多
Lithological, petrographic, and morphoscopic studies were conducted on cuttings and cores from three boreholes drilled in the Loemé salt, Kanga site, Republic of the Congo, to determine 1) the preferential condit...Lithological, petrographic, and morphoscopic studies were conducted on cuttings and cores from three boreholes drilled in the Loemé salt, Kanga site, Republic of the Congo, to determine 1) the preferential conditions for crystallization of carnallite and associated salts and 2) to reconstruct paleoenvironmental and paleoclimatic conditions at the time of sedimentation. Sequential analysis of logs, sedimentary structures, carnallitite facies and associated salts concluded to the existence of a potassic carnallitite lagoon basin with low water cover, on a very wide and extensive plateau, affected by coastal waves and swells resulting from successive collapses. This basin evolved in two phases: confined and then open. The regular stratifications of halite, the rhythmicity of the halite-carnallitite elementary sequences are characteristic of salts that precipitated in relatively stable brines. These salts are therefore tectonosedimentary. The brecciated facies of the carnallitites sometimes associated with tachyhydrite result from the evolution of these deposits into salt crusts reworked by the surges into subaquatic allochemical gravelly cords under water. These crusts mark stages of partial and complete drying of the basin in a very hot and arid climate. Prolonged exposure of halite brines as well as their homogenization by surges accelerated evaporation and their abrupt evolution into carnallite brines obstructing the fossilization of sylvite. The precipitation of tachyhydrite marks the final stage of the successive complete drying of the basin.展开更多
Human brain is hypothesized to store a geometry and dynamic model of the limb.A multilayer perceptron (or MLP) network is used to stand for the model.In this paper the human elbow joint rhythmic movement is simulated ...Human brain is hypothesized to store a geometry and dynamic model of the limb.A multilayer perceptron (or MLP) network is used to stand for the model.In this paper the human elbow joint rhythmic movement is simulated in three cases:1)Parameters of the MLP,the limb geometry and dynamic model match completely,2)Parameters mismatch between them,and 3)Disturbance exists.The results show that parameters mismatch is the main error source,which causes the elbow joint movement to be aberrant.From this we can infer that movement study is a process in which the internal model is updated continuously to match the geometry and dynamic model of limb.展开更多
Objective To investigate the relationship between serum adiponectin levels with adiposity,glucocorticoids, insulin and leptin in Cushing’ s syndrome, obesity and non-obese subjects. Methods The serumadiponectin conce...Objective To investigate the relationship between serum adiponectin levels with adiposity,glucocorticoids, insulin and leptin in Cushing’ s syndrome, obesity and non-obese subjects. Methods The serumadiponectin concentrations were measured in 104 non-obese and 57 overweight or obese subjects byRIA. 15 patients with Cushing’ s syndrome, 10 with obesity and 9 non-obese subjects were investigated, with theirserum adiponectin, glucocorticoids, insulin and leptin levels measured at 8:00, 12:00, 16:00, 20:00, 24:00 and3:00. Dexamethasone suppression tests in both obesity and Cushing’s syndrome were performed at the dose of Img,2mg and 5mg. Results The serum adiponectin concentrations in non-obese were (10. 15 ±6. 33)mg/L in maleand (13. 82 ±6. 09)mg/L in female, and those in overweight or obese ones were (5. 78 ±3. 55)mg/L in male and(8. 13 ± 4. 32) mg/L in female. In both men and women, the fasting adiponectin levels in overweight or obese sub-jects were lower than those of the non-obese ones, and serum adiponectin concentrations were significantly nagetivelycorrelated with BMI, % Fat and waist circumference. The circadian rhythmicity of adiponectin was not distinct, butthe adiponectin levels in obesity were lower than those of the non-obese subjects at all 6 time spots. The serum adi-ponectin area under curve (AUC) were significantly nagetively correlated with BMI, waist circumference and insulinA UC. The adiponectin levels with dexamethasone administration for a short-term both at higher doses and lower do-ses did not change, but was decreased after surgery. Conclusion Adiponectin is a hormone secreted by adipo-cytes which may intimately related to obese and insulin resistance. Therefore, any treatment that could be used to in-crease adiponectin should be beneficial. Neither long-term endogenous hyper-glucocorticoid nor short-term dexam-ethasone administration may affect the adiponectin levels, and similarly, no change with elevated postprandial insu-lin levels.展开更多
Plant leaves respond to day/night cycling in a number of physiological ways. At the mRNA level, the expression of some genes changes during the 24 h period. To determine which proteins exhibited a rhythmic pattern of ...Plant leaves respond to day/night cycling in a number of physiological ways. At the mRNA level, the expression of some genes changes during the 24 h period. To determine which proteins exhibited a rhythmic pattern of expression, proteomic profiles in maize seedling leaves were analyzed by high-throughput two-dimensional gel electrophoresis, combined with MALDI-TOF MS technology. Of the 464 proteins that were detected with silver staining in a pH range of 4-7, 17 （3.66%） showed clock rhythmicity in their abundance. These proteins belonged to diverse functional groups and proteins involved in photosynthesis and carbon metabolism were over-represented. These findings provide a new perspective on the relationship between the physiological functions of leaves and the clock rhythmic system.展开更多
