Apple replant disease（ARD） causes the inhibition of root system development, stunts tree growth and so on. To further investigate the effects of ARD on apple fruits, a 25-year-old apple orchard was remediated to est...Apple replant disease（ARD） causes the inhibition of root system development, stunts tree growth and so on. To further investigate the effects of ARD on apple fruits, a 25-year-old apple orchard was remediated to establish a replant orchard between November 2008 and March 2009. A rotational cropping orchard was established on an adjacent wheat field. The cultivar and rootstock-scion combination used in the newly established orchards was Royal Gala/M26/Malus hupehensis Rehd. Ripe fruits were collected in mid-August 2011 and mid-August 2012, meanwhile, the following indices were measured： yield per plant; fruit weight; the fruit shape index; the contents of anthocyanin, carotenoid and chlorophyll; the soluble sugar content in the flesh; titratable acid; the sugar-acid ratio; firmness; and aroma components; apple plant ground diameter, plant height increment and the total length of the current-year shoots. The results showed that compared to rotational cropping, continuous cropping yielded statistically significant reductions in fruit weight and yield per plant of 39.8 and 76.5%, respectively. However, there were no changes in the fruit shape index. The anthocyanin and carotenoid contents decreased by 81.7 and 37.7%, respectively, while the chlorophyll content increased by 251.0%. All of these differences in content were statistically significant. The soluble sugar levels and sugar-acid ratio decreased by 25.4 and 60.9%, respectively, but the titratable acid levels and fruit firmness increased by 90.9 and 42.8%, respectively. Ten of the most important esters contributing to the apple aroma were analyzed, and the following changes were observed： hexyl acetate, butyl acetate, hexyl butyrate, acetate-2-methyl butyl, 2-methyl-hexyl butyrate, amyl acetate, butyl butyrate, 2-methyl-butyl butyrate, hexyl propionate and hexyl hexanoate decreased by 25.5, 78.4, 89.1, 55.5, 79.5, 77.2, 86.8, 69.9, 61.2, and 68.1%, respectively. The contents of three other aroma components,（E）-2-hexenal, hexanal and 1-hexanol, significantly increased. Eight characteristic aroma components were found in the rotational cropping fruits： hexyl acetate, butyl acetate, acetate-2-methyl butyl, 2-methyl-hexyl butyrate, amyl acetate, 2-methyl- butyl butyrate, hexyl acetate and hexyl propionate. There were four characteristic ester components（hexyl acetate, butyl acetate, acetate-2-methyl butyl, 2-methyl-hexyl butyrate） and two characteristic aldehyde aroma components（（E）-2-hexenal and hexanal） in the continuous cropping fruits. Compared with the rotational cropping fruits, four characteristic ester components were declined and two characteristic aldehyde aroma components were increased. Compared with the control, replanted apple plant ground diameter, plant height increment and the total length of the current-year shoots were reduced by 27.6, 40.6 and 72.2%, respectively.展开更多
The herbaceous peony(Paeonia lactiflora Pall.)has high ornamental value.Replanting problems occur when seedlings are replanted into previous holes.We studied the root system and soil environment of the'Dongjingnvl...The herbaceous peony(Paeonia lactiflora Pall.)has high ornamental value.Replanting problems occur when seedlings are replanted into previous holes.We studied the root system and soil environment of the'Dongjingnvlang'variety under a continuous planting regime of one,four,and seven years,and a replanting regime of one and four years.Under the condition of continuous planting,with the increase of number of years,pH,ammonium nitrogen,and nitrate nitrogen decreased in the rhizosphere and non-rhizosphere soils,whereas organic matter,available phosphorus and potassium,enzyme activities,and the number of bacteria,fungi,and actinomycetes increased.Under the condition of replanting,with the increase of number of years,fungi and actinomycetes in both soils increased,while pH,organic matter,nutrients,enzyme activities,and bacterial number decreased.pH,organic matter,nutrient content,enzyme activity and the number of bacterial were lower in soil replanted for four years,whereas the abundance of fungi and actinomycetes was higher,altering the soil from“bacterial high-fertility”to“fungal low-fertility”with increasing years of replanting.The activity of antioxidant enzymes and MDA content in roots of peony in replanting were higher than those in continuous planting,while the content of osmotic regulatory substances in replanting was lower than that in continuous planting.The results showed that there were no obvious adverse factors in soil during seven years of continuous planting,and herbaceous peony could maintain normal growth and development.However,soils after four years of replanting were not suitable for herbaceous peony growth.Benzoic acid increased with years of replanting,which potentially caused replanting problems.This study provides a theoretical basis for understanding the mechanism of replanting problems in the herbaceous peony.展开更多
This paper examines the significance of innovative replanting strategies in maximizing oil palm yield while ensuring sustainable productivity.Through a comprehensive review of literature and analysis of current practi...This paper examines the significance of innovative replanting strategies in maximizing oil palm yield while ensuring sustainable productivity.Through a comprehensive review of literature and analysis of current practices,the major findings of this research highlighted the importance of advanced breeding and clonal selection in developing high-yielding and disease-resistant oil palm varieties.Precision agriculture technologies,including IoT devices,drones,and sensors,were identified as critical tools for data-driven decision making,optimizing resource efficiency,and reducing environmental impact.Sustainable land use planning and agroforestry integration emerged as key strategies to balance productivity with environmental conservation.The broader impacts of this work extend to other agricultural sectors and land use planning,offering valuable insights for policymakers and stakeholders to promote responsible and resilient agricultural practices.By embracing innovative replanting strategies,the oil palm industry can contribute to a more sustainable and prosperous future,balancing economic growth with environmental stewardship.Continued research and collaboration are essential to achieve these goals and foster a harmonious coexistence between productivity and sustainability,integrating precision agriculture technologies for resource optimization and reduced environmental impact,promoting sustainable land use planning and agroforestry integration to enhance biodiversity and ecosystem services.Strengthening collaborations between governments,industry players,and research institutions for innovation and knowledge exchange is essential.展开更多
The cultivation of Panax plants is hindered by replanting problems, which may be caused by plantdriven changes in the soil microbial community. Inoculation with microbial antagonists may efficiently alleviate replanti...The cultivation of Panax plants is hindered by replanting problems, which may be caused by plantdriven changes in the soil microbial community. Inoculation with microbial antagonists may efficiently alleviate replanting issues. Through high-throughput sequencing, this study revealed that bacterial diversity decreased,whereas fungal diversity increased, in the rhizosphere soils of adult ginseng plants at the root growth stage under different ages. Few microbial community, such as Luteolibacter, Cytophagaceae, Luteibacter, Sphingomonas,Sphingomonadaceae, and Zygomycota, were observed; the relative abundance of microorganisms, namely,Brevundimonas, Enterobacteriaceae, Pandoraea, Cantharellales, Dendryphion, Fusarium, and Chytridiomycota,increased in the soils of adult ginseng plants compared with those in the soils of 2-year-old seedlings. Bacillus subtilis 50-1, a microbial antagonist against the pathogenic Fusarium oxysporum, was isolated through a dual culture technique. These bacteria acted with a biocontrol efficacy of 67.8%. The ginseng death rate and Fusarium abundance decreased by 63.3% and 46.1%, respectively, after inoculation with B. subtilis 50-1. Data revealed that microecological degradation could result from ginseng-driven changes in rhizospheric microbial communities;these changes are associated with the different ages and developmental stages of ginseng plants. Biocontrol using microbial antagonists alleviated the replanting problem.展开更多
