目的:本研究旨在评价人参皂苷Rg1对AA的保护作用,并进一步探讨其机制。方法:本研究通过注射环磷酰胺(CTX)建立骨髓抑制小鼠模型。CTX + Rg1组小鼠Rg1灌胃13 d。HE检测骨髓、胸腺和脾脏的病理变化。取小鼠眼眶血进行血常规检查。然后用...目的:本研究旨在评价人参皂苷Rg1对AA的保护作用,并进一步探讨其机制。方法:本研究通过注射环磷酰胺(CTX)建立骨髓抑制小鼠模型。CTX + Rg1组小鼠Rg1灌胃13 d。HE检测骨髓、胸腺和脾脏的病理变化。取小鼠眼眶血进行血常规检查。然后用流式细胞仪检测骨髓细胞的比例。通过WB检测MAPK通路中p-p38、p38、p-JNK、JNK、p-ERK、ERK的表达。结果:病理检查显示,CTX严重破坏小鼠骨髓、胸腺和脾脏的结构特征,降低骨髓造血细胞的比例。Rg1可通过抑制MAPK信号通路显著减轻小鼠骨髓抑制。结论:本研究提示人参皂苷Rg1通过MAPK信号通路缓解骨髓抑制作用,对AA有一定的治疗作用。Objective: This study was designed to evaluate the protective effects of ginsenoside Rg1 on AA and further investigate the underlying mechanism. Methods: In this study, Cyclophosphamide (CTX) was injected to establish the myelosuppression mouse model. The mice in the CTX + Rg1 group were treated with Rg1 for 13 days. The pathological changes of bone marrow, thymus, and spleen were detected through HE (Hematoxylin-Eosin St). Orbital blood of mice was collected for blood routine examination. Afterwards, the proportions of bone marrow cells were evaluated by flow cytometry assay. MAPK pathway was detected via WB for the expressions of p-p38, p38, p-JNK, JNK, p-ERK, and ERK. Results: Pathological examination revealed that CTX severely damaged the structural features of the bone marrow, thymus, and spleens, and decreased the proportion of hematopoietic cells in the bone marrow of mice. Treatment of Rg1 significantly alleviated myelosuppression in mice by inhibiting MAPK signaling pathway. Conclusion: This study suggested that ginsenoside Rg1 treated AA by alleviating myelosuppression through MAPK signaling pathway.展开更多
Acute kidney injury is a common complication that can arise from improper medication use,mechanical injury,and other underlying conditions,potentially leading to life-threatening situations in severe cases.Ginsenoside...Acute kidney injury is a common complication that can arise from improper medication use,mechanical injury,and other underlying conditions,potentially leading to life-threatening situations in severe cases.Ginsenoside Rg1,a major protopanaxatriol saponin,exhibits pharmacological activities such as antioxidant,anti-inflammatory,and anti-tumor effects,demonstrating significant nephroprotective properties in various renal injury models.This article reviews the mechanisms and recent research advancements of ginsenoside Rg1 in preventing acute kidney injury,focusing on five key areas:antioxidant,anti-inflammatory,anti-apoptotic,anti-podocyte autophagy,and anti-fibrotic activities.These insights provide a valuable foundation for its further development and application.展开更多
In study,we investigated the effect of treatment with combination of walnut peptides with molecular weight<3 kDa and ginsenoside Rg1(<3 kDa+Rg1)on scopolamine-induced cognitive impairment in mice and the mechani...In study,we investigated the effect of treatment with combination of walnut peptides with molecular weight<3 kDa and ginsenoside Rg1(<3 kDa+Rg1)on scopolamine-induced cognitive impairment in mice and the mechanism of brain-derived neurotrophic factor(BDNF)/tyrosine kinase B(TrKB)/cAMP response element-binding protein(CREB)signaling pathway in PC12 cells.In behavioral experiments,<3 kDa+Rg1 treatment improved the memorizing ability of mice.Treatment with<3 kDa+Rg1 significantly regulated the function of neurotransmitters and effectively improved the morphology of the neurons determined by hematoxylin and eosin(H&E),Nissl,and Golgi staining.Additionally,immunohistochemistry showed that the<3 kDa+Rg1 treatment significantly decreased acetylcholinesterase(AChE)activity and increased choline acetyl transferase(ChAT)content in the hippocampus.The treatment upregulated vesicular acetylcholine transporter(VAChT),activated the BDNF/TrKB/CREB signaling pathway,improved the remodeling of dendritic spines,and enhanced cholinergic functions.In the scopolamine-induced PC12 cells,combination treatment increased thioredoxin-1(Trx-1)expression after administering TrKB and activated signaling pathway.The results showed combination of<3 kDa+Rg1 activated the BDNF/TrKB/CREB signaling pathway by regulating function of neurotransmitters and enhanced cholinergic function to decrease cognitive impairment.展开更多