Many behavioral activities of the horseshoe crab Limulus are rhythmic, and most of these are produced in large part by central pattern generators within the CNS. The chain of opisthosomal （‘abdominal＇） ganglia con...Many behavioral activities of the horseshoe crab Limulus are rhythmic, and most of these are produced in large part by central pattern generators within the CNS. The chain of opisthosomal （‘abdominal＇） ganglia controls gill movements of ventilation and gill cleaning, and the prosomal ring of fused ganglia （brain and segmental ‘thoracic＇ ganglia） controls generation of feeding and locomotor movements of the legs. Both the opisthosomal CNS and the prosomal CNS can generate behaviorally ap- propriate patterns of motor output in isolation, without movements or sensory input. Preparations of the isolated opisthosomal CNS generate rhythmic output patterns of motor activity characterized as fictive ventilatory and gill cleaning rhythms. Moreover, CNS preparations also express longer-term patterns, such as intermittent ventilation or sequential bouts of ventilation and gill cleaning. Such longer-term patterns are commonly observed in intact animals. The isolated prosomal CNS does not spontaneously generate the activity patterns characteristic of walking, swimming, and feeding. However, perfusion of octopamine in the isolated prosomal CNS activates central pattern generators underlying rhythmic chewing movements, and injection of octopamine into in- tact Limulus promotes the chewing pattern of feeding, whether or not food is presented. Our understanding of the ability of neu-romodulators such as octopamine to elicit or alter central motor programs may help to clarify the central neural circuits of pattern generation that oroduce and coordinate these rhythmic behaviors展开更多
Purpose:The aim of this study was to examine systemic responses of oxidant/antioxidant status following 2 training sessions of different intensity in amateur rhythmic gymnasts.Methods:Before the experimental training,...Purpose:The aim of this study was to examine systemic responses of oxidant/antioxidant status following 2 training sessions of different intensity in amateur rhythmic gymnasts.Methods:Before the experimental training,10 female gymnasts performed a gradually increased exercise test to assess maximal heart rate,maximal oxygen consumption,and anaerobic threshold.They executed 2 intermittent training sessions separated by 48 h of recovery(48 h-post R):the first was performed at low-moderate intensity(LMI)and the second at high intensity(HI).Blood samples were collected immediately preand post-training and 48 h-post R.Hydroperoxide level(OxL)and total antioxidant capacity(TAC)were photometrically measured.Results:OxL was significantly higher in post-training and 48 h-post R following HI than the same conditions after an LMI session(HI vs.LMI post-training:381.10±46.17(mean±SD)vs.344.18±27.94 Units Carratelli(U.CARR);48 h-post R:412.21±26.61 vs.373.80±36.08 U.CARR).There was no change in TAC between the 2 training sessions investigated.In LMI training,OxL significantly decreased in post-training and increased to reach the baseline at 48 h-post R,whereas TAC increased only at 48 h-post R.In HI training,OxL significantly increased to reach a high oxidative stress 48 h-post R,whereas TAC was lower in post-training than pre-training.Conclusion:The pattern of OxL and TAC levels implies different regulation mechanisms by HI and LMI training sessions.High oxidative stress induced by an HI protocol might be associated with both insufficient TAC and recovery time at 48 h necessary to restore redox balance.展开更多
This study＇s objective was to investigate the Guguan-Xiangong Fault, which lies in the southern Liupanshan area, through satellite image interpretation and field observations. Guguan- Xiangong Fault is divided into f...This study＇s objective was to investigate the Guguan-Xiangong Fault, which lies in the southern Liupanshan area, through satellite image interpretation and field observations. Guguan- Xiangong Fault is divided into five subsegments; among these, the Badu-Longwei segment has been the most recently active. The geomorphic features of the Badu-Longwei segment are clearly displayed, including multiple high fault scarps with fresh bedrock free faces. There is significant evidence for Holocene activity of the three fault sections, located in Renhuashu, Tianjiagou, and Xinjiecun respectively. The three sections feature distinct episodic deposition and fault scratches. Based on 14C- dating and field observations on the three fault sections, two or more paleoearthquakes across the Badu-Longwei fault segment are ascertained, between 5874±116 and 5430±140 a BP, and after 2037±83 a BP respectively. The Badu-Longwei segment of the Guguan-Xiangong Fault is preliminarily extrapolated as the seismogenic structure of the 600 A.D. Qin-Long earthquake.展开更多
Prenatal alcohol exposure disrupts the development of normal fetal respiratory function, but whether it perturbs respiratory rhythmical discharge activity is unclear. Furthermore, it is unknown whether the 5-hydroxytr...Prenatal alcohol exposure disrupts the development of normal fetal respiratory function, but whether it perturbs respiratory rhythmical discharge activity is unclear. Furthermore, it is unknown whether the 5-hydroxytryptamine 2A receptor（5-HT2AR） is involved in the effects of prenatal alcohol exposure. In the present study, pregnant female rats received drinking water containing alcohol at concentrations of 0%, 1%, 2%, 4%, 8% or 10%（v/v） throughout the gestation period. Slices of the medulla from 2-day-old neonatal rats were obtained to record respiratory rhythmical discharge activity. 5-HT2 AR protein and m RNA levels in the pre-B？tzinger complex of the respiratory center were measured by western blot analysis and quantitative RT-PCR, respectively. Compared with the 0% alcohol group, respiratory rhythmical discharge activity in medullary slices in the 4%, 8% and 10% alcohol groups was decreased, and the reduction was greatest in the 8% alcohol group. Respiratory rhythmical discharge activity in the 10% alcohol group was irregular. Thus, 8% was the most effective alcohol concentration at attenuating respiratory rhythmical discharge activity. These findings suggest that prenatal alcohol exposure attenuates respiratory rhythmical discharge activity in neonatal rats by downregulating 5-HT2 AR protein and m RNA levels.展开更多