BACKGROUND Thumb replantation following complete traumatic avulsion requires complex techniques to restore function,especially in cases of avulsion at the level of the metacarpophalangeal joint(MCP I)and avulsion of t...BACKGROUND Thumb replantation following complete traumatic avulsion requires complex techniques to restore function,especially in cases of avulsion at the level of the metacarpophalangeal joint(MCP I)and avulsion of the flexor pollicis longus(FPL)at the musculotendinous junction.Possible treatments include direct tendon suture or tendon transfer,most commonly from the ring finger.To optimize function and avoid donor finger complications,we performed thumb replantation with flexion restoration using brachioradialis(BR)tendon transfer with palmaris longus(PL)tendon graft.CASE SUMMARY A 20-year-old left-handed male was admitted for a complete traumatic left thumb amputation following an accident while sliding from the top of a handrail.The patient presented with skin and bone avulsion at the MCP I,avulsion of the FPL tendon at the musculotendinous junction(zone 5),avulsion of the extensor pollicis longus tendon(zone T3),and avulsion of the thumb’s collateral arteries and nerves.The patient was treated with two stage thumb repair.The first intervention consisted of thumb replantation with MCP I arthrodesis,resection of avulsed FPL tendon and implantation of a silicone tendon prosthesis.The second intervention consisted of PL tendon graft and BR tendon transfer.Follow-up at 10 months showed good outcomes with active interphalangeal flexion of 70°,grip strength of 45 kg,key pinch strength of 15 kg and two-point discrimination threshold of 4 mm.CONCLUSION Flexion restoration after complete thumb amputation with FPL avulsion at the musculotendinous junction can be achieved using BR tendon transfer with PL tendon graft.展开更多
[Objectives]To explore the effects of motivational interviewing intervention based on the transtheoretical model(TTM)on psychological resilience and self-management in patients undergoing finger reimplantation after a...[Objectives]To explore the effects of motivational interviewing intervention based on the transtheoretical model(TTM)on psychological resilience and self-management in patients undergoing finger reimplantation after amputation.[Methods]The patients with finger replantation due to fractures admitted from October 2024 to June 2025 were divided into either the control group or the observation group according to the random number table method,with 40 cases in each group.The control group received conventional perioperative care,while the observation group underwent motivational interviewing based on TTM framework on the basis of the control group.The psychological resilience and self-management levels of the two patient groups were then compared following their respective care interventions.[Results]The psychological resilience and self-management scores of the patients were significantly higher than those of the control group after the intervention,with the difference being statistically significant(P<0.05).[Conclusions]Motivational interviewing based on the TTM can effectively improve the psychological resilience and self-management in patients undergoing severed finger reimplantation,while effectively reducing the occurrence of vascular crisis.展开更多
The repair of the periodontal membrane is essential for the successful management of periodontal disease and dental trauma.Emdogain®(EMD)is widely used in periodontal therapy due to its ability to promote repair....The repair of the periodontal membrane is essential for the successful management of periodontal disease and dental trauma.Emdogain®(EMD)is widely used in periodontal therapy due to its ability to promote repair.Despite substantial research,the cellular and molecular mechanisms underlying EMD’s effects,particularly at the single-cell resolution,remain incompletely understood.This study established a delayed tooth replantation model in rats to investigate these aspects.Tooth loss rate and degree of loosening were evaluated at 4 and 8 weeks.Micro-CT,HE staining,TRAP staining,and immunofluorescence staining were evaluated to assess EMD’s efficacy.Single-cell sequencing analyses generated single-cell maps that explored enrichment pathways,cell communication,and potential repair mechanisms.Findings indicated that EMD could reduce the rate of tooth loss,promote periodontal membrane repair,and reduce root and bone resorption.Single-cell analysis revealed that EMD promotes the importance of Vtn+fibroblasts,enhancing matrix and tissue regeneration functions.Additionally,EMD stimulated osteogenic pathways,reduced osteoclastic activity,and promoted angiogenesis-related pathways,particularly bone-related H-type vessel expression in endothelial cells.Gene modules associated with angiogenesis,osteogenesis,and odontoblast differentiation were identified,suggesting EMD might facilitate osteogenesis and odontoblast differentiation by upregulating endothelium-related genes.Immune cell analysis indicated that EMD did not elicit a significant immune response.Cell communication analysis suggested that EMD fostered pro-regenerative networks driven by interactions between mesenchymal stem cells,fibroblasts,and endothelial cells.In conclusion,EMD proves to be an effective root surface therapy agent that supports the restoration of delayed replantation teeth.展开更多
Intentional tooth replantation(ITR)is an advanced treatment modality and the procedure of last resort for preserving teeth with inaccessible endodontic or resorptive lesions.ITR is defined as the deliberate extraction...Intentional tooth replantation(ITR)is an advanced treatment modality and the procedure of last resort for preserving teeth with inaccessible endodontic or resorptive lesions.ITR is defined as the deliberate extraction of a tooth;evaluation of the root surface,endodontic manipulation,and repair;and placement of the tooth back into its original socket.Case reports,case series,cohort studies,and randomized controlled trials have demonstrated the efficacy of ITR in the retention of natural teeth that are untreatable or difficult to manage with root canal treatment or endodontic microsurgery.However,variations in clinical protocols for ITR exist due to the empirical nature of the original protocols and rapid advancements in the field of oral biology and dental materials.This heterogeneity in protocols may cause confusion among dental practitioners;therefore,guidelines and considerations for ITR should be explicated.This expert consensus discusses the biological foundation of ITR,the available clinical protocols and current status of ITR in treating teeth with refractory apical periodontitis or anatomical aberration,and the main complications of this treatment,aiming to refine the clinical management of ITR in accordance with the progress of basic research and clinical studies;the findings suggest that ITR may become a more consistent evidence-based option in dental treatment.展开更多
The cultivation of apples in replanted orchards is essential given limitations in land resources.However,the presence of Fusarium and phenolic acids in the replanted soil harms the soil environment,which impedes the s...The cultivation of apples in replanted orchards is essential given limitations in land resources.However,the presence of Fusarium and phenolic acids in the replanted soil harms the soil environment,which impedes the sustainable development of the apple industry.In this study,earthworm was used as the fermentation precursor protein to optimize the fermentation conditions,and the inhibition mechanism of the fermentation product on Fusarium and its potential to repair the apple replant soil environment were explored.Laboratory experiments showed that the optimum initial pH,temperature and time of earthworm fermentation were 7,37℃ and 10 d,respectively.The inhibition rates of earthworm fermentation products against F.oxysporum,F.solani,F.proliferatum,and F.moniliforme were 79.8%,75.1%,78.7%and 79.2%,respectively.The inhibition rates of spore germination on F.oxysporum,F.solani,F.proliferatum,and F.moniliforme were 83.8%,87.3%,83.2%and84.8%,respectively.In the field,use 300 mL of earthworm fermentation products for each planting pits before planting.The experimental results showed that,compared with the control,the content of soil pathogenic Fusarium and phenolic acid in Wantou(W3)were decreased by75.1%and 59.8%,respectively,after treatment with earthworm fermentation products in 2019.Soil urease,phosphatase,sucrase and catalase activities increased by 383.2%,78.2%,130.3%and 43.5%,respectively.The fruit weight,anthocyanin content,soluble sugar,sugar-acid ratio,total ester ratio,total ester concentration and yield increased by 80.7%,60.6%,25.6%,50.3%,19.7%,262.4%and 193.5%,respectively,while titratable acid content decreased by 16.9%.In conclusion,earthworm fermentation products can be used as a sustainable amendment to control apple replant disease.展开更多
