为了探讨Rh type C glycoprotein (RHCG)对非小细胞肺癌(non-small cell lung cancer,NSCLC)细胞增殖的影响及可能的作用机制,本研究使用荧光定量PCR法检测12对NSCLC及癌旁组织样本中RHCG mRNA的表达水平及pcDNA3.1-RHCG质粒对A549细胞R...为了探讨Rh type C glycoprotein (RHCG)对非小细胞肺癌(non-small cell lung cancer,NSCLC)细胞增殖的影响及可能的作用机制,本研究使用荧光定量PCR法检测12对NSCLC及癌旁组织样本中RHCG mRNA的表达水平及pcDNA3.1-RHCG质粒对A549细胞RHCG m RNA的表达;采用CCK-8法检测细胞增殖能力;运用PI染色法检测细胞周期;使用免疫印迹法检p-PI3K、PI3K、p-AKT以及AKT蛋白表达水平。本研究发现,与癌旁组织比较,NSCLC中RHCG m RNA表达水平明显降低。RHCG过表达能抑制NSCLC细胞系A549细胞增殖能力。此外,RHCG过表达使A549细胞周期G1/S期转化发生阻滞。本研究还发现,RHCG过表达可下调A549细胞p-PI3K/PI3K和p-AKT/AKT水平。本研究表明,RHCG抑制NSCLC细胞增殖的作用与其抑制PI3K/AKT信号通路有关。展开更多
Objective To compare the effects of recombinant hCG （rhCG） 250 μg and urinary hCG （uhCG） 10 000 1U on triggering oocyte maturation during COH/1VF-ET in Chinese women. Methods A retrospective cohort study which in...Objective To compare the effects of recombinant hCG （rhCG） 250 μg and urinary hCG （uhCG） 10 000 1U on triggering oocyte maturation during COH/1VF-ET in Chinese women. Methods A retrospective cohort study which included COH/1VF-ET cases at reproductive medical center of Peking University Third Hospital from March to May 2010 was performed. Analysis of covariance （ANCOVA） was taken for data analysis. Results There were 318 cycles （group A） using rhCG 250μg and 810 cycles （group B） using uhCG 10 000 1U for triggering. In 1VF procedure, there were no significant differences in the number of oocyte retrieval, 2PN fertilization rate, good-quality embryo rate, embryo implantation rate and clinical pregnancy rate between the two groups. The delivery weeks, mean neonatal birth weight and live birth rates were also similar between the two groups. There was no local allergic reaction after injection or neonatal birth defect in group A. Conclusion The rhCG 250 μg and uhCG 10 000 IU have equal effects on triggering oocyte maturation for patients with normal ovarian function in Chinese population. rhCG may have even lower local discomfort occurrence.展开更多
The structural differences between <em>E. coli</em> AmtB and the human RhCG as well as the Rh50 from <em>Nitrosomonas europaea</em> suggest different ammonia conduction mechanisms for the AmtB ...The structural differences between <em>E. coli</em> AmtB and the human RhCG as well as the Rh50 from <em>Nitrosomonas europaea</em> suggest different ammonia conduction mechanisms for the AmtB and the Rh proteins. This study investigates the mechanism differences by using molecular dynamics simulations on RhCG and Rh50NE structures. Unlike AmtB the Rh proteins lack the aromatic cage at the bottom of the periplasmic vestibule. This report establishes the periplasmic Glu residue as the N<span style="white-space:nowrap;"><span>H</span><sup>+</sup><sub style="margin-left:-10px;">4</sub> </span>binding site for Rh proteins, and the two Phe residues at the entrance of the pore as the NH<sub>3</sub> binding site. The <span style="white-space:nowrap;">N<span>H</span><sup>+</sup><sub style="margin-left:-10px;">4</sub></span> molecule pushed by another ammonium releases one of its protons on its way to the phenyl gate. This study also discovers that, unlike AmtB, the Rh protein pores allow water molecules, which eventually facilitates the NH<sub>3</sub> conduction from periplasm to cytoplasm.展开更多
Background:Previous studies have shown that the interaction of tumor cells and the microenvironment is critical for immunotherapy response.However,predicting clinical response to immunotherapy remains a challenging pr...Background:Previous studies have shown that the interaction of tumor cells and the microenvironment is critical for immunotherapy response.However,predicting clinical response to immunotherapy remains a challenging problem for clinicians.Therefore,this study aimed to investigate the relationship between the expression of Rh family,C glycoprotein(RHCG)in lung adenocarcinoma(LUAD)and prognosis and tumor-infiltrating immune cells.Methods:First,the expression of RHCG in LUAD tissues was detected by biological database.Chi-square test,Kaplan-Meier,univariate and multivariate Cox regression analysis were used to analyze the clinical significance of RHCG expression.To investigate the correlation between RHCG expression and the level of tumor-infiltrating immune cells in LUAD tissues.Receiver operating characteristic analysis was used to compare the predictive power of RHCG with other common immune-related markers.Results:In a TCGA cohort,we found that RHCG was upregulated in LUAD and increased with tumor grade.High RHCG expression was significantly associated with poor clinicopathological features and poor survival,which was further confirmed by clinical correlation analysis and survival analysis results.Furthermore,multivariate analysis indicated that RHCG was an independent prognostic biomarker for LUAD.Significantly,RHCG overexpression was associated with higher infiltration levels of CD4+T cells,CD8+T cells,B cells,macrophages and neutrophils in LUAD.Conclusions:We found that RHCG is upregulated as a biomarker of poor prognosis in LUAD.Importantly,RHCG overexpression correlated with the immune infiltration level of B cells,CD4+T cells,CD8+T cells,macrophages,neutrophils and other immune cells,and was closely related to the overall immune infiltration level of LUAD.These results suggest that RHCG may be a potential biomarker for assessing prognosis and immune infiltration in LUAD.展开更多