Based on Matsuoka＇s central pattern generator （CPG） model and taking quadruped as an example, the dynamics of CPG model was investigated through the single-parameter-analysis method and the numerical simulation tec...Based on Matsuoka＇s central pattern generator （CPG） model and taking quadruped as an example, the dynamics of CPG model was investigated through the single-parameter-analysis method and the numerical simulation technique. Simulation results indicate that the CPG model exhibits complex dynamics, while each parameter has specifically definitive influence trends on the CPG output. These conclusions were applied to control a quadrupedal robot to walk in different gaits, clear obstacle, and walk up- and down-slope successfully.展开更多
The electroencephalographic research has focused on the variety of responses to emotional auditory stimulation in patients at different stages after craniocerebral trauma. The patients sampling consisted of three grou...The electroencephalographic research has focused on the variety of responses to emotional auditory stimulation in patients at different stages after craniocerebral trauma. The patients sampling consisted of three groups: 13 comatose patients, 14 severe TBI patients, 12 moderate TBI patients, the control group consisting of the 28 healthy adults. The subjects were stimulated with auditory stimuli containing significant sounds (coughing, laughing, crying, bird singing, barking, scraping) and control sounds (white noise). We have analyzed statistically significant differences of power of the rhythmic activity registered during the presentation of different types of stimuli using Matlab. The t-test differences for each type of stimuli and the background were calculated as well as major ANOVA-effects. The results showed that EEG-response was based on the emotional stimuli valence, the consciousness levels, the severity of injury and the recovery process. The TBI patients showed lower theta-rhythm power in the frontal areas in response to the all emotional stimuli. The alpha-activity was reduced in the TBI patients: the alpha-rhythm depression was most vividly pronounced in the control group. The alpha-rhythm acceleration in the occipital areas was found only in the moderate TBI patients and only to the emotional stimuli. The severe TBI and comatose patients showed lower response rates to the neutral stimuli and higher response rates to the unpleasant physiological stimuli.展开更多
Based on core,thin section,X-ray diffraction,rock pyrolysis,CT scanning,nuclear magnetic resonance and oil testing data,the macro and micro components,sedimentary structure characteristics,of Paleogene Kong 2 Member i...Based on core,thin section,X-ray diffraction,rock pyrolysis,CT scanning,nuclear magnetic resonance and oil testing data,the macro and micro components,sedimentary structure characteristics,of Paleogene Kong 2 Member in Cangdong sag of Huanghua depression and evaluation standard and method of shale oil reservoir were studied to sort out the best shale sections for shale oil horizontal wells.According to the dominant rock type,rhythmic structure and logging curve characteristics,four types of shale lithofacies were identified,namely,thin-layered dolomitic shale,lamellar mixed shale,lamellar felsic shale,and bedded dolomitic shale,and the Kong 21 sub-member was divided into four quasi-sequences,PS1 to PS4.The PS1 shale has a porosity higher than 6%,clay content of less than 20%,and S1 of less than 4 mg/g;the PS2 shale has well-developed laminar structure,larger pore and throat size,better connectivity of pores and throats,high contents of TOC and movable hydrocarbon,S1 of over 4 mg/g,clay content of less than 20%,and porosity of more than 4%;PS3 shale has S1 value higher than 6 mg/g and clay content of 20%-30%,and porosity of less than 4%;and PS4 shale has lower TOC content and low oil content.Shale oil reservoir classification criterion based on five parameters,free hydrocarbon content S1,shale rhythmic structure,clay content,TOC and porosity,was established.The evaluation method of shale oil sweet spot by using the weighted five parameters,and the evaluation index EI were proposed.Through comprehensive analysis,it is concluded that PS2 is best in quality and thus the dual geological and engineering sweet spot of shale oil,PS3 and PS1 come next,the former is more geologic sweet spot,the latter more engineering sweet spot,and PS4 is the poorest.Several vertical and horizontal wells drilled in the PS2 and PS3 sweet spots obtained high oil production.Among them,Well 1701 H has produced stably for 623 days,with cumulative production of over 10000 tons,showing bright exploration prospects of Kong 2 Member shale oil.展开更多
In a number of experiments, when detecting particles emitted in beta decays, periodic oscillations of count rate with an amplitude up to tenths of a percent and short bursts vastly exceeding the usual count rate are f...In a number of experiments, when detecting particles emitted in beta decays, periodic oscillations of count rate with an amplitude up to tenths of a percent and short bursts vastly exceeding the usual count rate are found. At the same time, several experiments did not detect any differences from the “normal” course of beta decays greater than 0.01%. The article shows that the inconsistency of the experimental results is due to different measurement technique. The assumption is made of the possible participation in the beta decay processes of cosmic slow neutrinos, which makes it possible to explain in a comprehensive manner not only periodic and sporadic changes in the beta decay rate, but also a number of other incomprehensible phenomena associated with beta radioactivity. On the basis of the experiments carried out, an estimate is made of the flux density of slow cosmic neutrinos.展开更多