Apple replant disease is a complex soil syndrome that occurs when the same fields are repeatedly utilized for apple orchard cultivation.It can be caused by various pathogens,and Fusarium solani is the main pathogen.Fu...Apple replant disease is a complex soil syndrome that occurs when the same fields are repeatedly utilized for apple orchard cultivation.It can be caused by various pathogens,and Fusarium solani is the main pathogen.Fusarium solani disrupts the structure and function of the orchard soil ecosystem and inhibits the growth and development of apple trees,significantly impacting the quality and yield of apples.In this study,we conducted a transcriptome comparison between uninoculated apple saplings and those inoculated with F:solani.The differentially expressed genes were mainly enriched in processes such as response to symbiotic fungus.Plant defensins are antimicrobial peptides,but their roles during F.solani infection remain unclear.We performed a genome-wide identification of apple defensin genes and identified 25 genes with the conserved motif of eight cysteine residues.In wildtype apple rootstock inoculated with F.solani,the root surface cells experienced severe damage,and showed significant differences in the total root length,total root projection area,root tips,root forks,and total root surface area compared to the control group.qRT-PCR analysis revealed that MdDEF3 and MdDEF25 were triggered in response to F.solani infection in apples.Subcellular localization showed specific expression of the MdDEF3-YFP and MdDEF25-YFP proteins on the cell membrane.Overexpressing theMdDEF25-YFP fusiongene enhanced resistance against F.solani in apple,providing a new strategy for the future prevention and biological control of apple replantdisease.展开更多
Autotoxicity stress is the principal factor in peach replant problem.Benzoic acid(BA)is known as a critical autotoxin in replant problem,and causes an obvious inhibitory effect on peach growth.Small heat shock protein...Autotoxicity stress is the principal factor in peach replant problem.Benzoic acid(BA)is known as a critical autotoxin in replant problem,and causes an obvious inhibitory effect on peach growth.Small heat shock proteins(sHSPs)have been reported to play pivotal roles in a variety of physiological and biological processes in various plants.Nevertheless,little is known about the functions and the underlying physiological mechanisms of sHSPs under autotoxicity stress.Here,we identified PpHSP20-26 of peach(Prunus persica)and deciphered its role in BA stress response.PpHSP20-26 was significantly induced by BA treatment.Overexpression of PpHSP20-26 elevated BA tolerance in Arabidopsis and peach plants,whereas down-regulation of PpHSP20-26 in peach through virus-induced gene silencing enhanced BA sensitivity.Compared to the control,the PpHSP20-26-overexpressing plants exhibited lower contents of reactive oxygen species(ROS)and higher activities of antioxidant enzymes.Furthermore,PpHSP20-26 regulated the transcripts of stress-responsive genes including CAT,SOD,APX,GPX,DHAR,and ABC transporters in overexpressing Arabidopsis and silenced peach plants.Taken together,these data suggest that PpHSP20-26 plays a positive role in peach response to BA stress by,at least partly,regulating ROS metabolism and stress-responsive gene expression.Our findings will be of great importance for further understanding the roles of sHSPs genes in autotoxicity stress,and assist crop breeding in mitigating replant problem.展开更多
Apple replant disease(ARD)negatively affects plant growth and reduces yields in replanted orchards.In this study,biochar was applied to apple replant soil with Fusarium oxysporum.Our aim was to investigate whether bio...Apple replant disease(ARD)negatively affects plant growth and reduces yields in replanted orchards.In this study,biochar was applied to apple replant soil with Fusarium oxysporum.Our aim was to investigate whether biochar could promote plant growth and alleviate apple replant disease by reducing the growth of harmful soil microorganisms,changing soil microbial community structure and improving the soil environment.This experiment included five treatments:apple replant soil(CK),methyl bromide fumigation apple replant soil(FM),replant soil with biochar addition(2%),replant soil with F.oxysporum spore solution(8×10^(7)spores·mL^(-1)),and replant soil with biochar and F.oxysporum spore solution addition.Seedling biomass,the activity of antioxidant enzymes in the leaves and roots,and soil environmental variables were measured.Microbial community composition and community structure were analyzed using 16SrDNA and ITS2 gene sequencing.Biochar significantly reduced the abundance of F.oxysporum and increased soil microbial diversity and richness.Biochar also increased the soil enzyme activities(urease,invertase,neutral phosphatase,and catalase),the biomass(plant height,fresh weight,dry weight)and the activity of antioxidant enzymes(superoxide dismutase,peroxidase,and catalase).The root indexes of apple seedlings was also increased in replant soil by biochar.In sum,biochar promoted the growth of plants,improved the replant soil environment,and alleviated apple replant disease.展开更多
Apple replant disease(ARD) is primarily caused by biotic factors that seriously inhibits the development of apple industry. Therefore, the use of biological control measures to inhibit the main pathogens(such as Fusar...Apple replant disease(ARD) is primarily caused by biotic factors that seriously inhibits the development of apple industry. Therefore, the use of biological control measures to inhibit the main pathogens(such as Fusarium spp.) that cause ARD is of great significance to the sustainable development of the apple industry. Trichoderma virens 6PS-2, which exhibited antagonism toward a variety of pathogens, was screened from the rhizosphere soils of healthy apple trees(Malus robusta) in different replanted orchards in the Yantai and Zibo Cities, Shandong Province, China. Its fermentation extract inhibited the growth of pathogenic Fusarium proliferatum f. sp. Malus domestica MR5, which was proportional to the concentration. These substances also increased the hairy root volume and growth of Arabidopsis thaliana lateral roots. The phenotype of Malus hupehensis seedlings and microbial community structure in rhizosphere soils in greenhouse experiment using Highthroughput sequencing were analyzed, and the field experiment with grafted apple trees were used for further verification. Compared with the application of potato dextrose broth(PDB) medium, application of 6PS-2 spore suspension directly to replanted soils could improve the growth of M. hupehensis seedlings as well as the elongation of grafted apple trees. Concomitant decreases in the gene copy number of Fusarium and increases in the culturable bacteria/fungi were also observed in the greenhouse and field experiments. The abundance of Trichoderma,Bacillus, and Streptomyces increased significantly, but that of Fusarium, Pseudarthrobacter, and Humicola decreased. The content of esters, phenols,furans, and amino acids in root exudates of M. hupehensis seedlings increased, which significantly inhibited the multiplication of Fusarium, but was positively correlated with Bacillus and Trichoderma. In summary, T. virens 6PS-2 not only directly inhibits the activity of pathogenic Fusarium but also secrets secondary metabolites with antifungal and growth-promoting potential. In addition, 6PS-2 spore suspension can also promote the growth of plants to a certain extent, and change the soil microbial community structure of rhizosphere soils. It is believed that T. virens 6PS-2 has the potential for the alleviation of apple replant disease(ARD) in China.展开更多
Apple replant disease(ARD)has led to severe yield and quality reduction in the apple industry.Fusarium solani(F.solani)has been identified as one of the main microbial pathogens responsible for ARD.Auxin(indole-3-acet...Apple replant disease(ARD)has led to severe yield and quality reduction in the apple industry.Fusarium solani(F.solani)has been identified as one of the main microbial pathogens responsible for ARD.Auxin(indole-3-acetic acid,IAA),an endogenous hormone in plants,is involved in almost all plant growth and development processes and plays a role in plant immunity against pathogens.Gretchen Hagen3(GH3)is one of the early/primary auxin response genes.The aim of this study was to evaluate the function of MdGH3-2 and MdGH3-12 in the defense response of F.solani by treating MdGH3-2/12 RNAi plants with F.solani.The results show that under F.solani infection,RNAi of MdGH3-2/12 inhibited plant biomass accumulation and exacerbated root damage.After inoculation with F.solani,MdGH3-2/12 RNAi inhibited the biosynthesis of acid-amido synthetase.This led to the inhibition of free IAA combining with amino acids,resulting in excessive free IAA accumulation.This excessive free IAA altered plant tissue structure,accelerated fungal hyphal invasion,reduced the activity of antioxidant enzymes(SOD,POD and CAT),increased the reactive oxygen species(ROS)level,and reduced total chlorophyll content and photosynthetic ability,while regulating the expression of PR-related genes including PR1,PR4,PR5 and PR8.It also changed the contents of plant hormones and amino acids,and ultimately reduced the resistance to F.solani.In conclusion,these results demonstrate that MdGH3-2 and MdGH3-12 play an important role in apple tolerance to F.solani and ARD.展开更多
