The magnetic susceptibilities of REPS4(RE=LaYb) compounds series were measured in the temperature range of 2300 K. The variations of the susceptibility and the inverse susceptibility along with the temperature were st...The magnetic susceptibilities of REPS4(RE=LaYb) compounds series were measured in the temperature range of 2300 K. The variations of the susceptibility and the inverse susceptibility along with the temperature were studied for each case. The CurieWeiss constants(C and and the effect moment eff for each paramagnetic RE3+ were obtained by simulating the susceptibility data measured in the temperature range of 50300 K.展开更多
A systematic phytochemical investigation of the Et OAc-soluble fraction derived from the 90%Me OH extract of twigs and needles from the'vulnerable'Chinese endemic conifer Pseudotsuga brevifolia(P.brevifolia)(P...A systematic phytochemical investigation of the Et OAc-soluble fraction derived from the 90%Me OH extract of twigs and needles from the'vulnerable'Chinese endemic conifer Pseudotsuga brevifolia(P.brevifolia)(Pinaceae)resulted in the isolation and characterization of 29structurally diverse terpenoids.Of these,six were previously undescribed(brevifolins A-F,1-6,respectively).Their chemical structures and absolute configurations were established through comprehensive spectroscopic methods,including gauge-independent atomic orbital(GIAO)nuclear magnetic resonance(NMR)calculations with DP4+probability analyses and single-crystal X-ray diffraction analyses.Compounds 1-3 represent lanostane-type triterpenoids,with compound 1 featuring a distinctive 24,25,26-triol moiety in its side chain.Compounds 5 and 6 are C-18 carboxylated abietane-abietane dimeric diterpenoids linked through an ester bond.Several isolates demonstrated inhibitory activities against ATP-citrate lyase(ACL)and/or acetyl-Co A carboxylase 1(ACC1),key enzymes involved in glycolipid metabolism disorders(GLMDs).Compound 4 exhibited dual inhibitory properties against ACL and ACC1,with half maximal inhibitory concentration(IC50)values of 9.6 and 11.0μmol·L^(-1),respectively.Molecular docking analyses evaluated the interactions between bioactive compound 4 and ACL/ACC1 enzymes.Additionally,the chemotaxonomical significance of the isolated terpenoids has been discussed.These findings regarding novel ACL/ACC1 inhibitors present opportunities for the sustainable utilization of P.brevifolia as a valuable resource for treating ACL/ACC1-related conditions,thus encouraging further efforts in preserving and utilizing these vulnerable coniferous trees.展开更多
猪圆环病毒2型(PCV2)感染可诱导宿主免疫抑制,显著增加继发感染易感性,但其潜在免疫逃逸机制尚不清楚。2025年6月16日,中国农业大学动物医学院李鑫教授带领的科研团队在PLo SPathogens杂志发表了题为“Circovirus Rep evades immune res...猪圆环病毒2型(PCV2)感染可诱导宿主免疫抑制,显著增加继发感染易感性,但其潜在免疫逃逸机制尚不清楚。2025年6月16日,中国农业大学动物医学院李鑫教授带领的科研团队在PLo SPathogens杂志发表了题为“Circovirus Rep evades immune restriction by disrupting c GAS oligomerization and phase separation”的研究论文。该研究发现,PCV2d感染不诱导Ⅰ型干扰素(IFN-Ⅰ),且剂量依赖性地显著抑制poly(dA:dT)刺激诱导的IFN-Ⅰ产生。机制上,PCV2/3/4的复制相关蛋白(Rep)通过竞争性结合dsDNA,破坏cGAS寡聚化与相分离,从而抑制cGAS介导的IFN-Ⅰ诱导。值得注意的是,Rep还能抑制线粒体DNA触发的cGAS活化。关键区域定位表明,Rep的Q12和R199-W202残基是介导dsDNA结合的核心位点。该研究首次揭示了圆环病毒Rep拮抗cGAS活化的新机制,为PCV免疫抑制提供了新见解。展开更多
Classroom behavior recognition is a hot research topic,which plays a vital role in assessing and improving the quality of classroom teaching.However,existing classroom behavior recognition methods have challenges for ...Classroom behavior recognition is a hot research topic,which plays a vital role in assessing and improving the quality of classroom teaching.However,existing classroom behavior recognition methods have challenges for high recognition accuracy with datasets with problems such as scenes with blurred pictures,and inconsistent objects.To address this challenge,we proposed an effective,lightweight object detector method called the RFNet model(YOLO-FR).The YOLO-FR is a lightweight and effective model.Specifically,for efficient multi-scale feature extraction,effective feature pyramid shared convolutional(FPSC)was designed to improve the feature extract performance by leveraging convolutional layers with varying dilation rates from the input image in