Permian salt-bearing formation covers a substantial area of the Russian Plate.In some places,it is up to several kilometers thick.The salt-bearing formation contains unique accumulations of potassium and magnesium salts,
The rhythmic movement is a spontaneous behavior due to the central pattern generator （CPG）. At present, the CPG model only shows the spontaneous behavior, but does not refer to the instruction regulation role of the...The rhythmic movement is a spontaneous behavior due to the central pattern generator （CPG）. At present, the CPG model only shows the spontaneous behavior, but does not refer to the instruction regulation role of the cerebral cortex. In this paper, a modified model based on the Matsuoka neural oscillator theory is presented to better show the regulation role of the cerebral cortex signal to the CPG neuronal network. The complex interaction between the input signal and other parameters in the CPG network is established, making all parameters of the CPG vary with the input signal. In this way, the effect of the input signal to the CPG network is enhanced so that the CPG network can express the self-regulation movement state instead of being limited to the spontaneous behavior, and thus the regulation role of the cerebral cortex signal can be reflected. Numerical simulation shows that the modified model can generate various movement forms with different modes, frequencies, and interchanges between them. It is revealed in theories that the cerebral cortex signal can regulate the mode and frequency of the gait in the ~ourse of the gait movement.展开更多
Both external and endogenous electrical fields widely exist in the environment of cortical neurons. The effects of a weak alternating current （AC） field on a neural network model with synaptic plasticity are studied...Both external and endogenous electrical fields widely exist in the environment of cortical neurons. The effects of a weak alternating current （AC） field on a neural network model with synaptic plasticity are studied. It is found that self-sustained rhythmic firing patterns, which are closely correlated with the cognitive functions, are significantly modified due to the self-organizing of the network in the weak AC field. The activities of the neural networks are affected by the synaptic connection strength, the exterrtal stimuli, and so on. In the presence of learning rules, the synaptic connections can be modulated by the external stimuli, which will further enhance the sensitivity of the network to the external signal. The properties of the external AC stimuli can serve as control parameters in modulating the evolution of the neural network.展开更多
We examined theta activity in the temporal hippocampus of ure-thane-chloralose-anesthetized pigs by stimulation with two chemical odors, beta-phenyl ethyl alcohol (PEA;rose-like odor) and n-amyl acetate (banana-like o...We examined theta activity in the temporal hippocampus of ure-thane-chloralose-anesthetized pigs by stimulation with two chemical odors, beta-phenyl ethyl alcohol (PEA;rose-like odor) and n-amyl acetate (banana-like odor). Neural activity was recorded in the neural cell layer nearby fimbria of the temporal hippocampus in three of six animals. Odor stimulation with PEA at a low concentration (10-4 w/w;100 ppm) significantly increased power of the low-frequency theta band (type-2;4 - 6 Hz) for the middle 10 s (w (3, 6, 2.5%) >2.56). The PEA odor at a higher concentration (10-3 w/w;1000 ppm), however, showed a tendency for gradual increase in?the low-frequency theta but the response was insignificant compared with the control. On the other hand, odor stimulation with n-amyl acetate (10-4 w/w;100 ppm) caused no apparent increase or a tendency for decrease in power of the low-frequency theta. Thus, the type-2 theta response in the temporal hippocampus to the PEA odor contrasted strikingly with that to the n-amylacetate in the ure-thane-chloralose-anesthetized pigs. The response of the type-2 theta rhythm in the temporal hippocampus may underlie the difference in emotional sensation and cognition of PEA from n-amyl acetate in the pig.展开更多
基金National Natural Science Foundation of China,Grant/Award Number:No.31572341
文摘The Mongolian gerbil has been widely used in many research fields and has been reported to be a diurnal laboratory animal. The circadian rhythmicity of these gerbils was investigated in the present study by measuring two hormones that show daily oscillations, cortisol and ACTH, in serum using ELISA kits. The levels of the two hormones were highest at 8:00 am and their rhythmic changes were similar to those in humans. In addition, the influence of stress of handling and blood collection on the physiological parameters of the gerbils was examined. After adaptation to handling for 1 week, some serum parameters in the animals changed. Handling and blood collection did not impact significantly on the following parameters: creatine kinase (CK), lactate dehydrogenase (LD), alanine aminotransferase (ALT), aspartate transaminase (AST), blood urea nitrogen (BUN), and albumin (ALB). However, blood glucose (GLU), total protein (TP) and globulin (GLB) significantly increased while creatinine (CRE) and albumin/globulin (A/G) significantly decreased after adaptation. This work further confirms that the Mongolian gerbil is a diurnal animal and also indicates that a suitable adaptation procedure is necessary for getting reliable results when performing experiments using these animals.
