目的:探讨脑慢性低灌注白质损伤后大鼠脑周细胞RGS5(Regulators of G protein signaling 5,RGS5)、PDGFR-β(Platelet-derived growth factor receptor-beta,PDGFR-β)的表达。方法:采用蛋白免疫印迹法检测脑慢性低灌注白质损伤大鼠脑...目的:探讨脑慢性低灌注白质损伤后大鼠脑周细胞RGS5(Regulators of G protein signaling 5,RGS5)、PDGFR-β(Platelet-derived growth factor receptor-beta,PDGFR-β)的表达。方法:采用蛋白免疫印迹法检测脑慢性低灌注白质损伤大鼠脑周细胞RGS5、PDGFR-β在不同时间段(术后第7、14、30、60d)表达变化。结果:(1)RGS5蛋白在对照组中有稳定的表达,与对照组相比,模型组在术后第14d明显下调(P<0.05),第30d表达增强明显(P<0.05),第60d达到高峰(P<0.05);(2)PDGFR-β蛋白在对照组中也有稳定的表达,与对照组相比,模型组在术后第30d表达至高峰(P<0.01),第60d有所下降,但仍高于正常(P<0.05)。结论:正常生理条件下,脑周细胞中有一定量的RGS5和PDGFR-β表达;脑慢性低灌注白质损伤后期阶段,脑周细胞中RGS5和PDGFR-β蛋白表达增强,且PDGFR-β先于RGS5达到表达高峰,两者表达存在时相关系。展开更多
AIM:To explore the regulator of G-protein signaling 5 (RGS5) expression in gastric carcinoma and its association with differentiation and microvascular density (MVD).METHODS:Expression of RGS5 and CD34 were examined i...AIM:To explore the regulator of G-protein signaling 5 (RGS5) expression in gastric carcinoma and its association with differentiation and microvascular density (MVD).METHODS:Expression of RGS5 and CD34 were examined in 76 cases of gastric carcinoma,including 22 cases with lymph node metastasis and 54 cases without lymph node metastasis determined by immunohistochemistry (IHC).MVD was assessed using CD34 monoclonal antibody.The presence of RGS5 and CD34 was analyzed by IHC using the Envision technique.RESULTS:The RGS5 expression in gastric carcinoma was positively correlated with the differentiation of the tumor (r=0.345,P < 0.001),but not related with age,gender,tumor size,clinical stage and lymph node metastasis (P > 0.05).The average MVD in the group with lymph node metastasis was significantly higher than that in the group without lymph node metastasis (P < 0.05).RGS5 expression was negatively correlated with the average MVD (P < 0.05).CONCLUSION:RGS5 expression level in gastric carcinoma is associated with the differentiation and MVD of the tumor,and may be used as an important parameter for determining the prognosis of gastric carcinoma patients.展开更多
目的：研究人参皂苷Rg5对胃癌BGC-823细胞侵袭迁移的影响,并通过微小RNA-125b（microRNA-125b,miR-125b）/类固醇敏感性调节蛋白相关脂类转运体结构域13（St AR-related lipid transfer domain protein 13,STARD13）/唾液酸酶1（neurami...目的：研究人参皂苷Rg5对胃癌BGC-823细胞侵袭迁移的影响,并通过微小RNA-125b（microRNA-125b,miR-125b）/类固醇敏感性调节蛋白相关脂类转运体结构域13（St AR-related lipid transfer domain protein 13,STARD13）/唾液酸酶1（neuraminidase 1,NEU1）信号通路探讨人参皂苷Rg5抑制胃癌侵袭迁移的作用机制。方法：人参皂苷Rg5（12.5,25,50μmol·L-1）处理BGC-823细胞后,采用transwell小室实验检测人参皂苷Rg5对BGC-823细胞侵袭、迁移能力的影响,实时荧光定量PCR（Real-time PCR）检测miR-125b,STARD13,NEU1 mRNA的表达,蛋白免疫印迹法（Western blot）检测STARD13,NEU1蛋白的表达。结果：人参皂苷Rg5（25,50μmol·L-1）可有效抑制BGC-823细胞的侵袭、迁移。miR-125b,STARD13,NEU1 mRNA的表达,与空白组比较,人参皂苷Rg512.5μmol·L-1组的miR-125b相对表达水平降低,STARD13,NEU1 mRNA相对表达水平升高,但无统计学差异;人参皂苷Rg5（25,50μmol·L-1）组miR-125b相对表达水平降低,STARD13,NEU1 mRNA相对表达水平增高（P〈0.01）。STARD13,NEU1蛋白的表达,与空白组比较,人参皂苷Rg5（12.5,25μmol·L-1）组的STARD13蛋白相对表达水平升高,人参皂苷Rg5（12.5μmol·L-1）组的NEU1蛋白相对表达水平升高,但差异无统计学意义;人参皂苷Rg5（50μmol·L-1）组的STARD13蛋白相对表达水平升高（P〈0.05）,人参皂苷Rg5（25,50μmol·L-1）组NEU1蛋白相对表达水平升高（P〈0.01）。结论：人参皂苷Rg5可能通过降低miR-125b的表达,上调miR-125b靶基因STARD13,NEU1的表达,抑制胃癌BGC-823细胞的侵袭、迁移。展开更多
文摘目的:探讨脑慢性低灌注白质损伤后大鼠脑周细胞RGS5(Regulators of G protein signaling 5,RGS5)、PDGFR-β(Platelet-derived growth factor receptor-beta,PDGFR-β)的表达。方法:采用蛋白免疫印迹法检测脑慢性低灌注白质损伤大鼠脑周细胞RGS5、PDGFR-β在不同时间段(术后第7、14、30、60d)表达变化。结果:(1)RGS5蛋白在对照组中有稳定的表达,与对照组相比,模型组在术后第14d明显下调(P<0.05),第30d表达增强明显(P<0.05),第60d达到高峰(P<0.05);(2)PDGFR-β蛋白在对照组中也有稳定的表达,与对照组相比,模型组在术后第30d表达至高峰(P<0.01),第60d有所下降,但仍高于正常(P<0.05)。结论:正常生理条件下,脑周细胞中有一定量的RGS5和PDGFR-β表达;脑慢性低灌注白质损伤后期阶段,脑周细胞中RGS5和PDGFR-β蛋白表达增强,且PDGFR-β先于RGS5达到表达高峰,两者表达存在时相关系。
基金Supported by Grants from National Natural Science Foundation of China,No.30870962
文摘AIM:To explore the regulator of G-protein signaling 5 (RGS5) expression in gastric carcinoma and its association with differentiation and microvascular density (MVD).METHODS:Expression of RGS5 and CD34 were examined in 76 cases of gastric carcinoma,including 22 cases with lymph node metastasis and 54 cases without lymph node metastasis determined by immunohistochemistry (IHC).MVD was assessed using CD34 monoclonal antibody.The presence of RGS5 and CD34 was analyzed by IHC using the Envision technique.RESULTS:The RGS5 expression in gastric carcinoma was positively correlated with the differentiation of the tumor (r=0.345,P < 0.001),but not related with age,gender,tumor size,clinical stage and lymph node metastasis (P > 0.05).The average MVD in the group with lymph node metastasis was significantly higher than that in the group without lymph node metastasis (P < 0.05).RGS5 expression was negatively correlated with the average MVD (P < 0.05).CONCLUSION:RGS5 expression level in gastric carcinoma is associated with the differentiation and MVD of the tumor,and may be used as an important parameter for determining the prognosis of gastric carcinoma patients.