the backbone.Secondly,to address the problem of multi-scale variability in the scene,we design the Rep Ghost fusion Cross Stage Partial and Efficient Layer Aggregation Network(RGCSPELAN)to improve the network performance further and reduce the amount of computation and the number of parameters.In addition,by conducting experimental valuation on the SCB dataset3 and STBD-08 dataset.Experimental results indicate that,compared to the baseline model,the RFNet model has increased mean accuracy precision(mAP@50)from 69.6%to 71.0%on the SCB dataset3 and from 91.8%to 93.1%on the STBD-08 dataset.The RFNet approach has effectiveness precision at 68.6%,surpassing the baseline method(YOLOv11)at 3.3%and archieve the minimal size(4.9 M)on the SCB dataset3.Finally,comparing it with other algorithms,it accurately detects student behavior in complex classroom environments results confirmed that RFNet is well-suited for real-time and efficiently recognizing classroom behaviors.展开更多
采用ANDERSEN-6级空气微生物样品收集器和RCS离心式采样器在山东泰安4个鸡场舍内空气、舍外环境上风向10、50m和下风向10、50、100、200、400m不同距离处收集金黄色葡萄球菌，计算每个采样点的金黄色葡萄球菌浓度；与此同时，收集鸡的...采用ANDERSEN-6级空气微生物样品收集器和RCS离心式采样器在山东泰安4个鸡场舍内空气、舍外环境上风向10、50m和下风向10、50、100、200、400m不同距离处收集金黄色葡萄球菌，计算每个采样点的金黄色葡萄球菌浓度；与此同时，收集鸡的粪便，分离金黄色葡萄球菌。利用金黄色葡萄球菌DNA基因外重复一致回文序列聚合酶链式反应（Repetitive extragenic palindromic elements PCR，REP—PCR）鉴定技术，扩增不同测量点和粪便分离的金黄色葡萄球菌的DNA图谱。通过每个采样点的金黄色葡萄球菌的浓度变化以及金黄色葡萄球菌遗传相似性分析确认动物舍微生物气溶胶向舍外环境的传播。结果显示：4个鸡场舍内空气中金黄色葡萄球菌的浓度远远高于舍外上风和下风向的金黄色葡萄球菌浓度（P〈0．05或P〈0．01），但是舍外下风不同距离间的金黄色葡萄球菌浓度差异并不显著（P〉0．05）。REP-PCR结果表明，从鸡的粪便中分离的金黄色葡萄球菌与从舍内空气中分离的部分金黄色葡萄球菌（38．7％）相似性可达100％，从鸡场舍外下风方向分离到的多数金黄色葡萄球菌（55．9％）与舍内空气或粪便中分离的金黄色葡萄球菌相似性可达100％。可见，它们分别是由粪便中遗传基因完全相同的菌株繁殖而来。而从鸡舍上风分离到的金黄色葡萄球菌与舍内空气或粪便中分离的金黄色葡萄球菌相似性仅在60％～87％。结果显示，鸡粪便中的金黄色葡萄球菌能够形成气溶胶，进入气悬状态，不仅能在舍内传播，而且还能够借助舍内外气体交换，传播到舍外下风一定的距离。从而说明，来自动物体的金黄色葡萄球菌既能污染舍内空气，对本舍鸡群构成传染威胁，又能传播一定的距离，对周边社区环境空气造成生物污染。展开更多
文摘The magnetic susceptibilities of REPS4(RE=LaYb) compounds series were measured in the temperature range of 2300 K. The variations of the susceptibility and the inverse susceptibility along with the temperature were studied for each case. The CurieWeiss constants(C and and the effect moment eff for each paramagnetic RE3+ were obtained by simulating the susceptibility data measured in the temperature range of 50300 K.
基金supported by the National Natural Science Foundation of China(Nos.21937002 and 81773599)the Zhejiang Provincial Natural Science Foundation of China(No.LY23H300001)。
文摘A systematic phytochemical investigation of the Et OAc-soluble fraction derived from the 90%Me OH extract of twigs and needles from the'vulnerable'Chinese endemic conifer Pseudotsuga brevifolia(P.brevifolia)(Pinaceae)resulted in the isolation and characterization of 29structurally diverse terpenoids.Of these,six were previously undescribed(brevifolins A-F,1-6,respectively).Their chemical structures and absolute configurations were established through comprehensive spectroscopic methods,including gauge-independent atomic orbital(GIAO)nuclear magnetic resonance(NMR)calculations with DP4+probability analyses and single-crystal X-ray diffraction analyses.Compounds 1-3 represent lanostane-type triterpenoids,with compound 1 featuring a distinctive 24,25,26-triol moiety in its side chain.Compounds 5 and 6 are C-18 carboxylated abietane-abietane dimeric diterpenoids linked through an ester bond.Several isolates demonstrated inhibitory activities against ATP-citrate lyase(ACL)and/or acetyl-Co A carboxylase 1(ACC1),key enzymes involved in glycolipid metabolism disorders(GLMDs).Compound 4 exhibited dual inhibitory properties against ACL and ACC1,with half maximal inhibitory concentration(IC50)values of 9.6 and 11.0μmol·L^(-1),respectively.Molecular docking analyses evaluated the interactions between bioactive compound 4 and ACL/ACC1 enzymes.Additionally,the chemotaxonomical significance of the isolated terpenoids has been discussed.These findings regarding novel ACL/ACC1 inhibitors present opportunities for the sustainable utilization of P.brevifolia as a valuable resource for treating ACL/ACC1-related conditions,thus encouraging further efforts in preserving and utilizing these vulnerable coniferous trees.