文摘In the dim light of Studio A at the Jack Doyle Athletics and Recreation Centre,the air is thick with sweat and the sounds of focused concentrated effort.The rhythmic pounding of gloves hitting bags and the steady bounce of jump ropes fill the room.This is my hideaway—a place where every strike and kick is a conversation with myself.
基金Supported by Joint Funding of Yunnan Ministry of Science and Technology,No.2019FF002-048and Beijing Heathco Clinical Oncology Research Foundation,No.Y-Q201802-048.
文摘BACKGROUND Parthenolide(PTL),a sesquiterpene lactone derived from the medicinal herb Chrysanthemum parthenium,exhibits various biological effects by targeting NF-kB,STAT3,and other pathways.It has emerged as a promising adjunct therapy for multiple malignancies.AIM To evaluate the in vitro and in vivo effect of PTL on cyclophosphamide(CTX)metronomic chemotherapy.METHODS The cytotoxicity of PTL and CTX on Lewis lung cancer cells(LLC cells)was assessed by measuring cell activity and apoptosis.The anti-tumor efficiency was evaluated using a tumor xenograft mice model,and the survival of mice and tumor volume were monitored.Additionally,the collected tumor tissues were analyzed for tumor microenvironment indicators and inflammatory factors.RESULTS In vitro,PTL demonstrated a synergistic effect with CTX in inhibiting the growth of LLC cells and promoting apoptosis.In vivo,metronomic chemotherapy com-bined with PTL and CTX improved the survival rate of tumor-bearing mice and reduced tumor growth rate.Furthermore,metronomic chemotherapy combined with PTL and CTX reduced NF-κB activation and improved the tumor immune microenvironment by decreasing tumor angiogenesis,reducing Transforming growth factorβ,andα-SMA positive cells.CONCLUSION PTL is an efficient compound that enhances the metronomic chemotherapy effects of CTX both in vitro and in vivo,suggesting its potential as a supplementary therapeutic strategy in metronomic chemotherapy to improve the chemotherapy effects.
文摘Lithological, petrographic, and morphoscopic studies were conducted on cuttings and cores from three boreholes drilled in the Loemé salt, Kanga site, Republic of the Congo, to determine 1) the preferential conditions for crystallization of carnallite and associated salts and 2) to reconstruct paleoenvironmental and paleoclimatic conditions at the time of sedimentation. Sequential analysis of logs, sedimentary structures, carnallitite facies and associated salts concluded to the existence of a potassic carnallitite lagoon basin with low water cover, on a very wide and extensive plateau, affected by coastal waves and swells resulting from successive collapses. This basin evolved in two phases: confined and then open. The regular stratifications of halite, the rhythmicity of the halite-carnallitite elementary sequences are characteristic of salts that precipitated in relatively stable brines. These salts are therefore tectonosedimentary. The brecciated facies of the carnallitites sometimes associated with tachyhydrite result from the evolution of these deposits into salt crusts reworked by the surges into subaquatic allochemical gravelly cords under water. These crusts mark stages of partial and complete drying of the basin in a very hot and arid climate. Prolonged exposure of halite brines as well as their homogenization by surges accelerated evaporation and their abrupt evolution into carnallite brines obstructing the fossilization of sylvite. The precipitation of tachyhydrite marks the final stage of the successive complete drying of the basin.
文摘Human brain is hypothesized to store a geometry and dynamic model of the limb.A multilayer perceptron (or MLP) network is used to stand for the model.In this paper the human elbow joint rhythmic movement is simulated in three cases:1)Parameters of the MLP,the limb geometry and dynamic model match completely,2)Parameters mismatch between them,and 3)Disturbance exists.The results show that parameters mismatch is the main error source,which causes the elbow joint movement to be aberrant.From this we can infer that movement study is a process in which the internal model is updated continuously to match the geometry and dynamic model of limb.