Objective:To investigate the clinical effect of perioperative intensive nursing on patients who underwent severed finger reimplantation and its influence on sleep quality.Methods:62 patients who underwent severed fing...Objective:To investigate the clinical effect of perioperative intensive nursing on patients who underwent severed finger reimplantation and its influence on sleep quality.Methods:62 patients who underwent severed finger reimplantation between January 2023 and December 2023 were included.They were divided into two groups:the observation group(n=31)and the control group(n=31).The control group received basic nursing interventions,while the observation group received intensive nursing care.The comparison parameters included visual analog pain score(VAS),incidence of vascular crises,length of hospitalization,Pittsburgh Sleep Quality Index(PSQI),Hamilton Depression Score(HAM-D),Hamilton Anxiety Score(HAM-A),and patient satisfaction.Results:Postoperative VAS score,incidence of vascular crisis,hospitalization time,PSQI score,and HAM-A and HAM-D scores of the observation group were lower than those of the control group(P<0.05).Meanwhile,the patient satisfaction of the observation group was higher than that of the control group(P<0.05).Conclusion:Perioperative intensive nursing care for patients undergoing severed finger reimplantation demonstrates significant benefits.It reduces postoperative pain,lowers the incidence of vascular crises,shortens hospitalization durations,enhances sleep quality,alleviates negative emotions,and improves nursing satisfaction.These findings underscore the importance and applicability of such care practices.展开更多
This study was conducted to generate information required to guide plantation management in relation to replanting on soils derived from different parent materials. Six oil palm estates in coastal lowlands of southwes...This study was conducted to generate information required to guide plantation management in relation to replanting on soils derived from different parent materials. Six oil palm estates in coastal lowlands of southwest Cameroon were considered. Oil palm yield data (in t⋅ha−1 of fresh fruit bunch, FFB) and corresponding age of palms (in years after planting, YAP) were obtained for the various estates. In all the estates, average yields were ⋅FFB⋅ha−1 and highly variable. Plantation age, solely, explained between 20% - 58% of the variation in yield. The highest average yields (11.5 t⋅FFB⋅ha−1) were obtained in plantations aged between 9 and 18 YAP and the lowest (4.66 t⋅FFB⋅ha−1) were obtained in old plantations (>23 YAP). Plantations located on volcanic parent materials generally had higher yields compared to those established on sedimentary parent materials. In order to intensify production and increase yields while conserving the environment, one important measure to consider is the replacement of aged palms, and the recommended optimal replanting age in coastal plains of southwest Cameroon should be at most 23 YAP. Estimated mean yields, if aged palms are replanted on time, can increase by 43% - 65%. Additionally, site-specific nutrient management options should be considered in plantation intensification programs.展开更多
Objective: To evaluate the value of inactivated bone replantation with preservation of the epiphysis following the effective chemotherapy in avoiding postoperative discrepancy of the affected limb in children with ost...Objective: To evaluate the value of inactivated bone replantation with preservation of the epiphysis following the effective chemotherapy in avoiding postoperative discrepancy of the affected limb in children with osteosarcoma. Methods: Two children (aged 5 and 10 years, 1 male and 1 female) with osteosarcoma underwent inactivated bone replantation with preserving epiphysis following chemotherapy (MMIA protocol, including high-dose methotrexate, adriamycin and ifosfamide). After two cycles of preop-erative chemotherapy, pain vanished, the local mass shrank and there was no pain on pressing the affected parts. Sera AKP and LDH were reduced to normal levels; marked shrinkage and sclerotic changes and good margin of lesions were seen on plain radiographs and MR images. Two courses of the same protocol as preoperative chemotherapy were administered postoperatively. Results: Postoperative histological examination of the specimens demonstrated absence of vital tumor cells. Incisions healed well and no complications occurred. The replanted inactivated bone healed with host at 6 months after operation. In the two patients, no evidence was seen of metastasis and recurrence and discrepancy of the affected limbs in postoperative 36 and 48 months. Functions of the affected limbs were satisfactory. Conclusion: Inactivated bone replantation with preserving epiphysis was a viable option for osteosarcoma in children. The long-term outcomes remain to be further proven.展开更多
Rangelands contribute to human well-being worldwide.However,its fragile ecosystems are threatened due to inappropriate management that has been leading to its degradation in African rangelands in general and in Ethiop...Rangelands contribute to human well-being worldwide.However,its fragile ecosystems are threatened due to inappropriate management that has been leading to its degradation in African rangelands in general and in Ethiopian rangelands in particular.Rangeland degradation is attributable to both natural and anthropogenic causes.Restoring degraded areas by replanting using native species is one of the most promising sustainable rangeland management tools to fight the degradation in the rangelands and enhance resilience in the face of environmental shocks.Restoration improves vegetation cover and biomass yield and enhances other ecosystem services.Native drought-tolerant species have produced promising rehabilitation outcomes and have been recommended for the restoration of degraded rangeland areas.Replanting using native species remains a viable sustainable management option to enhance resilience in the face of environmental shocks.Therefore,to maintain the sustainability and resilient rangeland ecosystems,comprehensive approaches and strategies suitable for rangelands need to be revitalized,developed,strengthened and promoted.展开更多
Soil fungi play a very important role in the soil ecological environment. In agricultural production, long-term monoculture and continuous cropping lead to changes in fungal community diversity. However, the effects o...Soil fungi play a very important role in the soil ecological environment. In agricultural production, long-term monoculture and continuous cropping lead to changes in fungal community diversity. However, the effects of long-term monoculture and continuous cropping on strawberry plant health and fungal community diversity have not been elucidated. In this study, using high-throughput sequencing(HTS), we compared the fungal community and diversity of strawberry rhizosphere soil after various durations of continuous cropping(0, 2, 4, 6, 8, 10 and 12 years). The results showed that soil fungal diversity increased with consecutive cropping years. Specifically, the soil-borne disease pathogens Fusarium and Guehomyces were significantly increased after strawberry continuous cropping, and the abundance of nematicidal(Arthrobotrys) fungi decreased from the fourth year of continuous cropping. The results of correlation analysis suggest that these three genera might be key fungi that contribute to the changes in soil properties that occur during continuous cropping. In addition, physicochemical property analysis showed that the soil nutrient content began to decline after the fourth year of continuous cropping. Spearman's correlation analysis showed that soil pH, available potassium(AK) and ammonium nitrogen(NH_4^+-N) were the most important edaphic factors leading to contrasting beneficial and pathogenic associations across consecutive strawberry cropping systems.展开更多
A two-year field experiment was carried out in order to study the occurrence degree and mechanism of apple replant disease(ARD)in the apple orchards with different soil textures.So we can adopt appropriate controlmeas...A two-year field experiment was carried out in order to study the occurrence degree and mechanism of apple replant disease(ARD)in the apple orchards with different soil textures.So we can adopt appropriate controlmeasures according to the severity of ARD.Healthy two-year-old seedlings with consistent growth were selected,of which the root stock was T337 and the scion was Yanfu 3.There were significant differences in biomass between methyl bromide fumigation and replanted treatments,and the difference was the largest in clay loam,followed by sandy loam,and loam,which verified ARD in clay loam was most serious,followed by sandy loam and loam.Based on high-throughput sequencing of fungi in soil samples,fungal richness and diversity were the highest in clay loam,followed by sandy loam,and loam.The relative abundance of Fusarium in SX,SL,FX,FL,WX and WL was 7.33%,19.32%,2.70%,4.24%,10.71%and 23.87%,respectively.Based on Real-time quantitative analysis,there were significant differences in the number of Fusarium oxysporum and Fusarium solani between methyl bromide fumigation and replanted treatments,i.e.,clay loam>sandy loam>loam.Fusarium was the main pathogen causing ARD.This shows that ARD is the most serious under replanted clay loam condition.High-throughput sequencing technology was used to prove the difference in Fusarium was one of the important reasons for ARD under different soil textures.This technology provides a new idea for the prevention and control of ARD.展开更多
