BACKGROUND Ependymoma with lipomatous differentiation is a rare type of ependymoma.The ZFTA fusion-positive supratentorial ependymoma is a novel tumor type in the 2021 World Health Organization classification of centr...BACKGROUND Ependymoma with lipomatous differentiation is a rare type of ependymoma.The ZFTA fusion-positive supratentorial ependymoma is a novel tumor type in the 2021 World Health Organization classification of central nervous system tumors.ZFTA fusion-positive lipomatous ependymoma has not been reported to date.CASE SUMMARY We reported a case of a 15-year-old Chinese male who had a sudden convulsion lasting approximately six minutes.Magnetic resonance imaging showed a round cystic shadow of approximately 1.9 cm×1.5 cm×1.9 cm under the right parieto-occipital cortex.Microscopic examination showed characteristic perivascular pseudorosettes and adipose differentiation in the cytoplasm.Immunohisto-chemical staining showed that the tumor cells were negative for cytokeratin,NeuN,Syn and p53,but positive for GFAP,vimentin and S-100 protein.Signi-ficant punctate intracytoplasmic EMA immunoreactivity was observed.The level of Ki-67 was about 5%.Genetic analysis revealed ZFTA:RELA fusion.A cranio-tomy with total excision of the tumor was performed.The follow-up time was 36 months,no evidence of disease recurrence was found in magnetic resonance imaging.CONCLUSION Based on these findings,the patient was diagnosed as a ependymoma with ZFTA fusion and lipomatous differentiation.This case report provides information on the microscopic morphological features of ependymoma with ZFTA fusion and lipomatous differentiation,which can help pathologists to make a definitive diagnosis of this tumor.展开更多
Post-translational modifications(PTMs)regulate the activity and functionality of RELA,but their role in the pathogenesis of liver fibrosis is unclear.This study was performed to understand the regulation mechanism of ...Post-translational modifications(PTMs)regulate the activity and functionality of RELA,but their role in the pathogenesis of liver fibrosis is unclear.This study was performed to understand the regulation mechanism of acetylation of RELA on liver inflammation and fibrosis in a model animal of innate glucose intolerance,largemouth bass,and to provide a potential target and biomarker for liver fibrosis therapy.We found that the acetylation of total proteins and RELA was significantly reduced in fibrotic livers of largemouth bass induced by a high-carbohydrate and high-fat diet(HCHFD)and CCL4 challenge.Furthermore,quantitative acetylome data showed that the K119 site of RELA was deacetylated in fibrotic livers compared to healthy controls.Subsequently,we reveal a new mechanism that SIRT7 deacetylates RELA at the K119 site in largemouth bass.RELA K119 deacetylation enhances RELA transcriptional activity by increasing its DNA-binding activity,and facilitates nuclear translocation of RELA,resulting in the overwhelming release of proinflammatory factors,and subsequently enhancing liver inflammation and fibrosis.Pharmacological inhibition of SIRT7 using a specific inhibitor restores the decreased acetylation of RELA in vivo and in vitro,and reduces the transcriptional activity,nuclear localization of RELA and the expression of its target genes,which ultimately attenuates liver inflammation and fibrosis.These findings uncover a novel mechanism underlying liver fibrosis involving SIRT7-mediated deacetylation of RELA to activate the proinflammatory gene program,and thus provide important insights and biomarkers into the effective strategies for limiting liver inflammation and fibrosis.展开更多
[Objectives]To develop a pair of specific primers for the PCR amplification of the full-length relA gene from Vibrio alginolyticus strain HY9901,as well as to conduct bioinformatics analysis.[Methods]The relA gene was...[Objectives]To develop a pair of specific primers for the PCR amplification of the full-length relA gene from Vibrio alginolyticus strain HY9901,as well as to conduct bioinformatics analysis.[Methods]The relA gene was amplified through PCR,and the resulting gene sequence was subsequently analyzed using bioinformatics tools,including amino acid sequence prediction,functional site analysis,subcellular localization prediction,and homology comparison.