文摘Objective To investigate the relationship between serum adiponectin levels with adiposity,glucocorticoids, insulin and leptin in Cushing’ s syndrome, obesity and non-obese subjects. Methods The serumadiponectin concentrations were measured in 104 non-obese and 57 overweight or obese subjects byRIA. 15 patients with Cushing’ s syndrome, 10 with obesity and 9 non-obese subjects were investigated, with theirserum adiponectin, glucocorticoids, insulin and leptin levels measured at 8:00, 12:00, 16:00, 20:00, 24:00 and3:00. Dexamethasone suppression tests in both obesity and Cushing’s syndrome were performed at the dose of Img,2mg and 5mg. Results The serum adiponectin concentrations in non-obese were (10. 15 ±6. 33)mg/L in maleand (13. 82 ±6. 09)mg/L in female, and those in overweight or obese ones were (5. 78 ±3. 55)mg/L in male and(8. 13 ± 4. 32) mg/L in female. In both men and women, the fasting adiponectin levels in overweight or obese sub-jects were lower than those of the non-obese ones, and serum adiponectin concentrations were significantly nagetivelycorrelated with BMI, % Fat and waist circumference. The circadian rhythmicity of adiponectin was not distinct, butthe adiponectin levels in obesity were lower than those of the non-obese subjects at all 6 time spots. The serum adi-ponectin area under curve (AUC) were significantly nagetively correlated with BMI, waist circumference and insulinA UC. The adiponectin levels with dexamethasone administration for a short-term both at higher doses and lower do-ses did not change, but was decreased after surgery. Conclusion Adiponectin is a hormone secreted by adipo-cytes which may intimately related to obese and insulin resistance. Therefore, any treatment that could be used to in-crease adiponectin should be beneficial. Neither long-term endogenous hyper-glucocorticoid nor short-term dexam-ethasone administration may affect the adiponectin levels, and similarly, no change with elevated postprandial insu-lin levels.
基金supported by the National Basic Research Program of China(2007CB109000)the National Science Found for Distinguished Young Scholars, China(30925023)+1 种基金the National Natural Science Foundation of China(30671297)the National High-Tech R&D Program of China(2009AA101102)
文摘Plant leaves respond to day/night cycling in a number of physiological ways. At the mRNA level, the expression of some genes changes during the 24 h period. To determine which proteins exhibited a rhythmic pattern of expression, proteomic profiles in maize seedling leaves were analyzed by high-throughput two-dimensional gel electrophoresis, combined with MALDI-TOF MS technology. Of the 464 proteins that were detected with silver staining in a pH range of 4-7, 17 （3.66%） showed clock rhythmicity in their abundance. These proteins belonged to diverse functional groups and proteins involved in photosynthesis and carbon metabolism were over-represented. These findings provide a new perspective on the relationship between the physiological functions of leaves and the clock rhythmic system.
基金the U.S. Public Health Service for research grant funding for much of the work in my laboratory
文摘Many behavioral activities of the horseshoe crab Limulus are rhythmic, and most of these are produced in large part by central pattern generators within the CNS. The chain of opisthosomal （‘abdominal＇） ganglia controls gill movements of ventilation and gill cleaning, and the prosomal ring of fused ganglia （brain and segmental ‘thoracic＇ ganglia） controls generation of feeding and locomotor movements of the legs. Both the opisthosomal CNS and the prosomal CNS can generate behaviorally ap- propriate patterns of motor output in isolation, without movements or sensory input. Preparations of the isolated opisthosomal CNS generate rhythmic output patterns of motor activity characterized as fictive ventilatory and gill cleaning rhythms. Moreover, CNS preparations also express longer-term patterns, such as intermittent ventilation or sequential bouts of ventilation and gill cleaning. Such longer-term patterns are commonly observed in intact animals. The isolated prosomal CNS does not spontaneously generate the activity patterns characteristic of walking, swimming, and feeding. However, perfusion of octopamine in the isolated prosomal CNS activates central pattern generators underlying rhythmic chewing movements, and injection of octopamine into in- tact Limulus promotes the chewing pattern of feeding, whether or not food is presented. Our understanding of the ability of neu-romodulators such as octopamine to elicit or alter central motor programs may help to clarify the central neural circuits of pattern generation that oroduce and coordinate these rhythmic behaviors
基金supported by the University of Palermo (FFR 2012-13)
文摘Purpose:The aim of this study was to examine systemic responses of oxidant/antioxidant status following 2 training sessions of different intensity in amateur rhythmic gymnasts.Methods:Before the experimental training,10 female gymnasts performed a gradually increased exercise test to assess maximal heart rate,maximal oxygen consumption,and anaerobic threshold.They executed 2 intermittent training sessions separated by 48 h of recovery(48 h-post R):the first was performed at low-moderate intensity(LMI)and the second at high intensity(HI).Blood samples were collected immediately preand post-training and 48 h-post R.Hydroperoxide level(OxL)and total antioxidant capacity(TAC)were photometrically measured.Results:OxL was significantly higher in post-training and 48 h-post R following HI than the same conditions after an LMI session(HI vs.LMI post-training:381.10±46.17(mean±SD)vs.344.18±27.94 Units Carratelli(U.CARR);48 h-post R:412.21±26.61 vs.373.80±36.08 U.CARR).There was no change in TAC between the 2 training sessions investigated.In LMI training,OxL significantly decreased in post-training and increased to reach the baseline at 48 h-post R,whereas TAC increased only at 48 h-post R.In HI training,OxL significantly increased to reach a high oxidative stress 48 h-post R,whereas TAC was lower in post-training than pre-training.Conclusion:The pattern of OxL and TAC levels implies different regulation mechanisms by HI and LMI training sessions.High oxidative stress induced by an HI protocol might be associated with both insufficient TAC and recovery time at 48 h necessary to restore redox balance.