基金supported by the Earmarked Fund for National Modern Agro-industry Technology Research System (CARS-28), Chinathe Key Innovation Project for Agricultural Application Technology of Shandong Province, ChinaYangtze River Scholar and Innovative Team Development Plan of the Ministry of Education, China (IRT1155)
文摘Apple replant disease（ARD） causes the inhibition of root system development, stunts tree growth and so on. To further investigate the effects of ARD on apple fruits, a 25-year-old apple orchard was remediated to establish a replant orchard between November 2008 and March 2009. A rotational cropping orchard was established on an adjacent wheat field. The cultivar and rootstock-scion combination used in the newly established orchards was Royal Gala/M26/Malus hupehensis Rehd. Ripe fruits were collected in mid-August 2011 and mid-August 2012, meanwhile, the following indices were measured： yield per plant; fruit weight; the fruit shape index; the contents of anthocyanin, carotenoid and chlorophyll; the soluble sugar content in the flesh; titratable acid; the sugar-acid ratio; firmness; and aroma components; apple plant ground diameter, plant height increment and the total length of the current-year shoots. The results showed that compared to rotational cropping, continuous cropping yielded statistically significant reductions in fruit weight and yield per plant of 39.8 and 76.5%, respectively. However, there were no changes in the fruit shape index. The anthocyanin and carotenoid contents decreased by 81.7 and 37.7%, respectively, while the chlorophyll content increased by 251.0%. All of these differences in content were statistically significant. The soluble sugar levels and sugar-acid ratio decreased by 25.4 and 60.9%, respectively, but the titratable acid levels and fruit firmness increased by 90.9 and 42.8%, respectively. Ten of the most important esters contributing to the apple aroma were analyzed, and the following changes were observed： hexyl acetate, butyl acetate, hexyl butyrate, acetate-2-methyl butyl, 2-methyl-hexyl butyrate, amyl acetate, butyl butyrate, 2-methyl-butyl butyrate, hexyl propionate and hexyl hexanoate decreased by 25.5, 78.4, 89.1, 55.5, 79.5, 77.2, 86.8, 69.9, 61.2, and 68.1%, respectively. The contents of three other aroma components,（E）-2-hexenal, hexanal and 1-hexanol, significantly increased. Eight characteristic aroma components were found in the rotational cropping fruits： hexyl acetate, butyl acetate, acetate-2-methyl butyl, 2-methyl-hexyl butyrate, amyl acetate, 2-methyl- butyl butyrate, hexyl acetate and hexyl propionate. There were four characteristic ester components（hexyl acetate, butyl acetate, acetate-2-methyl butyl, 2-methyl-hexyl butyrate） and two characteristic aldehyde aroma components（（E）-2-hexenal and hexanal） in the continuous cropping fruits. Compared with the rotational cropping fruits, four characteristic ester components were declined and two characteristic aldehyde aroma components were increased. Compared with the control, replanted apple plant ground diameter, plant height increment and the total length of the current-year shoots were reduced by 27.6, 40.6 and 72.2%, respectively.
基金supported by grants from the National Natural Science Foundation of China(Grant No.31670663).
文摘The herbaceous peony(Paeonia lactiflora Pall.)has high ornamental value.Replanting problems occur when seedlings are replanted into previous holes.We studied the root system and soil environment of the'Dongjingnvlang'variety under a continuous planting regime of one,four,and seven years,and a replanting regime of one and four years.Under the condition of continuous planting,with the increase of number of years,pH,ammonium nitrogen,and nitrate nitrogen decreased in the rhizosphere and non-rhizosphere soils,whereas organic matter,available phosphorus and potassium,enzyme activities,and the number of bacteria,fungi,and actinomycetes increased.Under the condition of replanting,with the increase of number of years,fungi and actinomycetes in both soils increased,while pH,organic matter,nutrients,enzyme activities,and bacterial number decreased.pH,organic matter,nutrient content,enzyme activity and the number of bacterial were lower in soil replanted for four years,whereas the abundance of fungi and actinomycetes was higher,altering the soil from“bacterial high-fertility”to“fungal low-fertility”with increasing years of replanting.The activity of antioxidant enzymes and MDA content in roots of peony in replanting were higher than those in continuous planting,while the content of osmotic regulatory substances in replanting was lower than that in continuous planting.The results showed that there were no obvious adverse factors in soil during seven years of continuous planting,and herbaceous peony could maintain normal growth and development.However,soils after four years of replanting were not suitable for herbaceous peony growth.Benzoic acid increased with years of replanting,which potentially caused replanting problems.This study provides a theoretical basis for understanding the mechanism of replanting problems in the herbaceous peony.
基金support from the Universiti Putra Malaysia Fundamental Research Grant Scheme(FRGS 1/2020/WAB04/Vote no 5540305)D’Khairan Farm Sdn Bhd(Vote no 6300349).
文摘This paper examines the significance of innovative replanting strategies in maximizing oil palm yield while ensuring sustainable productivity.Through a comprehensive review of literature and analysis of current practices,the major findings of this research highlighted the importance of advanced breeding and clonal selection in developing high-yielding and disease-resistant oil palm varieties.Precision agriculture technologies,including IoT devices,drones,and sensors,were identified as critical tools for data-driven decision making,optimizing resource efficiency,and reducing environmental impact.Sustainable land use planning and agroforestry integration emerged as key strategies to balance productivity with environmental conservation.The broader impacts of this work extend to other agricultural sectors and land use planning,offering valuable insights for policymakers and stakeholders to promote responsible and resilient agricultural practices.By embracing innovative replanting strategies,the oil palm industry can contribute to a more sustainable and prosperous future,balancing economic growth with environmental stewardship.Continued research and collaboration are essential to achieve these goals and foster a harmonious coexistence between productivity and sustainability,integrating precision agriculture technologies for resource optimization and reduced environmental impact,promoting sustainable land use planning and agroforestry integration to enhance biodiversity and ecosystem services.Strengthening collaborations between governments,industry players,and research institutions for innovation and knowledge exchange is essential.
基金supported by grants from the National Science Foundation of China(81603238)
文摘The cultivation of Panax plants is hindered by replanting problems, which may be caused by plantdriven changes in the soil microbial community. Inoculation with microbial antagonists may efficiently alleviate replanting issues. Through high-throughput sequencing, this study revealed that bacterial diversity decreased,whereas fungal diversity increased, in the rhizosphere soils of adult ginseng plants at the root growth stage under different ages. Few microbial community, such as Luteolibacter, Cytophagaceae, Luteibacter, Sphingomonas,Sphingomonadaceae, and Zygomycota, were observed; the relative abundance of microorganisms, namely,Brevundimonas, Enterobacteriaceae, Pandoraea, Cantharellales, Dendryphion, Fusarium, and Chytridiomycota,increased in the soils of adult ginseng plants compared with those in the soils of 2-year-old seedlings. Bacillus subtilis 50-1, a microbial antagonist against the pathogenic Fusarium oxysporum, was isolated through a dual culture technique. These bacteria acted with a biocontrol efficacy of 67.8%. The ginseng death rate and Fusarium abundance decreased by 63.3% and 46.1%, respectively, after inoculation with B. subtilis 50-1. Data revealed that microecological degradation could result from ginseng-driven changes in rhizospheric microbial communities;these changes are associated with the different ages and developmental stages of ginseng plants. Biocontrol using microbial antagonists alleviated the replanting problem.