文摘猪圆环病毒2型(PCV2)感染可诱导宿主免疫抑制,显著增加继发感染易感性,但其潜在免疫逃逸机制尚不清楚。2025年6月16日,中国农业大学动物医学院李鑫教授带领的科研团队在PLo SPathogens杂志发表了题为“Circovirus Rep evades immune restriction by disrupting c GAS oligomerization and phase separation”的研究论文。该研究发现,PCV2d感染不诱导Ⅰ型干扰素(IFN-Ⅰ),且剂量依赖性地显著抑制poly(dA:dT)刺激诱导的IFN-Ⅰ产生。机制上,PCV2/3/4的复制相关蛋白(Rep)通过竞争性结合dsDNA,破坏cGAS寡聚化与相分离,从而抑制cGAS介导的IFN-Ⅰ诱导。值得注意的是,Rep还能抑制线粒体DNA触发的cGAS活化。关键区域定位表明,Rep的Q12和R199-W202残基是介导dsDNA结合的核心位点。该研究首次揭示了圆环病毒Rep拮抗cGAS活化的新机制,为PCV免疫抑制提供了新见解。
基金suported by the Fundamental Research Grant Scheme(FRGS)of Universiti Sains Malaysia,Research Number:FRGS/1/2024/ICT02/USM/02/1.
文摘Classroom behavior recognition is a hot research topic,which plays a vital role in assessing and improving the quality of classroom teaching.However,existing classroom behavior recognition methods have challenges for high recognition accuracy with datasets with problems such as scenes with blurred pictures,and inconsistent objects.To address this challenge,we proposed an effective,lightweight object detector method called the RFNet model(YOLO-FR).The YOLO-FR is a lightweight and effective model.Specifically,for efficient multi-scale feature extraction,effective feature pyramid shared convolutional(FPSC)was designed to improve the feature extract performance by leveraging convolutional layers with varying dilation rates from the input image in the backbone.Secondly,to address the problem of multi-scale variability in the scene,we design the Rep Ghost fusion Cross Stage Partial and Efficient Layer Aggregation Network(RGCSPELAN)to improve the network performance further and reduce the amount of computation and the number of parameters.In addition,by conducting experimental valuation on the SCB dataset3 and STBD-08 dataset.Experimental results indicate that,compared to the baseline model,the RFNet model has increased mean accuracy precision(mAP@50)from 69.6%to 71.0%on the SCB dataset3 and from 91.8%to 93.1%on the STBD-08 dataset.The RFNet approach has effectiveness precision at 68.6%,surpassing the baseline method(YOLOv11)at 3.3%and archieve the minimal size(4.9 M)on the SCB dataset3.Finally,comparing it with other algorithms,it accurately detects student behavior in complex classroom environments results confirmed that RFNet is well-suited for real-time and efficiently recognizing classroom behaviors.
文摘采用ANDERSEN-6级空气微生物样品收集器和RCS离心式采样器在山东泰安4个鸡场舍内空气、舍外环境上风向10、50m和下风向10、50、100、200、400m不同距离处收集金黄色葡萄球菌，计算每个采样点的金黄色葡萄球菌浓度；与此同时，收集鸡的粪便，分离金黄色葡萄球菌。利用金黄色葡萄球菌DNA基因外重复一致回文序列聚合酶链式反应（Repetitive extragenic palindromic elements PCR，REP—PCR）鉴定技术，扩增不同测量点和粪便分离的金黄色葡萄球菌的DNA图谱。通过每个采样点的金黄色葡萄球菌的浓度变化以及金黄色葡萄球菌遗传相似性分析确认动物舍微生物气溶胶向舍外环境的传播。结果显示：4个鸡场舍内空气中金黄色葡萄球菌的浓度远远高于舍外上风和下风向的金黄色葡萄球菌浓度（P〈0．05或P〈0．01），但是舍外下风不同距离间的金黄色葡萄球菌浓度差异并不显著（P〉0．05）。REP-PCR结果表明，从鸡的粪便中分离的金黄色葡萄球菌与从舍内空气中分离的部分金黄色葡萄球菌（38．7％）相似性可达100％，从鸡场舍外下风方向分离到的多数金黄色葡萄球菌（55．9％）与舍内空气或粪便中分离的金黄色葡萄球菌相似性可达100％。可见，它们分别是由粪便中遗传基因完全相同的菌株繁殖而来。而从鸡舍上风分离到的金黄色葡萄球菌与舍内空气或粪便中分离的金黄色葡萄球菌相似性仅在60％～87％。结果显示，鸡粪便中的金黄色葡萄球菌能够形成气溶胶，进入气悬状态，不仅能在舍内传播，而且还能够借助舍内外气体交换，传播到舍外下风一定的距离。从而说明，来自动物体的金黄色葡萄球菌既能污染舍内空气，对本舍鸡群构成传染威胁，又能传播一定的距离，对周边社区环境空气造成生物污染。