基金supported by the Chinese Geological Survey (CGS) (Grant No.1212011120181 & Grant No.1212011120115)a key project of the Natural Science Foundation of China (Grant No.41030317)
文摘This study＇s objective was to investigate the Guguan-Xiangong Fault, which lies in the southern Liupanshan area, through satellite image interpretation and field observations. Guguan- Xiangong Fault is divided into five subsegments; among these, the Badu-Longwei segment has been the most recently active. The geomorphic features of the Badu-Longwei segment are clearly displayed, including multiple high fault scarps with fresh bedrock free faces. There is significant evidence for Holocene activity of the three fault sections, located in Renhuashu, Tianjiagou, and Xinjiecun respectively. The three sections feature distinct episodic deposition and fault scratches. Based on 14C- dating and field observations on the three fault sections, two or more paleoearthquakes across the Badu-Longwei fault segment are ascertained, between 5874±116 and 5430±140 a BP, and after 2037±83 a BP respectively. The Badu-Longwei segment of the Guguan-Xiangong Fault is preliminarily extrapolated as the seismogenic structure of the 600 A.D. Qin-Long earthquake.
基金the Natural Science Foundation of Henan Province in China,No.102102310156the Foundation of Xinxiang Technology Bureau in China,No.ZG14004
文摘Prenatal alcohol exposure disrupts the development of normal fetal respiratory function, but whether it perturbs respiratory rhythmical discharge activity is unclear. Furthermore, it is unknown whether the 5-hydroxytryptamine 2A receptor（5-HT2AR） is involved in the effects of prenatal alcohol exposure. In the present study, pregnant female rats received drinking water containing alcohol at concentrations of 0%, 1%, 2%, 4%, 8% or 10%（v/v） throughout the gestation period. Slices of the medulla from 2-day-old neonatal rats were obtained to record respiratory rhythmical discharge activity. 5-HT2 AR protein and m RNA levels in the pre-B？tzinger complex of the respiratory center were measured by western blot analysis and quantitative RT-PCR, respectively. Compared with the 0% alcohol group, respiratory rhythmical discharge activity in medullary slices in the 4%, 8% and 10% alcohol groups was decreased, and the reduction was greatest in the 8% alcohol group. Respiratory rhythmical discharge activity in the 10% alcohol group was irregular. Thus, 8% was the most effective alcohol concentration at attenuating respiratory rhythmical discharge activity. These findings suggest that prenatal alcohol exposure attenuates respiratory rhythmical discharge activity in neonatal rats by downregulating 5-HT2 AR protein and m RNA levels.
文摘Based on Matsuoka＇s central pattern generator （CPG） model and taking quadruped as an example, the dynamics of CPG model was investigated through the single-parameter-analysis method and the numerical simulation technique. Simulation results indicate that the CPG model exhibits complex dynamics, while each parameter has specifically definitive influence trends on the CPG output. These conclusions were applied to control a quadrupedal robot to walk in different gaits, clear obstacle, and walk up- and down-slope successfully.
文摘The electroencephalographic research has focused on the variety of responses to emotional auditory stimulation in patients at different stages after craniocerebral trauma. The patients sampling consisted of three groups: 13 comatose patients, 14 severe TBI patients, 12 moderate TBI patients, the control group consisting of the 28 healthy adults. The subjects were stimulated with auditory stimuli containing significant sounds (coughing, laughing, crying, bird singing, barking, scraping) and control sounds (white noise). We have analyzed statistically significant differences of power of the rhythmic activity registered during the presentation of different types of stimuli using Matlab. The t-test differences for each type of stimuli and the background were calculated as well as major ANOVA-effects. The results showed that EEG-response was based on the emotional stimuli valence, the consciousness levels, the severity of injury and the recovery process. The TBI patients showed lower theta-rhythm power in the frontal areas in response to the all emotional stimuli. The alpha-activity was reduced in the TBI patients: the alpha-rhythm depression was most vividly pronounced in the control group. The alpha-rhythm acceleration in the occipital areas was found only in the moderate TBI patients and only to the emotional stimuli. The severe TBI and comatose patients showed lower response rates to the neutral stimuli and higher response rates to the unpleasant physiological stimuli.