文摘为了探讨Rh type C glycoprotein (RHCG)对非小细胞肺癌(non-small cell lung cancer,NSCLC)细胞增殖的影响及可能的作用机制,本研究使用荧光定量PCR法检测12对NSCLC及癌旁组织样本中RHCG mRNA的表达水平及pcDNA3.1-RHCG质粒对A549细胞RHCG m RNA的表达;采用CCK-8法检测细胞增殖能力;运用PI染色法检测细胞周期;使用免疫印迹法检p-PI3K、PI3K、p-AKT以及AKT蛋白表达水平。本研究发现,与癌旁组织比较,NSCLC中RHCG m RNA表达水平明显降低。RHCG过表达能抑制NSCLC细胞系A549细胞增殖能力。此外,RHCG过表达使A549细胞周期G1/S期转化发生阻滞。本研究还发现,RHCG过表达可下调A549细胞p-PI3K/PI3K和p-AKT/AKT水平。本研究表明,RHCG抑制NSCLC细胞增殖的作用与其抑制PI3K/AKT信号通路有关。
文摘Objective To compare the effects of recombinant hCG （rhCG） 250 μg and urinary hCG （uhCG） 10 000 1U on triggering oocyte maturation during COH/1VF-ET in Chinese women. Methods A retrospective cohort study which included COH/1VF-ET cases at reproductive medical center of Peking University Third Hospital from March to May 2010 was performed. Analysis of covariance （ANCOVA） was taken for data analysis. Results There were 318 cycles （group A） using rhCG 250μg and 810 cycles （group B） using uhCG 10 000 1U for triggering. In 1VF procedure, there were no significant differences in the number of oocyte retrieval, 2PN fertilization rate, good-quality embryo rate, embryo implantation rate and clinical pregnancy rate between the two groups. The delivery weeks, mean neonatal birth weight and live birth rates were also similar between the two groups. There was no local allergic reaction after injection or neonatal birth defect in group A. Conclusion The rhCG 250 μg and uhCG 10 000 IU have equal effects on triggering oocyte maturation for patients with normal ovarian function in Chinese population. rhCG may have even lower local discomfort occurrence.
文摘The structural differences between <em>E. coli</em> AmtB and the human RhCG as well as the Rh50 from <em>Nitrosomonas europaea</em> suggest different ammonia conduction mechanisms for the AmtB and the Rh proteins. This study investigates the mechanism differences by using molecular dynamics simulations on RhCG and Rh50NE structures. Unlike AmtB the Rh proteins lack the aromatic cage at the bottom of the periplasmic vestibule. This report establishes the periplasmic Glu residue as the N<span style="white-space:nowrap;"><span>H</span><sup>+</sup><sub style="margin-left:-10px;">4</sub> </span>binding site for Rh proteins, and the two Phe residues at the entrance of the pore as the NH<sub>3</sub> binding site. The <span style="white-space:nowrap;">N<span>H</span><sup>+</sup><sub style="margin-left:-10px;">4</sub></span> molecule pushed by another ammonium releases one of its protons on its way to the phenyl gate. This study also discovers that, unlike AmtB, the Rh protein pores allow water molecules, which eventually facilitates the NH<sub>3</sub> conduction from periplasm to cytoplasm.
文摘Background:Previous studies have shown that the interaction of tumor cells and the microenvironment is critical for immunotherapy response.However,predicting clinical response to immunotherapy remains a challenging problem for clinicians.Therefore,this study aimed to investigate the relationship between the expression of Rh family,C glycoprotein(RHCG)in lung adenocarcinoma(LUAD)and prognosis and tumor-infiltrating immune cells.Methods:First,the expression of RHCG in LUAD tissues was detected by biological database.Chi-square test,Kaplan-Meier,univariate and multivariate Cox regression analysis were used to analyze the clinical significance of RHCG expression.To investigate the correlation between RHCG expression and the level of tumor-infiltrating immune cells in LUAD tissues.Receiver operating characteristic analysis was used to compare the predictive power of RHCG with other common immune-related markers.Results:In a TCGA cohort,we found that RHCG was upregulated in LUAD and increased with tumor grade.High RHCG expression was significantly associated with poor clinicopathological features and poor survival,which was further confirmed by clinical correlation analysis and survival analysis results.Furthermore,multivariate analysis indicated that RHCG was an independent prognostic biomarker for LUAD.Significantly,RHCG overexpression was associated with higher infiltration levels of CD4+T cells,CD8+T cells,B cells,macrophages and neutrophils in LUAD.Conclusions:We found that RHCG is upregulated as a biomarker of poor prognosis in LUAD.Importantly,RHCG overexpression correlated with the immune infiltration level of B cells,CD4+T cells,CD8+T cells,macrophages,neutrophils and other immune cells,and was closely related to the overall immune infiltration level of LUAD.These results suggest that RHCG may be a potential biomarker for assessing prognosis and immune infiltration in LUAD.