[Results]The relA gene had a total length of 2220 bp and encoded 739 amino acid residues.The molecular weight was approximately 84.1261 kDa,and its isoelectric point was 5.95.The protein lacked a signal peptide and transmembrane regions,while exhibiting multiple phosphorylation sites.Predictions regarding its subcellular localization suggested that it was predominantly situated in the cytoplasm.The amino acid sequence demonstrated a homology of 97%to 99%with other species within the genus Vibrio,and it clustered within the same subfamily as V.antiquarius and V.diabolicus.In the prediction of secondary structure,the proportions ofα-helix,extended strand,random coil,andβ-sheet were 54.13%,12.04%,28.15%and 5.68%,respectively.The similarity between the tertiary structure model and template 5kpw.1.w was 66%.[Conclusions]In this study,the relA gene of V.alginolyticus strain HY9901 has been successfully amplified and analyzed.The structural characteristics and potential functions of the encoded protein have been elucidated,thereby providing foundational data for understanding the role of this gene in V.alginolyticus.展开更多
Nowadays, picture fuzzy set theory is a flourishing field in mathematics with uncertainty by incorporating the concept of positive, negative and neutral membership degrees of an object. A traditional crisp relation re...Nowadays, picture fuzzy set theory is a flourishing field in mathematics with uncertainty by incorporating the concept of positive, negative and neutral membership degrees of an object. A traditional crisp relation represents the satisfaction or the dissatisfaction of relationship, connection or correspondence between the objects of two or more sets. However, there are some problems that can’t be solved through classical relationships, such as the relationship between two objects being vague. In those situations, picture fuzzy relation over picture fuzzy sets is an important and powerful concept which is suitable for describing correspondences between two vague objects. It represents the strength of association of the elements of picture fuzzy sets. It plays an important role in picture fuzzy modeling, inference and control system and also has important applications in relational databases, approximate reasoning, preference modeling, medical diagnosis, etc. In this article, we define picture fuzzy relations over picture fuzzy sets, including some other fundamental definitions with illustrations. The max-min and min-max compositions of picture fuzzy relations are defined in the light of picture fuzzy sets and discussed some properties related to them. The reflexivity, symmetry and transitivity of a picture fuzzy relation are described over a picture fuzzy set. Finally, various properties are explored related to the picture fuzzy relations over a picture fuzzy set.展开更多
文摘BACKGROUND Ependymoma with lipomatous differentiation is a rare type of ependymoma.The ZFTA fusion-positive supratentorial ependymoma is a novel tumor type in the 2021 World Health Organization classification of central nervous system tumors.ZFTA fusion-positive lipomatous ependymoma has not been reported to date.CASE SUMMARY We reported a case of a 15-year-old Chinese male who had a sudden convulsion lasting approximately six minutes.Magnetic resonance imaging showed a round cystic shadow of approximately 1.9 cm×1.5 cm×1.9 cm under the right parieto-occipital cortex.Microscopic examination showed characteristic perivascular pseudorosettes and adipose differentiation in the cytoplasm.Immunohisto-chemical staining showed that the tumor cells were negative for cytokeratin,NeuN,Syn and p53,but positive for GFAP,vimentin and S-100 protein.Signi-ficant punctate intracytoplasmic EMA immunoreactivity was observed.The level of Ki-67 was about 5%.Genetic analysis revealed ZFTA:RELA fusion.A cranio-tomy with total excision of the tumor was performed.The follow-up time was 36 months,no evidence of disease recurrence was found in magnetic resonance imaging.CONCLUSION Based on these findings,the patient was diagnosed as a ependymoma with ZFTA fusion and lipomatous differentiation.This case report provides information on the microscopic morphological features of ependymoma with ZFTA fusion and lipomatous differentiation,which can help pathologists to make a definitive diagnosis of this tumor.
基金supported by National Natural Sci-ence Foundation of China(32273141,32202954,32172981)Beijing Natural Science Foundation(6232038).
