针对以往船舶备件需求预测精度不高,无法满足船舶综合保障的实际问题,文章建立一种基于改进红狐优化算法(improved red fox optimization,IRFO)的支持向量机回归(support vector regression,SVR)的船舶备件预测模型。为进一步提高红狐...针对以往船舶备件需求预测精度不高,无法满足船舶综合保障的实际问题,文章建立一种基于改进红狐优化算法(improved red fox optimization,IRFO)的支持向量机回归(support vector regression,SVR)的船舶备件预测模型。为进一步提高红狐优化算法(red fox optimization,RFO)的寻优精度,重构其全局搜索公式,并融合精英反向学习策略。采用基准测试函数对IRFO算法进行仿真实验,实验表明,IRFO算法比RFO算法、粒子群算法、灰狼优化算法寻优能力更强,综合性能更优。基于船舶备件历史数据,建立IRFO-SVR船舶备件预测模型,通过对比其他模型的预测结果,表明IRFO-SVR的预测效果更佳。展开更多
Potato(Solanum tuberosum L.) is the fourth largest food crop in the world. Low temperatures cause serious damage to potato plants every year, and freezing tolerance has become a hot spot in potato research. Galactinol...Potato(Solanum tuberosum L.) is the fourth largest food crop in the world. Low temperatures cause serious damage to potato plants every year, and freezing tolerance has become a hot spot in potato research. Galactinol synthase(GolS) is a key enzyme in the synthesis of raffinose family oligosaccharides(RFOs), and plays an important role in the response of plants to abiotic stress. In this study, the ScGolS1 gene from Solanum commersonii was cloned and introduced into the S. tuberosum cultivars 'Atlantic' and 'Desiree' via Agrobacterium-mediated transformation. Phenotyping assays showed that overexpression of ScGolS1 could significantly improve freezing tolerance in transgenic potato plants.Further physiological and biochemical experiments showed that the transgenic lines had lower relative conductivity, malondialdehyde content,and 3,3-diaminobenzidine staining and a higher plant survival rate compared with wild type(WT) under cold stress. Moreover, the C-repeat binding factors(CBF1, CBF2 and CBF3), the downstream cold-responsive genes COR413 and COR47, and the ethylene-responsive factor(ERF)transcription factor genes ERF3, ERF4 and ERF6, which function in the ethylene signaling pathway, were all induced by freezing treatment and expressed at higher levels in the ScGolS1 overexpression lines compared with WT. Besides, the expression of some genes such as MIPS, STS and RS from the RFO metabolic pathway was up-regulated under cold stress, resulting in changes in the content of some soluble sugars. This indicated that ScGolS1 overexpression altered the sugar composition and enhanced freezing tolerance in transgenic potato by inducing the ethylene and CBF signaling pathways. These results provided theoretical support and genetic resources for freezing tolerance breeding in potato.展开更多
文摘针对以往船舶备件需求预测精度不高,无法满足船舶综合保障的实际问题,文章建立一种基于改进红狐优化算法(improved red fox optimization,IRFO)的支持向量机回归(support vector regression,SVR)的船舶备件预测模型。为进一步提高红狐优化算法(red fox optimization,RFO)的寻优精度,重构其全局搜索公式,并融合精英反向学习策略。采用基准测试函数对IRFO算法进行仿真实验,实验表明,IRFO算法比RFO算法、粒子群算法、灰狼优化算法寻优能力更强,综合性能更优。基于船舶备件历史数据,建立IRFO-SVR船舶备件预测模型,通过对比其他模型的预测结果,表明IRFO-SVR的预测效果更佳。
基金supported by the National Natural Science Foundation of China (Grant No. 31561143006)China Agriculture Research System (Grant No. CARS-9)the Chinese Academy of Agricultural Sciences Innovation Project (Grant No. CAASASTIP-IVFCAAS)。
文摘Potato(Solanum tuberosum L.) is the fourth largest food crop in the world. Low temperatures cause serious damage to potato plants every year, and freezing tolerance has become a hot spot in potato research. Galactinol synthase(GolS) is a key enzyme in the synthesis of raffinose family oligosaccharides(RFOs), and plays an important role in the response of plants to abiotic stress. In this study, the ScGolS1 gene from Solanum commersonii was cloned and introduced into the S. tuberosum cultivars 'Atlantic' and 'Desiree' via Agrobacterium-mediated transformation. Phenotyping assays showed that overexpression of ScGolS1 could significantly improve freezing tolerance in transgenic potato plants.Further physiological and biochemical experiments showed that the transgenic lines had lower relative conductivity, malondialdehyde content,and 3,3-diaminobenzidine staining and a higher plant survival rate compared with wild type(WT) under cold stress. Moreover, the C-repeat binding factors(CBF1, CBF2 and CBF3), the downstream cold-responsive genes COR413 and COR47, and the ethylene-responsive factor(ERF)transcription factor genes ERF3, ERF4 and ERF6, which function in the ethylene signaling pathway, were all induced by freezing treatment and expressed at higher levels in the ScGolS1 overexpression lines compared with WT. Besides, the expression of some genes such as MIPS, STS and RS from the RFO metabolic pathway was up-regulated under cold stress, resulting in changes in the content of some soluble sugars. This indicated that ScGolS1 overexpression altered the sugar composition and enhanced freezing tolerance in transgenic potato by inducing the ethylene and CBF signaling pathways. These results provided theoretical support and genetic resources for freezing tolerance breeding in potato.