文摘BACKGROUND Thumb replantation following complete traumatic avulsion requires complex techniques to restore function,especially in cases of avulsion at the level of the metacarpophalangeal joint(MCP I)and avulsion of the flexor pollicis longus(FPL)at the musculotendinous junction.Possible treatments include direct tendon suture or tendon transfer,most commonly from the ring finger.To optimize function and avoid donor finger complications,we performed thumb replantation with flexion restoration using brachioradialis(BR)tendon transfer with palmaris longus(PL)tendon graft.CASE SUMMARY A 20-year-old left-handed male was admitted for a complete traumatic left thumb amputation following an accident while sliding from the top of a handrail.The patient presented with skin and bone avulsion at the MCP I,avulsion of the FPL tendon at the musculotendinous junction(zone 5),avulsion of the extensor pollicis longus tendon(zone T3),and avulsion of the thumb’s collateral arteries and nerves.The patient was treated with two stage thumb repair.The first intervention consisted of thumb replantation with MCP I arthrodesis,resection of avulsed FPL tendon and implantation of a silicone tendon prosthesis.The second intervention consisted of PL tendon graft and BR tendon transfer.Follow-up at 10 months showed good outcomes with active interphalangeal flexion of 70°,grip strength of 45 kg,key pinch strength of 15 kg and two-point discrimination threshold of 4 mm.CONCLUSION Flexion restoration after complete thumb amputation with FPL avulsion at the musculotendinous junction can be achieved using BR tendon transfer with PL tendon graft.
基金Supported by 2023 Shiyan Taihe Hospital Fund Project(2023JJXM024).
文摘[Objectives]To explore the effects of motivational interviewing intervention based on the transtheoretical model(TTM)on psychological resilience and self-management in patients undergoing finger reimplantation after amputation.[Methods]The patients with finger replantation due to fractures admitted from October 2024 to June 2025 were divided into either the control group or the observation group according to the random number table method,with 40 cases in each group.The control group received conventional perioperative care,while the observation group underwent motivational interviewing based on TTM framework on the basis of the control group.The psychological resilience and self-management levels of the two patient groups were then compared following their respective care interventions.[Results]The psychological resilience and self-management scores of the patients were significantly higher than those of the control group after the intervention,with the difference being statistically significant(P<0.05).[Conclusions]Motivational interviewing based on the TTM can effectively improve the psychological resilience and self-management in patients undergoing severed finger reimplantation,while effectively reducing the occurrence of vascular crisis.
基金the National Natural Science Foundation of China(U22A20314,82470988,32070826)National Key R&D of Program of China(2022YFC2504201)+2 种基金Outstanding Youth Fund of Chongqing Natural Science Foundation(CSTB2023NSCQJQX0006)Science and Technology Research Project of Chongqing Education Commission(KJQN202200471)CQMU Program for Youth Innovation in Future Medicine(W0075).
文摘The repair of the periodontal membrane is essential for the successful management of periodontal disease and dental trauma.Emdogain®(EMD)is widely used in periodontal therapy due to its ability to promote repair.Despite substantial research,the cellular and molecular mechanisms underlying EMD’s effects,particularly at the single-cell resolution,remain incompletely understood.This study established a delayed tooth replantation model in rats to investigate these aspects.Tooth loss rate and degree of loosening were evaluated at 4 and 8 weeks.Micro-CT,HE staining,TRAP staining,and immunofluorescence staining were evaluated to assess EMD’s efficacy.Single-cell sequencing analyses generated single-cell maps that explored enrichment pathways,cell communication,and potential repair mechanisms.Findings indicated that EMD could reduce the rate of tooth loss,promote periodontal membrane repair,and reduce root and bone resorption.Single-cell analysis revealed that EMD promotes the importance of Vtn+fibroblasts,enhancing matrix and tissue regeneration functions.Additionally,EMD stimulated osteogenic pathways,reduced osteoclastic activity,and promoted angiogenesis-related pathways,particularly bone-related H-type vessel expression in endothelial cells.Gene modules associated with angiogenesis,osteogenesis,and odontoblast differentiation were identified,suggesting EMD might facilitate osteogenesis and odontoblast differentiation by upregulating endothelium-related genes.Immune cell analysis indicated that EMD did not elicit a significant immune response.Cell communication analysis suggested that EMD fostered pro-regenerative networks driven by interactions between mesenchymal stem cells,fibroblasts,and endothelial cells.In conclusion,EMD proves to be an effective root surface therapy agent that supports the restoration of delayed replantation teeth.
文摘Intentional tooth replantation(ITR)is an advanced treatment modality and the procedure of last resort for preserving teeth with inaccessible endodontic or resorptive lesions.ITR is defined as the deliberate extraction of a tooth;evaluation of the root surface,endodontic manipulation,and repair;and placement of the tooth back into its original socket.Case reports,case series,cohort studies,and randomized controlled trials have demonstrated the efficacy of ITR in the retention of natural teeth that are untreatable or difficult to manage with root canal treatment or endodontic microsurgery.However,variations in clinical protocols for ITR exist due to the empirical nature of the original protocols and rapid advancements in the field of oral biology and dental materials.This heterogeneity in protocols may cause confusion among dental practitioners;therefore,guidelines and considerations for ITR should be explicated.This expert consensus discusses the biological foundation of ITR,the available clinical protocols and current status of ITR in treating teeth with refractory apical periodontitis or anatomical aberration,and the main complications of this treatment,aiming to refine the clinical management of ITR in accordance with the progress of basic research and clinical studies;the findings suggest that ITR may become a more consistent evidence-based option in dental treatment.
基金supported by the National Natural Science Foundation of China(Grant No.31672104)the earmarked fund for China Agriculture Research System(Grant No.CARS-27)+1 种基金Taishan Scholar Funded Project(Grant No.20190923)Natural Science Foundation of Shandong Province(Grant No.ZR2020MC131)。
文摘The cultivation of apples in replanted orchards is essential given limitations in land resources.However,the presence of Fusarium and phenolic acids in the replanted soil harms the soil environment,which impedes the sustainable development of the apple industry.In this study,earthworm was used as the fermentation precursor protein to optimize the fermentation conditions,and the inhibition mechanism of the fermentation product on Fusarium and its potential to repair the apple replant soil environment were explored.Laboratory experiments showed that the optimum initial pH,temperature and time of earthworm fermentation were 7,37℃ and 10 d,respectively.The inhibition rates of earthworm fermentation products against F.oxysporum,F.solani,F.proliferatum,and F.moniliforme were 79.8%,75.1%,78.7%and 79.2%,respectively.The inhibition rates of spore germination on F.oxysporum,F.solani,F.proliferatum,and F.moniliforme were 83.8%,87.3%,83.2%and84.8%,respectively.In the field,use 300 mL of earthworm fermentation products for each planting pits before planting.The experimental results showed that,compared with the control,the content of soil pathogenic Fusarium and phenolic acid in Wantou(W3)were decreased by75.1%and 59.8%,respectively,after treatment with earthworm fermentation products in 2019.Soil urease,phosphatase,sucrase and catalase activities increased by 383.2%,78.2%,130.3%and 43.5%,respectively.The fruit weight,anthocyanin content,soluble sugar,sugar-acid ratio,total ester ratio,total ester concentration and yield increased by 80.7%,60.6%,25.6%,50.3%,19.7%,262.4%and 193.5%,respectively,while titratable acid content decreased by 16.9%.In conclusion,earthworm fermentation products can be used as a sustainable amendment to control apple replant disease.
基金supported by a project grant from the Key Research and Development and Promotion Projects of Henan Province,China(212102110113)the Special Fund for Henan Agriculture Research System,China(HARS-22-09-Z2).
文摘Apple replant disease is a complex soil syndrome that occurs when the same fields are repeatedly utilized for apple orchard cultivation.It can be caused by various pathogens,and Fusarium solani is the main pathogen.Fusarium solani disrupts the structure and function of the orchard soil ecosystem and inhibits the growth and development of apple trees,significantly impacting the quality and yield of apples.In this study,we conducted a transcriptome comparison between uninoculated apple saplings and those inoculated with F:solani.The differentially expressed genes were mainly enriched in processes such as response to symbiotic fungus.Plant defensins are antimicrobial peptides,but their roles during F.solani infection remain unclear.We performed a genome-wide identification of apple defensin genes and identified 25 genes with the conserved motif of eight cysteine residues.In wildtype apple rootstock inoculated with F.solani,the root surface cells experienced severe damage,and showed significant differences in the total root length,total root projection area,root tips,root forks,and total root surface area compared to the control group.qRT-PCR analysis revealed that MdDEF3 and MdDEF25 were triggered in response to F.solani infection in apples.Subcellular localization showed specific expression of the MdDEF3-YFP and MdDEF25-YFP proteins on the cell membrane.Overexpressing theMdDEF25-YFP fusiongene enhanced resistance against F.solani in apple,providing a new strategy for the future prevention and biological control of apple replantdisease.