文摘Post-translational modifications(PTMs)regulate the activity and functionality of RELA,but their role in the pathogenesis of liver fibrosis is unclear.This study was performed to understand the regulation mechanism of acetylation of RELA on liver inflammation and fibrosis in a model animal of innate glucose intolerance,largemouth bass,and to provide a potential target and biomarker for liver fibrosis therapy.We found that the acetylation of total proteins and RELA was significantly reduced in fibrotic livers of largemouth bass induced by a high-carbohydrate and high-fat diet(HCHFD)and CCL4 challenge.Furthermore,quantitative acetylome data showed that the K119 site of RELA was deacetylated in fibrotic livers compared to healthy controls.Subsequently,we reveal a new mechanism that SIRT7 deacetylates RELA at the K119 site in largemouth bass.RELA K119 deacetylation enhances RELA transcriptional activity by increasing its DNA-binding activity,and facilitates nuclear translocation of RELA,resulting in the overwhelming release of proinflammatory factors,and subsequently enhancing liver inflammation and fibrosis.Pharmacological inhibition of SIRT7 using a specific inhibitor restores the decreased acetylation of RELA in vivo and in vitro,and reduces the transcriptional activity,nuclear localization of RELA and the expression of its target genes,which ultimately attenuates liver inflammation and fibrosis.These findings uncover a novel mechanism underlying liver fibrosis involving SIRT7-mediated deacetylation of RELA to activate the proinflammatory gene program,and thus provide important insights and biomarkers into the effective strategies for limiting liver inflammation and fibrosis.
基金Supported by Natural Science Foundation of Guangdong Province(2025A1515011061)Outstanding Graduate Entering Laboratory Project of College of Fisheries,Guangdong Ocean University+1 种基金Undergraduate Innovation and Entrepreneurship Training Program of Guangdong Ocean University(CXXL2024007)Undergraduate Innovation Team of Guangdong Ocean University(CCTD201802).
文摘[Objectives]To develop a pair of specific primers for the PCR amplification of the full-length relA gene from Vibrio alginolyticus strain HY9901,as well as to conduct bioinformatics analysis.[Methods]The relA gene was amplified through PCR,and the resulting gene sequence was subsequently analyzed using bioinformatics tools,including amino acid sequence prediction,functional site analysis,subcellular localization prediction,and homology comparison.[Results]The relA gene had a total length of 2220 bp and encoded 739 amino acid residues.The molecular weight was approximately 84.1261 kDa,and its isoelectric point was 5.95.The protein lacked a signal peptide and transmembrane regions,while exhibiting multiple phosphorylation sites.Predictions regarding its subcellular localization suggested that it was predominantly situated in the cytoplasm.The amino acid sequence demonstrated a homology of 97%to 99%with other species within the genus Vibrio,and it clustered within the same subfamily as V.antiquarius and V.diabolicus.In the prediction of secondary structure,the proportions ofα-helix,extended strand,random coil,andβ-sheet were 54.13%,12.04%,28.15%and 5.68%,respectively.The similarity between the tertiary structure model and template 5kpw.1.w was 66%.[Conclusions]In this study,the relA gene of V.alginolyticus strain HY9901 has been successfully amplified and analyzed.The structural characteristics and potential functions of the encoded protein have been elucidated,thereby providing foundational data for understanding the role of this gene in V.alginolyticus.
文摘Nowadays, picture fuzzy set theory is a flourishing field in mathematics with uncertainty by incorporating the concept of positive, negative and neutral membership degrees of an object. A traditional crisp relation represents the satisfaction or the dissatisfaction of relationship, connection or correspondence between the objects of two or more sets. However, there are some problems that can’t be solved through classical relationships, such as the relationship between two objects being vague. In those situations, picture fuzzy relation over picture fuzzy sets is an important and powerful concept which is suitable for describing correspondences between two vague objects. It represents the strength of association of the elements of picture fuzzy sets. It plays an important role in picture fuzzy modeling, inference and control system and also has important applications in relational databases, approximate reasoning, preference modeling, medical diagnosis, etc. In this article, we define picture fuzzy relations over picture fuzzy sets, including some other fundamental definitions with illustrations. The max-min and min-max compositions of picture fuzzy relations are defined in the light of picture fuzzy sets and discussed some properties related to them. The reflexivity, symmetry and transitivity of a picture fuzzy relation are described over a picture fuzzy set. Finally, various properties are explored related to the picture fuzzy relations over a picture fuzzy set.