文摘目的:本研究旨在评价人参皂苷Rg1对AA的保护作用,并进一步探讨其机制。方法:本研究通过注射环磷酰胺(CTX)建立骨髓抑制小鼠模型。CTX + Rg1组小鼠Rg1灌胃13 d。HE检测骨髓、胸腺和脾脏的病理变化。取小鼠眼眶血进行血常规检查。然后用流式细胞仪检测骨髓细胞的比例。通过WB检测MAPK通路中p-p38、p38、p-JNK、JNK、p-ERK、ERK的表达。结果:病理检查显示,CTX严重破坏小鼠骨髓、胸腺和脾脏的结构特征,降低骨髓造血细胞的比例。Rg1可通过抑制MAPK信号通路显著减轻小鼠骨髓抑制。结论:本研究提示人参皂苷Rg1通过MAPK信号通路缓解骨髓抑制作用,对AA有一定的治疗作用。Objective: This study was designed to evaluate the protective effects of ginsenoside Rg1 on AA and further investigate the underlying mechanism. Methods: In this study, Cyclophosphamide (CTX) was injected to establish the myelosuppression mouse model. The mice in the CTX + Rg1 group were treated with Rg1 for 13 days. The pathological changes of bone marrow, thymus, and spleen were detected through HE (Hematoxylin-Eosin St). Orbital blood of mice was collected for blood routine examination. Afterwards, the proportions of bone marrow cells were evaluated by flow cytometry assay. MAPK pathway was detected via WB for the expressions of p-p38, p38, p-JNK, JNK, p-ERK, and ERK. Results: Pathological examination revealed that CTX severely damaged the structural features of the bone marrow, thymus, and spleens, and decreased the proportion of hematopoietic cells in the bone marrow of mice. Treatment of Rg1 significantly alleviated myelosuppression in mice by inhibiting MAPK signaling pathway. Conclusion: This study suggested that ginsenoside Rg1 treated AA by alleviating myelosuppression through MAPK signaling pathway.
基金supported by the National Natural Science Foundation of China(82260812)Youth program of National Natural Science Foundation of China(81803838)+8 种基金Regional fund project of National Natural Science Foundation of China(82060754)Science and technology project of Guizhou health and Health Committee(No.gzwkj 2021-441)Guizhou Provincial Special research project on science and technology of traditional Chinese medicine and ethnic medicine(QZYY-2021-035)Guizhou Province Youth Science and Technology Talent Plan(YQK[2023]038)Guizhou Province Traditional Chinese Medicine and Ethnic Medicine 2011 Collaborative Innovation Center([2022]026)the Guizhou Provincial Department of health outstanding young medical talents fund([2021]3)Key project at central government level:The ability establishment of sustainable use for valuable Chinese medicine resources(2060302)Science and Technology Department of Zunyi city([2022]419,[2022]420)Postdoctoral initiation program([2020]-044).
文摘Acute kidney injury is a common complication that can arise from improper medication use,mechanical injury,and other underlying conditions,potentially leading to life-threatening situations in severe cases.Ginsenoside Rg1,a major protopanaxatriol saponin,exhibits pharmacological activities such as antioxidant,anti-inflammatory,and anti-tumor effects,demonstrating significant nephroprotective properties in various renal injury models.This article reviews the mechanisms and recent research advancements of ginsenoside Rg1 in preventing acute kidney injury,focusing on five key areas:antioxidant,anti-inflammatory,anti-apoptotic,anti-podocyte autophagy,and anti-fibrotic activities.These insights provide a valuable foundation for its further development and application.
基金funded by the Research and Product Development of Key Technologies for Efficient Utilization of Walnut Meal(20210203125SF)Changchun 2021 Local Transformation of Scientific and Technological Achievements Guide Special(21KY05).
文摘In study,we investigated the effect of treatment with combination of walnut peptides with molecular weight<3 kDa and ginsenoside Rg1(<3 kDa+Rg1)on scopolamine-induced cognitive impairment in mice and the mechanism of brain-derived neurotrophic factor(BDNF)/tyrosine kinase B(TrKB)/cAMP response element-binding protein(CREB)signaling pathway in PC12 cells.In behavioral experiments,<3 kDa+Rg1 treatment improved the memorizing ability of mice.Treatment with<3 kDa+Rg1 significantly regulated the function of neurotransmitters and effectively improved the morphology of the neurons determined by hematoxylin and eosin(H&E),Nissl,and Golgi staining.Additionally,immunohistochemistry showed that the<3 kDa+Rg1 treatment significantly decreased acetylcholinesterase(AChE)activity and increased choline acetyl transferase(ChAT)content in the hippocampus.The treatment upregulated vesicular acetylcholine transporter(VAChT),activated the BDNF/TrKB/CREB signaling pathway,improved the remodeling of dendritic spines,and enhanced cholinergic functions.In the scopolamine-induced PC12 cells,combination treatment increased thioredoxin-1(Trx-1)expression after administering TrKB and activated signaling pathway.The results showed combination of<3 kDa+Rg1 activated the BDNF/TrKB/CREB signaling pathway by regulating function of neurotransmitters and enhanced cholinergic function to decrease cognitive impairment.