基金Supported by the China Petroleum Science and Technology Major Project(2018E-1,2019E-2601)。
文摘Based on core,thin section,X-ray diffraction,rock pyrolysis,CT scanning,nuclear magnetic resonance and oil testing data,the macro and micro components,sedimentary structure characteristics,of Paleogene Kong 2 Member in Cangdong sag of Huanghua depression and evaluation standard and method of shale oil reservoir were studied to sort out the best shale sections for shale oil horizontal wells.According to the dominant rock type,rhythmic structure and logging curve characteristics,four types of shale lithofacies were identified,namely,thin-layered dolomitic shale,lamellar mixed shale,lamellar felsic shale,and bedded dolomitic shale,and the Kong 21 sub-member was divided into four quasi-sequences,PS1 to PS4.The PS1 shale has a porosity higher than 6%,clay content of less than 20%,and S1 of less than 4 mg/g;the PS2 shale has well-developed laminar structure,larger pore and throat size,better connectivity of pores and throats,high contents of TOC and movable hydrocarbon,S1 of over 4 mg/g,clay content of less than 20%,and porosity of more than 4%;PS3 shale has S1 value higher than 6 mg/g and clay content of 20%-30%,and porosity of less than 4%;and PS4 shale has lower TOC content and low oil content.Shale oil reservoir classification criterion based on five parameters,free hydrocarbon content S1,shale rhythmic structure,clay content,TOC and porosity,was established.The evaluation method of shale oil sweet spot by using the weighted five parameters,and the evaluation index EI were proposed.Through comprehensive analysis,it is concluded that PS2 is best in quality and thus the dual geological and engineering sweet spot of shale oil,PS3 and PS1 come next,the former is more geologic sweet spot,the latter more engineering sweet spot,and PS4 is the poorest.Several vertical and horizontal wells drilled in the PS2 and PS3 sweet spots obtained high oil production.Among them,Well 1701 H has produced stably for 623 days,with cumulative production of over 10000 tons,showing bright exploration prospects of Kong 2 Member shale oil.
文摘In a number of experiments, when detecting particles emitted in beta decays, periodic oscillations of count rate with an amplitude up to tenths of a percent and short bursts vastly exceeding the usual count rate are found. At the same time, several experiments did not detect any differences from the “normal” course of beta decays greater than 0.01%. The article shows that the inconsistency of the experimental results is due to different measurement technique. The assumption is made of the possible participation in the beta decay processes of cosmic slow neutrinos, which makes it possible to explain in a comprehensive manner not only periodic and sporadic changes in the beta decay rate, but also a number of other incomprehensible phenomena associated with beta radioactivity. On the basis of the experiments carried out, an estimate is made of the flux density of slow cosmic neutrinos.
文摘Permian salt-bearing formation covers a substantial area of the Russian Plate.In some places,it is up to several kilometers thick.The salt-bearing formation contains unique accumulations of potassium and magnesium salts,
基金supported by the National Natural Science Foundation of China (Nos.10872068 and 10672057)the Fundamental Research Fund for the Central Universities
文摘The rhythmic movement is a spontaneous behavior due to the central pattern generator （CPG）. At present, the CPG model only shows the spontaneous behavior, but does not refer to the instruction regulation role of the cerebral cortex. In this paper, a modified model based on the Matsuoka neural oscillator theory is presented to better show the regulation role of the cerebral cortex signal to the CPG neuronal network. The complex interaction between the input signal and other parameters in the CPG network is established, making all parameters of the CPG vary with the input signal. In this way, the effect of the input signal to the CPG network is enhanced so that the CPG network can express the self-regulation movement state instead of being limited to the spontaneous behavior, and thus the regulation role of the cerebral cortex signal can be reflected. Numerical simulation shows that the modified model can generate various movement forms with different modes, frequencies, and interchanges between them. It is revealed in theories that the cerebral cortex signal can regulate the mode and frequency of the gait in the ~ourse of the gait movement.
基金Project supported by the National Natural Science Foundation of China (Grant Nos. 61072012, 60901035, 50907044, and 61172009)
文摘Both external and endogenous electrical fields widely exist in the environment of cortical neurons. The effects of a weak alternating current （AC） field on a neural network model with synaptic plasticity are studied. It is found that self-sustained rhythmic firing patterns, which are closely correlated with the cognitive functions, are significantly modified due to the self-organizing of the network in the weak AC field. The activities of the neural networks are affected by the synaptic connection strength, the exterrtal stimuli, and so on. In the presence of learning rules, the synaptic connections can be modulated by the external stimuli, which will further enhance the sensitivity of the network to the external signal. The properties of the external AC stimuli can serve as control parameters in modulating the evolution of the neural network.
文摘We examined theta activity in the temporal hippocampus of ure-thane-chloralose-anesthetized pigs by stimulation with two chemical odors, beta-phenyl ethyl alcohol (PEA;rose-like odor) and n-amyl acetate (banana-like odor). Neural activity was recorded in the neural cell layer nearby fimbria of the temporal hippocampus in three of six animals. Odor stimulation with PEA at a low concentration (10-4 w/w;100 ppm) significantly increased power of the low-frequency theta band (type-2;4 - 6 Hz) for the middle 10 s (w (3, 6, 2.5%) >2.56). The PEA odor at a higher concentration (10-3 w/w;1000 ppm), however, showed a tendency for gradual increase in?the low-frequency theta but the response was insignificant compared with the control. On the other hand, odor stimulation with n-amyl acetate (10-4 w/w;100 ppm) caused no apparent increase or a tendency for decrease in power of the low-frequency theta. Thus, the type-2 theta response in the temporal hippocampus to the PEA odor contrasted strikingly with that to the n-amylacetate in the ure-thane-chloralose-anesthetized pigs. The response of the type-2 theta rhythm in the temporal hippocampus may underlie the difference in emotional sensation and cognition of PEA from n-amyl acetate in the pig.