基金supported by the National Natural Science Foundation of China(Grant No.32272645)China Agriculture Research System of MOF and MARA(Grant No.CARS-30)Changsha Municipal Natural Science Foundation(Grant No.kq2208135).
文摘Autotoxicity stress is the principal factor in peach replant problem.Benzoic acid(BA)is known as a critical autotoxin in replant problem,and causes an obvious inhibitory effect on peach growth.Small heat shock proteins(sHSPs)have been reported to play pivotal roles in a variety of physiological and biological processes in various plants.Nevertheless,little is known about the functions and the underlying physiological mechanisms of sHSPs under autotoxicity stress.Here,we identified PpHSP20-26 of peach(Prunus persica)and deciphered its role in BA stress response.PpHSP20-26 was significantly induced by BA treatment.Overexpression of PpHSP20-26 elevated BA tolerance in Arabidopsis and peach plants,whereas down-regulation of PpHSP20-26 in peach through virus-induced gene silencing enhanced BA sensitivity.Compared to the control,the PpHSP20-26-overexpressing plants exhibited lower contents of reactive oxygen species(ROS)and higher activities of antioxidant enzymes.Furthermore,PpHSP20-26 regulated the transcripts of stress-responsive genes including CAT,SOD,APX,GPX,DHAR,and ABC transporters in overexpressing Arabidopsis and silenced peach plants.Taken together,these data suggest that PpHSP20-26 plays a positive role in peach response to BA stress by,at least partly,regulating ROS metabolism and stress-responsive gene expression.Our findings will be of great importance for further understanding the roles of sHSPs genes in autotoxicity stress,and assist crop breeding in mitigating replant problem.
基金supported by the earmarked fund for National Natural Science Foundation of China(Grant No.31801816)National Modern Agro-industry Technology Research System(Grant No.CARS-27)Taishan scholar funded project(Grant No.TS20190923)。
文摘Apple replant disease(ARD)negatively affects plant growth and reduces yields in replanted orchards.In this study,biochar was applied to apple replant soil with Fusarium oxysporum.Our aim was to investigate whether biochar could promote plant growth and alleviate apple replant disease by reducing the growth of harmful soil microorganisms,changing soil microbial community structure and improving the soil environment.This experiment included five treatments:apple replant soil(CK),methyl bromide fumigation apple replant soil(FM),replant soil with biochar addition(2%),replant soil with F.oxysporum spore solution(8×10^(7)spores·mL^(-1)),and replant soil with biochar and F.oxysporum spore solution addition.Seedling biomass,the activity of antioxidant enzymes in the leaves and roots,and soil environmental variables were measured.Microbial community composition and community structure were analyzed using 16SrDNA and ITS2 gene sequencing.Biochar significantly reduced the abundance of F.oxysporum and increased soil microbial diversity and richness.Biochar also increased the soil enzyme activities(urease,invertase,neutral phosphatase,and catalase),the biomass(plant height,fresh weight,dry weight)and the activity of antioxidant enzymes(superoxide dismutase,peroxidase,and catalase).The root indexes of apple seedlings was also increased in replant soil by biochar.In sum,biochar promoted the growth of plants,improved the replant soil environment,and alleviated apple replant disease.
基金supported by China Agriculture Research System of MOF and MARA (Grant No.CARS-27)Shandong Agricultural Major Applied Technology Innovation Project (Grant No.SD2019ZZ008)+5 种基金Qingchuang Science and Technology Support Project of Shandong Colleges and Universities (Grant No.2019KJF020)Natural Science Foundation of Shandong Province (Grant No.ZR2020MC131)the National Key Research and Development Program of China (Grant No.2020YFD1000201)Taishan Scholars Funded Project (Grant No.ts20190923)the National Natural Science Foundation of China (Grant No.32072510)the Fruit Innovation Team in Shandong Province,China (Grant No.SDAIT-06-07)。
文摘Apple replant disease(ARD) is primarily caused by biotic factors that seriously inhibits the development of apple industry. Therefore, the use of biological control measures to inhibit the main pathogens(such as Fusarium spp.) that cause ARD is of great significance to the sustainable development of the apple industry. Trichoderma virens 6PS-2, which exhibited antagonism toward a variety of pathogens, was screened from the rhizosphere soils of healthy apple trees(Malus robusta) in different replanted orchards in the Yantai and Zibo Cities, Shandong Province, China. Its fermentation extract inhibited the growth of pathogenic Fusarium proliferatum f. sp. Malus domestica MR5, which was proportional to the concentration. These substances also increased the hairy root volume and growth of Arabidopsis thaliana lateral roots. The phenotype of Malus hupehensis seedlings and microbial community structure in rhizosphere soils in greenhouse experiment using Highthroughput sequencing were analyzed, and the field experiment with grafted apple trees were used for further verification. Compared with the application of potato dextrose broth(PDB) medium, application of 6PS-2 spore suspension directly to replanted soils could improve the growth of M. hupehensis seedlings as well as the elongation of grafted apple trees. Concomitant decreases in the gene copy number of Fusarium and increases in the culturable bacteria/fungi were also observed in the greenhouse and field experiments. The abundance of Trichoderma,Bacillus, and Streptomyces increased significantly, but that of Fusarium, Pseudarthrobacter, and Humicola decreased. The content of esters, phenols,furans, and amino acids in root exudates of M. hupehensis seedlings increased, which significantly inhibited the multiplication of Fusarium, but was positively correlated with Bacillus and Trichoderma. In summary, T. virens 6PS-2 not only directly inhibits the activity of pathogenic Fusarium but also secrets secondary metabolites with antifungal and growth-promoting potential. In addition, 6PS-2 spore suspension can also promote the growth of plants to a certain extent, and change the soil microbial community structure of rhizosphere soils. It is believed that T. virens 6PS-2 has the potential for the alleviation of apple replant disease(ARD) in China.
基金supported by the Earmarked Fund for the China Agriculture Research System(CARS-27)the Key Science and Technology Special Projects of Shaanxi Province,China(2020zdzx03-01-02).
文摘Apple replant disease(ARD)has led to severe yield and quality reduction in the apple industry.Fusarium solani(F.solani)has been identified as one of the main microbial pathogens responsible for ARD.Auxin(indole-3-acetic acid,IAA),an endogenous hormone in plants,is involved in almost all plant growth and development processes and plays a role in plant immunity against pathogens.Gretchen Hagen3(GH3)is one of the early/primary auxin response genes.The aim of this study was to evaluate the function of MdGH3-2 and MdGH3-12 in the defense response of F.solani by treating MdGH3-2/12 RNAi plants with F.solani.The results show that under F.solani infection,RNAi of MdGH3-2/12 inhibited plant biomass accumulation and exacerbated root damage.After inoculation with F.solani,MdGH3-2/12 RNAi inhibited the biosynthesis of acid-amido synthetase.This led to the inhibition of free IAA combining with amino acids,resulting in excessive free IAA accumulation.This excessive free IAA altered plant tissue structure,accelerated fungal hyphal invasion,reduced the activity of antioxidant enzymes(SOD,POD and CAT),increased the reactive oxygen species(ROS)level,and reduced total chlorophyll content and photosynthetic ability,while regulating the expression of PR-related genes including PR1,PR4,PR5 and PR8.It also changed the contents of plant hormones and amino acids,and ultimately reduced the resistance to F.solani.In conclusion,these results demonstrate that MdGH3-2 and MdGH3-12 play an important role in apple tolerance to F.solani and ARD.
文摘Objective:To investigate the clinical effect of perioperative intensive nursing on patients who underwent severed finger reimplantation and its influence on sleep quality.Methods:62 patients who underwent severed finger reimplantation between January 2023 and December 2023 were included.They were divided into two groups:the observation group(n=31)and the control group(n=31).The control group received basic nursing interventions,while the observation group received intensive nursing care.The comparison parameters included visual analog pain score(VAS),incidence of vascular crises,length of hospitalization,Pittsburgh Sleep Quality Index(PSQI),Hamilton Depression Score(HAM-D),Hamilton Anxiety Score(HAM-A),and patient satisfaction.Results:Postoperative VAS score,incidence of vascular crisis,hospitalization time,PSQI score,and HAM-A and HAM-D scores of the observation group were lower than those of the control group(P<0.05).Meanwhile,the patient satisfaction of the observation group was higher than that of the control group(P<0.05).Conclusion:Perioperative intensive nursing care for patients undergoing severed finger reimplantation demonstrates significant benefits.It reduces postoperative pain,lowers the incidence of vascular crises,shortens hospitalization durations,enhances sleep quality,alleviates negative emotions,and improves nursing satisfaction.These findings underscore the importance and applicability of such care practices.
文摘This study was conducted to generate information required to guide plantation management in relation to replanting on soils derived from different parent materials. Six oil palm estates in coastal lowlands of southwest Cameroon were considered. Oil palm yield data (in t⋅ha−1 of fresh fruit bunch, FFB) and corresponding age of palms (in years after planting, YAP) were obtained for the various estates. In all the estates, average yields were ⋅FFB⋅ha−1 and highly variable. Plantation age, solely, explained between 20% - 58% of the variation in yield. The highest average yields (11.5 t⋅FFB⋅ha−1) were obtained in plantations aged between 9 and 18 YAP and the lowest (4.66 t⋅FFB⋅ha−1) were obtained in old plantations (>23 YAP). Plantations located on volcanic parent materials generally had higher yields compared to those established on sedimentary parent materials. In order to intensify production and increase yields while conserving the environment, one important measure to consider is the replacement of aged palms, and the recommended optimal replanting age in coastal plains of southwest Cameroon should be at most 23 YAP. Estimated mean yields, if aged palms are replanted on time, can increase by 43% - 65%. Additionally, site-specific nutrient management options should be considered in plantation intensification programs.
文摘Objective: To evaluate the value of inactivated bone replantation with preservation of the epiphysis following the effective chemotherapy in avoiding postoperative discrepancy of the affected limb in children with osteosarcoma. Methods: Two children (aged 5 and 10 years, 1 male and 1 female) with osteosarcoma underwent inactivated bone replantation with preserving epiphysis following chemotherapy (MMIA protocol, including high-dose methotrexate, adriamycin and ifosfamide). After two cycles of preop-erative chemotherapy, pain vanished, the local mass shrank and there was no pain on pressing the affected parts. Sera AKP and LDH were reduced to normal levels; marked shrinkage and sclerotic changes and good margin of lesions were seen on plain radiographs and MR images. Two courses of the same protocol as preoperative chemotherapy were administered postoperatively. Results: Postoperative histological examination of the specimens demonstrated absence of vital tumor cells. Incisions healed well and no complications occurred. The replanted inactivated bone healed with host at 6 months after operation. In the two patients, no evidence was seen of metastasis and recurrence and discrepancy of the affected limbs in postoperative 36 and 48 months. Functions of the affected limbs were satisfactory. Conclusion: Inactivated bone replantation with preserving epiphysis was a viable option for osteosarcoma in children. The long-term outcomes remain to be further proven.
文摘Rangelands contribute to human well-being worldwide.However,its fragile ecosystems are threatened due to inappropriate management that has been leading to its degradation in African rangelands in general and in Ethiopian rangelands in particular.Rangeland degradation is attributable to both natural and anthropogenic causes.Restoring degraded areas by replanting using native species is one of the most promising sustainable rangeland management tools to fight the degradation in the rangelands and enhance resilience in the face of environmental shocks.Restoration improves vegetation cover and biomass yield and enhances other ecosystem services.Native drought-tolerant species have produced promising rehabilitation outcomes and have been recommended for the restoration of degraded rangeland areas.Replanting using native species remains a viable sustainable management option to enhance resilience in the face of environmental shocks.Therefore,to maintain the sustainability and resilient rangeland ecosystems,comprehensive approaches and strategies suitable for rangelands need to be revitalized,developed,strengthened and promoted.
基金funded by the National Science and Technology Support Program of China (2014BAD16B07)
文摘Soil fungi play a very important role in the soil ecological environment. In agricultural production, long-term monoculture and continuous cropping lead to changes in fungal community diversity. However, the effects of long-term monoculture and continuous cropping on strawberry plant health and fungal community diversity have not been elucidated. In this study, using high-throughput sequencing(HTS), we compared the fungal community and diversity of strawberry rhizosphere soil after various durations of continuous cropping(0, 2, 4, 6, 8, 10 and 12 years). The results showed that soil fungal diversity increased with consecutive cropping years. Specifically, the soil-borne disease pathogens Fusarium and Guehomyces were significantly increased after strawberry continuous cropping, and the abundance of nematicidal(Arthrobotrys) fungi decreased from the fourth year of continuous cropping. The results of correlation analysis suggest that these three genera might be key fungi that contribute to the changes in soil properties that occur during continuous cropping. In addition, physicochemical property analysis showed that the soil nutrient content began to decline after the fourth year of continuous cropping. Spearman's correlation analysis showed that soil pH, available potassium(AK) and ammonium nitrogen(NH_4^+-N) were the most important edaphic factors leading to contrasting beneficial and pathogenic associations across consecutive strawberry cropping systems.
基金supported by earmarked fund for National Modern Agro-industry Technology Research System of China(Grant No.CARS-27)Qingchuang Science and Technology Support Project of Shandong Colleges and Universities(Grant No.2019KJF020)+4 种基金Project Supported by Taishan Scholars(Grant No.ts20190923)Shandong Province Agricultural Major Applied Technology Innovation Projects(Grant No.SD2019ZZ008)the National Key Research and Development Program of China(Grant No.2016YFD0201114)the National Natural Science Foundation of China(Grant No.31672104)Fruit Innovation Team in Shandong Province,China(Grant No.SDAIT-06-07).
文摘A two-year field experiment was carried out in order to study the occurrence degree and mechanism of apple replant disease(ARD)in the apple orchards with different soil textures.So we can adopt appropriate controlmeasures according to the severity of ARD.Healthy two-year-old seedlings with consistent growth were selected,of which the root stock was T337 and the scion was Yanfu 3.There were significant differences in biomass between methyl bromide fumigation and replanted treatments,and the difference was the largest in clay loam,followed by sandy loam,and loam,which verified ARD in clay loam was most serious,followed by sandy loam and loam.Based on high-throughput sequencing of fungi in soil samples,fungal richness and diversity were the highest in clay loam,followed by sandy loam,and loam.The relative abundance of Fusarium in SX,SL,FX,FL,WX and WL was 7.33%,19.32%,2.70%,4.24%,10.71%and 23.87%,respectively.Based on Real-time quantitative analysis,there were significant differences in the number of Fusarium oxysporum and Fusarium solani between methyl bromide fumigation and replanted treatments,i.e.,clay loam>sandy loam>loam.Fusarium was the main pathogen causing ARD.This shows that ARD is the most serious under replanted clay loam condition.High-throughput sequencing technology was used to prove the difference in Fusarium was one of the important reasons for ARD under different soil textures.This technology provides a new idea for the prevention and control of ARD.
